Restriction map for
pFN21AB8244
(Circular) (Six-base) MAPSORT of: pFN21AB8244.seq Check: 8208 from: 1 to: 5707
pFN21AB8244 5707 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 3770 5154 5422 1632
Size: 581 1557 1384 268 1917
Fragments arranged by size:
1917 1557 1384 581 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 3450 5022 3450
Size: 1572 4135
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 3016 3242 3394 5503 1493
Size: 1523 226 152 2109 1697
Fragments arranged by size:
2109 1697 1523 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2319 3032 1870
Size: 73 376 713 4545
Fragments arranged by size:
4545 713 376 73
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2555 3028 4483 618
Size: 325 217 627 768 473 1455 1842
Fragments arranged by size:
1842 1455 768 627 473 325 217
BanII G_rGCy'C
Cuts at: 1391 3026 1391
Size: 1635 4072
BbeI G_GCGC'C
Cuts at: 2559 2559
Size: 5707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2901 928
Size: 971 1002 3734
Fragments arranged by size:
3734 1002 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 2274 1548
Size: 726 4981
BfrBI ATG'CAT
Cuts at: 2206 2206
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2427 4318
Size: 107 122 71 631 605 337 418 1891
Cuts at: 4318 136
Size: 1525
Fragments arranged by size:
1891 1525 631 605 418 337 122 107
71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 2312 3768 4924 1790
Size: 522 1456 1156 2573
Fragments arranged by size:
2573 1456 1156 522
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2749 3438 4396
Size: 416 53 83 399 24 1332 689 958
Cuts at: 4396 442
Size: 1753
Fragments arranged by size:
1753 1332 958 689 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4367 1239
Size: 3128 2579
Bpu10I CC'TnA_GC
Cuts at: 2331 2331
Size: 5707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2738 3832 4697 4995 2738
Size: 1094 865 298 3450
Fragments arranged by size:
3450 1094 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4370 882
Size: 617 2871 2219
Fragments arranged by size:
2871 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2556 3514 3896
Size: 53 83 186 936 479 544 958 382
Cuts at: 3896 4531 275
Size: 635 1451
Fragments arranged by size:
1451 958 936 635 544 479 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5707
BseYI C'CCAG_C
Cuts at: 2838 2842 4910 5406 2838
Size: 4 2068 496 3139
Fragments arranged by size:
3139 2068 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3768 3853 4924 3768
Size: 85 1071 4551
Fragments arranged by size:
4551 1071 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2312 3026 3768 3853 4924 1391
Size: 399 522 714 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 714 522 399 85
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3062 844
Size: 2218 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2022 3594 1394
Size: 429 199 1572 3507
Fragments arranged by size:
3507 1572 429 199
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4351 4538 1700
Size: 110 2541 187 2869
Fragments arranged by size:
2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 2650 96
Size: 2554 3153
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 3037 3404 3789 3806 5258 5344 5356 5702
Size: 1065 367 385 17 1452 86 12 346
Cuts at: 5702 1972
Size: 1977
Fragments arranged by size:
1977 1452 1065 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2950 513
Size: 552 939 946 3270
Fragments arranged by size:
3270 946 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 2511 2653 3223 4017 4037 2511
Size: 142 570 794 20 4181
Fragments arranged by size:
4181 794 570 142 20
ClaI AT'CG_AT
Cuts at: 1554 2079 1554
Size: 525 5182
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2386 3154
Size: 54 1425 50 275 18 219 337 768
Cuts at: 3154 4043 5672 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 768 337 275 219 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3637 1353
Size: 2284 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 3770 4367 5154 5422 1239
Size: 393 581 1557 597 787 268 1524
Fragments arranged by size:
1557 1524 787 597 581 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2595 2595
Size: 5707
EcoO109I rG'GnC_Cy
Cuts at: 2600 2600
Size: 5707
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2567 2918 2567
Size: 351 5356
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 2559 4854 1678
Size: 881 2295 2531
Fragments arranged by size:
2531 2295 881
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2131 2163 2461 2493 2873
Size: 32 135 32 559 32 298 32 380
Cuts at: 2873 2905 4348 4380 4422 4454 1373
Size: 32 1443 32 42 32 2626
Fragments arranged by size:
2626 1443 559 380 298 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KasI G'GCGC_C
Cuts at: 2555 2555
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2543 4821 5005 1305
Size: 1238 2278 184 2007
Fragments arranged by size:
2278 2007 1238 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2051 10
Size: 54 1425 343 219 3666
Fragments arranged by size:
3666 1425 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3665 4024 4183 518
Size: 648 2499 359 159 2042
Fragments arranged by size:
2499 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2842 2925 3123 3800 4948 5193 1606
Size: 1236 83 198 677 1148 245 2120
Fragments arranged by size:
2120 1236 1148 677 245 198 83
NarI GG'CG_CC
Cuts at: 2556 2556
Size: 5707
NcoI C'CATG_G
Cuts at: 513 1065 2004 2950 513
Size: 552 939 946 3270
Fragments arranged by size:
3270 946 939 552
NdeI CA'TA_TG
Cuts at: 387 2344 387
Size: 1957 3750
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NsiI A_TGCA'T
Cuts at: 2208 2208
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 2062 2210 3065 4610 1132
Size: 97 833 148 855 1545 2229
Fragments arranged by size:
2229 1545 855 833 148 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PfoI T'CCnGG_A
Cuts at: 2015 2487 2015
Size: 472 5235
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2600 2600
Size: 5707
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PstI C_TGCA'G
Cuts at: 830 3059 830
Size: 2229 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2842 2925 2842
Size: 83 5624
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 2165 2464 3955 5667 2165
Size: 299 1491 1712 2205
Fragments arranged by size:
2205 1712 1491 299
SfcI C'TryA_G
Cuts at: 826 1045 3055 4190 4871 5062 826
Size: 219 2010 1135 681 191 1471
Fragments arranged by size:
2010 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2557 2557
Size: 5707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2717 3811 4712 4974 820
Size: 197 926 774 1094 901 262 1553
Fragments arranged by size:
1553 1094 926 901 774 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 2210 3065 1132
Size: 1078 855 3774
Fragments arranged by size:
3774 1078 855
SspI AAT'ATT
Cuts at: 5 52 2666 3631 5
Size: 47 2614 965 2081
Fragments arranged by size:
2614 2081 965 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2004 2950 3146 513
Size: 552 939 946 196 3074
Fragments arranged by size:
3074 946 939 552 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2731 2898 3734 3751 3904 4089 4534
Size: 1255 1151 167 836 17 153 185 445
Cuts at: 4534 325
Size: 1498
Fragments arranged by size:
1498 1255 1151 836 445 185 167 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 2462 2650
Size: 274 80 33 51 1443 186 299 188
Cuts at: 2650 3953 5665 96
Size: 1303 1712 138
Fragments arranged by size:
1712 1443 1303 299 274 188 186 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5667 5699 5667
Size: 32 5675
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2278 2429 2278
Size: 151 5556
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 3836 3836
Size: 5707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI