Restriction map for
pFN21AB8301
(Circular) (Six-base) MAPSORT of: pFN21AB8301.seq Check: 9746 from: 1 to: 6139
pFN21AB8301 6139 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2578 2578
Size: 6139
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2376 3351 3358 3482 1937
Size: 439 975 7 124 4594
Fragments arranged by size:
4594 975 439 124 7
Acc65I G'GTAC_C
Cuts at: 3460 3460
Size: 6139
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4202 5586 5854 1632
Size: 2570 1384 268 1917
Fragments arranged by size:
2570 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 2553 5038 6015 1203
Size: 1350 2485 977 1327
Fragments arranged by size:
2485 1350 1327 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 3365 4868 3365
Size: 1503 4636
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2423 1184
Size: 1239 4900
AlwNI CAG_nnn'CTG
Cuts at: 2015 2644 2745 3430 3882 5454 2015
Size: 629 101 685 452 1572 2700
Fragments arranged by size:
2700 1572 685 629 452 101
ApaLI G'TGCA_C
Cuts at: 4196 5352 4196
Size: 1156 4983
ApoI r'AATT_y
Cuts at: 1493 3448 3674 3826 5935 1493
Size: 1955 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 2469 3073 3464 1870
Size: 73 526 604 391 4545
Fragments arranged by size:
4545 604 526 391 73
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3460 4915 618
Size: 325 217 627 1673 1455 1842
Fragments arranged by size:
1842 1673 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2240 2508 3058 3458 1391
Size: 849 268 550 400 4072
Fragments arranged by size:
4072 849 550 400 268
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2273 2673 928
Size: 971 374 400 4394
Fragments arranged by size:
4394 971 400 374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3072 3106 4330 4364 1903
Size: 34 1135 34 1224 34 3678
Fragments arranged by size:
3678 1224 1135 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2135 4750 136
Size: 107 122 71 631 605 463 2615 1525
Fragments arranged by size:
2615 1525 631 605 463 122 107 71
BglII A'GATC_T
Cuts at: 6134 6134
Size: 6139
BlpI GC'TnA_GC
Cuts at: 3020 3556 3020
Size: 536 5603
Bme1580I G_kGCm'C
Cuts at: 1790 2173 2625 3286 4200 5356 1790
Size: 383 452 661 914 1156 2573
Fragments arranged by size:
2573 1156 914 661 452 383
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2755 2992 3273
Size: 416 53 83 399 24 1338 237 281
Cuts at: 3273 3870 4828 442
Size: 597 958 1753
Fragments arranged by size:
1753 1338 958 597 416 399 281 237
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2996 1056
Size: 1940 4199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2478 2654 2918 3288 4799 1239
Size: 1239 176 264 370 1511 2579
Fragments arranged by size:
2579 1511 1239 370 264 176
Bpu10I CC'TnA_GC
Cuts at: 2192 2276 2192
Size: 84 6055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2581 3080 3089 3259 4264 5129 5427 2581
Size: 499 9 170 1005 865 298 3293
Fragments arranged by size:
3293 1005 865 499 298 170 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3207 4802 882
Size: 617 1708 1595 2219
Fragments arranged by size:
2219 1708 1595 617
BsaAI yAC'GTr
Cuts at: 493 1204 3930 493
Size: 711 2726 2702
Fragments arranged by size:
2726 2702 711
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3946 4328 4963 275
Size: 53 83 186 936 2413 382 635 1451
Fragments arranged by size:
2413 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2433 2508 3205 4572 4970 5244 5391 5802 2433
Size: 75 697 1367 398 274 147 411 2770
Fragments arranged by size:
2770 1367 697 411 398 274 147 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2891 2921 1178
Size: 30 165 30 1488 30 4396
Fragments arranged by size:
4396 1488 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2299 3203 1186
Size: 1113 904 4122
Fragments arranged by size:
4122 1113 904
BseYI C'CCAG_C
Cuts at: 2095 2119 2214 5342 5838 2095
Size: 24 95 3128 496 2396
Fragments arranged by size:
3128 2396 496 95 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2924 1163
Size: 573 1188 4378
Fragments arranged by size:
4378 1188 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2158 3223 3589 4350 5378 1540
Size: 275 186 157 1065 366 761 1028 2301
Fragments arranged by size:
2301 1065 1028 761 366 275 186 157
BsiHKAI G_wGCw'C
Cuts at: 2076 2508 4200 4285 5356 2076
Size: 432 1692 85 1071 2859
Fragments arranged by size:
2859 1692 1071 432 85
BsmI GAATG_Cn'
Cuts at: 3650 3743 3650
Size: 93 6046
BsmBI CGTCTCn'nnnn_
Cuts at: 3032 3032
Size: 6139
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2173 2240 2508 2625 3058 3286
Size: 399 286 97 67 268 117 433 228
Cuts at: 3286 3458 4200 4285 5356 1391
Size: 172 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 286 268 228
172 117 97 85 67
BspEI T'CCGG_A
Cuts at: 2508 3205 5802 2508
Size: 697 2597 2845
Fragments arranged by size:
2845 2597 697
BspHI T'CATG_A
Cuts at: 2676 4028 5758 2676
Size: 1352 1730 3057
Fragments arranged by size:
3057 1730 1352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2578 2592 3494 844
Size: 1734 14 902 3489
Fragments arranged by size:
3489 1734 902 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2111 4026 1394
Size: 429 288 1915 3507
Fragments arranged by size:
3507 1915 429 288
BsrDI GCAATG_nn'
Cuts at: 65 1248 4634 4808 65
Size: 1183 3386 174 1396
Fragments arranged by size:
3386 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2280 2988 4783 4970 1700
Size: 110 470 708 1795 187 2869
Fragments arranged by size:
2869 1795 708 470 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6139
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 2730 4199 5211 2730
Size: 1469 1012 3658
Fragments arranged by size:
3658 1469 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6139
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6139
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2356 2233
Size: 123 6016
BstYI r'GATC_y
Cuts at: 1972 3099 3469 3836 4221 4238 5690 5776 5788
Size: 1127 370 367 385 17 1452 86 12
Cuts at: 5788 6134 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1127 385 370 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3033 513
Size: 552 939 1029 3619
Fragments arranged by size:
3619 1029 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2110 487
Size: 683 56 884 4516
Fragments arranged by size:
4516 884 683 56
BtsI GCAGTG_nn'
Cuts at: 3144 3655 4449 4469 3144
Size: 511 794 20 4814
Fragments arranged by size:
4814 794 511 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2112 2358
Size: 54 1425 50 275 18 228 54 246
Cuts at: 2358 2831 2976 3216 3404 3586 4475 6104 8
Size: 473 145 240 188 182 889 1629 43
Fragments arranged by size:
1629 1425 889 473 275 246 240 228
188 182 145 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3586 1537
Size: 275 1774 4090
Fragments arranged by size:
4090 1774 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2933 4069 1353
Size: 1580 1136 3423
Fragments arranged by size:
3423 1580 1136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2855 4726 5100 5246 387
Size: 1064 138 1266 1871 374 146 1280
Fragments arranged by size:
1871 1280 1266 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2506 2506
Size: 6139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2478 2654 2918 3288 4202 4799 5586
Size: 393 846 176 264 370 914 597 787
Cuts at: 5586 5854 1239
Size: 268 1524
Fragments arranged by size:
1524 914 846 787 597 393 370 268
264 176
EcoO109I rG'GnC_Cy
Cuts at: 2844 3427 2844
Size: 583 5556
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
EcoRV GAT'ATC
Cuts at: 3329 3329
Size: 6139
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 2016 5286 1678
Size: 338 3270 2531
Fragments arranged by size:
3270 2531 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2140 2172 3013 3045 3351
Size: 32 135 32 568 32 841 32 306
Cuts at: 3351 3383 4780 4812 4854 4886 1373
Size: 32 1397 32 42 32 2626
Fragments arranged by size:
2626 1397 841 568 306 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3359 3483 3729 669
Size: 988 281 1421 124 246 3079
Fragments arranged by size:
3079 1421 988 281 246 124
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6139
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KpnI G_GTAC'C
Cuts at: 3464 3464
Size: 6139
MfeI C'AATT_G
Cuts at: 2827 3738 2827
Size: 911 5228
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2635 5253 5437 1305
Size: 1330 2618 184 2007
Fragments arranged by size:
2618 2007 1330 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 2833 2978 3406 10
Size: 54 1425 343 528 473 145 428 2743
Fragments arranged by size:
2743 1425 528 473 428 343 145 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2675 4097 4456 4615 518
Size: 648 1509 1422 359 159 2042
Fragments arranged by size:
2042 1509 1422 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2600 2754 3035 3372 3555 4232 5380 5625
Size: 994 154 281 337 183 677 1148 245
Cuts at: 5625 1606
Size: 2120
Fragments arranged by size:
2120 1148 994 677 337 281 245 183
154
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4648
Fragments arranged by size:
4648 939 552
NdeI CA'TA_TG
Cuts at: 387 2495 3113 387
Size: 2108 618 3413
Fragments arranged by size:
3413 2108 618
NheI G'CTAG_C
Cuts at: 1052 2992 1052
Size: 1940 4199
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 2404 2542 2557 3497 5042 1132
Size: 97 1175 138 15 940 1545 2229
Fragments arranged by size:
2229 1545 1175 940 138 97 15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 2553 5038 2553
Size: 2485 3654
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2829 1108
Size: 1721 4418
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2844 2844
Size: 6139
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PstI C_TGCA'G
Cuts at: 830 2412 2589 3234 3491 830
Size: 1582 177 645 257 3478
Fragments arranged by size:
3478 1582 645 257 177
PvuI CG_AT'CG
Cuts at: 2001 2158 2001
Size: 157 5982
PvuII CAG'CTG
Cuts at: 2600 3372 2600
Size: 772 5367
SacI G_AGCT'C
Cuts at: 2508 2508
Size: 6139
SacII CC_GC'GG
Cuts at: 3036 3036
Size: 6139
SalI G'TCGA_C
Cuts at: 1936 3357 3481 1936
Size: 1421 124 4594
Fragments arranged by size:
4594 1421 124
SanDI GG'GwC_CC
Cuts at: 2844 2844
Size: 6139
SbfI CC_TGCA'GG
Cuts at: 2412 2589 3234 3491 2412
Size: 177 645 257 5060
Fragments arranged by size:
5060 645 257 177
ScaI AGT'ACT
Cuts at: 2723 4387 6099 2723
Size: 1664 1712 2763
Fragments arranged by size:
2763 1712 1664
SfcI C'TryA_G
Cuts at: 826 1045 2408 2585 3230 3487 4622 5303 5494
Size: 219 1363 177 645 257 1135 681 191
Cuts at: 5494 826
Size: 1471
Fragments arranged by size:
1471 1363 1135 681 645 257 219 191
177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 3466 1872
Size: 1594 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2560 3095 3104 3238 4243 5144
Size: 197 926 617 535 9 134 1005 901
Cuts at: 5144 5406 820
Size: 262 1553
Fragments arranged by size:
1553 1005 926 901 617 535 262 197
134 9
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 3388 152
Size: 3236 2903
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 3236 3305 1517
Size: 1719 69 4351
Fragments arranged by size:
4351 1719 69
StyI C'CwwG_G
Cuts at: 513 1065 2004 3294 3578 513
Size: 552 939 1290 284 3074
Fragments arranged by size:
3074 1290 939 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2093 4166 4183 4336 4521 4966 325
Size: 1255 513 2073 17 153 185 445 1498
Fragments arranged by size:
2073 1498 1255 513 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2556 2721 4385
Size: 274 80 33 51 1443 579 165 1664
Cuts at: 4385 6097 96
Size: 1712 138
Fragments arranged by size:
1712 1664 1443 579 274 165 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6099 6131 6099
Size: 32 6107
Tth111I GACn'n_nGTC
Cuts at: 1316 2966 1316
Size: 1650 4489
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2086 3130 3389 2086
Size: 1044 259 4836
Fragments arranged by size:
4836 1044 259
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 3464 1870
Size: 1594 4545
XmnI GAAnn'nnTTC
Cuts at: 4268 4268
Size: 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SapI
SexAI SfiI SfoI SrfI SwaI