Restriction map for
pFN21AB8284
(Circular) (Six-base) MAPSORT of: pFN21AB8284.seq Check: 3806 from: 1 to: 5941
pFN21AB8284 5941 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3177 3284 1937
Size: 1240 107 4594
Fragments arranged by size:
4594 1240 107
Acc65I G'GTAC_C
Cuts at: 2561 3262 2561
Size: 701 5240
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2249 2450 2522 2979 3224 4004 5388 5656
Size: 617 201 72 457 245 780 1384 268
Cuts at: 5656 1632
Size: 1917
Fragments arranged by size:
1917 1384 780 617 457 268 245 201
72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 2100 2447 4840 5817 1203
Size: 897 347 2393 977 1327
Fragments arranged by size:
2393 1327 977 897 347
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 2503 4670 2503
Size: 2167 3774
AleI CACnn'nnGTG
Cuts at: 1166 2694 3044 1166
Size: 1528 350 4063
Fragments arranged by size:
4063 1528 350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AlwNI CAG_nnn'CTG
Cuts at: 2473 3002 3684 5256 2473
Size: 529 682 1572 3158
Fragments arranged by size:
3158 1572 682 529
ApaI G_GGCC'C
Cuts at: 2945 2945
Size: 5941
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 3250 3476 3628 5737 1493
Size: 1757 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2454 2493 2692 3266 1870
Size: 73 511 39 199 574 4545
Fragments arranged by size:
4545 574 511 199 73 39
BamHI G'GATC_C
Cuts at: 2013 3271 3638 2013
Size: 1258 367 4316
Fragments arranged by size:
4316 1258 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2561 3262 4717 618
Size: 325 217 627 774 701 1455 1842
Fragments arranged by size:
1842 1455 774 701 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2945 3260 1391
Size: 1554 315 4072
Fragments arranged by size:
4072 1554 315
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2499 928
Size: 971 600 4370
Fragments arranged by size:
4370 971 600
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3045 3833 5043 3045
Size: 788 1210 3943
Fragments arranged by size:
3943 1210 788
BclI T'GATC_A
Cuts at: 1548 2338 2922 1548
Size: 790 584 4567
Fragments arranged by size:
4567 790 584
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2176 4552 136
Size: 107 122 71 631 605 504 2376 1525
Fragments arranged by size:
2376 1525 631 605 504 122 107 71
BglII A'GATC_T
Cuts at: 3114 5936 3114
Size: 2822 3119
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2455 2945 4002 5158 1790
Size: 665 490 1057 1156 2573
Fragments arranged by size:
2573 1156 1057 665 490
BmgBI CAC'GTC
Cuts at: 1318 2198 1318
Size: 880 5061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 4630 442
Size: 416 53 83 399 24 2255 958 1753
Fragments arranged by size:
2255 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2551 3041 4601 1239
Size: 845 467 490 1560 2579
Fragments arranged by size:
2579 1560 845 490 467
Bpu10I CC'TnA_GC
Cuts at: 2434 2434
Size: 5941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4066 4931 5229 4066
Size: 865 298 4778
Fragments arranged by size:
4778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2067 2150 2545 4604 882
Size: 617 568 83 395 2059 2219
Fragments arranged by size:
2219 2059 617 568 395 83
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3748 4130 4765 275
Size: 53 83 186 936 2215 382 635 1451
Fragments arranged by size:
2215 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2202 2874 4374 4772 5046 5193 5604 2202
Size: 672 1500 398 274 147 411 2539
Fragments arranged by size:
2539 1500 672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5716
Fragments arranged by size:
5716 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2757 1186
Size: 1571 4370
BseYI C'CCAG_C
Cuts at: 2018 2223 2584 2618 5144 5640 2018
Size: 205 361 34 2526 496 2319
Fragments arranged by size:
2526 2319 496 361 205 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5368
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 4002 4087 5158 2070
Size: 1932 85 1071 2853
Fragments arranged by size:
2853 1932 1071 85
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
BsmBI CGTCTCn'nnnn_
Cuts at: 2188 2188
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2455 2945 3260 4002 4087 5158
Size: 399 280 385 490 315 742 85 1071
Cuts at: 5158 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 490 399 385 315 280
85
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3099 3830 5560 3099
Size: 731 1730 3480
Fragments arranged by size:
3480 1730 731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2796 3018 3296 844
Size: 1952 222 278 3489
Fragments arranged by size:
3489 1952 278 222
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2709 3828 1394
Size: 429 188 698 1119 3507
Fragments arranged by size:
3507 1119 698 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 2358 4436 4610 65
Size: 1183 1110 2078 174 1396
Fragments arranged by size:
2078 1396 1183 1110 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2175 4585 4772 1700
Size: 110 365 2410 187 2869
Fragments arranged by size:
2869 2410 365 187 110
BsrGI T'GTAC_A
Cuts at: 96 2112 96
Size: 2016 3925
BssHII G'CGCG_C
Cuts at: 1925 2022 1925
Size: 97 5844
BssSI C'ACGA_G
Cuts at: 2483 4001 5013 2483
Size: 1518 1012 3411
Fragments arranged by size:
3411 1518 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstXI CCAn_nnnn'nTGG
Cuts at: 2599 2599
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 2013 2233 3114 3271 3638 4023 4040 5492
Size: 41 220 881 157 367 385 17 1452
Cuts at: 5492 5578 5590 5936 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 881 385 367 346 220 157
86 41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2797 513
Size: 552 939 793 3657
Fragments arranged by size:
3657 939 793 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2203 487
Size: 683 56 977 4225
Fragments arranged by size:
4225 977 683 56
BtsI GCAGTG_nn'
Cuts at: 2292 2776 2920 3457 4251 4271 2292
Size: 484 144 537 794 20 3962
Fragments arranged by size:
3962 794 537 484 144 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3222 3245 3222
Size: 23 5918
DraIII CAC_nnn'GTG
Cuts at: 2536 2536
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3185 4948 809
Size: 507 1869 1763 1802
Fragments arranged by size:
1869 1802 1763 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2475 2628 3388
Size: 54 1425 50 275 18 645 153 760
Cuts at: 3388 4277 5906 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 760 645 275 153 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3187 3871 1353
Size: 1834 684 3423
Fragments arranged by size:
3423 1834 684
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2249 2450 2522 2551 2979 3041
Size: 393 452 165 201 72 29 428 62
Cuts at: 3041 3224 4004 4601 5388 5656 1239
Size: 183 780 597 787 268 1524
Fragments arranged by size:
1524 787 780 597 452 428 393 268
201 183 165 72 62 29
EcoO109I rG'GnC_Cy
Cuts at: 2497 2645 2497
Size: 148 5793
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 5941
FspI TGC'GCA
Cuts at: 2768 4447 2768
Size: 1679 4262
HaeII r_GCGC'y
Cuts at: 1678 5088 1678
Size: 3410 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2489 2521 2671 2703 2811
Size: 32 135 32 917 32 150 32 108
Cuts at: 2811 2843 3101 3106 3133 3138 4582 4614 4656
Size: 32 258 5 27 5 1444 32 42
Cuts at: 4656 4688 1373
Size: 32 2626
Fragments arranged by size:
2626 1444 917 258 150 135 108 42
32 32 32 32 32 32 32 27
5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3178 3285 3531 669
Size: 988 281 1240 107 246 3079
Fragments arranged by size:
3079 1240 988 281 246 107
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 2565 3266 2565
Size: 701 5240
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5055 5239 1305
Size: 3750 184 2007
Fragments arranged by size:
3750 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2477 10
Size: 54 1425 343 645 3474
Fragments arranged by size:
3474 1425 645 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2694 3044 3899 4258 4417 518
Size: 648 1528 350 855 359 159 2042
Fragments arranged by size:
2042 1528 855 648 359 350 159
MspA1I CmG'CkG
Cuts at: 1606 2227 2677 3357 4034 5182 5427 1606
Size: 621 450 680 677 1148 245 2120
Fragments arranged by size:
2120 1148 680 677 621 450 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4450
Fragments arranged by size:
4450 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 2104 2315 2451 3299 4844 1132
Size: 97 875 211 136 848 1545 2229
Fragments arranged by size:
2229 1545 875 848 211 136 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 2100 2447 4840 2100
Size: 347 2393 3201
Fragments arranged by size:
3201 2393 347
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2536 1108
Size: 1428 4513
PfoI T'CCnGG_A
Cuts at: 2567 2567
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2497 2645 2497
Size: 148 5793
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2941 2941
Size: 5941
PspXI vC'TCGA_Gb
Cuts at: 2692 2692
Size: 5941
PstI C_TGCA'G
Cuts at: 830 2370 2556 3293 830
Size: 1540 186 737 3478
Fragments arranged by size:
3478 1540 737 186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2227 2227
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3176 3283 1936
Size: 1240 107 4594
Fragments arranged by size:
4594 1240 107
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 2366 2552 3289 4424 5105 5296 826
Size: 219 1321 186 737 1135 681 191 1471
Fragments arranged by size:
1471 1321 1135 737 681 219 191 186
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2456 3268 1872
Size: 584 812 4545
Fragments arranged by size:
4545 812 584
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2692 4045 4946 5208 820
Size: 197 926 749 1353 901 262 1553
Fragments arranged by size:
1553 1353 926 901 749 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 3066 1517
Size: 1549 4392
StyI C'CwwG_G
Cuts at: 513 1065 2004 3380 513
Size: 552 939 1376 3074
Fragments arranged by size:
3074 1376 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2537 2964 3968 3985 4138 4323 4768
Size: 1255 957 427 1004 17 153 185 445
Cuts at: 4768 325
Size: 1498
Fragments arranged by size:
1498 1255 1004 957 445 427 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 4187 5899
Size: 274 80 33 51 1443 135 2075 1712
Cuts at: 5899 96
Size: 138
Fragments arranged by size:
2075 1712 1443 274 138 135 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 2692 1943
Size: 749 5192
XmaI C'CCGG_G
Cuts at: 1870 2454 3266 1870
Size: 584 812 4545
Fragments arranged by size:
4545 812 584
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI