Restriction map for
pFN21AB8211
(Circular) (Six-base) MAPSORT of: pFN21AB8211.seq Check: 5743 from: 1 to: 5794
pFN21AB8211 5794 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3604 278
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3137 1937
Size: 1200 4594
Acc65I G'GTAC_C
Cuts at: 3115 3115
Size: 5794
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3857 5241 5509 1632
Size: 2225 1384 268 1917
Fragments arranged by size:
2225 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5597
AflIII A'CryG_T
Cuts at: 1203 2802 4693 5670 1203
Size: 1599 1891 977 1327
Fragments arranged by size:
1891 1599 1327 977
AgeI A'CCGG_T
Cuts at: 4625 4625
Size: 5794
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 2100 1166
Size: 934 4860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2118 1184
Size: 934 4860
AlwNI CAG_nnn'CTG
Cuts at: 3537 5109 3537
Size: 1572 4222
ApaI G_GGCC'C
Cuts at: 2405 2571 2648 2748 2879 2405
Size: 166 77 100 131 5320
Fragments arranged by size:
5320 166 131 100 77
ApaLI G'TGCA_C
Cuts at: 3851 5007 3851
Size: 1156 4638
ApoI r'AATT_y
Cuts at: 1493 3103 3329 3481 5590 1493
Size: 1610 226 152 2109 1697
Fragments arranged by size:
2109 1697 1610 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4348 160
Size: 872 3316 1606
Fragments arranged by size:
3316 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 2932 2995 3055 3119 1870
Size: 73 989 63 60 64 4545
Fragments arranged by size:
4545 989 73 64 63 60
BamHI G'GATC_C
Cuts at: 2921 3124 3491 2921
Size: 203 367 5224
Fragments arranged by size:
5224 367 203
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2224 2474 2834 2969
Size: 325 217 627 315 122 250 360 135
Cuts at: 2969 2999 3115 4570 618
Size: 30 116 1455 1842
Fragments arranged by size:
1842 1455 627 360 325 315 250 217
135 122 116 30
BanII G_rGCy'C
Cuts at: 1391 2172 2405 2487 2500 2571 2648 2748 2879
Size: 781 233 82 13 71 77 100 131
Cuts at: 2879 2933 3113 1391
Size: 54 180 4072
Fragments arranged by size:
4072 781 233 180 131 100 82 77
71 54 13
BbeI G_GCGC'C
Cuts at: 2228 2478 2838 2973 2228
Size: 250 360 135 5049
Fragments arranged by size:
5049 360 250 135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2194 928
Size: 971 295 4528
Fragments arranged by size:
4528 971 295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3985 4019 1903
Size: 34 2048 34 3678
Fragments arranged by size:
3678 2048 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2451 2843 2874
Size: 107 122 71 631 605 779 392 31
Cuts at: 2874 4405 136
Size: 1531 1525
Fragments arranged by size:
1531 1525 779 631 605 392 122 107
71 31
BglII A'GATC_T
Cuts at: 5789 5789
Size: 5794
BlpI GC'TnA_GC
Cuts at: 3211 3211
Size: 5794
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2107 2405 2571 2648 2748 2879 3002
Size: 250 67 298 166 77 100 131 123
Cuts at: 3002 3855 5011 1790
Size: 853 1156 2573
Fragments arranged by size:
2573 1156 853 298 250 166 131 123
100 77 67
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2670 3525 4483
Size: 416 53 83 399 24 1253 855 958
Cuts at: 4483 442
Size: 1753
Fragments arranged by size:
1753 1253 958 855 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2451 2451
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2776 2796 4454 1239
Size: 1537 20 1658 2579
Fragments arranged by size:
2579 1658 1537 20
Bpu10I CC'TnA_GC
Cuts at: 2984 2984
Size: 5794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3919 4784 5082 3919
Size: 865 298 4631
Fragments arranged by size:
4631 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4457 882
Size: 617 2958 2219
Fragments arranged by size:
2958 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2914 3585 493
Size: 711 1710 671 2702
Fragments arranged by size:
2702 1710 711 671
BsaBI GATnn'nnATC
Cuts at: 5788 5788
Size: 5794
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2225 2475 2540 2835
Size: 53 83 186 936 692 250 65 295
Cuts at: 2835 2970 3601 3983 4618 275
Size: 135 631 382 635 1451
Fragments arranged by size:
1451 936 692 635 631 382 295 250
186 135 83 65 53
BsaWI w'CCGG_w
Cuts at: 2723 4227 4625 4899 5046 5457 2723
Size: 1504 398 274 147 411 3060
Fragments arranged by size:
3060 1504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5569
Fragments arranged by size:
5569 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 2535 2689 2692 2751 1186
Size: 1270 79 154 3 59 4229
Fragments arranged by size:
4229 1270 154 79 59 3
BseYI C'CCAG_C
Cuts at: 2161 2300 2457 4997 5493 2161
Size: 139 157 2540 496 2462
Fragments arranged by size:
2540 2462 496 157 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2078 2168 3029 1163
Size: 573 342 90 861 3928
Fragments arranged by size:
3928 861 573 342 90
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 3030 3244 4005 5033 1540
Size: 275 186 33 996 214 761 1028 2301
Fragments arranged by size:
2301 1028 996 761 275 214 186 33
BsiHKAI G_wGCw'C
Cuts at: 2253 3855 3940 5011 2253
Size: 1602 85 1071 3036
Fragments arranged by size:
3036 1602 1071 85
BsmI GAATG_Cn'
Cuts at: 3305 3398 3305
Size: 93 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2107 2172 2253 2405 2487 2500
Size: 399 250 67 65 81 152 82 13
Cuts at: 2500 2571 2648 2748 2879 2933 3002 3113 3855
Size: 71 77 100 131 54 69 111 742
Cuts at: 3855 3940 5011 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 250 152 131 111
100 85 82 81 77 71 69 67
65 54 13
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 2814 3683 5413 2814
Size: 869 1730 3195
Fragments arranged by size:
3195 1730 869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 3149 844
Size: 1253 1052 3489
Fragments arranged by size:
3489 1253 1052
BsrBI CCG'CTC
Cuts at: 1394 1823 2857 3681 1394
Size: 429 1034 824 3507
Fragments arranged by size:
3507 1034 824 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4289 4463 65
Size: 1183 3041 174 1396
Fragments arranged by size:
3041 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2615 2837 2978 4438 4625 1700
Size: 110 805 222 141 1460 187 2869
Fragments arranged by size:
2869 1460 805 222 187 141 110
BsrGI T'GTAC_A
Cuts at: 96 2805 96
Size: 2709 3085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 2493 2547 3854 4866 2493
Size: 54 1307 1012 3421
Fragments arranged by size:
3421 1307 1012 54
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5794
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5794
BstXI CCAn_nnnn'nTGG
Cuts at: 2579 2641 3091 2579
Size: 62 450 5282
Fragments arranged by size:
5282 450 62
BstYI r'GATC_y
Cuts at: 1972 2136 2921 3124 3491 3876 3893 5345 5431
Size: 164 785 203 367 385 17 1452 86
Cuts at: 5431 5443 5789 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 785 385 367 346 203 164
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2352 2478 2663 2880 2973 3005 513
Size: 552 1287 126 185 217 93 32 3302
Fragments arranged by size:
3302 1287 552 217 185 126 93 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2912 487
Size: 683 56 1686 3369
Fragments arranged by size:
3369 1686 683 56
BtsI GCAGTG_nn'
Cuts at: 3310 4104 4124 3310
Size: 794 20 4980
Fragments arranged by size:
4980 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5759
DraI TTT'AAA
Cuts at: 3098 3098
Size: 5794
DraIII CAC_nnn'GTG
Cuts at: 2914 2914
Size: 5794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2128 4801 809
Size: 507 812 2673 1802
Fragments arranged by size:
2673 1802 812 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2340 2556
Size: 54 1425 50 275 18 201 309 216
Cuts at: 2556 2883 2976 3027 3241 4130 5759 8
Size: 327 93 51 214 889 1629 43
Fragments arranged by size:
1629 1425 889 327 309 275 216 214
201 93 54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 3027 3241 1537
Size: 275 219 996 214 4090
Fragments arranged by size:
4090 996 275 219 214
EarI CTCTTCn'nnn_
Cuts at: 1353 2230 2951 3724 1353
Size: 877 721 773 3423
Fragments arranged by size:
3423 877 773 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4381 4755 4901 387
Size: 1064 138 2792 374 146 1280
Fragments arranged by size:
2792 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2776 2796 3857 4454 5241 5509 1239
Size: 393 1144 20 1061 597 787 268 1524
Fragments arranged by size:
1524 1144 1061 787 597 393 268 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2439 2439
Size: 5794
EcoO109I rG'GnC_Cy
Cuts at: 2209 2401 2402 2567 2568 2745 2875 2876 3036
Size: 192 1 165 1 177 130 1 160
Cuts at: 3036 2209
Size: 4967
Fragments arranged by size:
4967 192 177 165 160 130 1 1
1
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
FseI GG_CCGG'CC
Cuts at: 2982 2982
Size: 5794
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 2072 2228 2478 2838 2973 4941 1678
Size: 394 156 250 360 135 1968 2531
Fragments arranged by size:
2531 1968 394 360 250 156 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2413 2445 2530 2562 2890
Size: 32 135 32 841 32 85 32 328
Cuts at: 2890 2922 4435 4467 4509 4541 1373
Size: 32 1513 32 42 32 2626
Fragments arranged by size:
2626 1513 841 328 135 85 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3138 3384 669
Size: 988 281 1200 246 3079
Fragments arranged by size:
3079 1200 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KasI G'GCGC_C
Cuts at: 2224 2474 2834 2969 2224
Size: 250 360 135 5049
Fragments arranged by size:
5049 360 250 135
KpnI G_GTAC'C
Cuts at: 3119 3119
Size: 5794
MfeI C'AATT_G
Cuts at: 3393 3393
Size: 5794
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2067 4908 5092 1305
Size: 762 2841 184 2007
Fragments arranged by size:
2841 2007 762 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2558 10
Size: 54 1425 343 726 3246
Fragments arranged by size:
3246 1425 726 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2100 2639 2813 3089 3752 4111 4270
Size: 648 934 539 174 276 663 359 159
Cuts at: 4270 518
Size: 2042
Fragments arranged by size:
2042 934 663 648 539 359 276 174
159
MspA1I CmG'CkG
Cuts at: 1606 3007 3210 3887 5035 5280 1606
Size: 1401 203 677 1148 245 2120
Fragments arranged by size:
2120 1401 1148 677 245 203
NaeI GCC'GGC
Cuts at: 2617 2839 2980 2617
Size: 222 141 5431
Fragments arranged by size:
5431 222 141
NarI GG'CG_CC
Cuts at: 2225 2475 2835 2970 2225
Size: 250 360 135 5049
Fragments arranged by size:
5049 360 250 135
NcoI C'CATG_G
Cuts at: 513 1065 2352 2478 2880 513
Size: 552 1287 126 402 3427
Fragments arranged by size:
3427 1287 552 402 126
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NgoMIV G'CCGG_C
Cuts at: 2615 2837 2978 2615
Size: 222 141 5431
Fragments arranged by size:
5431 222 141
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3027 3241 3027
Size: 214 5580
NspI r_CATG'y
Cuts at: 1132 1229 2593 2806 3152 4697 1132
Size: 97 1364 213 346 1545 2229
Fragments arranged by size:
2229 1545 1364 346 213 97
PasI CC'CwG_GG
Cuts at: 1348 2174 2396 2500 2926 1348
Size: 826 222 104 426 4216
Fragments arranged by size:
4216 826 426 222 104
PciI A'CATG_T
Cuts at: 2802 4693 2802
Size: 1891 3903
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5794
PfoI T'CCnGG_A
Cuts at: 2274 2274
Size: 5794
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PmlI CAC'GTG
Cuts at: 2914 2914
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 5376 5408 3863
Size: 32 1481 32 4249
Fragments arranged by size:
4249 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2209 3036 2209
Size: 827 4967
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5794
PsiI TTA'TAA
Cuts at: 3364 3364
Size: 5794
PspOMI G'GGCC_C
Cuts at: 2401 2567 2644 2744 2875 2401
Size: 166 77 100 131 5320
Fragments arranged by size:
5320 166 131 100 77
PstI C_TGCA'G
Cuts at: 830 2293 3146 830
Size: 1463 853 3478
Fragments arranged by size:
3478 1463 853
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
SacII CC_GC'GG
Cuts at: 3008 3008
Size: 5794
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SbfI CC_TGCA'GG
Cuts at: 3146 3146
Size: 5794
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SfcI C'TryA_G
Cuts at: 826 1045 2281 2289 3142 4277 4958 5149 826
Size: 219 1236 8 853 1135 681 191 1471
Fragments arranged by size:
1471 1236 1135 853 681 219 191 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 2874 2874
Size: 5794
SfoI GGC'GCC
Cuts at: 2226 2476 2836 2971 2226
Size: 250 360 135 5049
Fragments arranged by size:
5049 360 250 135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5794
SmaI CCC'GGG
Cuts at: 1872 2997 3121 1872
Size: 1125 124 4545
Fragments arranged by size:
4545 1125 124
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3898 4799 5061 820
Size: 197 926 1955 901 262 1553
Fragments arranged by size:
1955 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 2593 3152 1132
Size: 1461 559 3774
Fragments arranged by size:
3774 1461 559
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 2243 2270 2445 2625 1517
Size: 726 27 175 180 4686
Fragments arranged by size:
4686 726 180 175 27
StyI C'CwwG_G
Cuts at: 513 1065 2013 2265 2352 2478 2880 3233 513
Size: 552 948 252 87 126 402 353 3074
Fragments arranged by size:
3074 948 552 402 353 252 126 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2617 2773 3821 3838 3991 4176 4621
Size: 1255 1037 156 1048 17 153 185 445
Cuts at: 4621 325
Size: 1498
Fragments arranged by size:
1498 1255 1048 1037 445 185 156 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 2805 4040
Size: 274 80 33 51 1443 237 591 1235
Cuts at: 4040 5752 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1235 591 274 237 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2376 2408 5754 5786 2376
Size: 32 3346 32 2384
Fragments arranged by size:
3346 2384 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5794
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2308 2896 2308
Size: 588 5206
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5794
XmaI C'CCGG_G
Cuts at: 1870 2995 3119 1870
Size: 1125 124 4545
Fragments arranged by size:
4545 1125 124
XmnI GAAnn'nnTTC
Cuts at: 3923 3923
Size: 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3602 276
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FspAI
NruI NsiI PacI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SrfI SwaI