Restriction map for
pFN21AB8235
(Circular) (Six-base) MAPSORT of: pFN21AB8235.seq Check: 382 from: 1 to: 5365
pFN21AB8235 5365 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2626 2626
Size: 5365
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2114 3175 278
Size: 53 83 186 936 578 1061 2468
Fragments arranged by size:
2468 1061 936 578 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2708 1937
Size: 771 4594
Acc65I G'GTAC_C
Cuts at: 2253 2686 2253
Size: 433 4932
AclI AA'CG_TT
Cuts at: 1563 3492 3865 1563
Size: 1929 373 3063
Fragments arranged by size:
3063 1929 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3428 4812 5080 1632
Size: 1796 1384 268 1917
Fragments arranged by size:
1917 1796 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5168
AflIII A'CryG_T
Cuts at: 1203 2067 4264 5241 1203
Size: 864 2197 977 1327
Fragments arranged by size:
2197 1327 977 864
AgeI A'CCGG_T
Cuts at: 4196 4196
Size: 5365
AhdI GACnn_n'nnGTC
Cuts at: 4094 4094
Size: 5365
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5365
AlwNI CAG_nnn'CTG
Cuts at: 2395 3108 4680 2395
Size: 713 1572 3080
Fragments arranged by size:
3080 1572 713
ApaI G_GGCC'C
Cuts at: 2618 2618
Size: 5365
ApaLI G'TGCA_C
Cuts at: 3422 4578 3422
Size: 1156 4209
ApoI r'AATT_y
Cuts at: 1493 2674 2900 3052 5161 1493
Size: 1181 226 152 2109 1697
Fragments arranged by size:
2109 1697 1181 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3919 160
Size: 872 2887 1606
Fragments arranged by size:
2887 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
AvaI C'yCGr_G
Cuts at: 1870 1943 2690 1870
Size: 73 747 4545
Fragments arranged by size:
4545 747 73
BamHI G'GATC_C
Cuts at: 2695 3062 2695
Size: 367 4998
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2253 2603 2638 2686 4141
Size: 325 217 627 466 350 35 48 1455
Cuts at: 4141 618
Size: 1842
Fragments arranged by size:
1842 1455 627 466 350 325 217 48
35
BanII G_rGCy'C
Cuts at: 1391 2618 2684 1391
Size: 1227 66 4072
Fragments arranged by size:
4072 1227 66
BbeI G_GCGC'C
Cuts at: 2607 2607
Size: 5365
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3556 3590 1903
Size: 34 1619 34 3678
Fragments arranged by size:
3678 1619 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3257 4467 3257
Size: 1210 4155
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3976 136
Size: 107 122 71 631 605 2304 1525
Fragments arranged by size:
2304 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2226 5360 2226
Size: 3134 2231
BlpI GC'TnA_GC
Cuts at: 2782 2782
Size: 5365
Bme1580I G_kGCm'C
Cuts at: 1790 2618 3426 4582 1790
Size: 828 808 1156 2573
Fragments arranged by size:
2573 1156 828 808
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5365
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3096 4054 442
Size: 416 53 83 399 24 1679 958 1753
Fragments arranged by size:
1753 1679 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2465 1056
Size: 1409 3956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4025 1239
Size: 2786 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3490 4355 4653 3490
Size: 865 298 4202
Fragments arranged by size:
4202 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4028 882
Size: 617 2529 2219
Fragments arranged by size:
2529 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3156 493
Size: 711 1952 2702
Fragments arranged by size:
2702 1952 711
BsaBI GATnn'nnATC
Cuts at: 2081 5359 2081
Size: 3278 2087
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2111 2604 3172 3554
Size: 53 83 186 936 578 493 568 382
Cuts at: 3554 4189 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 578 568 493 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2256 3798 4196 4470 4617 5028 2256
Size: 1542 398 274 147 411 2593
Fragments arranged by size:
2593 1542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5140
Fragments arranged by size:
5140 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5365
BseYI C'CCAG_C
Cuts at: 4568 5064 4568
Size: 496 4869
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 1163
Size: 573 627 4165
Fragments arranged by size:
4165 627 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2815 3576 4604 1540
Size: 275 186 814 761 1028 2301
Fragments arranged by size:
2301 1028 814 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3426 3511 4582 3426
Size: 85 1071 4209
Fragments arranged by size:
4209 1071 85
BsmI GAATG_Cn'
Cuts at: 2876 2969 2876
Size: 93 5272
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2618 2684 3426 3511 4582 1391
Size: 399 828 66 742 85 1071 2174
Fragments arranged by size:
2174 1071 828 742 399 85 66
BspEI T'CCGG_A
Cuts at: 5028 5028
Size: 5365
BspHI T'CATG_A
Cuts at: 3254 4984 3254
Size: 1730 3635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2626 2720 844
Size: 1782 94 3489
Fragments arranged by size:
3489 1782 94
BsrBI CCG'CTC
Cuts at: 1394 1823 2251 2431 3252 1394
Size: 429 428 180 821 3507
Fragments arranged by size:
3507 821 429 428 180
BsrDI GCAATG_nn'
Cuts at: 65 1248 3860 4034 65
Size: 1183 2612 174 1396
Fragments arranged by size:
2612 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2454 4009 4196 1700
Size: 110 644 1555 187 2869
Fragments arranged by size:
2869 1555 644 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5365
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5365
BssSI C'ACGA_G
Cuts at: 3425 4437 3425
Size: 1012 4353
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5365
BstBI TT'CG_AA
Cuts at: 4169 4169
Size: 5365
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5365
BstYI r'GATC_y
Cuts at: 1972 2226 2695 3062 3447 3464 4916 5002 5014
Size: 254 469 367 385 17 1452 86 12
Cuts at: 5014 5360 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 469 385 367 346 254 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2166 513
Size: 552 939 162 3712
Fragments arranged by size:
3712 939 552 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4626
Fragments arranged by size:
4626 683 56
BtsI GCAGTG_nn'
Cuts at: 2346 2881 3675 3695 2346
Size: 535 794 20 4016
Fragments arranged by size:
4016 794 535 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5365
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5330
DraI TTT'AAA
Cuts at: 2669 2669
Size: 5365
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2183 2594 4372 809
Size: 507 867 411 1778 1802
Fragments arranged by size:
1802 1778 867 507 411
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2452 2812 3701
Size: 54 1425 50 275 18 622 360 889
Cuts at: 3701 5330 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 622 360 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2812 1537
Size: 275 1000 4090
Fragments arranged by size:
4090 1000 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 3295 1353
Size: 773 1169 3423
Fragments arranged by size:
3423 1169 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2002 2600 3952 4326 4472 387
Size: 1064 138 413 598 1352 374 146 1280
Fragments arranged by size:
1352 1280 1064 598 413 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3428 4025 4812 5080 1239
Size: 393 1796 597 787 268 1524
Fragments arranged by size:
1796 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2614 2614
Size: 5365
EcoRI G'AATT_C
Cuts at: 2674 3052 2674
Size: 378 4987
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2545 2131
Size: 414 4951
FspI TGC'GCA
Cuts at: 3871 3871
Size: 5365
HaeII r_GCGC'y
Cuts at: 1678 2289 2502 2539 2607 4512 1678
Size: 611 213 37 68 1905 2531
Fragments arranged by size:
2531 1905 611 213 68 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2182 2214 2549 2581 4006
Size: 32 135 32 610 32 335 32 1425
Cuts at: 4006 4038 4080 4112 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1425 610 335 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2709 2955 669
Size: 988 281 771 246 3079
Fragments arranged by size:
3079 988 771 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5365
HpaI GTT'AAC
Cuts at: 2955 2955
Size: 5365
KasI G'GCGC_C
Cuts at: 2603 2603
Size: 5365
KpnI G_GTAC'C
Cuts at: 2257 2690 2257
Size: 433 4932
MfeI C'AATT_G
Cuts at: 2964 2964
Size: 5365
MluI A'CGCG_T
Cuts at: 5241 5241
Size: 5365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2556 4479 4663 1305
Size: 1251 1923 184 2007
Fragments arranged by size:
2007 1923 1251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3543
Fragments arranged by size:
3543 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 3323 3682 3841 518
Size: 648 1137 1020 359 159 2042
Fragments arranged by size:
2042 1137 1020 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2781 3458 4606 4851 1606
Size: 1175 677 1148 245 2120
Fragments arranged by size:
2120 1175 1148 677 245
NaeI GCC'GGC
Cuts at: 2456 2456
Size: 5365
NarI GG'CG_CC
Cuts at: 2604 2604
Size: 5365
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3874
Fragments arranged by size:
3874 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5365
NgoMIV G'CCGG_C
Cuts at: 2454 2454
Size: 5365
NheI G'CTAG_C
Cuts at: 1052 2461 1052
Size: 1409 3956
NotI GC'GGCC_GC
Cuts at: 2812 2812
Size: 5365
NspI r_CATG'y
Cuts at: 1132 1229 2071 2723 4268 1132
Size: 97 842 652 1545 2229
Fragments arranged by size:
2229 1545 842 652 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5365
PciI A'CATG_T
Cuts at: 2067 4264 2067
Size: 2197 3168
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5365
PmeI GTTT'AAAC
Cuts at: 2669 2669
Size: 5365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 4947 4979 3434
Size: 32 1481 32 3820
Fragments arranged by size:
3820 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5365
PsiI TTA'TAA
Cuts at: 2935 2935
Size: 5365
PspOMI G'GGCC_C
Cuts at: 2614 2614
Size: 5365
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2048 2080 2048
Size: 32 5333
PstI C_TGCA'G
Cuts at: 830 2226 2717 830
Size: 1396 491 3478
Fragments arranged by size:
3478 1396 491
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5365
SalI G'TCGA_C
Cuts at: 1936 2707 1936
Size: 771 4594
SbfI CC_TGCA'GG
Cuts at: 2717 2717
Size: 5365
ScaI AGT'ACT
Cuts at: 3613 5325 3613
Size: 1712 3653
SfcI C'TryA_G
Cuts at: 826 1045 2222 2713 3848 4529 4720 826
Size: 219 1177 491 1135 681 191 1471
Fragments arranged by size:
1471 1177 1135 681 491 219 191
SfoI GGC'GCC
Cuts at: 2605 2605
Size: 5365
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5365
SmaI CCC'GGG
Cuts at: 1872 2692 1872
Size: 820 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3469 4370 4632 820
Size: 197 926 1526 901 262 1553
Fragments arranged by size:
1553 1526 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3156 493
Size: 2663 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5365
SphI G_CATG'C
Cuts at: 1132 2723 1132
Size: 1591 3774
SspI AAT'ATT
Cuts at: 5 52 3289 5
Size: 47 3237 2081
Fragments arranged by size:
3237 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5365
StyI C'CwwG_G
Cuts at: 513 1065 2004 2473 2502 2804 513
Size: 552 939 469 29 302 3074
Fragments arranged by size:
3074 939 552 469 302 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2372 3392 3409 3562 3747 4192 325
Size: 1255 792 1020 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1020 792 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3611 5323 96
Size: 274 80 33 51 1443 1634 1712 138
Fragments arranged by size:
1712 1634 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5325 5357 5325
Size: 32 5333
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5365
XbaI T'CTAG_A
Cuts at: 2701 2701
Size: 5365
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5365
XmaI C'CCGG_G
Cuts at: 1870 2690 1870
Size: 820 4545
XmnI GAAnn'nnTTC
Cuts at: 3494 3494
Size: 5365
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2112 3173 276
Size: 53 83 186 936 578 1061 2468
Fragments arranged by size:
2468 1061 936 578 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI