Restriction map for
pFN21AB8291
(Circular) (Six-base) MAPSORT of: pFN21AB8291.seq Check: 3285 from: 1 to: 6121
pFN21AB8291 6121 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2445 2504 2445
Size: 59 6062
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2226 3360 3464 1937
Size: 289 1134 104 4594
Fragments arranged by size:
4594 1134 289 104
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2359 4184 5568 5836 1632
Size: 727 1825 1384 268 1917
Fragments arranged by size:
1917 1825 1384 727 268
AfeI AGC'GCT
Cuts at: 2670 2670
Size: 6121
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 5020 5997 1203
Size: 3817 977 1327
Fragments arranged by size:
3817 1327 977
AgeI A'CCGG_T
Cuts at: 2740 4952 2740
Size: 2212 3909
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 2168 1166
Size: 1002 5119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2186 3189 1184
Size: 1002 1003 4116
Fragments arranged by size:
4116 1003 1002
AlwNI CAG_nnn'CTG
Cuts at: 2156 2425 3077 3226 3864 5436 2156
Size: 269 652 149 638 1572 2841
Fragments arranged by size:
2841 1572 652 638 269 149
ApaI G_GGCC'C
Cuts at: 2553 2553
Size: 6121
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 2605 3430 3656 3808 5917 1493
Size: 1112 825 226 152 2109 1697
Fragments arranged by size:
2109 1697 1112 825 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3446 1870
Size: 73 1503 4545
Fragments arranged by size:
4545 1503 73
BamHI G'GATC_C
Cuts at: 3451 3818 3451
Size: 367 5754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2232 3173 3442 4897 618
Size: 325 217 627 445 941 269 1455 1842
Fragments arranged by size:
1842 1455 941 627 445 325 269 217
BanII G_rGCy'C
Cuts at: 1391 2294 2553 3440 1391
Size: 903 259 887 4072
Fragments arranged by size:
4072 903 887 259
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5150
BbvCI CC'TCA_GC
Cuts at: 2149 2665 2149
Size: 516 5605
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 1903
Size: 34 2375 34 3678
Fragments arranged by size:
3678 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3248 4732 136
Size: 107 122 71 631 605 1576 1484 1525
Fragments arranged by size:
1576 1525 1484 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6116 6116
Size: 6121
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 2175 2182 2553 3178 4182 5338 1790
Size: 385 7 371 625 1004 1156 2573
Fragments arranged by size:
2573 1156 1004 625 385 371 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2169 3852 4810
Size: 416 53 83 399 24 752 1683 958
Cuts at: 4810 442
Size: 1753
Fragments arranged by size:
1753 1683 958 752 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2462 2468 2657 3368 3437 4781 1239
Size: 986 237 6 189 711 69 1344 2579
Fragments arranged by size:
2579 1344 986 711 237 189 69 6
Bpu10I CC'TnA_GC
Cuts at: 2149 2531 2665 2149
Size: 382 134 5605
Fragments arranged by size:
5605 382 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4246 5111 5409 4246
Size: 865 298 4958
Fragments arranged by size:
4958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4784 882
Size: 617 3285 2219
Fragments arranged by size:
3285 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3912 493
Size: 711 2708 2702
Fragments arranged by size:
2708 2702 711
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3928 4310 4945 275
Size: 53 83 186 936 2395 382 635 1451
Fragments arranged by size:
2395 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2624 2740 4554 4952 5226 5373 5784 2624
Size: 116 1814 398 274 147 411 2961
Fragments arranged by size:
2961 1814 411 398 274 147 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2580 2585 2610 2822
Size: 30 165 30 1152 25 5 25 212
Cuts at: 2822 2852 3395 3425 1178
Size: 30 543 30 3874
Fragments arranged by size:
3874 1152 543 212 165 30 30 30
30 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2895 3344 3347 3350 3353 3356 3365 3368
Size: 1709 449 3 3 3 3 9 3
Cuts at: 3368 1186
Size: 3939
Fragments arranged by size:
3939 1709 449 9 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2023 2106 2199 2659 5324 5820 2023
Size: 83 93 460 2665 496 2324
Fragments arranged by size:
2665 2324 496 460 93 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3258 1163
Size: 573 1522 4026
Fragments arranged by size:
4026 1522 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 4182 4267 5338 2364
Size: 1818 85 1071 3147
Fragments arranged by size:
3147 1818 1071 85
BsmI GAATG_Cn'
Cuts at: 3632 3725 3632
Size: 93 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2182 2294 2364 2553 3178 3440
Size: 399 385 7 112 70 189 625 262
Cuts at: 3440 4182 4267 5338 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 625 399 385 262 189
112 85 70 7
BspEI T'CCGG_A
Cuts at: 2624 5784 2624
Size: 3160 2961
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2445 2504 2535 3225 3476 844
Size: 1601 59 31 690 251 3489
Fragments arranged by size:
3489 1601 690 251 59 31
BsrBI CCG'CTC
Cuts at: 1394 1823 2033 2248 4008 1394
Size: 429 210 215 1760 3507
Fragments arranged by size:
3507 1760 429 215 210
BsrDI GCAATG_nn'
Cuts at: 65 1248 4616 4790 65
Size: 1183 3368 174 1396
Fragments arranged by size:
3368 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2740 3111 4765 4952 1700
Size: 110 930 371 1654 187 2869
Fragments arranged by size:
2869 1654 930 371 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2938 3052 794
Size: 2144 114 3863
Fragments arranged by size:
3863 2144 114
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 2743 2752 2773 1184
Size: 1559 9 21 4532
Fragments arranged by size:
4532 1559 21 9
BstXI CCAn_nnnn'nTGG
Cuts at: 3208 3208
Size: 6121
BstYI r'GATC_y
Cuts at: 1972 2805 3073 3451 3818 4203 4220 5672 5758
Size: 833 268 378 367 385 17 1452 86
Cuts at: 5758 5770 6116 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 833 385 378 367 346 268
86 17 12
BstZ17I GTA'TAC
Cuts at: 2227 2227
Size: 6121
BtgI C'CryG_G
Cuts at: 513 1065 2748 2952 3246 513
Size: 552 1683 204 294 3388
Fragments arranged by size:
3388 1683 552 294 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2394 2671 3127 487
Size: 683 56 1168 277 456 3481
Fragments arranged by size:
3481 1168 683 456 277 56
BtsI GCAGTG_nn'
Cuts at: 3637 4431 4451 3637
Size: 794 20 5307
Fragments arranged by size:
5307 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 3249 3568
Size: 54 1425 50 275 18 216 1203 319
Cuts at: 3568 4457 6086 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1203 889 319 275 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2930 2960 3300 3321 4051 1353
Size: 1577 30 340 21 730 3423
Fragments arranged by size:
3423 1577 730 340 30 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2299 4708 5082 5228 387
Size: 1064 138 710 2409 374 146 1280
Fragments arranged by size:
2409 1280 1064 710 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2359 2462 2468 2657 3368 3437
Size: 393 593 134 103 6 189 711 69
Cuts at: 3437 4184 4781 5568 5836 1239
Size: 747 597 787 268 1524
Fragments arranged by size:
1524 787 747 711 597 593 393 268
189 134 103 69 6
EcoO109I rG'GnC_Cy
Cuts at: 2422 2549 2550 2593 2422
Size: 127 1 43 5950
Fragments arranged by size:
5950 127 43 1
EcoRI G'AATT_C
Cuts at: 2605 3430 3808 2605
Size: 825 378 4918
Fragments arranged by size:
4918 825 378
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 2672 5268 1678
Size: 994 2596 2531
Fragments arranged by size:
2596 2531 994
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2822 2849 2854 2881 3088
Size: 32 135 32 1250 27 5 27 207
Cuts at: 3088 3120 4762 4794 4836 4868 1373
Size: 32 1642 32 42 32 2626
Fragments arranged by size:
2626 1642 1250 207 135 42 32 32
32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2704 2824 3361 3465 3711 669
Size: 988 281 766 120 537 104 246 3079
Fragments arranged by size:
3079 988 766 537 281 246 120 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6121
HpaI GTT'AAC
Cuts at: 2824 3711 2824
Size: 887 5234
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5235 5419 1305
Size: 3930 184 2007
Fragments arranged by size:
3930 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 3251 10
Size: 54 1425 343 216 1203 2880
Fragments arranged by size:
2880 1425 1203 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2690 3251 4079 4438 4597 518
Size: 648 1002 522 561 828 359 159 2042
Fragments arranged by size:
2042 1002 828 648 561 522 359 159
MspA1I CmG'CkG
Cuts at: 1606 2153 2203 3537 4214 5362 5607 1606
Size: 547 50 1334 677 1148 245 2120
Fragments arranged by size:
2120 1334 1148 677 547 245 50
NcoI C'CATG_G
Cuts at: 513 1065 2748 3246 513
Size: 552 1683 498 3388
Fragments arranged by size:
3388 1683 552 498
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 2018 2900 3479 5024 1132
Size: 97 789 882 579 1545 2229
Fragments arranged by size:
2229 1545 882 789 579 97
PasI CC'CwG_GG
Cuts at: 1348 2287 2644 2761 2791 3179 1348
Size: 939 357 117 30 388 4290
Fragments arranged by size:
4290 939 388 357 117 30
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2440 1108
Size: 1332 4789
PfoI T'CCnGG_A
Cuts at: 2844 2844
Size: 6121
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2422 2593 2422
Size: 171 5950
PshAI GACnn'nnGTC
Cuts at: 1613 2699 3380 1613
Size: 1086 681 4354
Fragments arranged by size:
4354 1086 681
PsiI TTA'TAA
Cuts at: 3691 3691
Size: 6121
PspOMI G'GGCC_C
Cuts at: 2549 2549
Size: 6121
PstI C_TGCA'G
Cuts at: 830 2604 3048 3162 3222 3372 3473 830
Size: 1774 444 114 60 150 101 3478
Fragments arranged by size:
3478 1774 444 150 114 101 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 2153 2203 2153
Size: 50 6071
SalI G'TCGA_C
Cuts at: 1936 3359 3463 1936
Size: 1423 104 4594
Fragments arranged by size:
4594 1423 104
SapI GCTCTTCn'nnn_
Cuts at: 2930 2930
Size: 6121
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2600 3044 3158 3218 3368 3469 4604
Size: 219 1555 444 114 60 150 101 1135
Cuts at: 4604 5285 5476 826
Size: 681 191 1471
Fragments arranged by size:
1555 1471 1135 681 444 219 191 150
114 101 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4225 5126 5388 820
Size: 197 926 2282 901 262 1553
Fragments arranged by size:
2282 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 2900 3479 1132
Size: 1768 579 3774
Fragments arranged by size:
3774 1768 579
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6121
StyI C'CwwG_G
Cuts at: 513 1065 2118 2748 3246 3560 513
Size: 552 1053 630 498 314 3074
Fragments arranged by size:
3074 1053 630 552 498 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2300 2792 3134 4148 4165 4318 4503
Size: 1255 720 492 342 1014 17 153 185
Cuts at: 4503 4948 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1014 720 492 445 342 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4367 6079 96
Size: 274 80 33 51 1443 2390 1712 138
Fragments arranged by size:
2390 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2580 2612 3395 3427 6081 6113 2580
Size: 32 783 32 2654 32 2588
Fragments arranged by size:
2654 2588 783 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 2320
Size: 6121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 4250 4250
Size: 6121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI