Restriction map for
pFN21AB8247
(Circular) (Six-base) MAPSORT of: pFN21AB8247.seq Check: 7699 from: 1 to: 5476
pFN21AB8247 5476 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3286 278
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2077 2397 2819 1937
Size: 140 320 422 4594
Fragments arranged by size:
4594 422 320 140
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 3603 3976 1563
Size: 2040 373 3063
Fragments arranged by size:
3063 2040 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3539 4923 5191 1632
Size: 1907 1384 268 1917
Fragments arranged by size:
1917 1907 1384 268
AfeI AGC'GCT
Cuts at: 2222 2222
Size: 5476
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 4375 5352 1203
Size: 3172 977 1327
Fragments arranged by size:
3172 1327 977
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 4205 4205
Size: 5476
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5476
AlwNI CAG_nnn'CTG
Cuts at: 3219 4791 3219
Size: 1572 3904
ApaI G_GGCC'C
Cuts at: 2182 2660 2182
Size: 478 4998
ApaLI G'TGCA_C
Cuts at: 3533 4689 3533
Size: 1156 4320
ApoI r'AATT_y
Cuts at: 1493 2785 3011 3163 5272 1493
Size: 1292 226 152 2109 1697
Fragments arranged by size:
2109 1697 1292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2273 2801 1870
Size: 73 330 528 4545
Fragments arranged by size:
4545 528 330 73
BamHI G'GATC_C
Cuts at: 2592 2806 3173 2592
Size: 214 367 4895
Fragments arranged by size:
4895 367 214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2797 4252 618
Size: 325 217 627 1010 1455 1842
Fragments arranged by size:
1842 1455 1010 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2103 2182 2660 2795 1391
Size: 712 79 478 135 4072
Fragments arranged by size:
4072 712 478 135 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2709 928
Size: 971 810 3695
Fragments arranged by size:
3695 971 810
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3667 3701 1903
Size: 34 1730 34 3678
Fragments arranged by size:
3678 1730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4087 136
Size: 107 122 71 631 605 2415 1525
Fragments arranged by size:
2415 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2893 2893
Size: 5476
Bme1580I G_kGCm'C
Cuts at: 1790 2182 2660 3537 4693 1790
Size: 392 478 877 1156 2573
Fragments arranged by size:
2573 1156 877 478 392
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2405 3207 4165
Size: 416 53 83 399 24 988 802 958
Cuts at: 4165 442
Size: 1753
Fragments arranged by size:
1753 988 958 802 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2189 2441 2783 4136 1239
Size: 878 72 252 342 1353 2579
Fragments arranged by size:
2579 1353 878 342 252 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2003 2066 3601 4466 4764 2003
Size: 63 1535 865 298 2715
Fragments arranged by size:
2715 1535 865 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4139 882
Size: 617 2640 2219
Fragments arranged by size:
2640 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3267 493
Size: 711 2063 2702
Fragments arranged by size:
2702 2063 711
BsaBI GATnn'nnATC
Cuts at: 5470 5470
Size: 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3283 3665 4300 275
Size: 53 83 186 936 1750 382 635 1451
Fragments arranged by size:
1750 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3909 4307 4581 4728 5139 3909
Size: 398 274 147 411 4246
Fragments arranged by size:
4246 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2195 2225 1178
Size: 30 165 30 792 30 4429
Fragments arranged by size:
4429 792 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 1186
Size: 1117 4359
BseYI C'CCAG_C
Cuts at: 2071 2232 2540 4679 5175 2071
Size: 161 308 2139 496 2372
Fragments arranged by size:
2372 2139 496 308 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4903
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2926 3687 4715 1540
Size: 275 186 925 761 1028 2301
Fragments arranged by size:
2301 1028 925 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2103 3537 3622 4693 2103
Size: 1434 85 1071 2886
Fragments arranged by size:
2886 1434 1071 85
BsmI GAATG_Cn'
Cuts at: 2987 3080 2987
Size: 93 5383
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2182 2660 2795 3537 3622 4693
Size: 399 313 79 478 135 742 85 1071
Cuts at: 4693 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 478 399 313 135 85
79
BspEI T'CCGG_A
Cuts at: 5139 5139
Size: 5476
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2831 844
Size: 1987 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2365 3363 1394
Size: 429 542 998 3507
Fragments arranged by size:
3507 998 542 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3971 4145 65
Size: 1183 2723 174 1396
Fragments arranged by size:
2723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4120 4307 1700
Size: 110 2310 187 2869
Fragments arranged by size:
2869 2310 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5476
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5476
BssSI C'ACGA_G
Cuts at: 3536 4548 3536
Size: 1012 4464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5476
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5476
BstXI CCAn_nnnn'nTGG
Cuts at: 2728 2728
Size: 5476
BstYI r'GATC_y
Cuts at: 1972 2592 2806 3173 3558 3575 5027 5113 5125
Size: 620 214 367 385 17 1452 86 12
Cuts at: 5125 5471 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 620 385 367 346 214 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4924
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4737
Fragments arranged by size:
4737 683 56
BtsI GCAGTG_nn'
Cuts at: 2992 3786 3806 2992
Size: 794 20 4662
Fragments arranged by size:
4662 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2780 2780
Size: 5476
DraIII CAC_nnn'GTG
Cuts at: 2413 2413
Size: 5476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4483 809
Size: 507 3167 1802
Fragments arranged by size:
3167 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2463 2730 2923
Size: 54 1425 50 275 18 633 267 193
Cuts at: 2923 3812 5441 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 633 275 267 193 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2923 1537
Size: 275 1111 4090
Fragments arranged by size:
4090 1111 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2061 2321 2603 3406 1353
Size: 708 260 282 803 3423
Fragments arranged by size:
3423 803 708 282 260
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4063 4437 4583 387
Size: 1064 138 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2101 2101
Size: 5476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2189 2441 2783 3539 4136 4923
Size: 393 485 72 252 342 756 597 787
Cuts at: 4923 5191 1239
Size: 268 1524
Fragments arranged by size:
1524 787 756 597 485 393 342 268
252 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2198 2435 2465 2198
Size: 237 30 5209
Fragments arranged by size:
5209 237 30
EcoO109I rG'GnC_Cy
Cuts at: 2178 2650 2657 2178
Size: 472 7 4997
Fragments arranged by size:
4997 472 7
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 2224 4623 1678
Size: 546 2399 2531
Fragments arranged by size:
2531 2399 546
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2634 2666 4117
Size: 32 135 32 623 32 407 32 1451
Cuts at: 4117 4149 4191 4223 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1451 623 407 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2078 2820 3066 669
Size: 988 281 140 742 246 3079
Fragments arranged by size:
3079 988 742 281 246 140
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2340 4590 4774 1305
Size: 1035 2250 184 2007
Fragments arranged by size:
2250 2007 1035 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2465 2732 10
Size: 54 1425 343 633 267 2754
Fragments arranged by size:
2754 1425 633 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3434 3793 3952 518
Size: 648 2268 359 159 2042
Fragments arranged by size:
2268 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2761 2892 3569 4717 4962 1606
Size: 1155 131 677 1148 245 2120
Fragments arranged by size:
2120 1155 1148 677 245 131
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4924
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2834 4379 1132
Size: 97 1605 1545 2229
Fragments arranged by size:
2229 1605 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5476
PciI A'CATG_T
Cuts at: 4375 4375
Size: 5476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2299 1108
Size: 1191 4285
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2650 2650
Size: 5476
PshAI GACnn'nnGTC
Cuts at: 1613 2393 1613
Size: 780 4696
PsiI TTA'TAA
Cuts at: 3046 3046
Size: 5476
PspOMI G'GGCC_C
Cuts at: 2178 2656 2178
Size: 478 4998
PstI C_TGCA'G
Cuts at: 830 2828 830
Size: 1998 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
PvuII CAG'CTG
Cuts at: 2761 2761
Size: 5476
SacI G_AGCT'C
Cuts at: 2103 2103
Size: 5476
SalI G'TCGA_C
Cuts at: 1936 2076 2818 1936
Size: 140 742 4594
Fragments arranged by size:
4594 742 140
SapI GCTCTTCn'nnn_
Cuts at: 2061 2061
Size: 5476
SbfI CC_TGCA'GG
Cuts at: 2828 2828
Size: 5476
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2671 2824 3959 4640 4831 826
Size: 219 1626 153 1135 681 191 1471
Fragments arranged by size:
1626 1471 1135 681 219 191 153
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2803 1872
Size: 931 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2018 2081 3580 4481 4743 820
Size: 197 926 75 63 1499 901 262 1553
Fragments arranged by size:
1553 1499 926 901 262 197 75 63
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 3400 5
Size: 47 3348 2081
Fragments arranged by size:
3348 2081 47
StuI AGG'CCT
Cuts at: 1517 2558 1517
Size: 1041 4435
StyI C'CwwG_G
Cuts at: 513 1065 2376 2466 2915 513
Size: 552 1311 90 449 3074
Fragments arranged by size:
3074 1311 552 449 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2424 2532 2626 3503 3520 3673 3858
Size: 1255 844 108 94 877 17 153 185
Cuts at: 3858 4303 325
Size: 445 1498
Fragments arranged by size:
1498 1255 877 844 445 185 153 108
94 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3722 5434 96
Size: 274 80 33 51 1443 1745 1712 138
Fragments arranged by size:
1745 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2399 2431 2698 2730 5436 5468 2399
Size: 32 267 32 2706 32 2407
Fragments arranged by size:
2706 2407 267 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5476
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5476
XmaI C'CCGG_G
Cuts at: 1870 2801 1870
Size: 931 4545
XmnI GAAnn'nnTTC
Cuts at: 3605 3605
Size: 5476
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3284 276
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI