Restriction map for
pFN21AB8201
(Circular) (Six-base) MAPSORT of: pFN21AB8201.seq Check: 3835 from: 1 to: 5314
pFN21AB8201 5314 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3124 278
Size: 53 83 186 936 1588 2468
Fragments arranged by size:
2468 1588 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 2657 1937
Size: 328 392 4594
Fragments arranged by size:
4594 392 328
Acc65I G'GTAC_C
Cuts at: 2635 2635
Size: 5314
AclI AA'CG_TT
Cuts at: 1563 3441 3814 1563
Size: 1878 373 3063
Fragments arranged by size:
3063 1878 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3377 4761 5029 1632
Size: 1745 1384 268 1917
Fragments arranged by size:
1917 1745 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5117
AflIII A'CryG_T
Cuts at: 1203 4213 5190 1203
Size: 3010 977 1327
Fragments arranged by size:
3010 1327 977
AgeI A'CCGG_T
Cuts at: 4145 4145
Size: 5314
AhdI GACnn_n'nnGTC
Cuts at: 4043 4043
Size: 5314
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5314
AlwNI CAG_nnn'CTG
Cuts at: 3057 4629 3057
Size: 1572 3742
ApaLI G'TGCA_C
Cuts at: 3371 4527 3371
Size: 1156 4158
ApoI r'AATT_y
Cuts at: 1493 2623 2849 3001 5110 1493
Size: 1130 226 152 2109 1697
Fragments arranged by size:
2109 1697 1130 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3868 160
Size: 872 2836 1606
Fragments arranged by size:
2836 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2424 2502 2639 1870
Size: 73 110 371 78 137 4545
Fragments arranged by size:
4545 371 137 110 78 73
BamHI G'GATC_C
Cuts at: 2644 3011 2644
Size: 367 4947
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2635 4090 618
Size: 325 217 627 848 1455 1842
Fragments arranged by size:
1842 1455 848 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2633 1391
Size: 1242 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2438 2472 3505 3539 1903
Size: 34 501 34 1033 34 3678
Fragments arranged by size:
3678 1033 501 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3206 4416 3206
Size: 1210 4104
BclI T'GATC_A
Cuts at: 1548 2076 1548
Size: 528 4786
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3925 136
Size: 107 122 71 631 605 2253 1525
Fragments arranged by size:
2253 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5309 5309
Size: 5314
BlpI GC'TnA_GC
Cuts at: 2731 2731
Size: 5314
Bme1580I G_kGCm'C
Cuts at: 1790 3375 4531 1790
Size: 1585 1156 2573
Fragments arranged by size:
2573 1585 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2553 3045 4003
Size: 416 53 83 399 24 1136 492 958
Cuts at: 4003 442
Size: 1753
Fragments arranged by size:
1753 1136 958 492 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5314
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 5314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3974 1239
Size: 2735 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 3439 4304 4602 2501
Size: 938 865 298 3213
Fragments arranged by size:
3213 938 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3977 882
Size: 617 2478 2219
Fragments arranged by size:
2478 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3105 493
Size: 711 1901 2702
Fragments arranged by size:
2702 1901 711
BsaBI GATnn'nnATC
Cuts at: 5308 5308
Size: 5314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3121 3503 4138 275
Size: 53 83 186 936 1588 382 635 1451
Fragments arranged by size:
1588 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3747 4145 4419 4566 4977 3747
Size: 398 274 147 411 4084
Fragments arranged by size:
4084 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2588 2618 1178
Size: 30 165 30 1185 30 3874
Fragments arranged by size:
3874 1185 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5314
BseYI C'CCAG_C
Cuts at: 2339 2359 4517 5013 2339
Size: 20 2158 496 2640
Fragments arranged by size:
2640 2158 496 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 1163
Size: 573 681 4060
Fragments arranged by size:
4060 681 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2764 3525 4553 1540
Size: 275 186 763 761 1028 2301
Fragments arranged by size:
2301 1028 763 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2606 3375 3460 4531 2606
Size: 769 85 1071 3389
Fragments arranged by size:
3389 1071 769 85
BsmI GAATG_Cn'
Cuts at: 2335 2825 2918 2335
Size: 490 93 4731
Fragments arranged by size:
4731 490 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2606 2633 3375 3460 4531 1391
Size: 399 816 27 742 85 1071 2174
Fragments arranged by size:
2174 1071 816 742 399 85 27
BspEI T'CCGG_A
Cuts at: 4977 4977
Size: 5314
BspHI T'CATG_A
Cuts at: 3203 4933 3203
Size: 1730 3584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2669 844
Size: 1825 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2209 3201 1394
Size: 429 386 992 3507
Fragments arranged by size:
3507 992 429 386
BsrDI GCAATG_nn'
Cuts at: 65 1248 3809 3983 65
Size: 1183 2561 174 1396
Fragments arranged by size:
2561 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3958 4145 1700
Size: 110 2148 187 2869
Fragments arranged by size:
2869 2148 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5314
BssSI C'ACGA_G
Cuts at: 3374 4386 3374
Size: 1012 4302
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5314
BstBI TT'CG_AA
Cuts at: 2448 4118 2448
Size: 1670 3644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5314
BstYI r'GATC_y
Cuts at: 1972 2142 2644 3011 3396 3413 4865 4951 4963
Size: 170 502 367 385 17 1452 86 12
Cuts at: 4963 5309 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 502 385 367 346 170 86
17 12
BstZ17I GTA'TAC
Cuts at: 2266 2266
Size: 5314
BtgI C'CryG_G
Cuts at: 513 1065 2004 2239 2247 513
Size: 552 939 235 8 3580
Fragments arranged by size:
3580 939 552 235 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4575
Fragments arranged by size:
4575 683 56
BtsI GCAGTG_nn'
Cuts at: 2830 3624 3644 2830
Size: 794 20 4500
Fragments arranged by size:
4500 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5279
DraI TTT'AAA
Cuts at: 2618 2618
Size: 5314
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4321 809
Size: 507 3005 1802
Fragments arranged by size:
3005 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2200 2761
Size: 54 1425 50 275 18 177 193 561
Cuts at: 2761 3650 5279 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 561 275 193 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2761 1537
Size: 275 949 4090
Fragments arranged by size:
4090 949 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 3244 1353
Size: 680 1211 3423
Fragments arranged by size:
3423 1211 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3901 4275 4421 387
Size: 1064 138 2312 374 146 1280
Fragments arranged by size:
2312 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3377 3974 4761 5029 1239
Size: 393 1745 597 787 268 1524
Fragments arranged by size:
1745 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2623 3001 2623
Size: 378 4936
FspI TGC'GCA
Cuts at: 3820 3820
Size: 5314
HaeII r_GCGC'y
Cuts at: 1678 2353 4461 1678
Size: 675 2108 2531
Fragments arranged by size:
2531 2108 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 3955 3987 4029
Size: 32 135 32 698 32 1653 32 42
Cuts at: 4029 4061 1373
Size: 32 2626
Fragments arranged by size:
2626 1653 698 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2658 2904 669
Size: 988 281 720 246 3079
Fragments arranged by size:
3079 988 720 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5314
HpaI GTT'AAC
Cuts at: 2904 2904
Size: 5314
KpnI G_GTAC'C
Cuts at: 2639 2639
Size: 5314
MfeI C'AATT_G
Cuts at: 2913 2913
Size: 5314
MluI A'CGCG_T
Cuts at: 5190 5190
Size: 5314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4428 4612 1305
Size: 3123 184 2007
Fragments arranged by size:
3123 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2202 10
Size: 54 1425 343 177 193 3122
Fragments arranged by size:
3122 1425 343 193 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3272 3631 3790 518
Size: 648 2106 359 159 2042
Fragments arranged by size:
2106 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2730 3407 4555 4800 1606
Size: 1124 677 1148 245 2120
Fragments arranged by size:
2120 1148 1124 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2247 513
Size: 552 939 243 3580
Fragments arranged by size:
3580 939 552 243
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5314
NotI GC'GGCC_GC
Cuts at: 2761 2761
Size: 5314
NspI r_CATG'y
Cuts at: 1132 1229 2672 4217 1132
Size: 97 1443 1545 2229
Fragments arranged by size:
2229 1545 1443 97
PasI CC'CwG_GG
Cuts at: 1348 2608 1348
Size: 1260 4054
PciI A'CATG_T
Cuts at: 4213 4213
Size: 5314
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5314
PmeI GTTT'AAAC
Cuts at: 2618 2618
Size: 5314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 4896 4928 3383
Size: 32 1481 32 3769
Fragments arranged by size:
3769 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5314
PsiI TTA'TAA
Cuts at: 2884 2884
Size: 5314
PstI C_TGCA'G
Cuts at: 830 2666 830
Size: 1836 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5314
SalI G'TCGA_C
Cuts at: 1936 2656 1936
Size: 720 4594
SbfI CC_TGCA'GG
Cuts at: 2666 2666
Size: 5314
ScaI AGT'ACT
Cuts at: 2563 3562 5274 2563
Size: 999 1712 2603
Fragments arranged by size:
2603 1712 999
SfcI C'TryA_G
Cuts at: 826 1045 2662 3797 4478 4669 826
Size: 219 1617 1135 681 191 1471
Fragments arranged by size:
1617 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5314
SmaI CCC'GGG
Cuts at: 1872 2504 2641 1872
Size: 632 137 4545
Fragments arranged by size:
4545 632 137
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2424 2516 3418 4319 4581 820
Size: 197 926 481 92 902 901 262 1553
Fragments arranged by size:
1553 926 902 901 481 262 197 92
SnaBI TAC'GTA
Cuts at: 493 3105 493
Size: 2612 2702
SpeI A'CTAG_T
Cuts at: 152 2453 152
Size: 2301 3013
SphI G_CATG'C
Cuts at: 1132 2672 1132
Size: 1540 3774
SspI AAT'ATT
Cuts at: 5 52 2299 3238 5
Size: 47 2247 939 2081
Fragments arranged by size:
2247 2081 939 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5314
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 2753 513
Size: 552 939 243 506 3074
Fragments arranged by size:
3074 939 552 506 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3341 3358 3511 3696 4141 325
Size: 1255 1761 17 153 185 445 1498
Fragments arranged by size:
1761 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2561 3560 5272
Size: 274 80 33 51 1443 584 999 1712
Cuts at: 5272 96
Size: 138
Fragments arranged by size:
1712 1443 999 584 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2356 2388 5274 5306 2356
Size: 32 2886 32 2364
Fragments arranged by size:
2886 2364 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5314
XbaI T'CTAG_A
Cuts at: 2650 2650
Size: 5314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2473 2473
Size: 5314
XhoI C'TCGA_G
Cuts at: 1943 2424 1943
Size: 481 4833
XmaI C'CCGG_G
Cuts at: 1870 2502 2639 1870
Size: 632 137 4545
Fragments arranged by size:
4545 632 137
XmnI GAAnn'nnTTC
Cuts at: 2288 3443 2288
Size: 1155 4159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3122 276
Size: 53 83 186 936 1588 2468
Fragments arranged by size:
2468 1588 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI