Restriction map for
pFN21AB8204
(Circular) (Six-base) MAPSORT of: pFN21AB8204.seq Check: 775 from: 1 to: 6115
pFN21AB8204 6115 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3925 278
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3458 1937
Size: 1521 4594
Acc65I G'GTAC_C
Cuts at: 3436 3436
Size: 6115
AclI AA'CG_TT
Cuts at: 1563 4242 4615 1563
Size: 2679 373 3063
Fragments arranged by size:
3063 2679 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2003 2426 2945 2997 3309 4178 5562 5830
Size: 371 423 519 52 312 869 1384 268
Cuts at: 5830 1632
Size: 1917
Fragments arranged by size:
1917 1384 869 519 423 371 312 268
52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5918
AflIII A'CryG_T
Cuts at: 1203 5014 5991 1203
Size: 3811 977 1327
Fragments arranged by size:
3811 1327 977
AgeI A'CCGG_T
Cuts at: 4946 4946
Size: 6115
AhdI GACnn_n'nnGTC
Cuts at: 2013 4844 2013
Size: 2831 3284
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6115
AlwNI CAG_nnn'CTG
Cuts at: 2824 3385 3397 3858 5430 2824
Size: 561 12 461 1572 3509
Fragments arranged by size:
3509 1572 561 461 12
ApaI G_GGCC'C
Cuts at: 2099 2099
Size: 6115
ApaLI G'TGCA_C
Cuts at: 4172 5328 4172
Size: 1156 4959
ApoI r'AATT_y
Cuts at: 1493 3424 3650 3802 5911 1493
Size: 1931 226 152 2109 1697
Fragments arranged by size:
2109 1931 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4669 160
Size: 872 3637 1606
Fragments arranged by size:
3637 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2101 2192 2315 3440 1870
Size: 73 105 53 91 123 1125 4545
Fragments arranged by size:
4545 1125 123 105 91 73 53
BamHI G'GATC_C
Cuts at: 2761 3445 3812 2761
Size: 684 367 5064
Fragments arranged by size:
5064 684 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2086 2227 3436 4891 618
Size: 325 217 627 299 141 1209 1455 1842
Fragments arranged by size:
1842 1455 1209 627 325 299 217 141
BanII G_rGCy'C
Cuts at: 1391 2068 2099 2108 2166 3434 1391
Size: 677 31 9 58 1268 4072
Fragments arranged by size:
4072 1268 677 58 31 9
BbeI G_GCGC'C
Cuts at: 2090 2090
Size: 6115
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2009 2846 3032 928
Size: 971 110 837 186 4011
Fragments arranged by size:
4011 971 837 186 110
BbvCI CC'TCA_GC
Cuts at: 2481 2481
Size: 6115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4306 4340 1903
Size: 34 2369 34 3678
Fragments arranged by size:
3678 2369 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4007 5217 4007
Size: 1210 4905
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2103 4726 136
Size: 107 122 71 631 605 431 2623 1525
Fragments arranged by size:
2623 1525 631 605 431 122 107 71
BglII A'GATC_T
Cuts at: 2608 6110 2608
Size: 3502 2613
BlpI GC'TnA_GC
Cuts at: 2806 3194 3532 2806
Size: 388 338 5389
Fragments arranged by size:
5389 388 338
Bme1580I G_kGCm'C
Cuts at: 1790 2099 2232 4176 5332 1790
Size: 309 133 1944 1156 2573
Fragments arranged by size:
2573 1944 1156 309 133
BmgBI CAC'GTC
Cuts at: 1318 3049 1318
Size: 1731 4384
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2743 3846 4804
Size: 416 53 83 399 24 1326 1103 958
Cuts at: 4804 442
Size: 1753
Fragments arranged by size:
1753 1326 1103 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1995 2082 3187 3407 4775 1239
Size: 756 87 1105 220 1368 2579
Fragments arranged by size:
2579 1368 1105 756 220 87
Bpu10I CC'TnA_GC
Cuts at: 2481 2481
Size: 6115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3155 3242 4240 5105 5403 3155
Size: 87 998 865 298 3867
Fragments arranged by size:
3867 998 865 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3201 4778 882
Size: 617 1702 1577 2219
Fragments arranged by size:
2219 1702 1577 617
BsaAI yAC'GTr
Cuts at: 493 1204 3906 493
Size: 711 2702 2702
Fragments arranged by size:
2702 2702 711
BsaBI GATnn'nnATC
Cuts at: 6109 6109
Size: 6115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2087 3922 4304 4939
Size: 53 83 186 936 554 1835 382 635
Cuts at: 4939 275
Size: 1451
Fragments arranged by size:
1835 1451 936 635 554 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4548 4946 5220 5367 5778 4548
Size: 398 274 147 411 4885
Fragments arranged by size:
4885 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2239 2269 1178
Size: 30 165 30 836 30 5024
Fragments arranged by size:
5024 836 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2278 2714 3074 3308 1186
Size: 1092 436 360 234 3993
Fragments arranged by size:
3993 1092 436 360 234
BseYI C'CCAG_C
Cuts at: 2546 2782 2943 5318 5814 2546
Size: 236 161 2375 496 2847
Fragments arranged by size:
2847 2375 496 236 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2698 2774 1163
Size: 573 962 76 4504
Fragments arranged by size:
4504 962 573 76
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3565 4326 5354 1540
Size: 275 186 1564 761 1028 2301
Fragments arranged by size:
2301 1564 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3127 4176 4261 5332 3127
Size: 1049 85 1071 3910
Fragments arranged by size:
3910 1071 1049 85
BsmI GAATG_Cn'
Cuts at: 3626 3719 3626
Size: 93 6022
BsmBI CGTCTCn'nnnn_
Cuts at: 2660 3140 2660
Size: 480 5635
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2099 2108 2166 2232 3127 3434
Size: 399 278 31 9 58 66 895 307
Cuts at: 3434 4176 4261 5332 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 895 742 399 307 278 85
66 58 31 9
BspEI T'CCGG_A
Cuts at: 5778 5778
Size: 6115
BspHI T'CATG_A
Cuts at: 4004 5734 4004
Size: 1730 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3240 3470 844
Size: 2396 230 3489
Fragments arranged by size:
3489 2396 230
BsrBI CCG'CTC
Cuts at: 1394 1823 2805 3240 4002 1394
Size: 429 982 435 762 3507
Fragments arranged by size:
3507 982 762 435 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2499 4610 4784 65
Size: 1183 1251 2111 174 1396
Fragments arranged by size:
2111 1396 1251 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2224 3278 4759 4946 1700
Size: 110 414 1054 1481 187 2869
Fragments arranged by size:
2869 1481 1054 414 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6115
BssSI C'ACGA_G
Cuts at: 4175 5187 4175
Size: 1012 5103
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6115
BstBI TT'CG_AA
Cuts at: 4919 4919
Size: 6115
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6115
BstYI r'GATC_y
Cuts at: 1972 2608 2761 3138 3174 3363 3445 3812 4197
Size: 636 153 377 36 189 82 367 385
Cuts at: 4197 4214 5666 5752 5764 6110 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 636 385 377 367 346 189
153 86 82 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2034 2091 2151 3099 513
Size: 552 969 57 60 948 3529
Fragments arranged by size:
3529 969 948 552 60 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5376
Fragments arranged by size:
5376 683 56
BtsI GCAGTG_nn'
Cuts at: 2260 3631 4425 4445 2260
Size: 1371 794 20 3930
Fragments arranged by size:
3930 1371 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6080
DraI TTT'AAA
Cuts at: 3419 3419
Size: 6115
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2981 3154 5122 809
Size: 507 1665 173 1968 1802
Fragments arranged by size:
1968 1802 1665 507 173
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3102 3562 4451
Size: 54 1425 50 275 18 1272 460 889
Cuts at: 4451 6080 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1272 889 460 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3562 1537
Size: 275 1750 4090
Fragments arranged by size:
4090 1750 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2402 2575 3285 4045 1353
Size: 1049 173 710 760 3423
Fragments arranged by size:
3423 1049 760 710 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4702 5076 5222 387
Size: 1064 138 3113 374 146 1280
Fragments arranged by size:
3113 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2003 2082 2426 2945 2997 3187
Size: 393 363 8 79 344 519 52 190
Cuts at: 3187 3309 3407 4178 4775 5562 5830 1239
Size: 122 98 771 597 787 268 1524
Fragments arranged by size:
1524 787 771 597 519 393 363 344
268 190 122 98 79 52 8
EcoO109I rG'GnC_Cy
Cuts at: 2096 2698 2821 3253 2096
Size: 602 123 432 4958
Fragments arranged by size:
4958 602 432 123
EcoRI G'AATT_C
Cuts at: 3424 3802 3424
Size: 378 5737
FspI TGC'GCA
Cuts at: 3407 4621 3407
Size: 1214 4901
HaeII r_GCGC'y
Cuts at: 1678 2047 2090 2186 2950 3301 5262 1678
Size: 369 43 96 764 351 1961 2531
Fragments arranged by size:
2531 1961 764 369 351 96 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2430 2462 3109
Size: 32 135 32 427 32 399 32 647
Cuts at: 3109 3141 4756 4788 4830 4862 1373
Size: 32 1615 32 42 32 2626
Fragments arranged by size:
2626 1615 647 427 399 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3459 3705 669
Size: 988 281 1521 246 3079
Fragments arranged by size:
3079 1521 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3196 748
Size: 2448 3667
HpaI GTT'AAC
Cuts at: 3705 3705
Size: 6115
KasI G'GCGC_C
Cuts at: 2086 2086
Size: 6115
KpnI G_GTAC'C
Cuts at: 3440 3440
Size: 6115
MfeI C'AATT_G
Cuts at: 2979 3714 2979
Size: 735 5380
MluI A'CGCG_T
Cuts at: 5991 5991
Size: 6115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2475 2514 5229 5413 1305
Size: 1170 39 2715 184 2007
Fragments arranged by size:
2715 2007 1170 184 39
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4293
Fragments arranged by size:
4293 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3002 3047 4073 4432 4591 518
Size: 648 1836 45 1026 359 159 2042
Fragments arranged by size:
2042 1836 1026 648 359 159 45
MspA1I CmG'CkG
Cuts at: 1606 2153 3531 4208 5356 5601 1606
Size: 547 1378 677 1148 245 2120
Fragments arranged by size:
2120 1378 1148 677 547 245
NaeI GCC'GGC
Cuts at: 3280 3280
Size: 6115
NarI GG'CG_CC
Cuts at: 2087 2087
Size: 6115
NcoI C'CATG_G
Cuts at: 513 1065 2091 513
Size: 552 1026 4537
Fragments arranged by size:
4537 1026 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6115
NgoMIV G'CCGG_C
Cuts at: 3278 3278
Size: 6115
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6115
NotI GC'GGCC_GC
Cuts at: 3562 3562
Size: 6115
NspI r_CATG'y
Cuts at: 1132 1229 3473 5018 1132
Size: 97 2244 1545 2229
Fragments arranged by size:
2244 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6115
PciI A'CATG_T
Cuts at: 5014 5014
Size: 6115
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6115
PfoI T'CCnGG_A
Cuts at: 2394 2535 2394
Size: 141 5974
PmeI GTTT'AAAC
Cuts at: 3419 3419
Size: 6115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4184 4216 5697 5729 4184
Size: 32 1481 32 4570
Fragments arranged by size:
4570 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2698 3253 2698
Size: 555 5560
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6115
PsiI TTA'TAA
Cuts at: 3685 3685
Size: 6115
PspOMI G'GGCC_C
Cuts at: 2095 2095
Size: 6115
PstI C_TGCA'G
Cuts at: 830 2856 3467 830
Size: 2026 611 3478
Fragments arranged by size:
3478 2026 611
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6115
SacII CC_GC'GG
Cuts at: 2154 2154
Size: 6115
SalI G'TCGA_C
Cuts at: 1936 3457 1936
Size: 1521 4594
SbfI CC_TGCA'GG
Cuts at: 3467 3467
Size: 6115
ScaI AGT'ACT
Cuts at: 4363 6075 4363
Size: 1712 4403
SfcI C'TryA_G
Cuts at: 826 1045 2852 3463 4598 5279 5470 826
Size: 219 1807 611 1135 681 191 1471
Fragments arranged by size:
1807 1471 1135 681 611 219 191
SfoI GGC'GCC
Cuts at: 2088 2088
Size: 6115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6115
SmaI CCC'GGG
Cuts at: 1872 2050 2103 2194 3442 1872
Size: 178 53 91 1248 4545
Fragments arranged by size:
4545 1248 178 91 53
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3170 3257 4219 5120 5382 820
Size: 197 926 1227 87 962 901 262 1553
Fragments arranged by size:
1553 1227 962 926 901 262 197 87
SnaBI TAC'GTA
Cuts at: 493 3906 493
Size: 3413 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6115
SphI G_CATG'C
Cuts at: 1132 3473 1132
Size: 2341 3774
SrfI GCCC'GGGC
Cuts at: 2194 2194
Size: 6115
SspI AAT'ATT
Cuts at: 5 52 4039 5
Size: 47 3987 2081
Fragments arranged by size:
3987 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6115
StyI C'CwwG_G
Cuts at: 513 1065 2091 3554 513
Size: 552 1026 1463 3074
Fragments arranged by size:
3074 1463 1026 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2040 2044 2668 4142 4159 4312 4497
Size: 1255 460 4 624 1474 17 153 185
Cuts at: 4497 4942 325
Size: 445 1498
Fragments arranged by size:
1498 1474 1255 624 460 445 185 153
17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4361 6073 96
Size: 274 80 33 51 1443 2384 1712 138
Fragments arranged by size:
2384 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2239 2271 6075 6107 2239
Size: 32 3804 32 2247
Fragments arranged by size:
3804 2247 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6115
XbaI T'CTAG_A
Cuts at: 3451 3451
Size: 6115
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6115
XmaI C'CCGG_G
Cuts at: 1870 2048 2101 2192 3440 1870
Size: 178 53 91 1248 4545
Fragments arranged by size:
4545 1248 178 91 53
XmnI GAAnn'nnTTC
Cuts at: 4244 4244
Size: 6115
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3923 276
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SwaI XcmI