Restriction map for
pFN21AB8214
(Circular) (Six-base) MAPSORT of: pFN21AB8214.seq Check: 4898 from: 1 to: 5476
pFN21AB8214 5476 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2599 2599
Size: 5476
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3286 278
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2819 1937
Size: 882 4594
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 3603 3976 1563
Size: 2040 373 3063
Fragments arranged by size:
3063 2040 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3539 4923 5191 1632
Size: 1907 1384 268 1917
Fragments arranged by size:
1917 1907 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 4375 5352 1203
Size: 3172 977 1327
Fragments arranged by size:
3172 1327 977
AgeI A'CCGG_T
Cuts at: 2715 4307 2715
Size: 1592 3884
AhdI GACnn_n'nnGTC
Cuts at: 4205 4205
Size: 5476
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5476
AlwNI CAG_nnn'CTG
Cuts at: 2518 2659 3219 4791 2518
Size: 141 560 1572 3203
Fragments arranged by size:
3203 1572 560 141
ApaI G_GGCC'C
Cuts at: 2250 2250
Size: 5476
ApaLI G'TGCA_C
Cuts at: 3533 4689 3533
Size: 1156 4320
ApoI r'AATT_y
Cuts at: 1493 2785 3011 3163 5272 1493
Size: 1292 226 152 2109 1697
Fragments arranged by size:
2109 1697 1292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2249 2264 2801 1870
Size: 73 306 15 537 4545
Fragments arranged by size:
4545 537 306 73 15
BamHI G'GATC_C
Cuts at: 2487 2806 3173 2487
Size: 319 367 4790
Fragments arranged by size:
4790 367 319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2315 2351 2561 2797
Size: 325 217 627 279 249 36 210 236
Cuts at: 2797 4252 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 279 249 236 217
210 36
BanII G_rGCy'C
Cuts at: 1391 2102 2250 2256 2702 2795 1391
Size: 711 148 6 446 93 4072
Fragments arranged by size:
4072 711 446 148 93 6
BbeI G_GCGC'C
Cuts at: 2319 2355 2565 2319
Size: 36 210 5230
Fragments arranged by size:
5230 210 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4505
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3667 3701 1903
Size: 34 1730 34 3678
Fragments arranged by size:
3678 1730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2183 4087 136
Size: 107 122 71 631 605 511 1904 1525
Fragments arranged by size:
1904 1525 631 605 511 122 107 71
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2893 2893
Size: 5476
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2250 2439 3537 4693 1790
Size: 291 169 189 1098 1156 2573
Fragments arranged by size:
2573 1156 1098 291 189 169
BmgBI CAC'GTC
Cuts at: 1318 2434 1318
Size: 1116 4360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3207 4165 442
Size: 416 53 83 399 24 1790 958 1753
Fragments arranged by size:
1790 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2546 2681 4136 1239
Size: 1307 135 1455 2579
Fragments arranged by size:
2579 1455 1307 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3601 4466 4764 3601
Size: 865 298 4313
Fragments arranged by size:
4313 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4139 882
Size: 617 2640 2219
Fragments arranged by size:
2640 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2554 3267 493
Size: 711 1350 713 2702
Fragments arranged by size:
2702 1350 713 711
BsaBI GATnn'nnATC
Cuts at: 5470 5470
Size: 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 2352 2562 3283
Size: 53 83 186 936 783 36 210 721
Cuts at: 3283 3665 4300 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 783 721 635 382 210 186
83 53 36
BsaWI w'CCGG_w
Cuts at: 2631 2715 3909 4307 4581 4728 5139 2631
Size: 84 1194 398 274 147 411 2968
Fragments arranged by size:
2968 1194 411 398 274 147 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5251
Fragments arranged by size:
5251 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2678 1186
Size: 1492 3984
BseYI C'CCAG_C
Cuts at: 2168 2311 2355 4679 5175 2168
Size: 143 44 2324 496 2469
Fragments arranged by size:
2469 2324 496 143 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 1163
Size: 573 705 4198
Fragments arranged by size:
4198 705 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 2178 2214 2926 3687 4715
Size: 275 186 120 57 36 712 761 1028
Cuts at: 4715 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 712 275 186 120 57
36
BsiHKAI G_wGCw'C
Cuts at: 2256 3537 3622 4693 2256
Size: 1281 85 1071 3039
Fragments arranged by size:
3039 1281 1071 85
BsmI GAATG_Cn'
Cuts at: 2987 3080 2987
Size: 93 5383
BsmBI CGTCTCn'nnnn_
Cuts at: 2597 2597
Size: 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2102 2250 2256 2439 2702 2795
Size: 399 291 21 148 6 183 263 93
Cuts at: 2795 3537 3622 4693 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 291 263 183 148
93 85 21 6
BspEI T'CCGG_A
Cuts at: 5139 5139
Size: 5476
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2599 2831 844
Size: 1755 232 3489
Fragments arranged by size:
3489 1755 232
BsrBI CCG'CTC
Cuts at: 1394 1823 2137 2232 2569 3363 1394
Size: 429 314 95 337 794 3507
Fragments arranged by size:
3507 794 429 337 314 95
BsrDI GCAATG_nn'
Cuts at: 65 1248 2724 3971 4145 65
Size: 1183 1476 1247 174 1396
Fragments arranged by size:
1476 1396 1247 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2069 2582 2715 4120 4307 1700
Size: 110 259 513 133 1405 187 2869
Fragments arranged by size:
2869 1405 513 259 187 133 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5476
BssHII G'CGCG_C
Cuts at: 1925 2530 1925
Size: 605 4871
BssSI C'ACGA_G
Cuts at: 2255 3536 4548 2255
Size: 1281 1012 3183
Fragments arranged by size:
3183 1281 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2518 794
Size: 1724 3752
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5476
BstYI r'GATC_y
Cuts at: 1972 2487 2806 3173 3558 3575 5027 5113 5125
Size: 515 319 367 385 17 1452 86 12
Cuts at: 5125 5471 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 515 385 367 346 319 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2016 2145 2208 2217 513
Size: 552 951 129 63 9 3772
Fragments arranged by size:
3772 951 552 129 63 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4737
Fragments arranged by size:
4737 683 56
BtsI GCAGTG_nn'
Cuts at: 2992 3786 3806 2992
Size: 794 20 4662
Fragments arranged by size:
4662 794 20
ClaI AT'CG_AT
Cuts at: 1554 2757 1554
Size: 1203 4273
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2780 2780
Size: 5476
DraIII CAC_nnn'GTG
Cuts at: 2554 2554
Size: 5476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2759 4483 809
Size: 507 1443 1724 1802
Fragments arranged by size:
1802 1724 1443 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2052 2118
Size: 54 1425 50 275 18 189 33 66
Cuts at: 2118 2175 2211 2410 2580 2923 3812 5441 8
Size: 57 36 199 170 343 889 1629 43
Fragments arranged by size:
1629 1425 889 343 275 199 189 170
66 57 54 50 43 36 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2118 2175 2211 2923 1537
Size: 275 306 57 36 712 4090
Fragments arranged by size:
4090 712 306 275 57 36
EarI CTCTTCn'nnn_
Cuts at: 1353 3406 1353
Size: 2053 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4063 4437 4583 387
Size: 1064 138 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2254 2254
Size: 5476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2546 2681 3539 4136 4923 5191 1239
Size: 393 914 135 858 597 787 268 1524
Fragments arranged by size:
1524 914 858 787 597 393 268 135
EcoO109I rG'GnC_Cy
Cuts at: 2365 2389 2672 2365
Size: 24 283 5169
Fragments arranged by size:
5169 283 24
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 5476
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 2153 2319 2355 2565 4623 1678
Size: 475 166 36 210 2058 2531
Fragments arranged by size:
2531 2058 475 210 166 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2281 2313 2750 2782 4117
Size: 32 135 32 709 32 437 32 1335
Cuts at: 4117 4149 4191 4223 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1335 709 437 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2820 3066 669
Size: 988 281 882 246 3079
Fragments arranged by size:
3079 988 882 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KasI G'GCGC_C
Cuts at: 2315 2351 2561 2315
Size: 36 210 5230
Fragments arranged by size:
5230 210 36
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4590 4774 1305
Size: 3285 184 2007
Fragments arranged by size:
3285 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2054 10
Size: 54 1425 343 222 3432
Fragments arranged by size:
3432 1425 343 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3434 3793 3952 518
Size: 648 2268 359 159 2042
Fragments arranged by size:
2268 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2210 2219 2311 2326 2515 2578 2892
Size: 412 192 9 92 15 189 63 314
Cuts at: 2892 3569 4717 4962 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 412 314 245 192 189
92 63 15 9
NaeI GCC'GGC
Cuts at: 2584 2584
Size: 5476
NarI GG'CG_CC
Cuts at: 2316 2352 2562 2316
Size: 36 210 5230
Fragments arranged by size:
5230 210 36
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4924
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NgoMIV G'CCGG_C
Cuts at: 2582 2582
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2118 2211 2923 2118
Size: 93 712 4671
Fragments arranged by size:
4671 712 93
NspI r_CATG'y
Cuts at: 1132 1229 2834 4379 1132
Size: 97 1605 1545 2229
Fragments arranged by size:
2229 1605 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2385 1348
Size: 1037 4439
PciI A'CATG_T
Cuts at: 4375 4375
Size: 5476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2110 2554 1108
Size: 1002 444 4030
Fragments arranged by size:
4030 1002 444
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2243 2275 3545 3577 5058 5090 2243
Size: 32 1270 32 1481 32 2629
Fragments arranged by size:
2629 1481 1270 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2365 2389 2365
Size: 24 5452
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5476
PsiI TTA'TAA
Cuts at: 3046 3046
Size: 5476
PspOMI G'GGCC_C
Cuts at: 2246 2246
Size: 5476
PstI C_TGCA'G
Cuts at: 830 2331 2828 830
Size: 1501 497 3478
Fragments arranged by size:
3478 1501 497
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
PvuII CAG'CTG
Cuts at: 2311 2515 2578 2311
Size: 204 63 5209
Fragments arranged by size:
5209 204 63
SacI G_AGCT'C
Cuts at: 2256 2256
Size: 5476
SacII CC_GC'GG
Cuts at: 2019 2211 2220 2019
Size: 192 9 5275
Fragments arranged by size:
5275 192 9
SalI G'TCGA_C
Cuts at: 1936 2818 1936
Size: 882 4594
SanDI GG'GwC_CC
Cuts at: 2365 2365
Size: 5476
SbfI CC_TGCA'GG
Cuts at: 2828 2828
Size: 5476
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2327 2824 3959 4640 4831 826
Size: 219 1282 497 1135 681 191 1471
Fragments arranged by size:
1471 1282 1135 681 497 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2183 2183
Size: 5476
SfoI GGC'GCC
Cuts at: 2317 2353 2563 2317
Size: 36 210 5230
Fragments arranged by size:
5230 210 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 2069 1700
Size: 369 5107
SmaI CCC'GGG
Cuts at: 1872 2803 1872
Size: 931 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3580 4481 4743 820
Size: 197 926 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 3400 5
Size: 47 3348 2081
Fragments arranged by size:
3348 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5476
StyI C'CwwG_G
Cuts at: 513 1065 2046 2055 2915 513
Size: 552 981 9 860 3074
Fragments arranged by size:
3074 981 860 552 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3503 3520 3673 3858 4303 325
Size: 1255 1923 17 153 185 445 1498
Fragments arranged by size:
1923 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2643 3722 5434
Size: 274 80 33 51 1443 666 1079 1712
Cuts at: 5434 96
Size: 138
Fragments arranged by size:
1712 1443 1079 666 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5436 5468 5436
Size: 32 5444
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5476
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2044 2044
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5476
XmaI C'CCGG_G
Cuts at: 1870 2801 1870
Size: 931 4545
XmnI GAAnn'nnTTC
Cuts at: 2402 3605 2402
Size: 1203 4273
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3284 276
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SapI SexAI SrfI SwaI