(Circular) (Six-base) MAPSORT of: pFN21AB8288.seq Check: 6861 from: 1 to: 5926 pFN21AB8288 5926 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2297 3989 5373 5641 1632 Size: 665 1692 1384 268 1917 Fragments arranged by size: 1917 1692 1384 665 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 4825 5802 1203 Size: 3622 977 1327 Fragments arranged by size: 3622 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 3033 4655 3033 Size: 1622 4304 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2207 1184 Size: 1023 4903 AlwNI CAG_nnn'CTG Cuts at: 2035 2047 2337 2624 3669 5241 2035 Size: 12 290 287 1045 1572 2720 Fragments arranged by size: 2720 1572 1045 290 287 12 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 2099 3235 3461 3613 5722 1493 Size: 606 1136 226 152 2109 1697 Fragments arranged by size: 2109 1697 1136 606 226 152 AseI AT'TA_AT Cuts at: 160 1032 2448 4480 160 Size: 872 1416 2032 1606 Fragments arranged by size: 2032 1606 1416 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2971 3251 1870 Size: 73 1028 280 4545 Fragments arranged by size: 4545 1028 280 73 BamHI G'GATC_C Cuts at: 2284 2975 3256 3623 2284 Size: 691 281 367 4587 Fragments arranged by size: 4587 691 367 281 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2927 3247 4702 618 Size: 325 217 627 1140 320 1455 1842 Fragments arranged by size: 1842 1455 1140 627 325 320 217 BanII G_rGCy'C Cuts at: 1391 2191 2823 2843 3245 1391 Size: 800 632 20 402 4072 Fragments arranged by size: 4072 800 632 402 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2523 928 Size: 971 624 4331 Fragments arranged by size: 4331 971 624 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2917 2951 3022 3056 4117 4151 1903 Size: 34 980 34 71 34 1061 34 3678 Fragments arranged by size: 3678 1061 980 71 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 2550 1548 Size: 1002 4924 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2480 2645 4537 Size: 107 122 71 631 605 808 165 1892 Cuts at: 4537 136 Size: 1525 Fragments arranged by size: 1892 1525 808 631 605 165 122 107 71 BglII A'GATC_T Cuts at: 2667 5921 2667 Size: 3254 2672 BlpI GC'TnA_GC Cuts at: 2180 3343 2180 Size: 1163 4763 Bme1580I G_kGCm'C Cuts at: 1790 3987 5143 1790 Size: 2197 1156 2573 Fragments arranged by size: 2573 2197 1156 BmgBI CAC'GTC Cuts at: 1318 2584 2616 1318 Size: 1266 32 4628 Fragments arranged by size: 4628 1266 32 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3165 3657 4615 Size: 416 53 83 399 24 1748 492 958 Cuts at: 4615 442 Size: 1753 Fragments arranged by size: 1753 1748 958 492 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 2807 2837 3031 3082 3177 4586 1239 Size: 1283 285 30 194 51 95 1409 2579 Fragments arranged by size: 2579 1409 1283 285 194 95 51 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4051 4916 5214 4051 Size: 865 298 4763 Fragments arranged by size: 4763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4589 882 Size: 617 3090 2219 Fragments arranged by size: 3090 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3717 493 Size: 711 2513 2702 Fragments arranged by size: 2702 2513 711 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2345 2472 2633 3733 Size: 53 83 186 936 812 127 161 1100 Cuts at: 3733 4115 4750 275 Size: 382 635 1451 Fragments arranged by size: 1451 1100 936 812 635 382 186 161 127 83 53 BsaWI w'CCGG_w Cuts at: 2350 4359 4757 5031 5178 5589 2350 Size: 2009 398 274 147 411 2687 Fragments arranged by size: 2687 2009 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2839 2869 2924 2954 1178 Size: 30 165 30 1436 30 55 30 4150 Fragments arranged by size: 4150 1436 165 55 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2519 2662 1186 Size: 913 420 143 4450 Fragments arranged by size: 4450 913 420 143 BseYI C'CCAG_C Cuts at: 2959 2986 5129 5625 2959 Size: 27 2143 496 3260 Fragments arranged by size: 3260 2143 496 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5353 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3376 4137 5165 1540 Size: 275 186 1375 761 1028 2301 Fragments arranged by size: 2301 1375 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2922 3987 4072 5143 2922 Size: 1065 85 1071 3705 Fragments arranged by size: 3705 1071 1065 85 BsmI GAATG_Cn' Cuts at: 3437 3530 3437 Size: 93 5833 BsmBI CGTCTCn'nnnn_ Cuts at: 2352 2662 2352 Size: 310 5616 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2191 2823 2843 2922 3245 3987 4072 Size: 399 401 632 20 79 323 742 85 Cuts at: 4072 5143 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 632 401 399 323 85 79 20 BspEI T'CCGG_A Cuts at: 2350 5589 2350 Size: 3239 2687 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3281 844 Size: 2437 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2593 4570 4757 1700 Size: 110 783 1977 187 2869 Fragments arranged by size: 2869 1977 783 187 110 BsrGI T'GTAC_A Cuts at: 96 2247 96 Size: 2151 3775 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 2398 3986 4998 2398 Size: 1588 1012 3326 Fragments arranged by size: 3326 1588 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2047 794 Size: 1253 4673 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstXI CCAn_nnnn'nTGG Cuts at: 2596 2893 2596 Size: 297 5629 BstYI r'GATC_y Cuts at: 1972 2271 2284 2667 2975 3256 3623 4008 4025 Size: 299 13 383 308 281 367 385 17 Cuts at: 4025 5477 5563 5575 5921 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 383 367 346 308 299 281 86 17 13 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2529 513 Size: 552 939 525 3910 Fragments arranged by size: 3910 939 552 525 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2929 487 Size: 683 56 1703 3484 Fragments arranged by size: 3484 1703 683 56 BtsI GCAGTG_nn' Cuts at: 3038 3137 3442 4236 4256 3038 Size: 99 305 794 20 4708 Fragments arranged by size: 4708 794 305 99 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DraIII CAC_nnn'GTG Cuts at: 2025 2296 2025 Size: 271 5655 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2582 4933 809 Size: 507 1266 2351 1802 Fragments arranged by size: 2351 1802 1266 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2382 2661 Size: 54 1425 50 275 18 297 255 279 Cuts at: 2661 3148 3373 4262 5891 8 Size: 487 225 889 1629 43 Fragments arranged by size: 1629 1425 889 487 297 279 275 255 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2786 2868 3856 1353 Size: 1433 82 988 3423 Fragments arranged by size: 3423 1433 988 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2626 2698 4513 4887 5033 387 Size: 1064 138 1037 72 1815 374 146 1280 Fragments arranged by size: 1815 1280 1064 1037 374 146 138 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 2522 2807 2837 3031 3082 3177 Size: 393 665 225 285 30 194 51 95 Cuts at: 3177 3989 4586 5373 5641 1239 Size: 812 597 787 268 1524 Fragments arranged by size: 1524 812 787 665 597 393 285 268 225 194 95 51 30 EcoRI G'AATT_C Cuts at: 2099 3235 3613 2099 Size: 1136 378 4412 Fragments arranged by size: 4412 1136 378 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 2909 5073 1678 Size: 1231 2164 2531 Fragments arranged by size: 2531 2164 1231 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2272 2304 2839 2871 2924 Size: 32 135 32 700 32 535 32 53 Cuts at: 2924 2956 3019 3051 4567 4599 4641 4673 1373 Size: 32 63 32 1516 32 42 32 2626 Fragments arranged by size: 2626 1516 700 535 135 63 53 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5040 5224 1305 Size: 3735 184 2007 Fragments arranged by size: 3735 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2129 3150 10 Size: 54 1425 343 297 1021 2786 Fragments arranged by size: 2786 1425 1021 343 297 54 MslI CAynn'nnrTG Cuts at: 518 1166 3158 3884 4243 4402 518 Size: 648 1992 726 359 159 2042 Fragments arranged by size: 2042 1992 726 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2914 3088 3342 4019 5167 5412 1606 Size: 1308 174 254 677 1148 245 2120 Fragments arranged by size: 2120 1308 1148 677 254 245 174 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4435 Fragments arranged by size: 4435 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 3157 3284 4829 1132 Size: 97 1928 127 1545 2229 Fragments arranged by size: 2229 1928 1545 127 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5926 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 2136 1108 Size: 1028 4898 PfoI T'CCnGG_A Cuts at: 2265 2784 2265 Size: 519 5407 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PstI C_TGCA'G Cuts at: 830 2328 2460 3278 830 Size: 1498 132 818 3478 Fragments arranged by size: 3478 1498 818 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 3198 4174 5886 3198 Size: 976 1712 3238 Fragments arranged by size: 3238 1712 976 SfcI C'TryA_G Cuts at: 826 1045 2324 2456 3274 4409 5090 5281 826 Size: 219 1279 132 818 1135 681 191 1471 Fragments arranged by size: 1471 1279 1135 818 681 219 191 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 3253 1872 Size: 1381 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4030 4931 5193 820 Size: 197 926 2087 901 262 1553 Fragments arranged by size: 2087 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 3284 1132 Size: 2152 3774 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 2427 1517 Size: 910 5016 StyI C'CwwG_G Cuts at: 513 1065 2004 3365 513 Size: 552 939 1361 3074 Fragments arranged by size: 3074 1361 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2718 3953 3970 4123 4308 4753 325 Size: 1255 1138 1235 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1235 1138 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2247 2430 3196 Size: 274 80 33 51 1443 270 183 766 Cuts at: 3196 4172 5884 96 Size: 976 1712 138 Fragments arranged by size: 1712 1443 976 766 274 270 183 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5886 5918 5886 Size: 32 5894 Tth111I GACn'n_nGTC Cuts at: 1316 2851 1316 Size: 1535 4391 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 3251 1870 Size: 1381 4545 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI