Restriction map for
pFN21AB8264
(Circular) (Six-base) MAPSORT of: pFN21AB8264.seq Check: 4099 from: 1 to: 5335
pFN21AB8264 5335 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2560 2560
Size: 5335
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3145 278
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2361 2678 1937
Size: 424 317 4594
Fragments arranged by size:
4594 424 317
Acc65I G'GTAC_C
Cuts at: 2656 2656
Size: 5335
AclI AA'CG_TT
Cuts at: 1563 3462 3835 1563
Size: 1899 373 3063
Fragments arranged by size:
3063 1899 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3398 4782 5050 1632
Size: 1766 1384 268 1917
Fragments arranged by size:
1917 1766 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5138
AflIII A'CryG_T
Cuts at: 1203 4234 5211 1203
Size: 3031 977 1327
Fragments arranged by size:
3031 1327 977
AgeI A'CCGG_T
Cuts at: 4166 4166
Size: 5335
AhdI GACnn_n'nnGTC
Cuts at: 4064 4064
Size: 5335
AleI CACnn'nnGTG
Cuts at: 1166 2073 1166
Size: 907 4428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5335
AlwNI CAG_nnn'CTG
Cuts at: 2113 2356 2395 2407 3078 4650 2113
Size: 243 39 12 671 1572 2798
Fragments arranged by size:
2798 1572 671 243 39 12
ApaLI G'TGCA_C
Cuts at: 3392 4548 3392
Size: 1156 4179
ApoI r'AATT_y
Cuts at: 1493 2644 2870 3022 5131 1493
Size: 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3889 160
Size: 872 2857 1606
Fragments arranged by size:
2857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
AvaI C'yCGr_G
Cuts at: 1870 1943 2660 1870
Size: 73 717 4545
Fragments arranged by size:
4545 717 73
BamHI G'GATC_C
Cuts at: 2520 2665 3032 2520
Size: 145 367 4823
Fragments arranged by size:
4823 367 145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2656 4111 618
Size: 325 217 627 869 1455 1842
Fragments arranged by size:
1842 1455 869 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2244 2311 2654 1391
Size: 853 67 343 4072
Fragments arranged by size:
4072 853 343 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3526 3560 1903
Size: 34 1589 34 3678
Fragments arranged by size:
3678 1589 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3227 4437 3227
Size: 1210 4125
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3946 136
Size: 107 122 71 631 605 2274 1525
Fragments arranged by size:
2274 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5330 5330
Size: 5335
BlpI GC'TnA_GC
Cuts at: 2752 2752
Size: 5335
Bme1580I G_kGCm'C
Cuts at: 1790 3396 4552 1790
Size: 1606 1156 2573
Fragments arranged by size:
2573 1606 1156
BmgBI CAC'GTC
Cuts at: 1318 2449 1318
Size: 1131 4204
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2586 3066 4024
Size: 416 53 83 399 24 1169 480 958
Cuts at: 4024 442
Size: 1753
Fragments arranged by size:
1753 1169 958 480 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2405 3995 1239
Size: 896 270 1590 2579
Fragments arranged by size:
2579 1590 896 270
Bpu10I CC'TnA_GC
Cuts at: 2442 2442
Size: 5335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3460 4325 4623 3460
Size: 865 298 4172
Fragments arranged by size:
4172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3998 882
Size: 617 2499 2219
Fragments arranged by size:
2499 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3126 493
Size: 711 1922 2702
Fragments arranged by size:
2702 1922 711
BsaBI GATnn'nnATC
Cuts at: 5329 5329
Size: 5335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3142 3524 4159 275
Size: 53 83 186 936 1609 382 635 1451
Fragments arranged by size:
1609 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3768 4166 4440 4587 4998 3768
Size: 398 274 147 411 4105
Fragments arranged by size:
4105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 1178
Size: 30 165 30 606 30 4474
Fragments arranged by size:
4474 606 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2033 2132 2252 2306 2480 1186
Size: 844 3 99 120 54 174 4041
Fragments arranged by size:
4041 844 174 120 99 54 3
BseYI C'CCAG_C
Cuts at: 4538 5034 4538
Size: 496 4839
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 2651 1163
Size: 573 606 309 3847
Fragments arranged by size:
3847 606 573 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2074 2162 2785 3546 4574 1540
Size: 275 186 73 88 623 761 1028 2301
Fragments arranged by size:
2301 1028 761 623 275 186 88 73
BsiHKAI G_wGCw'C
Cuts at: 2430 2448 3396 3481 4552 2430
Size: 18 948 85 1071 3213
Fragments arranged by size:
3213 1071 948 85 18
BsmI GAATG_Cn'
Cuts at: 2846 2939 2846
Size: 93 5242
BsmBI CGTCTCn'nnnn_
Cuts at: 2015 2591 2015
Size: 576 4759
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2311 2430 2448 2654 3396 3481
Size: 399 454 67 119 18 206 742 85
Cuts at: 3481 4552 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 206 119 85
67 18
BspEI T'CCGG_A
Cuts at: 4998 4998
Size: 5335
BspHI T'CATG_A
Cuts at: 2454 3224 4954 2454
Size: 770 1730 2835
Fragments arranged by size:
2835 1730 770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2560 2598 2623 2690 844
Size: 1716 38 25 67 3489
Fragments arranged by size:
3489 1716 67 38 25
BsrBI CCG'CTC
Cuts at: 1394 1823 3222 1394
Size: 429 1399 3507
Fragments arranged by size:
3507 1399 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3830 4004 65
Size: 1183 2582 174 1396
Fragments arranged by size:
2582 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2028 2622 3979 4166 1700
Size: 110 218 594 1357 187 2869
Fragments arranged by size:
2869 1357 594 218 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5335
BssHII G'CGCG_C
Cuts at: 1925 2543 1925
Size: 618 4717
BssSI C'ACGA_G
Cuts at: 2429 3395 4407 2429
Size: 966 1012 3357
Fragments arranged by size:
3357 1012 966
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2395 2407 794
Size: 1601 12 3722
Fragments arranged by size:
3722 1601 12
BstBI TT'CG_AA
Cuts at: 4139 4139
Size: 5335
BstEII G'GTnAC_C
Cuts at: 1184 2086 1184
Size: 902 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 2075 2075
Size: 5335
BstYI r'GATC_y
Cuts at: 1972 2520 2665 3032 3417 3434 4886 4972 4984
Size: 548 145 367 385 17 1452 86 12
Cuts at: 4984 5330 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 548 385 367 346 145 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2074 2162 513
Size: 552 939 70 88 3686
Fragments arranged by size:
3686 939 552 88 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4596
Fragments arranged by size:
4596 683 56
BtsI GCAGTG_nn'
Cuts at: 2851 3645 3665 2851
Size: 794 20 4521
Fragments arranged by size:
4521 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5300
DraI TTT'AAA
Cuts at: 2639 2639
Size: 5335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4342 809
Size: 507 3026 1802
Fragments arranged by size:
3026 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2159 2782 3671
Size: 54 1425 50 275 18 329 623 889
Cuts at: 3671 5300 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 623 329 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2159 2782 1537
Size: 275 347 623 4090
Fragments arranged by size:
4090 623 347 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3265 1353
Size: 1912 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2332 3922 4296 4442 387
Size: 1064 138 743 1590 374 146 1280
Fragments arranged by size:
1590 1280 1064 743 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2405 3398 3995 4782 5050 1239
Size: 393 503 270 993 597 787 268 1524
Fragments arranged by size:
1524 993 787 597 503 393 270 268
EcoO109I rG'GnC_Cy
Cuts at: 2533 2533
Size: 5335
EcoRI G'AATT_C
Cuts at: 2644 3022 2644
Size: 378 4957
FspI TGC'GCA
Cuts at: 3841 3841
Size: 5335
HaeII r_GCGC'y
Cuts at: 1678 4482 1678
Size: 2804 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2481 2513 3976 4008 4050
Size: 32 135 32 909 32 1463 32 42
Cuts at: 4050 4082 1373
Size: 32 2626
Fragments arranged by size:
2626 1463 909 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 2679 2925 669
Size: 988 281 424 317 246 3079
Fragments arranged by size:
3079 988 424 317 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5335
HpaI GTT'AAC
Cuts at: 2925 2925
Size: 5335
KpnI G_GTAC'C
Cuts at: 2660 2660
Size: 5335
MfeI C'AATT_G
Cuts at: 2934 2934
Size: 5335
MluI A'CGCG_T
Cuts at: 5211 5211
Size: 5335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4449 4633 1305
Size: 3144 184 2007
Fragments arranged by size:
3144 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3513
Fragments arranged by size:
3513 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2073 2199 2267 2318 3293 3652 3811
Size: 648 907 126 68 51 975 359 159
Cuts at: 3811 518
Size: 2042
Fragments arranged by size:
2042 975 907 648 359 159 126 68
51
MspA1I CmG'CkG
Cuts at: 1606 2062 2164 2512 2751 3428 4576 4821 1606
Size: 456 102 348 239 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 456 348 245 239 102
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3844
Fragments arranged by size:
3844 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5335
NotI GC'GGCC_GC
Cuts at: 2159 2782 2159
Size: 623 4712
NspI r_CATG'y
Cuts at: 1132 1229 2287 2693 4238 1132
Size: 97 1058 406 1545 2229
Fragments arranged by size:
2229 1545 1058 406 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5335
PciI A'CATG_T
Cuts at: 4234 4234
Size: 5335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2113 1108
Size: 1005 4330
PfoI T'CCnGG_A
Cuts at: 2382 2382
Size: 5335
PmeI GTTT'AAAC
Cuts at: 2639 2639
Size: 5335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 4917 4949 3404
Size: 32 1481 32 3790
Fragments arranged by size:
3790 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2533 2533
Size: 5335
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5335
PsiI TTA'TAA
Cuts at: 2905 2905
Size: 5335
PstI C_TGCA'G
Cuts at: 830 2400 2687 830
Size: 1570 287 3478
Fragments arranged by size:
3478 1570 287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5335
PvuII CAG'CTG
Cuts at: 2062 2512 2062
Size: 450 4885
SacII CC_GC'GG
Cuts at: 2165 2165
Size: 5335
SalI G'TCGA_C
Cuts at: 1936 2360 2677 1936
Size: 424 317 4594
Fragments arranged by size:
4594 424 317
SanDI GG'GwC_CC
Cuts at: 2533 2533
Size: 5335
SbfI CC_TGCA'GG
Cuts at: 2687 2687
Size: 5335
ScaI AGT'ACT
Cuts at: 3583 5295 3583
Size: 1712 3623
SfcI C'TryA_G
Cuts at: 826 1045 2396 2683 3818 4499 4690 826
Size: 219 1351 287 1135 681 191 1471
Fragments arranged by size:
1471 1351 1135 681 287 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5335
SmaI CCC'GGG
Cuts at: 1872 2662 1872
Size: 790 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3439 4340 4602 820
Size: 197 926 1496 901 262 1553
Fragments arranged by size:
1553 1496 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3126 493
Size: 2633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5335
SphI G_CATG'C
Cuts at: 1132 2287 2693 1132
Size: 1155 406 3774
Fragments arranged by size:
3774 1155 406
SspI AAT'ATT
Cuts at: 5 52 3259 5
Size: 47 3207 2081
Fragments arranged by size:
3207 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5335
StyI C'CwwG_G
Cuts at: 513 1065 2004 2244 2460 2774 513
Size: 552 939 240 216 314 3074
Fragments arranged by size:
3074 939 552 314 240 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3362 3379 3532 3717 4162 325
Size: 1255 1782 17 153 185 445 1498
Fragments arranged by size:
1782 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 2376 3581
Size: 274 80 33 51 1443 294 105 1205
Cuts at: 3581 5293 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1205 294 274 138 105 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2548 2580 5295 5327 2548
Size: 32 2715 32 2556
Fragments arranged by size:
2715 2556 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5335
XbaI T'CTAG_A
Cuts at: 2671 2671
Size: 5335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2353 2380 2353
Size: 27 5308
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5335
XmaI C'CCGG_G
Cuts at: 1870 2660 1870
Size: 790 4545
XmnI GAAnn'nnTTC
Cuts at: 3464 3464
Size: 5335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3143 276
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SapI SexAI SfiI SfoI SrfI SwaI