Restriction map for
pFN21AB8209
(Circular) (Six-base) MAPSORT of: pFN21AB8209.seq Check: 8847 from: 1 to: 5521
pFN21AB8209 5521 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2377 2864 1937
Size: 440 487 4594
Fragments arranged by size:
4594 487 440
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3584 4968 5236 1632
Size: 1952 1384 268 1917
Fragments arranged by size:
1952 1917 1384 268
AfeI AGC'GCT
Cuts at: 2513 2513
Size: 5521
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 2224 4420 5397 1203
Size: 1021 2196 977 1327
Fragments arranged by size:
2196 1327 1021 977
AgeI A'CCGG_T
Cuts at: 2247 4352 2247
Size: 2105 3416
AhdI GACnn_n'nnGTC
Cuts at: 4250 4250
Size: 5521
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5521
AlwNI CAG_nnn'CTG
Cuts at: 2282 2662 3264 4836 2282
Size: 380 602 1572 2967
Fragments arranged by size:
2967 1572 602 380
ApaI G_GGCC'C
Cuts at: 2671 2671
Size: 5521
ApaLI G'TGCA_C
Cuts at: 3578 4734 3578
Size: 1156 4365
ApoI r'AATT_y
Cuts at: 1493 2830 3056 3208 5317 1493
Size: 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2846 1870
Size: 73 903 4545
Fragments arranged by size:
4545 903 73
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2396 2842 4297 618
Size: 325 217 627 609 446 1455 1842
Fragments arranged by size:
1842 1455 627 609 446 325 217
BanII G_rGCy'C
Cuts at: 1391 2671 2707 2751 2773 2840 1391
Size: 1280 36 44 22 67 4072
Fragments arranged by size:
4072 1280 67 44 36 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2221 928
Size: 971 322 4228
Fragments arranged by size:
4228 971 322
BbvCI CC'TCA_GC
Cuts at: 2241 2471 2241
Size: 230 5291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2001 2035 3712 3746 1903
Size: 34 64 34 1677 34 3678
Fragments arranged by size:
3678 1677 64 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2622 4132
Size: 107 122 71 631 605 337 613 1510
Cuts at: 4132 136
Size: 1525
Fragments arranged by size:
1525 1510 631 613 605 337 122 107
71
BglII A'GATC_T
Cuts at: 5516 5516
Size: 5521
BlpI GC'TnA_GC
Cuts at: 2938 2938
Size: 5521
Bme1580I G_kGCm'C
Cuts at: 1790 2401 2671 3582 4738 1790
Size: 611 270 911 1156 2573
Fragments arranged by size:
2573 1156 911 611 270
BmgBI CAC'GTC
Cuts at: 1318 2188 2696 1318
Size: 870 508 4143
Fragments arranged by size:
4143 870 508
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3252 4210 442
Size: 416 53 83 399 24 1835 958 1753
Fragments arranged by size:
1835 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4181 1239
Size: 2942 2579
Bpu10I CC'TnA_GC
Cuts at: 2241 2471 2241
Size: 230 5291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2517 2536 3646 4511 4809 2423
Size: 94 19 1110 865 298 3135
Fragments arranged by size:
3135 1110 865 298 94 19
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 4184 882
Size: 617 1041 1644 2219
Fragments arranged by size:
2219 1644 1041 617
BsaAI yAC'GTr
Cuts at: 493 1204 3312 493
Size: 711 2108 2702
Fragments arranged by size:
2702 2108 711
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3328 3710 4345 275
Size: 53 83 186 936 1795 382 635 1451
Fragments arranged by size:
1795 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2247 3954 4352 4626 4773 5184 2247
Size: 1707 398 274 147 411 2584
Fragments arranged by size:
2584 1707 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5296
Fragments arranged by size:
5296 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2099 1186
Size: 910 3 4608
Fragments arranged by size:
4608 910 3
BseYI C'CCAG_C
Cuts at: 2640 4724 5220 2640
Size: 2084 496 2941
Fragments arranged by size:
2941 2084 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 1163
Size: 573 432 4516
Fragments arranged by size:
4516 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2598 2971 3732 4760 1540
Size: 275 186 597 373 761 1028 2301
Fragments arranged by size:
2301 1028 761 597 373 275 186
BsiHKAI G_wGCw'C
Cuts at: 2481 3582 3667 4738 2481
Size: 1101 85 1071 3264
Fragments arranged by size:
3264 1101 1071 85
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
BsmBI CGTCTCn'nnnn_
Cuts at: 2251 2251
Size: 5521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2401 2481 2671 2707 2751 2773 2840
Size: 399 611 80 190 36 44 22 67
Cuts at: 2840 3582 3667 4738 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 611 399 190 85 80
67 44 36 22
BspEI T'CCGG_A
Cuts at: 5184 5184
Size: 5521
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2876 844
Size: 2032 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2522 2736 3408 1394
Size: 429 699 214 672 3507
Fragments arranged by size:
3507 699 672 429 214
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2247 4165 4352 1700
Size: 110 437 1918 187 2869
Fragments arranged by size:
2869 1918 437 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5521
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5521
BssSI C'ACGA_G
Cuts at: 3581 4593 3581
Size: 1012 4509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2282 794
Size: 1488 4033
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5521
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 2365
Size: 5521
BstYI r'GATC_y
Cuts at: 1972 2760 2851 3218 3603 3620 5072 5158 5170
Size: 788 91 367 385 17 1452 86 12
Cuts at: 5170 5516 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 788 385 367 346 91 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4030
Fragments arranged by size:
4030 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4782
Fragments arranged by size:
4782 683 56
BtsI GCAGTG_nn'
Cuts at: 3037 3831 3851 3037
Size: 794 20 4707
Fragments arranged by size:
4707 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2825 2825
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4528 809
Size: 507 3212 1802
Fragments arranged by size:
3212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 2595 2684
Size: 54 1425 50 275 18 540 225 89
Cuts at: 2684 2754 2968 3857 5486 8
Size: 70 214 889 1629 43
Fragments arranged by size:
1629 1425 889 540 275 225 214 89
70 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2595 2968 1537
Size: 275 783 373 4090
Fragments arranged by size:
4090 783 373 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3451 1353
Size: 2098 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4108 4482 4628 387
Size: 1064 138 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3584 4181 4968 5236 1239
Size: 393 1952 597 787 268 1524
Fragments arranged by size:
1952 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2605 2667 2798 2605
Size: 62 131 5328
Fragments arranged by size:
5328 131 62
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
FspI TGC'GCA
Cuts at: 2504 4027 2504
Size: 1523 3998
HaeII r_GCGC'y
Cuts at: 1678 2208 2497 2515 4668 1678
Size: 530 289 18 2153 2531
Fragments arranged by size:
2531 2153 530 289 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4162 4194 4236 4268 1373
Size: 32 135 32 2590 32 42 32 2626
Fragments arranged by size:
2626 2590 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3111 669
Size: 988 281 927 246 3079
Fragments arranged by size:
3079 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 3120 3120
Size: 5521
MluI A'CGCG_T
Cuts at: 2224 5397 2224
Size: 3173 2348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2200 2763 4635 4819 1305
Size: 895 563 1872 184 2007
Fragments arranged by size:
2007 1872 895 563 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2756 10
Size: 54 1425 343 924 2775
Fragments arranged by size:
2775 1425 924 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 3479 3838 3997 518
Size: 648 1224 1089 359 159 2042
Fragments arranged by size:
2042 1224 1089 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2282 2937 3614 4762 5007 1606
Size: 444 232 655 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 655 444 245 232
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4030
Fragments arranged by size:
4030 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2968 2968
Size: 5521
NspI r_CATG'y
Cuts at: 1132 1229 2879 4424 1132
Size: 97 1650 1545 2229
Fragments arranged by size:
2229 1650 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5521
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5521
PfoI T'CCnGG_A
Cuts at: 2627 2627
Size: 5521
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2234 2266 3590 3622 5103 5135 2234
Size: 32 1324 32 1481 32 2620
Fragments arranged by size:
2620 1481 1324 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2605 2798 2605
Size: 193 5328
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PspOMI G'GGCC_C
Cuts at: 2667 2667
Size: 5521
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2426 2458 2426
Size: 32 5489
PstI C_TGCA'G
Cuts at: 830 2873 830
Size: 2043 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
PvuII CAG'CTG
Cuts at: 2282 2282
Size: 5521
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SanDI GG'GwC_CC
Cuts at: 2605 2605
Size: 5521
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 2444 3769 5481 2444
Size: 1325 1712 2484
Fragments arranged by size:
2484 1712 1325
SexAI A'CCwGG_T
Cuts at: 2459 2459
Size: 5521
SfcI C'TryA_G
Cuts at: 826 1045 2869 4004 4685 4876 826
Size: 219 1824 1135 681 191 1471
Fragments arranged by size:
1824 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5521
SmaI CCC'GGG
Cuts at: 1872 2848 1872
Size: 976 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2438 2515 2532 3625 4526 4788
Size: 197 926 495 77 17 1093 901 262
Cuts at: 4788 820
Size: 1553
Fragments arranged by size:
1553 1093 926 901 495 262 197 77
17
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2879 1132
Size: 1747 3774
SspI AAT'ATT
Cuts at: 5 52 3445 5
Size: 47 3393 2081
Fragments arranged by size:
3393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2310 2960 513
Size: 552 939 306 650 3074
Fragments arranged by size:
3074 939 650 552 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2320 2384 2564 3548 3565 3718 3903
Size: 1255 740 64 180 984 17 153 185
Cuts at: 3903 4348 325
Size: 445 1498
Fragments arranged by size:
1498 1255 984 740 445 185 180 153
64 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2442 3767 5479
Size: 274 80 33 51 1443 465 1325 1712
Cuts at: 5479 96
Size: 138
Fragments arranged by size:
1712 1443 1325 465 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5481 5513 5481
Size: 32 5489
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2625 2625
Size: 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5521
XmaI C'CCGG_G
Cuts at: 1870 2846 1870
Size: 976 4545
XmnI GAAnn'nnTTC
Cuts at: 3650 3650
Size: 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI RsrII SacI SacII SapI SfiI
SfoI SrfI SwaI