Restriction map for
pFN21AB8281
(Circular) (Six-base) MAPSORT of: pFN21AB8281.seq Check: 661 from: 1 to: 5620
pFN21AB8281 5620 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2684 3683 5067 5335 1632
Size: 1052 999 1384 268 1917
Fragments arranged by size:
1917 1384 1052 999 268
AfeI AGC'GCT
Cuts at: 2206 2206
Size: 5620
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 2489 2714 1166
Size: 1323 225 4072
Fragments arranged by size:
4072 1323 225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AlwNI CAG_nnn'CTG
Cuts at: 3363 4935 3363
Size: 1572 4048
ApaLI G'TGCA_C
Cuts at: 2492 3677 4833 2492
Size: 1185 1156 3279
Fragments arranged by size:
3279 1185 1156
ApoI r'AATT_y
Cuts at: 1493 2929 3155 3307 5416 1493
Size: 1436 226 152 2109 1697
Fragments arranged by size:
2109 1697 1436 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2833 2872 2916 2945 1870
Size: 73 890 39 44 29 4545
Fragments arranged by size:
4545 890 73 44 39 29
AvrII C'CTAG_G
Cuts at: 2732 2732
Size: 5620
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2080 2471 2939 1391
Size: 689 391 468 4072
Fragments arranged by size:
4072 689 468 391
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4649
BbvCI CC'TCA_GC
Cuts at: 2305 2305
Size: 5620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BfrBI ATG'CAT
Cuts at: 2596 2596
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2657 2720 2791
Size: 107 122 71 631 605 985 63 71
Cuts at: 2791 4231 136
Size: 1440 1525
Fragments arranged by size:
1525 1440 985 631 605 122 107 71
71 63
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 2496 3681 4837 1790
Size: 706 1185 1156 2573
Fragments arranged by size:
2573 1185 1156 706
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2490 2490
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2248 2278 2314 2332 2884 4280 1239
Size: 1009 30 36 18 552 1396 2579
Fragments arranged by size:
2579 1396 1009 552 36 30 18
Bpu10I CC'TnA_GC
Cuts at: 2257 2305 2320 2478 2257
Size: 48 15 158 5399
Fragments arranged by size:
5399 158 48 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2266 3745 4610 4908 2266
Size: 1479 865 298 2978
Fragments arranged by size:
2978 1479 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2238 2805 4283 882
Size: 617 739 567 1478 2219
Fragments arranged by size:
2219 1478 739 617 567
BsaAI yAC'GTr
Cuts at: 493 1204 2626 3411 493
Size: 711 1422 785 2702
Fragments arranged by size:
2702 1422 785 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2062 2092 1178
Size: 30 165 30 659 30 4706
Fragments arranged by size:
4706 659 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2830 2902 1186
Size: 1644 72 3904
Fragments arranged by size:
3904 1644 72
BseYI C'CCAG_C
Cuts at: 4823 5319 4823
Size: 496 5124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2179 3070 3831 4859 1540
Size: 275 186 178 891 761 1028 2301
Fragments arranged by size:
2301 1028 891 761 275 186 178
BsiHKAI G_wGCw'C
Cuts at: 2496 3681 3766 4837 2496
Size: 1185 85 1071 3279
Fragments arranged by size:
3279 1185 1071 85
BsiWI C'GTAC_G
Cuts at: 2622 2622
Size: 5620
BsmI GAATG_Cn'
Cuts at: 3131 3224 3131
Size: 93 5527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2471 2496 2939 3681 3766 4837
Size: 399 290 391 25 443 742 85 1071
Cuts at: 4837 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 443 399 391 290 85
25
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2787 2832 2975 844
Size: 1943 45 143 3489
Fragments arranged by size:
3489 1943 143 45
BsrBI CCG'CTC
Cuts at: 1394 1823 2106 2319 2418 2829 2898 3507 1394
Size: 429 283 213 99 411 69 609 3507
Fragments arranged by size:
3507 609 429 411 283 213 99 69
BsrDI GCAATG_nn'
Cuts at: 65 1248 4115 4289 65
Size: 1183 2867 174 1396
Fragments arranged by size:
2867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2950 3317 3702 3719 5171 5257 5269 5615
Size: 978 367 385 17 1452 86 12 346
Cuts at: 5615 1972
Size: 1977
Fragments arranged by size:
1977 1452 978 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2583 2807 513
Size: 552 939 579 224 3326
Fragments arranged by size:
3326 939 579 552 224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 2293 2703 3136 3930 3950 2293
Size: 410 433 794 20 3963
Fragments arranged by size:
3963 794 433 410 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2176 2692 3067
Size: 54 1425 50 275 18 346 516 375
Cuts at: 3067 3956 5585 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 516 375 346 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2176 3067 1537
Size: 275 364 891 4090
Fragments arranged by size:
4090 891 364 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2305 2368 3550 1353
Size: 671 281 63 1182 3423
Fragments arranged by size:
3423 1182 671 281 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2479 4207 4581 4727 387
Size: 1064 138 890 1728 374 146 1280
Fragments arranged by size:
1728 1280 1064 890 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2248 2278 2314 2332 2684 2884 3683
Size: 393 616 30 36 18 352 200 799
Cuts at: 3683 4280 5067 5335 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 799 787 616 597 393 352 268
200 36 30 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2731 2731
Size: 5620
EcoO109I rG'GnC_Cy
Cuts at: 2823 2844 2823
Size: 21 5599
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 5620
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 2208 2413 2663 4767 1678
Size: 530 205 250 2104 2531
Fragments arranged by size:
2531 2104 530 250 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2512 2544 4261
Size: 32 135 32 530 32 378 32 1717
Cuts at: 4261 4293 4335 4367 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1717 530 378 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2931 4734 4918 1305
Size: 1626 1803 184 2007
Fragments arranged by size:
2007 1803 1626 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2694 10
Size: 54 1425 343 862 2936
Fragments arranged by size:
2936 1425 862 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 2582 2673 2714 3578 3937 4096
Size: 648 1323 93 91 41 864 359 159
Cuts at: 4096 518
Size: 2042
Fragments arranged by size:
2042 1323 864 648 359 159 93 91
41
MspA1I CmG'CkG
Cuts at: 1606 3036 3713 4861 5106 1606
Size: 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4129
Fragments arranged by size:
4129 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NsiI A_TGCA'T
Cuts at: 2598 2598
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2581 2978 4523 1132
Size: 97 1352 397 1545 2229
Fragments arranged by size:
2229 1545 1352 397 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5620
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2823 2844 2823
Size: 21 5599
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2972 830
Size: 2142 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SapI GCTCTTCn'nnn_
Cuts at: 2024 2305 2368 2024
Size: 281 63 5276
Fragments arranged by size:
5276 281 63
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SexAI A'CCwGG_T
Cuts at: 2228 2228
Size: 5620
SfcI C'TryA_G
Cuts at: 826 1045 2968 4103 4784 4975 826
Size: 219 1923 1135 681 191 1471
Fragments arranged by size:
1923 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2874 2947 1872
Size: 1002 73 4545
Fragments arranged by size:
4545 1002 73
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2281 3724 4625 4887 820
Size: 197 926 338 1443 901 262 1553
Fragments arranged by size:
1553 1443 926 901 338 262 197
SnaBI TAC'GTA
Cuts at: 493 2626 3411 493
Size: 2133 785 2702
Fragments arranged by size:
2702 2133 785
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2151 2732 3059 513
Size: 552 939 117 30 581 327 3074
Fragments arranged by size:
3074 939 581 552 327 117 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2226 3647 3664 3817 4002 4447 325
Size: 1255 646 1421 17 153 185 445 1498
Fragments arranged by size:
1498 1421 1255 646 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3866 5578 96
Size: 274 80 33 51 1443 1889 1712 138
Fragments arranged by size:
1889 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2062 2094 5580 5612 2062
Size: 32 3486 32 2070
Fragments arranged by size:
3486 2070 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2716 2787 2716
Size: 71 5549
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2872 2945 1870
Size: 1002 73 4545
Fragments arranged by size:
4545 1002 73
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI