Restriction map for
pFN21AB8271
(Circular) (Six-base) MAPSORT of: pFN21AB8271.seq Check: 3074 from: 1 to: 5656
pFN21AB8271 5656 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2185 2487 2752 2185
Size: 302 265 5089
Fragments arranged by size:
5089 302 265
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3719 5103 5371 1632
Size: 2087 1384 268 1917
Fragments arranged by size:
2087 1917 1384 268
AfeI AGC'GCT
Cuts at: 2132 2132
Size: 5656
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 2388 4555 5532 1203
Size: 1185 2167 977 1327
Fragments arranged by size:
2167 1327 1185 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 2185 2236 3399 4971 2185
Size: 51 1163 1572 2870
Fragments arranged by size:
2870 1572 1163 51
ApaI G_GGCC'C
Cuts at: 2557 2607 2680 2557
Size: 50 73 5533
Fragments arranged by size:
5533 73 50
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2965 3191 3343 5452 1493
Size: 1472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2093 2981 1870
Size: 73 150 888 4545
Fragments arranged by size:
4545 888 150 73
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2564 2921 2977 4432 618
Size: 325 217 627 777 357 56 1455 1842
Fragments arranged by size:
1842 1455 777 627 357 325 217 56
BanII G_rGCy'C
Cuts at: 1391 2094 2557 2607 2648 2680 2859 2975 1391
Size: 703 463 50 41 32 179 116 4072
Fragments arranged by size:
4072 703 463 179 116 50 41 32
BbeI G_GCGC'C
Cuts at: 2568 2568
Size: 5656
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2874 928
Size: 971 975 3710
Fragments arranged by size:
3710 975 971
BbvCI CC'TCA_GC
Cuts at: 2579 2579
Size: 5656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2831 4267 136
Size: 107 122 71 631 605 1159 1436 1525
Fragments arranged by size:
1525 1436 1159 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 2213 3073 2213
Size: 860 4796
Bme1580I G_kGCm'C
Cuts at: 1790 2332 2557 2607 2680 2924 2943 3717 4873
Size: 542 225 50 73 244 19 774 1156
Cuts at: 4873 1790
Size: 2573
Fragments arranged by size:
2573 1156 774 542 244 225 73 50
19
BmgBI CAC'GTC
Cuts at: 1318 2389 1318
Size: 1071 4585
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2156 3387 4345
Size: 416 53 83 399 24 739 1231 958
Cuts at: 4345 442
Size: 1753
Fragments arranged by size:
1753 1231 958 739 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2807 4316 1239
Size: 1073 495 1509 2579
Fragments arranged by size:
2579 1509 1073 495
Bpu10I CC'TnA_GC
Cuts at: 2579 2579
Size: 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2825 3781 4646 4944 2825
Size: 956 865 298 3537
Fragments arranged by size:
3537 956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2249 4319 882
Size: 617 750 2070 2219
Fragments arranged by size:
2219 2070 750 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2565 3463 3845 4480
Size: 53 83 186 936 1032 898 382 635
Cuts at: 4480 275
Size: 1451
Fragments arranged by size:
1451 1032 936 898 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2375 2405 1178
Size: 30 165 30 972 30 4429
Fragments arranged by size:
4429 972 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2333 2465 2492 2729 2804 1186
Size: 1033 114 132 27 237 75 4038
Fragments arranged by size:
4038 1033 237 132 114 75 27
BseYI C'CCAG_C
Cuts at: 2133 2172 2368 2799 2942 4859 5355 2133
Size: 39 196 431 143 1917 496 2434
Fragments arranged by size:
2434 1917 496 431 196 143 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2219 2426 2444 2927 1163
Size: 573 462 21 207 18 483 3892
Fragments arranged by size:
3892 573 483 462 207 21 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2398 2740 3106 3867 4895 1540
Size: 275 186 397 342 366 761 1028 2301
Fragments arranged by size:
2301 1028 761 397 366 342 275 186
BsiHKAI G_wGCw'C
Cuts at: 2447 3717 3802 4873 2447
Size: 1270 85 1071 3230
Fragments arranged by size:
3230 1270 1071 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
BsmBI CGTCTCn'nnnn_
Cuts at: 2315 2315
Size: 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2332 2447 2557 2607 2648 2680
Size: 399 304 238 115 110 50 41 32
Cuts at: 2680 2859 2924 2943 2975 3717 3802 4873 1391
Size: 179 65 19 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 304 238 179 115
110 85 65 50 41 32 32 19
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 2185 2487 2529 2752 3011 844
Size: 1331 10 302 42 223 259 3489
Fragments arranged by size:
3489 1331 302 259 223 42 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2124 2674 2695 3543 1394
Size: 429 301 550 21 848 3507
Fragments arranged by size:
3507 848 550 429 301 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2654 4300 4487 1700
Size: 110 844 1646 187 2869
Fragments arranged by size:
2869 1646 844 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 2701 2703 2776 1925
Size: 776 2 73 4805
Fragments arranged by size:
4805 776 73 2
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2431 794
Size: 1637 4019
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 2224 1184
Size: 1040 4616
BstYI r'GATC_y
Cuts at: 1972 2986 3353 3738 3755 5207 5293 5305 5651
Size: 1014 367 385 17 1452 86 12 346
Cuts at: 5651 1972
Size: 1977
Fragments arranged by size:
1977 1452 1014 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2413 2916 513
Size: 552 1348 503 3253
Fragments arranged by size:
3253 1348 552 503
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2523 487
Size: 683 56 1297 3620
Fragments arranged by size:
3620 1297 683 56
BtsI GCAGTG_nn'
Cuts at: 2544 3172 3966 3986 2544
Size: 628 794 20 4214
Fragments arranged by size:
4214 794 628 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 2395 2437
Size: 54 1425 50 275 18 295 270 42
Cuts at: 2437 2652 2656 3103 3992 5621 8
Size: 215 4 447 889 1629 43
Fragments arranged by size:
1629 1425 889 447 295 275 270 215
54 50 43 42 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2395 3103 1537
Size: 275 583 708 4090
Fragments arranged by size:
4090 708 583 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3586 1353
Size: 2233 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2330 2783 4243 4617 4763 387
Size: 1064 138 741 453 1460 374 146 1280
Fragments arranged by size:
1460 1280 1064 741 453 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2807 3719 4316 5103 5371 1239
Size: 393 680 495 912 597 787 268 1524
Fragments arranged by size:
1524 912 787 680 597 495 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2747 2747
Size: 5656
EcoO109I rG'GnC_Cy
Cuts at: 2553 2603 2604 2553
Size: 50 1 5605
Fragments arranged by size:
5605 50 1
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FseI GG_CCGG'CC
Cuts at: 2658 2658
Size: 5656
FspI TGC'GCA
Cuts at: 2483 4162 2483
Size: 1679 3977
HaeII r_GCGC'y
Cuts at: 1678 2134 2246 2533 2568 2586 2599 2881 2931
Size: 456 112 287 35 18 13 282 50
Cuts at: 2931 4803 1678
Size: 1872 2531
Fragments arranged by size:
2531 1872 456 287 282 112 50 35
18 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373
Size: 32 135 32 2725 32 42 32 2626
Fragments arranged by size:
2725 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KasI G'GCGC_C
Cuts at: 2564 2564
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3834
Fragments arranged by size:
3834 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3614 3973 4132 518
Size: 648 2448 359 159 2042
Fragments arranged by size:
2448 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2415 3072 3749 4897 5142 1606
Size: 809 657 677 1148 245 2120
Fragments arranged by size:
2120 1148 809 677 657 245
NaeI GCC'GGC
Cuts at: 2656 2656
Size: 5656
NarI GG'CG_CC
Cuts at: 2565 2565
Size: 5656
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5104
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NgoMIV G'CCGG_C
Cuts at: 2654 2654
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 2395 3103 2395
Size: 708 4948
NspI r_CATG'y
Cuts at: 1132 1229 2875 3014 4559 1132
Size: 97 1646 139 1545 2229
Fragments arranged by size:
2229 1646 1545 139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PspOMI G'GGCC_C
Cuts at: 2553 2603 2676 2553
Size: 50 73 5533
Fragments arranged by size:
5533 73 50
PstI C_TGCA'G
Cuts at: 830 2352 2526 2817 3008 830
Size: 1522 174 291 191 3478
Fragments arranged by size:
3478 1522 291 191 174
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
SacII CC_GC'GG
Cuts at: 2416 2416
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 2817 3008 2817
Size: 191 5465
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2348 2522 2666 2813 3004 4139 4820
Size: 219 1303 174 144 147 191 1135 681
Cuts at: 4820 5011 826
Size: 191 1471
Fragments arranged by size:
1471 1303 1135 681 219 191 191 174
147 144
SfiI GGCCn_nnn'nGGCC
Cuts at: 2831 2831
Size: 5656
SfoI GGC'GCC
Cuts at: 2566 2566
Size: 5656
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2840 3760 4661 4923 820
Size: 197 926 897 920 901 262 1553
Fragments arranged by size:
1553 926 920 901 897 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 2276 2684 2825 1517
Size: 759 408 141 4348
Fragments arranged by size:
4348 759 408 141
StyI C'CwwG_G
Cuts at: 513 1065 2058 2340 3095 513
Size: 552 993 282 755 3074
Fragments arranged by size:
3074 993 755 552 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2126 2164 2243 3683 3700 3853 4038
Size: 1255 546 38 79 1440 17 153 185
Cuts at: 4038 4483 325
Size: 445 1498
Fragments arranged by size:
1498 1440 1255 546 445 185 153 79
38 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2375 2407 5616 5648 2375
Size: 32 3209 32 2383
Fragments arranged by size:
3209 2383 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2185 1316
Size: 869 4787
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacII SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI