Restriction map for
pFN21AB8270
(Circular) (Six-base) MAPSORT of: pFN21AB8270.seq Check: 967 from: 1 to: 5512
pFN21AB8270 5512 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2132 2132
Size: 5512
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2718 3322 278
Size: 53 83 186 936 1182 604 2468
Fragments arranged by size:
2468 1182 936 604 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2855 1937
Size: 918 4594
Acc65I G'GTAC_C
Cuts at: 2833 2833
Size: 5512
AclI AA'CG_TT
Cuts at: 1563 3639 4012 1563
Size: 2076 373 3063
Fragments arranged by size:
3063 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 2210 2771 3575 4959 5227 1632
Size: 560 18 561 804 1384 268 1917
Fragments arranged by size:
1917 1384 804 561 560 268 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5315
AflIII A'CryG_T
Cuts at: 1203 4411 5388 1203
Size: 3208 977 1327
Fragments arranged by size:
3208 1327 977
AgeI A'CCGG_T
Cuts at: 2553 4343 2553
Size: 1790 3722
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5512
AlwNI CAG_nnn'CTG
Cuts at: 3255 4827 3255
Size: 1572 3940
ApaLI G'TGCA_C
Cuts at: 3569 4725 3569
Size: 1156 4356
ApoI r'AATT_y
Cuts at: 1493 2821 3047 3199 5308 1493
Size: 1328 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2276 2708 2837 1870
Size: 73 333 432 129 4545
Fragments arranged by size:
4545 432 333 129 73
BamHI G'GATC_C
Cuts at: 2842 3209 2842
Size: 367 5145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2160 2564 2833 4288
Size: 325 217 627 261 112 404 269 1455
Cuts at: 4288 618
Size: 1842
Fragments arranged by size:
1842 1455 627 404 325 269 261 217
112
BanII G_rGCy'C
Cuts at: 1391 2283 2582 2831 1391
Size: 892 299 249 4072
Fragments arranged by size:
4072 892 299 249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2074 2103 2260 928
Size: 971 175 29 157 4180
Fragments arranged by size:
4180 971 175 157 29
BbvCI CC'TCA_GC
Cuts at: 2655 2655
Size: 5512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2461 2495 3703 3737 1903
Size: 34 524 34 1208 34 3678
Fragments arranged by size:
3678 1208 524 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4614 3404
Size: 1210 4302
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2066 4123
Size: 107 122 71 631 605 337 57 2057
Cuts at: 4123 136
Size: 1525
Fragments arranged by size:
2057 1525 631 605 337 122 107 71
57
BglII A'GATC_T
Cuts at: 5507 5507
Size: 5512
BlpI GC'TnA_GC
Cuts at: 2929 2929
Size: 5512
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2163 2291 3573 4729 1790
Size: 261 112 128 1282 1156 2573
Fragments arranged by size:
2573 1282 1156 261 128 112
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5512
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2422 3243 4201
Size: 416 53 83 399 24 1005 821 958
Cuts at: 4201 442
Size: 1753
Fragments arranged by size:
1753 1005 958 821 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4172 1239
Size: 2933 2579
Bpu10I CC'TnA_GC
Cuts at: 2655 2655
Size: 5512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3637 4502 4800 3637
Size: 865 298 4349
Fragments arranged by size:
4349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4175 882
Size: 617 2676 2219
Fragments arranged by size:
2676 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3303 493
Size: 711 2099 2702
Fragments arranged by size:
2702 2099 711
BsaBI GATnn'nnATC
Cuts at: 5506 5506
Size: 5512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2715 2733 3319 3701
Size: 53 83 186 936 1182 18 586 382
Cuts at: 3701 4336 275
Size: 635 1451
Fragments arranged by size:
1451 1182 936 635 586 382 186 83
53 18
BsaWI w'CCGG_w
Cuts at: 2553 3945 4343 4617 4764 5175 2553
Size: 1392 398 274 147 411 2890
Fragments arranged by size:
2890 1392 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2045 2075 2090 2120 1178
Size: 30 165 30 642 30 15 30 4570
Fragments arranged by size:
4570 642 165 30 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 1186
Size: 922 4590
BseYI C'CCAG_C
Cuts at: 2029 2044 4715 5211 2029
Size: 15 2671 496 2330
Fragments arranged by size:
2671 2330 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2727 1163
Size: 573 991 3948
Fragments arranged by size:
3948 991 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2962 3723 4751 1540
Size: 275 186 961 761 1028 2301
Fragments arranged by size:
2301 1028 961 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3573 3658 4729 3573
Size: 85 1071 4356
Fragments arranged by size:
4356 1071 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
BsmBI CGTCTCn'nnnn_
Cuts at: 2357 2357
Size: 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2163 2283 2291 2582 2831 3573
Size: 399 261 112 120 8 291 249 742
Cuts at: 3573 3658 4729 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 291 261 249 120
112 85 8
BspEI T'CCGG_A
Cuts at: 5175 5175
Size: 5512
BspHI T'CATG_A
Cuts at: 2454 3401 5131 2454
Size: 947 1730 2835
Fragments arranged by size:
2835 1730 947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2132 2463 2867 844
Size: 1288 331 404 3489
Fragments arranged by size:
3489 1288 404 331
BsrBI CCG'CTC
Cuts at: 1394 1823 3399 1394
Size: 429 1576 3507
Fragments arranged by size:
3507 1576 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4007 4181 65
Size: 1183 2759 174 1396
Fragments arranged by size:
2759 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2072 2499 2553 2735 4156 4343 1700
Size: 110 262 427 54 182 1421 187 2869
Fragments arranged by size:
2869 1421 427 262 187 182 110 54
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5512
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5512
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5512
BstBI TT'CG_AA
Cuts at: 4316 4316
Size: 5512
BstEII G'GTnAC_C
Cuts at: 1184 2671 1184
Size: 1487 4025
BstYI r'GATC_y
Cuts at: 1972 2842 3209 3594 3611 5063 5149 5161 5507
Size: 870 367 385 17 1452 86 12 346
Cuts at: 5507 1972
Size: 1977
Fragments arranged by size:
1977 1452 870 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2053 2283 2308 513
Size: 552 939 49 230 25 3717
Fragments arranged by size:
3717 939 552 230 49 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4773
Fragments arranged by size:
4773 683 56
BtsI GCAGTG_nn'
Cuts at: 3028 3822 3842 3028
Size: 794 20 4698
Fragments arranged by size:
4698 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2816 2816
Size: 5512
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4519 809
Size: 507 3203 1802
Fragments arranged by size:
3203 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2209 2292 2367
Size: 54 1425 50 275 18 379 83 75
Cuts at: 2367 2545 2622 2959 3848 5477 8
Size: 178 77 337 889 1629 43
Fragments arranged by size:
1629 1425 889 379 337 275 178 83
77 75 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2959 1537
Size: 275 1147 4090
Fragments arranged by size:
4090 1147 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2747 2812 3442 1353
Size: 1394 65 630 3423
Fragments arranged by size:
3423 1394 630 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2783 4099 4473 4619 387
Size: 1064 138 1194 1316 374 146 1280
Fragments arranged by size:
1316 1280 1194 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2210 2771 3575 4172 4959 5227
Size: 393 560 18 561 804 597 787 268
Cuts at: 5227 1239
Size: 1524
Fragments arranged by size:
1524 804 787 597 561 560 393 268
18
EcoO109I rG'GnC_Cy
Cuts at: 2449 2449
Size: 5512
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FspI TGC'GCA
Cuts at: 2612 4018 2612
Size: 1406 4106
HaeII r_GCGC'y
Cuts at: 1678 2145 2701 4659 1678
Size: 467 556 1958 2531
Fragments arranged by size:
2531 1958 556 467
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2723 2755 4153
Size: 32 135 32 518 32 601 32 1398
Cuts at: 4153 4185 4227 4259 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1398 601 518 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2856 3102 669
Size: 988 281 918 246 3079
Fragments arranged by size:
3079 988 918 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5512
HpaI GTT'AAC
Cuts at: 3102 3102
Size: 5512
KpnI G_GTAC'C
Cuts at: 2837 2837
Size: 5512
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 5512
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2607 4626 4810 1305
Size: 1302 2019 184 2007
Fragments arranged by size:
2019 2007 1302 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3690
Fragments arranged by size:
3690 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2772 3470 3829 3988 518
Size: 648 1606 698 359 159 2042
Fragments arranged by size:
2042 1606 698 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2166 2760 2928 3605 4753 4998 1606
Size: 560 594 168 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 594 560 245 168
NaeI GCC'GGC
Cuts at: 2074 2737 2074
Size: 663 4849
NcoI C'CATG_G
Cuts at: 513 1065 2004 2283 513
Size: 552 939 279 3742
Fragments arranged by size:
3742 939 552 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5512
NgoMIV G'CCGG_C
Cuts at: 2072 2735 2072
Size: 663 4849
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2959 2959
Size: 5512
NspI r_CATG'y
Cuts at: 1132 1229 2870 4415 1132
Size: 97 1641 1545 2229
Fragments arranged by size:
2229 1641 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5512
PciI A'CATG_T
Cuts at: 4411 4411
Size: 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5512
PfoI T'CCnGG_A
Cuts at: 2718 2718
Size: 5512
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2459 2491 3581 3613 5094 5126 2459
Size: 32 1090 32 1481 32 2845
Fragments arranged by size:
2845 1481 1090 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5512
PsiI TTA'TAA
Cuts at: 3082 3082
Size: 5512
PstI C_TGCA'G
Cuts at: 830 2864 830
Size: 2034 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SapI GCTCTTCn'nnn_
Cuts at: 2747 2747
Size: 5512
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 2258 3760 5472 2258
Size: 1502 1712 2298
Fragments arranged by size:
2298 1712 1502
SfcI C'TryA_G
Cuts at: 826 1045 2860 3995 4676 4867 826
Size: 219 1815 1135 681 191 1471
Fragments arranged by size:
1815 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2839 1872
Size: 967 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3616 4517 4779 820
Size: 197 926 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5512
SphI G_CATG'C
Cuts at: 1132 2870 1132
Size: 1738 3774
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5512
StyI C'CwwG_G
Cuts at: 513 1065 2004 2101 2283 2574 2951 513
Size: 552 939 97 182 291 377 3074
Fragments arranged by size:
3074 939 552 377 291 182 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2066 3539 3556 3709 3894 4339 325
Size: 1255 486 1473 17 153 185 445 1498
Fragments arranged by size:
1498 1473 1255 486 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2256 3758 5470
Size: 274 80 33 51 1443 279 1502 1712
Cuts at: 5470 96
Size: 138
Fragments arranged by size:
1712 1502 1443 279 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2045 2077 2231 2263 5472 5504 2045
Size: 32 154 32 3209 32 2053
Fragments arranged by size:
3209 2053 154 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5512
XbaI T'CTAG_A
Cuts at: 2848 2848
Size: 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2837 1870
Size: 967 4545
XmnI GAAnn'nnTTC
Cuts at: 3641 3641
Size: 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2716 3320 276
Size: 53 83 186 936 1182 604 2468
Fragments arranged by size:
2468 1182 936 604 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI