Restriction map for
pFN21AB8237
(Circular) (Six-base) MAPSORT of: pFN21AB8237.seq Check: 6858 from: 1 to: 5482
pFN21AB8237 5482 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3292 278
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2825 1937
Size: 888 4594
Acc65I G'GTAC_C
Cuts at: 2803 2803
Size: 5482
AclI AA'CG_TT
Cuts at: 1563 3609 3982 1563
Size: 2046 373 3063
Fragments arranged by size:
3063 2046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2097 2222 2339 3545 4929 5197 1632
Size: 465 125 117 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1206 465 268 125 117
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5285
AflIII A'CryG_T
Cuts at: 1203 4381 5358 1203
Size: 3178 977 1327
Fragments arranged by size:
3178 1327 977
AgeI A'CCGG_T
Cuts at: 4313 4313
Size: 5482
AhdI GACnn_n'nnGTC
Cuts at: 2275 4211 2275
Size: 1936 3546
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2070 2780 1184
Size: 886 710 3886
Fragments arranged by size:
3886 886 710
AlwNI CAG_nnn'CTG
Cuts at: 2114 3225 4797 2114
Size: 1111 1572 2799
Fragments arranged by size:
2799 1572 1111
ApaLI G'TGCA_C
Cuts at: 3539 4695 3539
Size: 1156 4326
ApoI r'AATT_y
Cuts at: 1493 2791 3017 3169 5278 1493
Size: 1298 226 152 2109 1697
Fragments arranged by size:
2109 1697 1298 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4036 160
Size: 872 3004 1606
Fragments arranged by size:
3004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
AvaI C'yCGr_G
Cuts at: 1870 1943 2634 2807 1870
Size: 73 691 173 4545
Fragments arranged by size:
4545 691 173 73
BamHI G'GATC_C
Cuts at: 2812 3179 2812
Size: 367 5115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2441 2764 2803 4258 618
Size: 325 217 627 654 323 39 1455 1842
Fragments arranged by size:
1842 1455 654 627 325 323 217 39
BanII G_rGCy'C
Cuts at: 1391 2801 1391
Size: 1410 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2034 2085 928
Size: 971 135 51 4325
Fragments arranged by size:
4325 971 135 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3673 3707 1903
Size: 34 1736 34 3678
Fragments arranged by size:
3678 1736 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2696 3374 4584 2696
Size: 678 1210 3594
Fragments arranged by size:
3594 1210 678
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2630 4093 136
Size: 107 122 71 631 605 958 1463 1525
Fragments arranged by size:
1525 1463 958 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5477 5477
Size: 5482
BlpI GC'TnA_GC
Cuts at: 2899 2899
Size: 5482
Bme1580I G_kGCm'C
Cuts at: 1790 2769 3543 4699 1790
Size: 979 774 1156 2573
Fragments arranged by size:
2573 1156 979 774
BmgBI CAC'GTC
Cuts at: 1318 2542 1318
Size: 1224 4258
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3213 4171 442
Size: 416 53 83 399 24 1796 958 1753
Fragments arranged by size:
1796 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 2552 4142 1239
Size: 1214 99 1590 2579
Fragments arranged by size:
2579 1590 1214 99
Bpu10I CC'TnA_GC
Cuts at: 2145 2145
Size: 5482
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2726 3607 4472 4770 2726
Size: 881 865 298 3438
Fragments arranged by size:
3438 881 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2408 4145 882
Size: 617 909 1737 2219
Fragments arranged by size:
2219 1737 909 617
BsaAI yAC'GTr
Cuts at: 493 1204 2608 3273 493
Size: 711 1404 665 2702
Fragments arranged by size:
2702 1404 711 665
BsaBI GATnn'nnATC
Cuts at: 5476 5476
Size: 5482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2576 3289 3671 4306
Size: 53 83 186 936 1043 713 382 635
Cuts at: 4306 275
Size: 1451
Fragments arranged by size:
1451 1043 936 713 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2164 2706 3915 4313 4587 4734 5145 2164
Size: 542 1209 398 274 147 411 2501
Fragments arranged by size:
2501 1209 542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5257
Fragments arranged by size:
5257 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 1186
Size: 919 4563
BseYI C'CCAG_C
Cuts at: 2016 2043 4685 5181 2016
Size: 27 2642 496 2317
Fragments arranged by size:
2642 2317 496 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2122 1163
Size: 573 386 4523
Fragments arranged by size:
4523 573 386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2932 3693 4721 1540
Size: 275 186 931 761 1028 2301
Fragments arranged by size:
2301 1028 931 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3543 3628 4699 3543
Size: 85 1071 4326
Fragments arranged by size:
4326 1071 85
BsmI GAATG_Cn'
Cuts at: 2993 3086 2993
Size: 93 5389
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2769 2801 3543 3628 4699 1391
Size: 399 979 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 979 742 399 85 32
BspEI T'CCGG_A
Cuts at: 5145 5145
Size: 5482
BspHI T'CATG_A
Cuts at: 3371 5101 3371
Size: 1730 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2837 844
Size: 1993 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3369 1394
Size: 429 1546 3507
Fragments arranged by size:
3507 1546 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3977 4151 65
Size: 1183 2729 174 1396
Fragments arranged by size:
2729 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2226 2257 4126 4313 1700
Size: 110 416 31 1869 187 2869
Fragments arranged by size:
2869 1869 416 187 110 31
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5482
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5482
BssSI C'ACGA_G
Cuts at: 3542 4554 3542
Size: 1012 4470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5482
BstBI TT'CG_AA
Cuts at: 4286 4286
Size: 5482
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5482
BstYI r'GATC_y
Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477
Size: 840 367 385 17 1452 86 12 346
Cuts at: 5477 1972
Size: 1977
Fragments arranged by size:
1977 1452 840 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2778 2778
Size: 5482
BtgI C'CryG_G
Cuts at: 513 1065 2004 2691 513
Size: 552 939 687 3304
Fragments arranged by size:
3304 939 687 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4743
Fragments arranged by size:
4743 683 56
BtsI GCAGTG_nn'
Cuts at: 2579 2998 3792 3812 2579
Size: 419 794 20 4249
Fragments arranged by size:
4249 794 419 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5447
DraI TTT'AAA
Cuts at: 2786 2786
Size: 5482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2117 2348 4489 809
Size: 507 801 231 2141 1802
Fragments arranged by size:
2141 1802 801 507 231
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2490 2929
Size: 54 1425 50 275 18 429 231 439
Cuts at: 2929 3818 5447 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 439 429 275 231 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2929 1537
Size: 275 1117 4090
Fragments arranged by size:
4090 1117 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2753 3412 1353
Size: 1400 659 3423
Fragments arranged by size:
3423 1400 659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2386 4069 4443 4589 387
Size: 1064 138 797 1683 374 146 1280
Fragments arranged by size:
1683 1280 1064 797 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2222 2339 2453 2552 3545 4142
Size: 393 465 125 117 114 99 993 597
Cuts at: 4142 4929 5197 1239
Size: 787 268 1524
Fragments arranged by size:
1524 993 787 597 465 393 268 125
117 114 99
EcoO109I rG'GnC_Cy
Cuts at: 2071 2071
Size: 5482
EcoRI G'AATT_C
Cuts at: 2791 3169 2791
Size: 378 5104
FspI TGC'GCA
Cuts at: 3988 3988
Size: 5482
HaeII r_GCGC'y
Cuts at: 1678 2481 2643 4629 1678
Size: 803 162 1986 2531
Fragments arranged by size:
2531 1986 803 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 4123 4155 4197
Size: 32 135 32 689 32 1830 32 42
Cuts at: 4197 4229 1373
Size: 32 2626
Fragments arranged by size:
2626 1830 689 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2353 2826 3072 669
Size: 988 281 415 473 246 3079
Fragments arranged by size:
3079 988 473 415 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5482
HpaI GTT'AAC
Cuts at: 3072 3072
Size: 5482
KpnI G_GTAC'C
Cuts at: 2807 2807
Size: 5482
MfeI C'AATT_G
Cuts at: 3081 3081
Size: 5482
MluI A'CGCG_T
Cuts at: 5358 5358
Size: 5482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4596 4780 1305
Size: 3291 184 2007
Fragments arranged by size:
3291 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 10
Size: 54 1425 343 660 3000
Fragments arranged by size:
3000 1425 660 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 3440 3799 3958 518
Size: 648 1512 762 359 159 2042
Fragments arranged by size:
2042 1512 762 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2317 2771 2898 3575 4723 4968 1606
Size: 711 454 127 677 1148 245 2120
Fragments arranged by size:
2120 1148 711 677 454 245 127
NaeI GCC'GGC
Cuts at: 2259 2259
Size: 5482
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5482
NgoMIV G'CCGG_C
Cuts at: 2257 2257
Size: 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5482
NotI GC'GGCC_GC
Cuts at: 2929 2929
Size: 5482
NspI r_CATG'y
Cuts at: 1132 1229 2840 4385 1132
Size: 97 1611 1545 2229
Fragments arranged by size:
2229 1611 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2698 1348
Size: 1350 4132
PciI A'CATG_T
Cuts at: 4381 4381
Size: 5482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2458 1108
Size: 1350 4132
PmeI GTTT'AAAC
Cuts at: 2786 2786
Size: 5482
PmlI CAC'GTG
Cuts at: 2608 2608
Size: 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 5064 5096 3551
Size: 32 1481 32 3937
Fragments arranged by size:
3937 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2071
Size: 5482
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5482
PsiI TTA'TAA
Cuts at: 3052 3052
Size: 5482
PstI C_TGCA'G
Cuts at: 830 2026 2101 2834 830
Size: 1196 75 733 3478
Fragments arranged by size:
3478 1196 733 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5482
PvuII CAG'CTG
Cuts at: 2317 2317
Size: 5482
SalI G'TCGA_C
Cuts at: 1936 2824 1936
Size: 888 4594
SanDI GG'GwC_CC
Cuts at: 2071 2071
Size: 5482
SapI GCTCTTCn'nnn_
Cuts at: 2753 2753
Size: 5482
SbfI CC_TGCA'GG
Cuts at: 2834 2834
Size: 5482
ScaI AGT'ACT
Cuts at: 3730 5442 3730
Size: 1712 3770
SexAI A'CCwGG_T
Cuts at: 2760 2760
Size: 5482
SfcI C'TryA_G
Cuts at: 826 1045 2022 2097 2830 3965 4646 4837 826
Size: 219 977 75 733 1135 681 191 1471
Fragments arranged by size:
1471 1135 977 733 681 219 191 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 2630 2630
Size: 5482
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5482
SmaI CCC'GGG
Cuts at: 1872 2809 1872
Size: 937 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2741 3586 4487 4749 820
Size: 197 926 798 845 901 262 1553
Fragments arranged by size:
1553 926 901 845 798 262 197
SnaBI TAC'GTA
Cuts at: 493 3273 493
Size: 2780 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5482
SphI G_CATG'C
Cuts at: 1132 2840 1132
Size: 1708 3774
SspI AAT'ATT
Cuts at: 5 52 3406 5
Size: 47 3354 2081
Fragments arranged by size:
3354 2081 47
StuI AGG'CCT
Cuts at: 1517 2624 1517
Size: 1107 4375
StyI C'CwwG_G
Cuts at: 513 1065 2004 2921 513
Size: 552 939 917 3074
Fragments arranged by size:
3074 939 917 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3509 3526 3679 3864 4309 325
Size: 1255 1929 17 153 185 445 1498
Fragments arranged by size:
1929 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3728 5440 96
Size: 274 80 33 51 1443 1751 1712 138
Fragments arranged by size:
1751 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5442 5474 5442
Size: 32 5450
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5482
XbaI T'CTAG_A
Cuts at: 2818 2818
Size: 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5482
XmaI C'CCGG_G
Cuts at: 1870 2807 1870
Size: 937 4545
XmnI GAAnn'nnTTC
Cuts at: 3611 3611
Size: 5482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3290 276
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII
SacI SacII SfoI SrfI SwaI XcmI