Restriction map for
pFN21AB8292
(Circular) (Six-base) MAPSORT of: pFN21AB8292.seq Check: 4691 from: 1 to: 6052
pFN21AB8292 6052 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3862 278
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3395 1937
Size: 1458 4594
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4115 5499 5767 1632
Size: 2483 1384 268 1917
Fragments arranged by size:
2483 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5855
AflIII A'CryG_T
Cuts at: 1203 2280 3096 4951 5928 1203
Size: 1077 816 1855 977 1327
Fragments arranged by size:
1855 1327 1077 977 816
AgeI A'CCGG_T
Cuts at: 4883 4883
Size: 6052
AhdI GACnn_n'nnGTC
Cuts at: 2569 2698 4781 2569
Size: 129 2083 3840
Fragments arranged by size:
3840 2083 129
AleI CACnn'nnGTG
Cuts at: 1166 2157 3099 1166
Size: 991 942 4119
Fragments arranged by size:
4119 991 942
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6052
AlwNI CAG_nnn'CTG
Cuts at: 3331 3795 5367 3331
Size: 464 1572 4016
Fragments arranged by size:
4016 1572 464
ApaLI G'TGCA_C
Cuts at: 2932 4109 5265 2932
Size: 1177 1156 3719
Fragments arranged by size:
3719 1177 1156
ApoI r'AATT_y
Cuts at: 1493 2105 2141 3361 3587 3739 5848 1493
Size: 612 36 1220 226 152 2109 1697
Fragments arranged by size:
2109 1697 1220 612 226 152 36
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 2551 3377 1870
Size: 73 608 826 4545
Fragments arranged by size:
4545 826 608 73
BamHI G'GATC_C
Cuts at: 3382 3749 3382
Size: 367 5685
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3373 4828 618
Size: 325 217 627 1586 1455 1842
Fragments arranged by size:
1842 1586 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3371 1391
Size: 1980 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2861 3154 928
Size: 971 962 293 3826
Fragments arranged by size:
3826 971 962 293
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4243 4277 1903
Size: 34 2306 34 3678
Fragments arranged by size:
3678 2306 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2363 3944 5154 2363
Size: 1581 1210 3261
Fragments arranged by size:
3261 1581 1210
BclI T'GATC_A
Cuts at: 1548 2053 1548
Size: 505 5547
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2843 4663 136
Size: 107 122 71 631 605 1171 1820 1525
Fragments arranged by size:
1820 1525 1171 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6047 6047
Size: 6052
BlpI GC'TnA_GC
Cuts at: 3469 3469
Size: 6052
Bme1580I G_kGCm'C
Cuts at: 1790 2936 3040 4113 5269 1790
Size: 1146 104 1073 1156 2573
Fragments arranged by size:
2573 1156 1146 1073 104
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6052
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3258 3783 4741
Size: 416 53 83 399 24 1841 525 958
Cuts at: 4741 442
Size: 1753
Fragments arranged by size:
1841 1753 958 525 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3353 4712 1239
Size: 2114 1359 2579
Fragments arranged by size:
2579 2114 1359
Bpu10I CC'TnA_GC
Cuts at: 2588 2588
Size: 6052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2228 2697 3116 4177 5042 5340 2228
Size: 469 419 1061 865 298 2940
Fragments arranged by size:
2940 1061 865 469 419 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2669 4715 882
Size: 617 1170 2046 2219
Fragments arranged by size:
2219 2046 1170 617
BsaAI yAC'GTr
Cuts at: 493 1204 3843 493
Size: 711 2639 2702
Fragments arranged by size:
2702 2639 711
BsaBI GATnn'nnATC
Cuts at: 6046 6046
Size: 6052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3859 4241 4876 275
Size: 53 83 186 936 2326 382 635 1451
Fragments arranged by size:
2326 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4485 4883 5157 5304 5715 4485
Size: 398 274 147 411 4822
Fragments arranged by size:
4822 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5827
Fragments arranged by size:
5827 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6052
BseYI C'CCAG_C
Cuts at: 2617 5255 5751 2617
Size: 2638 496 2918
Fragments arranged by size:
2918 2638 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3169 1163
Size: 573 1433 4046
Fragments arranged by size:
4046 1433 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4263 5291 1540
Size: 275 186 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2152 2936 4113 4198 5269 2152
Size: 784 1177 85 1071 2935
Fragments arranged by size:
2935 1177 1071 784 85
BsmI GAATG_Cn'
Cuts at: 3563 3656 3563
Size: 93 5959
BsmBI CGTCTCn'nnnn_
Cuts at: 3329 3329
Size: 6052
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2936 3040 3371 4113 4198 5269
Size: 399 362 784 104 331 742 85 1071
Cuts at: 5269 1391
Size: 2174
Fragments arranged by size:
2174 1071 784 742 399 362 331 104
85
BspEI T'CCGG_A
Cuts at: 5715 5715
Size: 6052
BspHI T'CATG_A
Cuts at: 3139 3941 5671 3139
Size: 802 1730 3520
Fragments arranged by size:
3520 1730 802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3244 3407 844
Size: 2400 163 3489
Fragments arranged by size:
3489 2400 163
BsrBI CCG'CTC
Cuts at: 1394 1823 3939 1394
Size: 429 2116 3507
Fragments arranged by size:
3507 2116 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4547 4721 65
Size: 1183 3299 174 1396
Fragments arranged by size:
3299 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4696 4883 1700
Size: 110 2886 187 2869
Fragments arranged by size:
2886 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2283 96
Size: 2187 3865
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 3224 4112 5124 3224
Size: 888 1012 4152
Fragments arranged by size:
4152 1012 888
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6052
BstBI TT'CG_AA
Cuts at: 4856 4856
Size: 6052
BstEII G'GTnAC_C
Cuts at: 1184 2816 3256 1184
Size: 1632 440 3980
Fragments arranged by size:
3980 1632 440
BstXI CCAn_nnnn'nTGG
Cuts at: 2849 2849
Size: 6052
BstYI r'GATC_y
Cuts at: 1972 3382 3749 4134 4151 5603 5689 5701 6047
Size: 1410 367 385 17 1452 86 12 346
Cuts at: 6047 1972
Size: 1977
Fragments arranged by size:
1977 1452 1410 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2670 513
Size: 552 939 666 3895
Fragments arranged by size:
3895 939 666 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3320 487
Size: 683 56 2094 3219
Fragments arranged by size:
3219 2094 683 56
BtsI GCAGTG_nn'
Cuts at: 3568 4362 4382 3568
Size: 794 20 5238
Fragments arranged by size:
5238 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6017
DraI TTT'AAA
Cuts at: 2188 3356 2188
Size: 1168 4884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5059 809
Size: 507 3743 1802
Fragments arranged by size:
3743 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2653 3499 4388
Size: 54 1425 50 275 18 823 846 889
Cuts at: 4388 6017 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 846 823 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 3982 1353
Size: 906 1723 3423
Fragments arranged by size:
3423 1723 906
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2921 4639 5013 5159 387
Size: 1064 138 1332 1718 374 146 1280
Fragments arranged by size:
1718 1332 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3353 4115 4712 5499 5767 1239
Size: 393 1721 762 597 787 268 1524
Fragments arranged by size:
1721 1524 787 762 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2269 2700 2269
Size: 431 5621
EcoRI G'AATT_C
Cuts at: 3361 3739 3361
Size: 378 5674
FspI TGC'GCA
Cuts at: 4558 4558
Size: 6052
HaeII r_GCGC'y
Cuts at: 1678 5199 1678
Size: 3521 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2555 2587 2684 2716 4693
Size: 32 135 32 983 32 97 32 1977
Cuts at: 4693 4725 4767 4799 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1977 983 135 97 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2320 3396 3642 669
Size: 988 281 382 1076 246 3079
Fragments arranged by size:
3079 1076 988 382 281 246
HindIII A'AGCT_T
Cuts at: 748 2192 2204 748
Size: 1444 12 4596
Fragments arranged by size:
4596 1444 12
HpaI GTT'AAC
Cuts at: 2320 3642 2320
Size: 1322 4730
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 2644 3651 2644
Size: 1007 5045
MluI A'CGCG_T
Cuts at: 5928 5928
Size: 6052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5166 5350 1305
Size: 3861 184 2007
Fragments arranged by size:
3861 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4230
Fragments arranged by size:
4230 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 2155 2157 3072 3099 4010 4369
Size: 648 855 134 2 915 27 911 359
Cuts at: 4369 4528 518
Size: 159 2042
Fragments arranged by size:
2042 915 911 855 648 359 159 134
27 2
MspA1I CmG'CkG
Cuts at: 1606 3331 3468 4145 5293 5538 1606
Size: 1725 137 677 1148 245 2120
Fragments arranged by size:
2120 1725 1148 677 245 137
NcoI C'CATG_G
Cuts at: 513 1065 2004 2670 513
Size: 552 939 666 3895
Fragments arranged by size:
3895 939 666 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6052
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6052
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 2284 3100 3410 4955 1132
Size: 97 1055 816 310 1545 2229
Fragments arranged by size:
2229 1545 1055 816 310 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6052
PciI A'CATG_T
Cuts at: 2280 3096 4951 2280
Size: 816 1855 3381
Fragments arranged by size:
3381 1855 816
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6052
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 5634 5666 4121
Size: 32 1481 32 4507
Fragments arranged by size:
4507 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 2700 2269
Size: 431 5621
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6052
PsiI TTA'TAA
Cuts at: 3622 3622
Size: 6052
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2267 2299 2267
Size: 32 6020
PstI C_TGCA'G
Cuts at: 830 2177 3404 830
Size: 1347 1227 3478
Fragments arranged by size:
3478 1347 1227
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
PvuII CAG'CTG
Cuts at: 3331 3331
Size: 6052
SalI G'TCGA_C
Cuts at: 1936 3394 1936
Size: 1458 4594
SanDI GG'GwC_CC
Cuts at: 2700 2700
Size: 6052
SbfI CC_TGCA'GG
Cuts at: 3404 3404
Size: 6052
ScaI AGT'ACT
Cuts at: 4300 6012 4300
Size: 1712 4340
SfcI C'TryA_G
Cuts at: 826 1045 2173 3001 3346 3400 4535 5216 5407
Size: 219 1128 828 345 54 1135 681 191
Cuts at: 5407 826
Size: 1471
Fragments arranged by size:
1471 1135 1128 828 681 345 219 191
54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6052
SmaI CCC'GGG
Cuts at: 1872 3379 1872
Size: 1507 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2207 2712 3131 4156 5057 5319
Size: 197 926 264 505 419 1025 901 262
Cuts at: 5319 820
Size: 1553
Fragments arranged by size:
1553 1025 926 901 505 419 264 262
197
SnaBI TAC'GTA
Cuts at: 493 3843 493
Size: 3350 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 3410 1132
Size: 2278 3774
SspI AAT'ATT
Cuts at: 5 52 3976 5
Size: 47 3924 2081
Fragments arranged by size:
3924 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6052
StyI C'CwwG_G
Cuts at: 513 1065 2004 2670 3189 3201 3491 513
Size: 552 939 666 519 12 290 3074
Fragments arranged by size:
3074 939 666 552 519 290 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2215 2492 4079 4096 4249 4434 4879
Size: 1255 635 277 1587 17 153 185 445
Cuts at: 4879 325
Size: 1498
Fragments arranged by size:
1587 1498 1255 635 445 277 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 4298 6010
Size: 274 80 33 51 1443 306 2015 1712
Cuts at: 6010 96
Size: 138
Fragments arranged by size:
2015 1712 1443 306 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2357 2389 6012 6044 2357
Size: 32 3623 32 2365
Fragments arranged by size:
3623 2365 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6052
XbaI T'CTAG_A
Cuts at: 3388 3388
Size: 6052
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6052
XmaI C'CCGG_G
Cuts at: 1870 3377 1870
Size: 1507 4545
XmnI GAAnn'nnTTC
Cuts at: 4181 4181
Size: 6052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3860 276
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI