Restriction map for
pFN21AB8246
(Circular) (Six-base) MAPSORT of: pFN21AB8246.seq Check: 9312 from: 1 to: 5440
pFN21AB8246 5440 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2339 2339
Size: 5440
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3250 278
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2783 1937
Size: 846 4594
Acc65I G'GTAC_C
Cuts at: 2218 2761 2218
Size: 543 4897
AclI AA'CG_TT
Cuts at: 1563 3567 3940 1563
Size: 2004 373 3063
Fragments arranged by size:
3063 2004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2401 2449 3503 4887 5155 1632
Size: 769 48 1054 1384 268 1917
Fragments arranged by size:
1917 1384 1054 769 268 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 2154 4339 5316 1203
Size: 951 2185 977 1327
Fragments arranged by size:
2185 1327 977 951
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 4169 4169
Size: 5440
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5440
AlwNI CAG_nnn'CTG
Cuts at: 2245 3183 4755 2245
Size: 938 1572 2930
Fragments arranged by size:
2930 1572 938
ApaLI G'TGCA_C
Cuts at: 2150 3497 4653 2150
Size: 1347 1156 2937
Fragments arranged by size:
2937 1347 1156
ApoI r'AATT_y
Cuts at: 1493 2385 2749 2975 3127 5236 1493
Size: 892 364 226 152 2109 1697
Fragments arranged by size:
2109 1697 892 364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3994 160
Size: 872 2962 1606
Fragments arranged by size:
2962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2765 1870
Size: 73 822 4545
Fragments arranged by size:
4545 822 73
BamHI G'GATC_C
Cuts at: 2770 3137 2770
Size: 367 5073
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2218 2761 4216 618
Size: 325 217 627 431 543 1455 1842
Fragments arranged by size:
1842 1455 627 543 431 325 217
BanII G_rGCy'C
Cuts at: 1391 2130 2759 1391
Size: 739 629 4072
Fragments arranged by size:
4072 739 629
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2407 2455 2514 2659 928
Size: 971 508 48 59 145 3709
Fragments arranged by size:
3709 971 508 145 59 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2237 2271 3631 3665 1903
Size: 34 300 34 1360 34 3678
Fragments arranged by size:
3678 1360 300 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3332 4542 3332
Size: 1210 4230
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5440
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4051 136
Size: 107 122 71 631 605 2379 1525
Fragments arranged by size:
2379 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5435 5435
Size: 5440
BlpI GC'TnA_GC
Cuts at: 2857 2857
Size: 5440
Bme1580I G_kGCm'C
Cuts at: 1790 2154 3501 4657 1790
Size: 364 1347 1156 2573
Fragments arranged by size:
2573 1347 1156 364
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3171 4129 442
Size: 416 53 83 399 24 1754 958 1753
Fragments arranged by size:
1754 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5440
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2323 2542 2323
Size: 219 5221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4100 1239
Size: 2861 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2387 3565 4430 4728 2387
Size: 1178 865 298 3099
Fragments arranged by size:
3099 1178 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2216 4103 882
Size: 617 717 1887 2219
Fragments arranged by size:
2219 1887 717 617
BsaAI yAC'GTr
Cuts at: 493 1204 2344 3231 493
Size: 711 1140 887 2702
Fragments arranged by size:
2702 1140 887 711
BsaBI GATnn'nnATC
Cuts at: 5434 5434
Size: 5440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3247 3629 4264 275
Size: 53 83 186 936 1714 382 635 1451
Fragments arranged by size:
1714 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3873 4271 4545 4692 5103 3873
Size: 398 274 147 411 4210
Fragments arranged by size:
4210 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2469 2499 1178
Size: 30 165 30 1066 30 4119
Fragments arranged by size:
4119 1066 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 1186
Size: 1126 4314
BseYI C'CCAG_C
Cuts at: 2588 2685 4643 5139 2588
Size: 97 1958 496 2889
Fragments arranged by size:
2889 1958 496 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 1163
Size: 573 564 4303
Fragments arranged by size:
4303 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2088 2890 3651 4679 1540
Size: 275 186 87 802 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 275 186 87
BsiHKAI G_wGCw'C
Cuts at: 2130 2154 2403 3501 3586 4657 2130
Size: 24 249 1098 85 1071 2913
Fragments arranged by size:
2913 1098 1071 249 85 24
BsmI GAATG_Cn'
Cuts at: 2951 3044 2951
Size: 93 5347
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2154 2403 2759 3501 3586 4657
Size: 399 340 24 249 356 742 85 1071
Cuts at: 4657 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 356 340 249 85
24
BspEI T'CCGG_A
Cuts at: 5103 5103
Size: 5440
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2339 2795 844
Size: 1495 456 3489
Fragments arranged by size:
3489 1495 456
BsrBI CCG'CTC
Cuts at: 1394 1823 2302 3327 1394
Size: 429 479 1025 3507
Fragments arranged by size:
3507 1025 479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2326 2341 2697 3935 4109 65
Size: 1183 1078 15 356 1238 174 1396
Fragments arranged by size:
1396 1238 1183 1078 356 174 15
BsrFI r'CCGG_y
Cuts at: 1700 1810 2165 4084 4271 1700
Size: 110 355 1919 187 2869
Fragments arranged by size:
2869 1919 355 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5440
BssSI C'ACGA_G
Cuts at: 3500 4512 3500
Size: 1012 4428
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5440
BstBI TT'CG_AA
Cuts at: 4244 4244
Size: 5440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5440
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2146
Size: 5440
BstYI r'GATC_y
Cuts at: 1972 2770 3137 3522 3539 4991 5077 5089 5435
Size: 798 367 385 17 1452 86 12 346
Cuts at: 5435 1972
Size: 1977
Fragments arranged by size:
1977 1452 798 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 3808
Fragments arranged by size:
3808 939 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4701
Fragments arranged by size:
4701 683 56
BtsI GCAGTG_nn'
Cuts at: 2578 2956 3750 3770 2578
Size: 378 794 20 4248
Fragments arranged by size:
4248 794 378 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5405
DraI TTT'AAA
Cuts at: 2744 2744
Size: 5440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4447 809
Size: 507 3131 1802
Fragments arranged by size:
3131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2430 2887 3776
Size: 54 1425 50 275 18 600 457 889
Cuts at: 3776 5405 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 600 457 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2887 1537
Size: 275 1075 4090
Fragments arranged by size:
4090 1075 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2535 3370 1353
Size: 1182 835 3423
Fragments arranged by size:
3423 1182 835
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4027 4401 4547 387
Size: 1064 138 2438 374 146 1280
Fragments arranged by size:
2438 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2128 2128
Size: 5440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2401 2449 3503 4100 4887 5155 1239
Size: 393 769 48 1054 597 787 268 1524
Fragments arranged by size:
1524 1054 787 769 597 393 268 48
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2561 2561
Size: 5440
FspI TGC'GCA
Cuts at: 3946 3946
Size: 5440
HaeII r_GCGC'y
Cuts at: 1678 4587 1678
Size: 2909 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2507 2539 4081 4113 4155
Size: 32 135 32 935 32 1542 32 42
Cuts at: 4155 4187 1373
Size: 32 2626
Fragments arranged by size:
2626 1542 935 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2784 3030 669
Size: 988 281 846 246 3079
Fragments arranged by size:
3079 988 846 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5440
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KpnI G_GTAC'C
Cuts at: 2222 2765 2222
Size: 543 4897
MfeI C'AATT_G
Cuts at: 2502 3039 2502
Size: 537 4903
MluI A'CGCG_T
Cuts at: 2154 5316 2154
Size: 3162 2278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4554 4738 1305
Size: 3249 184 2007
Fragments arranged by size:
3249 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3618
Fragments arranged by size:
3618 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2192 2708 3398 3757 3916 518
Size: 648 978 48 516 690 359 159 2042
Fragments arranged by size:
2042 978 690 648 516 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 2856 3533 4681 4926 1606
Size: 1250 677 1148 245 2120
Fragments arranged by size:
2120 1250 1148 677 245
NaeI GCC'GGC
Cuts at: 2167 2167
Size: 5440
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3949
Fragments arranged by size:
3949 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5440
NgoMIV G'CCGG_C
Cuts at: 2165 2165
Size: 5440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5440
NotI GC'GGCC_GC
Cuts at: 2887 2887
Size: 5440
NspI r_CATG'y
Cuts at: 1132 1229 2191 2713 2798 4343 1132
Size: 97 962 522 85 1545 2229
Fragments arranged by size:
2229 1545 962 522 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5440
PciI A'CATG_T
Cuts at: 4339 4339
Size: 5440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5440
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5440
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PstI C_TGCA'G
Cuts at: 830 2792 830
Size: 1962 3478
PvuI CG_AT'CG
Cuts at: 2001 2088 2001
Size: 87 5353
RsrII CG'GwC_CG
Cuts at: 2011 2011
Size: 5440
SacI G_AGCT'C
Cuts at: 2130 2130
Size: 5440
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SexAI A'CCwGG_T
Cuts at: 2436 2436
Size: 5440
SfcI C'TryA_G
Cuts at: 826 1045 2107 2788 3923 4604 4795 826
Size: 219 1062 681 1135 681 191 1471
Fragments arranged by size:
1471 1135 1062 681 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2767 1872
Size: 895 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2402 3544 4445 4707 820
Size: 197 926 459 1142 901 262 1553
Fragments arranged by size:
1553 1142 926 901 459 262 197
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2713 2798 1132
Size: 1581 85 3774
Fragments arranged by size:
3774 1581 85
SspI AAT'ATT
Cuts at: 5 52 3364 5
Size: 47 3312 2081
Fragments arranged by size:
3312 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5440
StyI C'CwwG_G
Cuts at: 513 1065 2004 2313 2349 2879 513
Size: 552 939 309 36 530 3074
Fragments arranged by size:
3074 939 552 530 309 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2373 3467 3484 3637 3822 4267 325
Size: 1255 793 1094 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1094 793 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3686 5398 96
Size: 274 80 33 51 1443 1709 1712 138
Fragments arranged by size:
1712 1709 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2469 2501 5400 5432 2469
Size: 32 2899 32 2477
Fragments arranged by size:
2899 2477 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5440
XbaI T'CTAG_A
Cuts at: 2776 2776
Size: 5440
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5440
XmaI C'CCGG_G
Cuts at: 1870 2765 1870
Size: 895 4545
XmnI GAAnn'nnTTC
Cuts at: 2385 3569 2385
Size: 1184 4256
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3248 276
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI RsrII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI