Restriction map for
pFN21AB8299
(Circular) (Six-base) MAPSORT of: pFN21AB8299.seq Check: 2370 from: 1 to: 6316
pFN21AB8299 6316 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4126 278
Size: 53 83 186 936 2590 2468
Fragments arranged by size:
2590 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3659 1937
Size: 1722 4594
Acc65I G'GTAC_C
Cuts at: 3637 3637
Size: 6316
AclI AA'CG_TT
Cuts at: 1563 4443 4816 1563
Size: 2880 373 3063
Fragments arranged by size:
3063 2880 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2637 3005 3080 4379 5763 6031 1632
Size: 1005 368 75 1299 1384 268 1917
Fragments arranged by size:
1917 1384 1299 1005 368 268 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6119
AflIII A'CryG_T
Cuts at: 1203 5215 6192 1203
Size: 4012 977 1327
Fragments arranged by size:
4012 1327 977
AgeI A'CCGG_T
Cuts at: 5147 5147
Size: 6316
AhdI GACnn_n'nnGTC
Cuts at: 5045 5045
Size: 6316
AleI CACnn'nnGTG
Cuts at: 1166 2534 3182 1166
Size: 1368 648 4300
Fragments arranged by size:
4300 1368 648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2299 2393 2552 1184
Size: 1115 94 159 4948
Fragments arranged by size:
4948 1115 159 94
AlwNI CAG_nnn'CTG
Cuts at: 2269 2404 2548 2560 3091 4059 5631 2269
Size: 135 144 12 531 968 1572 2954
Fragments arranged by size:
2954 1572 968 531 144 135 12
ApaLI G'TGCA_C
Cuts at: 4373 5529 4373
Size: 1156 5160
ApoI r'AATT_y
Cuts at: 1493 3625 3851 4003 6112 1493
Size: 2132 226 152 2109 1697
Fragments arranged by size:
2132 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4870 160
Size: 872 3838 1606
Fragments arranged by size:
3838 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2226 3314 3531 3641 1870
Size: 73 70 213 1088 217 110 4545
Fragments arranged by size:
4545 1088 217 213 110 73 70
BamHI G'GATC_C
Cuts at: 3646 4013 3646
Size: 367 5949
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3637 5092 618
Size: 325 217 627 1850 1455 1842
Fragments arranged by size:
1850 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 3635 1391
Size: 622 1622 4072
Fragments arranged by size:
4072 1622 622
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2739 928
Size: 971 840 4505
Fragments arranged by size:
4505 971 840
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4507 4541 1903
Size: 34 2570 34 3678
Fragments arranged by size:
3678 2570 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4208 5418 4208
Size: 1210 5106
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6316
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4927 136
Size: 107 122 71 631 605 3255 1525
Fragments arranged by size:
3255 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6311 6311
Size: 6316
BlpI GC'TnA_GC
Cuts at: 2381 3008 3733 2381
Size: 627 725 4964
Fragments arranged by size:
4964 725 627
Bme1580I G_kGCm'C
Cuts at: 1790 2941 3031 3237 4377 5533 1790
Size: 1151 90 206 1140 1156 2573
Fragments arranged by size:
2573 1156 1151 1140 206 90
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6316
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4047 5005 442
Size: 416 53 83 399 24 2630 958 1753
Fragments arranged by size:
2630 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6316
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3015 3015
Size: 6316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 2612 2817 2903 3478 4976 1239
Size: 1130 243 205 86 575 1498 2579
Fragments arranged by size:
2579 1498 1130 575 243 205 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2840 4441 5306 5604 2342
Size: 498 1601 865 298 3054
Fragments arranged by size:
3054 1601 865 498 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2161 2793 4979 882
Size: 617 662 632 2186 2219
Fragments arranged by size:
2219 2186 662 632 617
BsaAI yAC'GTr
Cuts at: 493 1204 4107 493
Size: 711 2903 2702
Fragments arranged by size:
2903 2702 711
BsaBI GATnn'nnATC
Cuts at: 6310 6310
Size: 6316
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4123 4505 5140 275
Size: 53 83 186 936 2590 382 635 1451
Fragments arranged by size:
2590 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2130 4749 5147 5421 5568 5979 2130
Size: 2619 398 274 147 411 2467
Fragments arranged by size:
2619 2467 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2622 2652 3476 3506 1178
Size: 30 165 30 1219 30 824 30 3988
Fragments arranged by size:
3988 1219 824 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2479 2495 2825 2864 2933 3419 3455
Size: 874 419 16 330 39 69 486 36
Cuts at: 3455 3580 1186
Size: 125 3922
Fragments arranged by size:
3922 874 486 419 330 125 69 39
36 16
BseYI C'CCAG_C
Cuts at: 2602 3043 5519 6015 2602
Size: 441 2476 496 2903
Fragments arranged by size:
2903 2476 496 441
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5743
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3766 4527 5555 1540
Size: 275 186 1765 761 1028 2301
Fragments arranged by size:
2301 1765 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 2382 2688 3224 3456 4377 4462 5533 2091
Size: 291 306 536 232 921 85 1071 2874
Fragments arranged by size:
2874 1071 921 536 306 291 232 85
BsmI GAATG_Cn'
Cuts at: 3271 3827 3920 3271
Size: 556 93 5667
Fragments arranged by size:
5667 556 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2030 2030
Size: 6316
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2091 2382 2688 2941 3031 3224
Size: 399 223 78 291 306 253 90 193
Cuts at: 3224 3237 3456 3635 4377 4462 5533 1391
Size: 13 219 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 291 253 223
219 193 179 90 85 78 13
BspEI T'CCGG_A
Cuts at: 5979 5979
Size: 6316
BspHI T'CATG_A
Cuts at: 3162 4205 5935 3162
Size: 1043 1730 3543
Fragments arranged by size:
3543 1730 1043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3671 844
Size: 2827 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2662 4203 1394
Size: 429 839 1541 3507
Fragments arranged by size:
3507 1541 839 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4811 4985 65
Size: 1183 3563 174 1396
Fragments arranged by size:
3563 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3309 4960 5147 1700
Size: 110 1499 1651 187 2869
Fragments arranged by size:
2869 1651 1499 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6316
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6316
BssSI C'ACGA_G
Cuts at: 2375 3455 4376 5388 2375
Size: 1080 921 1012 3303
Fragments arranged by size:
3303 1080 1012 921
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6316
BstBI TT'CG_AA
Cuts at: 5120 5120
Size: 6316
BstEII G'GTnAC_C
Cuts at: 1184 3472 1184
Size: 2288 4028
BstXI CCAn_nnnn'nTGG
Cuts at: 3469 3469
Size: 6316
BstYI r'GATC_y
Cuts at: 1972 2673 3646 4013 4398 4415 5867 5953 5965
Size: 701 973 367 385 17 1452 86 12
Cuts at: 5965 6311 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 973 701 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3123 3123
Size: 6316
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2667 2818 2901 513
Size: 552 939 27 636 151 83 3928
Fragments arranged by size:
3928 939 636 552 151 83 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5577
Fragments arranged by size:
5577 683 56
BtsI GCAGTG_nn'
Cuts at: 2835 3832 4626 4646 2835
Size: 997 794 20 4505
Fragments arranged by size:
4505 997 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6316
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6281
DraI TTT'AAA
Cuts at: 3620 3620
Size: 6316
DraIII CAC_nnn'GTG
Cuts at: 2779 2830 2779
Size: 51 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2300 5323 809
Size: 507 984 3023 1802
Fragments arranged by size:
3023 1802 984 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2821 3074 3763
Size: 54 1425 50 275 18 991 253 689
Cuts at: 3763 4652 6281 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 991 889 689 275 253 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3763 1537
Size: 275 1951 4090
Fragments arranged by size:
4090 1951 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 2513 2693 3182 4246 1353
Size: 776 384 180 489 1064 3423
Fragments arranged by size:
3423 1064 776 489 384 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2159 3146 4903 5277 5423 387
Size: 1064 138 570 987 1757 374 146 1280
Fragments arranged by size:
1757 1280 1064 987 570 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2612 2637 2817 2903 3005 3080
Size: 393 737 243 25 180 86 102 75
Cuts at: 3080 3478 4379 4976 5763 6031 1239
Size: 398 901 597 787 268 1524
Fragments arranged by size:
1524 901 787 737 597 398 393 268
243 180 102 86 75 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2168 2168
Size: 6316
EcoO109I rG'GnC_Cy
Cuts at: 2760 3292 2760
Size: 532 5784
EcoRI G'AATT_C
Cuts at: 3625 4003 3625
Size: 378 5938
FseI GG_CCGG'CC
Cuts at: 3313 3313
Size: 6316
FspI TGC'GCA
Cuts at: 4822 4822
Size: 6316
HaeII r_GCGC'y
Cuts at: 1678 5463 1678
Size: 3785 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3476 3508 3587 3619 4957
Size: 32 135 32 1904 32 79 32 1338
Cuts at: 4957 4989 5031 5063 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1904 1338 135 79 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3660 3906 669
Size: 988 281 1722 246 3079
Fragments arranged by size:
3079 1722 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3149 748
Size: 2401 3915
HpaI GTT'AAC
Cuts at: 3906 3906
Size: 6316
KpnI G_GTAC'C
Cuts at: 3641 3641
Size: 6316
MfeI C'AATT_G
Cuts at: 3915 3915
Size: 6316
MluI A'CGCG_T
Cuts at: 6192 6192
Size: 6316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5430 5614 1305
Size: 4125 184 2007
Fragments arranged by size:
4125 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3076 10
Size: 54 1425 343 1244 3250
Fragments arranged by size:
3250 1425 1244 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 2915 3182 4274 4633 4792 518
Size: 648 1368 381 267 1092 359 159 2042
Fragments arranged by size:
2042 1368 1092 648 381 359 267 159
MspA1I CmG'CkG
Cuts at: 1606 2548 3732 4409 5557 5802 1606
Size: 942 1184 677 1148 245 2120
Fragments arranged by size:
2120 1184 1148 942 677 245
NaeI GCC'GGC
Cuts at: 3311 3311
Size: 6316
NcoI C'CATG_G
Cuts at: 513 1065 2004 2667 513
Size: 552 939 663 4162
Fragments arranged by size:
4162 939 663 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6316
NgoMIV G'CCGG_C
Cuts at: 3309 3309
Size: 6316
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6316
NotI GC'GGCC_GC
Cuts at: 3763 3763
Size: 6316
NspI r_CATG'y
Cuts at: 1132 1229 3674 5219 1132
Size: 97 2445 1545 2229
Fragments arranged by size:
2445 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3067 1348
Size: 1719 4597
PciI A'CATG_T
Cuts at: 5215 5215
Size: 6316
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3517 1108
Size: 2409 3907
PfoI T'CCnGG_A
Cuts at: 2163 3494 2163
Size: 1331 4985
PmeI GTTT'AAAC
Cuts at: 3620 3620
Size: 6316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4385 4417 5898 5930 4385
Size: 32 1481 32 4771
Fragments arranged by size:
4771 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2760 2760
Size: 6316
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6316
PsiI TTA'TAA
Cuts at: 3886 3886
Size: 6316
PstI C_TGCA'G
Cuts at: 830 2208 2274 3555 3668 830
Size: 1378 66 1281 113 3478
Fragments arranged by size:
3478 1378 1281 113 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6316
PvuII CAG'CTG
Cuts at: 2548 2548
Size: 6316
SalI G'TCGA_C
Cuts at: 1936 3658 1936
Size: 1722 4594
SanDI GG'GwC_CC
Cuts at: 2760 2760
Size: 6316
SapI GCTCTTCn'nnn_
Cuts at: 2693 2693
Size: 6316
SbfI CC_TGCA'GG
Cuts at: 2274 3555 3668 2274
Size: 1281 113 4922
Fragments arranged by size:
4922 1281 113
ScaI AGT'ACT
Cuts at: 3326 4564 6276 3326
Size: 1238 1712 3366
Fragments arranged by size:
3366 1712 1238
SfcI C'TryA_G
Cuts at: 826 1045 2204 2270 3551 3664 4799 5480 5671
Size: 219 1159 66 1281 113 1135 681 191
Cuts at: 5671 826
Size: 1471
Fragments arranged by size:
1471 1281 1159 1135 681 219 191 113
66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6316
SmaI CCC'GGG
Cuts at: 1872 2015 2228 3533 3643 1872
Size: 143 213 1305 110 4545
Fragments arranged by size:
4545 1305 213 143 110
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2357 2855 4420 5321 5583 820
Size: 197 926 414 498 1565 901 262 1553
Fragments arranged by size:
1565 1553 926 901 498 414 262 197
SnaBI TAC'GTA
Cuts at: 493 4107 493
Size: 3614 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6316
SphI G_CATG'C
Cuts at: 1132 3674 1132
Size: 2542 3774
SspI AAT'ATT
Cuts at: 5 52 4240 5
Size: 47 4188 2081
Fragments arranged by size:
4188 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6316
StyI C'CwwG_G
Cuts at: 513 1065 2004 2667 3755 513
Size: 552 939 663 1088 3074
Fragments arranged by size:
3074 1088 939 663 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2647 4343 4360 4513 4698 5143 325
Size: 1255 1067 1696 17 153 185 445 1498
Fragments arranged by size:
1696 1498 1255 1067 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2344 3249 3324
Size: 274 80 33 51 1443 367 905 75
Cuts at: 3324 3357 4562 6274 96
Size: 33 1205 1712 138
Fragments arranged by size:
1712 1443 1205 905 367 274 138 80
75 51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2622 2626 2654 2658 3548 3580 6276 6308 2622
Size: 4 28 4 890 32 2696 32 2630
Fragments arranged by size:
2696 2630 890 32 32 28 4 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6316
XbaI T'CTAG_A
Cuts at: 3652 3652
Size: 6316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2908 3295 2908
Size: 387 5929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6316
XmaI C'CCGG_G
Cuts at: 1870 2013 2226 3531 3641 1870
Size: 143 213 1305 110 4545
Fragments arranged by size:
4545 1305 213 143 110
XmnI GAAnn'nnTTC
Cuts at: 4445 4445
Size: 6316
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4124 276
Size: 53 83 186 936 2590 2468
Fragments arranged by size:
2590 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI
SrfI SwaI