Restriction map for
pFN21AB8230
(Circular) (Six-base) MAPSORT of: pFN21AB8230.seq Check: 460 from: 1 to: 5743
pFN21AB8230 5743 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2422 2422
Size: 5743
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3015 3086 1937
Size: 1078 71 4594
Fragments arranged by size:
4594 1078 71
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2276 2976 3806 5190 5458 1632
Size: 644 700 830 1384 268 1917
Fragments arranged by size:
1917 1384 830 700 644 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 3486 5058 3486
Size: 1572 4171
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 3052 3278 3430 5539 1493
Size: 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2154 4297 160
Size: 872 1122 2143 1606
Fragments arranged by size:
2143 1606 1122 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2697 3068 1870
Size: 73 81 673 371 4545
Fragments arranged by size:
4545 673 371 81 73
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 3064 4519 618
Size: 325 217 627 1199 78 1455 1842
Fragments arranged by size:
1842 1455 1199 627 325 217 78
BanII G_rGCy'C
Cuts at: 1391 3062 1391
Size: 1671 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4772
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4354 136
Size: 107 122 71 631 605 2682 1525
Fragments arranged by size:
2682 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5738 5738
Size: 5743
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 3804 4960 1790
Size: 2014 1156 2573
Fragments arranged by size:
2573 2014 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2769 3474 4432
Size: 416 53 83 399 24 1352 705 958
Cuts at: 4432 442
Size: 1753
Fragments arranged by size:
1753 1352 958 705 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2744 2062
Size: 682 5061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 4403 1239
Size: 1253 1911 2579
Fragments arranged by size:
2579 1911 1253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2959 3868 4733 5031 2959
Size: 909 865 298 3671
Fragments arranged by size:
3671 909 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3015 4406 882
Size: 617 1516 1391 2219
Fragments arranged by size:
2219 1516 1391 617
BsaAI yAC'GTr
Cuts at: 493 1204 2243 3534 493
Size: 711 1039 1291 2702
Fragments arranged by size:
2702 1291 1039 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2669 3012 3550 3932
Size: 53 83 186 936 1136 343 538 382
Cuts at: 3932 4567 275
Size: 635 1451
Fragments arranged by size:
1451 1136 936 635 538 382 343 186
83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2640 2659 2670
Size: 30 165 30 597 30 610 19 11
Cuts at: 2670 2689 1178
Size: 19 4232
Fragments arranged by size:
4232 610 597 165 30 30 30 19
19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2640 2697 2760 2948 1186
Size: 838 616 57 63 188 3981
Fragments arranged by size:
3981 838 616 188 63 57
BseYI C'CCAG_C
Cuts at: 2170 2602 2884 4946 5442 2170
Size: 432 282 2062 496 2471
Fragments arranged by size:
2471 2062 496 432 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3804 3889 4960 3804
Size: 85 1071 4587
Fragments arranged by size:
4587 1071 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3062 3804 3889 4960 1391
Size: 399 1272 742 85 1071 2174
Fragments arranged by size:
2174 1272 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2028 2422 3098 844
Size: 1184 394 676 3489
Fragments arranged by size:
3489 1184 676 394
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 2661 3803 4815 2661
Size: 1142 1012 3589
Fragments arranged by size:
3589 1142 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2074 794
Size: 1280 4463
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstXI CCAn_nnnn'nTGG
Cuts at: 3032 3032
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 3073 3440 3825 3842 5294 5380 5392 5738
Size: 1101 367 385 17 1452 86 12 346
Cuts at: 5738 1972
Size: 1977
Fragments arranged by size:
1977 1452 1101 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 2832 3259 4053 4073 2832
Size: 427 794 20 4502
Fragments arranged by size:
4502 794 427 20
ClaI AT'CG_AT
Cuts at: 1554 2142 1554
Size: 588 5155
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2945 2980 529
Size: 35 2381 35 3292
Fragments arranged by size:
3292 2381 35 35
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2287 4750 809
Size: 507 971 2463 1802
Fragments arranged by size:
2463 1802 971 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2639 3190 4079
Size: 54 1425 50 275 18 809 551 889
Cuts at: 4079 5708 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 809 551 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2328 2713 2955 3673 1353
Size: 975 385 242 718 3423
Fragments arranged by size:
3423 975 718 385 242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 4330 4704 4850 387
Size: 1064 138 462 2279 374 146 1280
Fragments arranged by size:
2279 1280 1064 462 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2492 2976 3806 4403 5190 5458
Size: 393 644 216 484 830 597 787 268
Cuts at: 5458 1239
Size: 1524
Fragments arranged by size:
1524 830 787 644 597 484 393 268
216
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 5743
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 4890 1678
Size: 3212 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2659 2691 2910 2942 4384
Size: 32 135 32 1087 32 219 32 1442
Cuts at: 4384 4416 4458 4490 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1442 1087 219 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2517 3087 3333 669
Size: 988 281 579 570 246 3079
Fragments arranged by size:
3079 988 579 570 281 246
HindIII A'AGCT_T
Cuts at: 748 2421 748
Size: 1673 4070
HpaI GTT'AAC
Cuts at: 2517 3333 2517
Size: 816 4927
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2626 4857 5041 1305
Size: 1321 2231 184 2007
Fragments arranged by size:
2231 2007 1321 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3921
Fragments arranged by size:
3921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2373 2399 3701 4060 4219 518
Size: 648 978 229 26 1302 359 159 2042
Fragments arranged by size:
2042 1302 978 648 359 229 159 26
MspA1I CmG'CkG
Cuts at: 1606 3159 3836 4984 5229 1606
Size: 1553 677 1148 245 2120
Fragments arranged by size:
2120 1553 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 3031 387
Size: 2644 3099
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 2444 3101 4646 1132
Size: 97 1215 657 1545 2229
Fragments arranged by size:
2229 1545 1215 657 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5743
PfoI T'CCnGG_A
Cuts at: 2508 2508
Size: 5743
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PmlI CAC'GTG
Cuts at: 2243 2243
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 2481 3313 2481
Size: 832 4911
PstI C_TGCA'G
Cuts at: 830 2419 3095 830
Size: 1589 676 3478
Fragments arranged by size:
3478 1589 676
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 2363 3991 5703 2363
Size: 1628 1712 2403
Fragments arranged by size:
2403 1712 1628
SfcI C'TryA_G
Cuts at: 826 1045 2415 2875 3016 3091 4226 4907 5098
Size: 219 1370 460 141 75 1135 681 191
Cuts at: 5098 826
Size: 1471
Fragments arranged by size:
1471 1370 1135 681 460 219 191 141
75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2974 3847 4748 5010 820
Size: 197 926 1031 873 901 262 1553
Fragments arranged by size:
1553 1031 926 901 873 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2004 2296 2325 3182 513
Size: 552 939 292 29 857 3074
Fragments arranged by size:
3074 939 857 552 292 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 2361 3989
Size: 274 80 33 51 1443 159 225 1628
Cuts at: 3989 5701 96
Size: 1712 138
Fragments arranged by size:
1712 1628 1443 274 225 159 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2166 2198 5703 5735 2166
Size: 32 3505 32 2174
Fragments arranged by size:
3505 2174 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 2103 2363 3872 2103
Size: 260 1509 3974
Fragments arranged by size:
3974 1509 260
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI