Restriction map for
pFN21AB8260
(Circular) (Six-base) MAPSORT of: pFN21AB8260.seq Check: 7070 from: 1 to: 5302
pFN21AB8260 5302 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3112 278
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2645 1937
Size: 708 4594
Acc65I G'GTAC_C
Cuts at: 2623 2623
Size: 5302
AclI AA'CG_TT
Cuts at: 1563 3429 3802 1563
Size: 1866 373 3063
Fragments arranged by size:
3063 1866 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 2397 3365 4749 5017 1632
Size: 641 124 968 1384 268 1917
Fragments arranged by size:
1917 1384 968 641 268 124
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5105
AflIII A'CryG_T
Cuts at: 1203 4201 5178 1203
Size: 2998 977 1327
Fragments arranged by size:
2998 1327 977
AgeI A'CCGG_T
Cuts at: 4133 4133
Size: 5302
AhdI GACnn_n'nnGTC
Cuts at: 4031 4031
Size: 5302
AleI CACnn'nnGTG
Cuts at: 1166 2471 1166
Size: 1305 3997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2489 2540 1184
Size: 1305 51 3946
Fragments arranged by size:
3946 1305 51
AlwNI CAG_nnn'CTG
Cuts at: 2586 3045 4617 2586
Size: 459 1572 3271
Fragments arranged by size:
3271 1572 459
ApaI G_GGCC'C
Cuts at: 2075 2075
Size: 5302
ApaLI G'TGCA_C
Cuts at: 2297 3359 4515 2297
Size: 1062 1156 3084
Fragments arranged by size:
3084 1156 1062
ApoI r'AATT_y
Cuts at: 1493 2611 2837 2989 5098 1493
Size: 1118 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3856 160
Size: 872 2824 1606
Fragments arranged by size:
2824 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
AvaI C'yCGr_G
Cuts at: 1870 1943 2627 1870
Size: 73 684 4545
Fragments arranged by size:
4545 684 73
BamHI G'GATC_C
Cuts at: 2153 2632 2999 2153
Size: 479 367 4456
Fragments arranged by size:
4456 479 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 2365 2465 2508 2552
Size: 325 217 627 539 39 100 43 44
Cuts at: 2552 2623 4078 618
Size: 71 1455 1842
Fragments arranged by size:
1842 1455 627 539 325 217 100 71
44 43 39
BanII G_rGCy'C
Cuts at: 1391 2075 2621 1391
Size: 684 546 4072
Fragments arranged by size:
4072 684 546
BbeI G_GCGC'C
Cuts at: 2556 2556
Size: 5302
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4331
BbvCI CC'TCA_GC
Cuts at: 2357 2357
Size: 5302
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3493 3527 1903
Size: 34 1556 34 3678
Fragments arranged by size:
3678 1556 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2132 3194 4404 2132
Size: 1062 1210 3030
Fragments arranged by size:
3030 1210 1062
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 3913 136
Size: 107 122 71 631 605 365 1876 1525
Fragments arranged by size:
1876 1525 631 605 365 122 107 71
BglII A'GATC_T
Cuts at: 5297 5297
Size: 5302
BlpI GC'TnA_GC
Cuts at: 2306 2719 2306
Size: 413 4889
Bme1580I G_kGCm'C
Cuts at: 1790 2075 2301 2370 3363 4519 1790
Size: 285 226 69 993 1156 2573
Fragments arranged by size:
2573 1156 993 285 226 69
BmgBI CAC'GTC
Cuts at: 1318 2167 1318
Size: 849 4453
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2288 3033 3991
Size: 416 53 83 399 24 871 745 958
Cuts at: 3991 442
Size: 1753
Fragments arranged by size:
1753 958 871 745 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2447 1056
Size: 1391 3911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3962 1239
Size: 2723 2579
Bpu10I CC'TnA_GC
Cuts at: 2357 2357
Size: 5302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 3427 4292 4590 2333
Size: 1094 865 298 3045
Fragments arranged by size:
3045 1094 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3965 882
Size: 617 2466 2219
Fragments arranged by size:
2466 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3093 493
Size: 711 1889 2702
Fragments arranged by size:
2702 1889 711
BsaBI GATnn'nnATC
Cuts at: 5296 5296
Size: 5302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2421 2553 3109 3491
Size: 53 83 186 936 888 132 556 382
Cuts at: 3491 4126 275
Size: 635 1451
Fragments arranged by size:
1451 936 888 635 556 382 186 132
83 53
BsaWI w'CCGG_w
Cuts at: 2065 2115 3735 4133 4407 4554 4965 2065
Size: 50 1620 398 274 147 411 2402
Fragments arranged by size:
2402 1620 411 398 274 147 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5077
Fragments arranged by size:
5077 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2345 2447 1186
Size: 1159 102 4041
Fragments arranged by size:
4041 1159 102
BseYI C'CCAG_C
Cuts at: 2336 4505 5001 2336
Size: 2169 496 2637
Fragments arranged by size:
2637 2169 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2246 2327 2507 1163
Size: 573 510 81 180 3958
Fragments arranged by size:
3958 573 510 180 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2752 3513 4541 1540
Size: 275 186 751 761 1028 2301
Fragments arranged by size:
2301 1028 761 751 275 186
BsiHKAI G_wGCw'C
Cuts at: 2301 2580 3363 3448 4519 2301
Size: 279 783 85 1071 3084
Fragments arranged by size:
3084 1071 783 279 85
BsmI GAATG_Cn'
Cuts at: 2813 2906 2813
Size: 93 5209
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2301 2370 2580 2621 3363 3448
Size: 399 285 226 69 210 41 742 85
Cuts at: 3448 4519 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 285 226 210 85
69 41
BspEI T'CCGG_A
Cuts at: 2115 4965 2115
Size: 2850 2452
BspHI T'CATG_A
Cuts at: 3191 4921 3191
Size: 1730 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2657 844
Size: 1813 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3189 1394
Size: 429 1366 3507
Fragments arranged by size:
3507 1366 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2088 3797 3971 65
Size: 1183 840 1709 174 1396
Fragments arranged by size:
1709 1396 1183 840 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2505 3946 4133 1700
Size: 110 695 1441 187 2869
Fragments arranged by size:
2869 1441 695 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5302
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5302
BssSI C'ACGA_G
Cuts at: 3362 4374 3362
Size: 1012 4290
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5302
BstBI TT'CG_AA
Cuts at: 4106 4106
Size: 5302
BstEII G'GTnAC_C
Cuts at: 1184 2206 1184
Size: 1022 4280
BstXI CCAn_nnnn'nTGG
Cuts at: 2530 2530
Size: 5302
BstYI r'GATC_y
Cuts at: 1972 2153 2256 2632 2999 3384 3401 4853 4939
Size: 181 103 376 367 385 17 1452 86
Cuts at: 4939 4951 5297 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 376 367 346 181 103
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2512 513
Size: 552 939 508 3303
Fragments arranged by size:
3303 939 552 508
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4563
Fragments arranged by size:
4563 683 56
BtsI GCAGTG_nn'
Cuts at: 2818 3612 3632 2818
Size: 794 20 4488
Fragments arranged by size:
4488 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5267
DraI TTT'AAA
Cuts at: 2606 2606
Size: 5302
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2198 4309 809
Size: 507 882 2111 1802
Fragments arranged by size:
2111 1802 882 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2749 3638 5267
Size: 54 1425 50 275 18 919 889 1629
Cuts at: 5267 8
Size: 43
Fragments arranged by size:
1629 1425 919 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2749 1537
Size: 275 937 4090
Fragments arranged by size:
4090 937 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 3232 1353
Size: 714 1165 3423
Fragments arranged by size:
3423 1165 714
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3889 4263 4409 387
Size: 1064 138 2300 374 146 1280
Fragments arranged by size:
2300 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2397 3365 3962 4749 5017 1239
Size: 393 641 124 968 597 787 268 1524
Fragments arranged by size:
1524 968 787 641 597 393 268 124
EcoNI CCTnn'n_nnAGG
Cuts at: 2429 2429
Size: 5302
EcoO109I rG'GnC_Cy
Cuts at: 2072 2072
Size: 5302
EcoRI G'AATT_C
Cuts at: 2611 2989 2611
Size: 378 4924
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 5302
FspI TGC'GCA
Cuts at: 3808 3808
Size: 5302
HaeII r_GCGC'y
Cuts at: 1678 2556 4449 1678
Size: 878 1893 2531
Fragments arranged by size:
2531 1893 878
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3943 3975 4017 4049 1373
Size: 32 135 32 2371 32 42 32 2626
Fragments arranged by size:
2626 2371 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2646 2892 669
Size: 988 281 708 246 3079
Fragments arranged by size:
3079 988 708 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5302
HpaI GTT'AAC
Cuts at: 2892 2892
Size: 5302
KasI G'GCGC_C
Cuts at: 2552 2552
Size: 5302
KpnI G_GTAC'C
Cuts at: 2627 2627
Size: 5302
MfeI C'AATT_G
Cuts at: 2901 2901
Size: 5302
MluI A'CGCG_T
Cuts at: 5178 5178
Size: 5302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4416 4600 1305
Size: 3111 184 2007
Fragments arranged by size:
3111 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3480
Fragments arranged by size:
3480 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 2268 2471 2522 3260 3619 3778
Size: 648 1009 93 203 51 738 359 159
Cuts at: 3778 518
Size: 2042
Fragments arranged by size:
2042 1009 738 648 359 203 159 93
51
MspA1I CmG'CkG
Cuts at: 1606 2718 3395 4543 4788 1606
Size: 1112 677 1148 245 2120
Fragments arranged by size:
2120 1148 1112 677 245
NarI GG'CG_CC
Cuts at: 2553 2553
Size: 5302
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3811
Fragments arranged by size:
3811 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5302
NheI G'CTAG_C
Cuts at: 1052 2443 1052
Size: 1391 3911
NotI GC'GGCC_GC
Cuts at: 2749 2749
Size: 5302
NspI r_CATG'y
Cuts at: 1132 1229 2660 4205 1132
Size: 97 1431 1545 2229
Fragments arranged by size:
2229 1545 1431 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5302
PciI A'CATG_T
Cuts at: 4201 4201
Size: 5302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5302
PmeI GTTT'AAAC
Cuts at: 2606 2606
Size: 5302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 4884 4916 3371
Size: 32 1481 32 3757
Fragments arranged by size:
3757 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5302
PsiI TTA'TAA
Cuts at: 2872 2872
Size: 5302
PspOMI G'GGCC_C
Cuts at: 2071 2071
Size: 5302
PstI C_TGCA'G
Cuts at: 830 2014 2098 2654 830
Size: 1184 84 556 3478
Fragments arranged by size:
3478 1184 556 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5302
SalI G'TCGA_C
Cuts at: 1936 2644 1936
Size: 708 4594
SapI GCTCTTCn'nnn_
Cuts at: 2067 2067
Size: 5302
SbfI CC_TGCA'GG
Cuts at: 2654 2654
Size: 5302
ScaI AGT'ACT
Cuts at: 2402 3550 5262 2402
Size: 1148 1712 2442
Fragments arranged by size:
2442 1712 1148
SfcI C'TryA_G
Cuts at: 826 1045 2010 2094 2650 3785 4466 4657 826
Size: 219 965 84 556 1135 681 191 1471
Fragments arranged by size:
1471 1135 965 681 556 219 191 84
SfoI GGC'GCC
Cuts at: 2554 2554
Size: 5302
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5302
SmaI CCC'GGG
Cuts at: 1872 2629 1872
Size: 757 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 3406 4307 4569 820
Size: 197 926 405 1058 901 262 1553
Fragments arranged by size:
1553 1058 926 901 405 262 197
SnaBI TAC'GTA
Cuts at: 493 3093 493
Size: 2600 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5302
SphI G_CATG'C
Cuts at: 1132 2660 1132
Size: 1528 3774
SspI AAT'ATT
Cuts at: 5 52 3226 5
Size: 47 3174 2081
Fragments arranged by size:
3174 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5302
StyI C'CwwG_G
Cuts at: 513 1065 2004 2741 513
Size: 552 939 737 3074
Fragments arranged by size:
3074 939 737 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2225 3329 3346 3499 3684 4129 325
Size: 1255 645 1104 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1104 645 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 3548 5260
Size: 274 80 33 51 1443 423 1148 1712
Cuts at: 5260 96
Size: 138
Fragments arranged by size:
1712 1443 1148 423 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5262 5294 5262
Size: 32 5270
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5302
XbaI T'CTAG_A
Cuts at: 2638 2638
Size: 5302
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5302
XmaI C'CCGG_G
Cuts at: 1870 2627 1870
Size: 757 4545
XmnI GAAnn'nnTTC
Cuts at: 3431 3431
Size: 5302
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3110 276
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI XcmI