Restriction map for
pFN21AB8286
(Circular) (Six-base) MAPSORT of: pFN21AB8286.seq Check: 7468 from: 1 to: 5902
pFN21AB8286 5902 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3245 1937
Size: 1308 4594
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 2488 4029 4402 1563
Size: 925 1541 373 3063
Fragments arranged by size:
3063 1541 925 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 2943 3965 5349 5617 1632
Size: 713 598 1022 1384 268 1917
Fragments arranged by size:
1917 1384 1022 713 598 268
AflII C'TTAA_G
Cuts at: 820 1017 2947 820
Size: 197 1930 3775
Fragments arranged by size:
3775 1930 197
AflIII A'CryG_T
Cuts at: 1203 2402 4801 5778 1203
Size: 1199 2399 977 1327
Fragments arranged by size:
2399 1327 1199 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 2323 2840 3645 5217 2323
Size: 517 805 1572 3008
Fragments arranged by size:
3008 1572 805 517
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 2651 2774 3211 3437 3589 5698 1493
Size: 1158 123 437 226 152 2109 1697
Fragments arranged by size:
2109 1697 1158 437 226 152 123
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2411 3051 3227 1870
Size: 73 468 640 176 4545
Fragments arranged by size:
4545 640 468 176 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2924 2957 2924
Size: 33 5869
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 3223 4678 618
Size: 325 217 627 281 1155 1455 1842
Fragments arranged by size:
1842 1455 1155 627 325 281 217
BanII G_rGCy'C
Cuts at: 1391 3221 1391
Size: 1830 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4093 4127 1903
Size: 34 50 34 2072 34 3678
Fragments arranged by size:
3678 2072 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 3963 5119 1790
Size: 2173 1156 2573
Fragments arranged by size:
2573 2173 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3633 4591 442
Size: 416 53 83 399 24 2216 958 1753
Fragments arranged by size:
2216 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2355 2373 1056
Size: 1299 18 4585
Fragments arranged by size:
4585 1299 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2307 4562 1239
Size: 1068 2255 2579
Fragments arranged by size:
2579 2255 1068
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3142 4027 4892 5190 3142
Size: 885 865 298 3854
Fragments arranged by size:
3854 885 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2405 3693 493
Size: 711 1201 1288 2702
Fragments arranged by size:
2702 1288 1201 711
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3709 4091 4726 275
Size: 53 83 186 936 2176 382 635 1451
Fragments arranged by size:
2176 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4335 4733 5007 5154 5565 4335
Size: 398 274 147 411 4672
Fragments arranged by size:
4672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2392 2422 1178
Size: 30 165 30 989 30 4658
Fragments arranged by size:
4658 989 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5902
BseYI C'CCAG_C
Cuts at: 5105 5601 5105
Size: 496 5406
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2750 3963 4048 5119 2750
Size: 1213 85 1071 3533
Fragments arranged by size:
3533 1213 1071 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2750 3221 3963 4048 5119 1391
Size: 399 960 471 742 85 1071 2174
Fragments arranged by size:
2174 1071 960 742 471 399 85
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3257 844
Size: 2413 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2666 4546 4733 1700
Size: 110 856 1880 187 2869
Fragments arranged by size:
2869 1880 856 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 2391 3962 4974 2391
Size: 1571 1012 3319
Fragments arranged by size:
3319 1571 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2434 3232 3599 3984 4001 5453 5539 5551
Size: 462 798 367 385 17 1452 86 12
Cuts at: 5551 5897 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 798 462 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2020 2020
Size: 5902
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 3418 4212 4232 3418
Size: 794 20 5088
Fragments arranged by size:
5088 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 2722 3081 3132 3206 2722
Size: 359 51 74 5418
Fragments arranged by size:
5418 359 74 51
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3349 4238 5867
Size: 54 1425 50 275 18 1519 889 1629
Cuts at: 5867 8
Size: 43
Fragments arranged by size:
1629 1519 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2922 3147 3832 1353
Size: 1569 225 685 3423
Fragments arranged by size:
3423 1569 685 225
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2345 2943 3965 4562 5349 5617
Size: 393 675 38 598 1022 597 787 268
Cuts at: 5617 1239
Size: 1524
Fragments arranged by size:
1524 1022 787 675 598 597 393 268
38
EcoO109I rG'GnC_Cy
Cuts at: 2224 2320 2224
Size: 96 5806
EcoRI G'AATT_C
Cuts at: 2774 3211 3589 2774
Size: 437 378 5087
Fragments arranged by size:
5087 437 378
EcoRV GAT'ATC
Cuts at: 2387 2387
Size: 5902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2962 2962
Size: 5902
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 5049 1678
Size: 3371 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2815 2829 2847 2861 4543
Size: 32 135 32 1243 14 18 14 1682
Cuts at: 4543 4575 4617 4649 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1682 1243 135 42 32 32 32
32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2595 2787 3246 3492 669
Size: 988 281 657 192 459 246 3079
Fragments arranged by size:
3079 988 657 459 281 246 192
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2102 2542 5016 5200 1305
Size: 797 440 2474 184 2007
Fragments arranged by size:
2474 2007 797 440 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4080
Fragments arranged by size:
4080 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 4219 4378 518
Size: 648 2694 359 159 2042
Fragments arranged by size:
2694 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3318 3995 5143 5388 1606
Size: 1712 677 1148 245 2120
Fragments arranged by size:
2120 1712 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5350
NdeI CA'TA_TG
Cuts at: 387 2088 387
Size: 1701 4201
NheI G'CTAG_C
Cuts at: 1052 2351 2369 1052
Size: 1299 18 4585
Fragments arranged by size:
4585 1299 18
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 3260 4805 1132
Size: 97 2031 1545 2229
Fragments arranged by size:
2229 2031 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5902
PfoI T'CCnGG_A
Cuts at: 2833 2833
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2224 2320 2224
Size: 96 5806
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PstI C_TGCA'G
Cuts at: 830 3254 830
Size: 2424 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 3076 3127 4150 5862 3076
Size: 51 1023 1712 3116
Fragments arranged by size:
3116 1712 1023 51
SfcI C'TryA_G
Cuts at: 826 1045 3250 4385 5066 5257 826
Size: 219 2205 1135 681 191 1471
Fragments arranged by size:
2205 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2947 3121 4006 4907 5169 820
Size: 197 926 1004 174 885 901 262 1553
Fragments arranged by size:
1553 1004 926 901 885 262 197 174
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 2479 3826 5
Size: 47 2427 1347 2081
Fragments arranged by size:
2427 2081 1347 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5902
StyI C'CwwG_G
Cuts at: 513 1065 3341 513
Size: 552 2276 3074
Fragments arranged by size:
3074 2276 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2499 3929 3946 4099 4284 4729 325
Size: 1255 919 1430 17 153 185 445 1498
Fragments arranged by size:
1498 1430 1255 919 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2918 3074 3125
Size: 274 80 33 51 1443 941 156 51
Cuts at: 3125 4148 5860 96
Size: 1023 1712 138
Fragments arranged by size:
1712 1443 1023 941 274 156 138 80
51 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2079 2079
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 2330 4031 2330
Size: 1701 4201
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI