Restriction map for
pFN21AB8200
(Circular) (Six-base) MAPSORT of: pFN21AB8200.seq Check: 8876 from: 1 to: 5821
pFN21AB8200 5821 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2155 2155
Size: 5821
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 3884 5268 5536 1632
Size: 635 1617 1384 268 1917
Fragments arranged by size:
1917 1617 1384 635 268
AfeI AGC'GCT
Cuts at: 2906 2906
Size: 5821
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 2235 4720 5697 1203
Size: 1032 2485 977 1327
Fragments arranged by size:
2485 1327 1032 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2573 2605 2573
Size: 32 5789
AlwNI CAG_nnn'CTG
Cuts at: 2607 2917 3564 5136 2607
Size: 310 647 1572 3292
Fragments arranged by size:
3292 1572 647 310
ApaI G_GGCC'C
Cuts at: 2188 2320 2188
Size: 132 5689
ApaLI G'TGCA_C
Cuts at: 2138 3878 5034 2138
Size: 1740 1156 2925
Fragments arranged by size:
2925 1740 1156
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2189 3146 1870
Size: 73 246 957 4545
Fragments arranged by size:
4545 957 246 73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2454 2545 3142 4597
Size: 325 217 627 333 334 91 597 1455
Cuts at: 4597 618
Size: 1842
Fragments arranged by size:
1842 1455 627 597 334 333 325 217
91
BanII G_rGCy'C
Cuts at: 1391 2034 2148 2188 2320 2564 2879 2936 3140
Size: 643 114 40 132 244 315 57 204
Cuts at: 3140 1391
Size: 4072
Fragments arranged by size:
4072 643 315 244 204 132 114 57
40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4850
BbvCI CC'TCA_GC
Cuts at: 2441 2441
Size: 5821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4432 136
Size: 107 122 71 631 605 2760 1525
Fragments arranged by size:
2760 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2997 5816 2997
Size: 2819 3002
BlpI GC'TnA_GC
Cuts at: 2840 3008 3238 2840
Size: 168 230 5423
Fragments arranged by size:
5423 230 168
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2188 2320 3882 5038 1790
Size: 352 46 132 1562 1156 2573
Fragments arranged by size:
2573 1562 1156 352 132 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2191 3552 4510
Size: 416 53 83 399 24 774 1361 958
Cuts at: 4510 442
Size: 1753
Fragments arranged by size:
1753 1361 958 774 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4481 1239
Size: 3242 2579
Bpu10I CC'TnA_GC
Cuts at: 2441 2868 2441
Size: 427 5394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2132 3095 3946 4811 5109 2132
Size: 963 851 865 298 2844
Fragments arranged by size:
2844 963 865 851 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2236 3612 493
Size: 711 1032 1376 2702
Fragments arranged by size:
2702 1376 1032 711
BsaBI GATnn'nnATC
Cuts at: 2075 2366 5815 2075
Size: 291 3449 2081
Fragments arranged by size:
3449 2081 291
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2625 2823 3628 4010
Size: 53 83 186 936 1092 198 805 382
Cuts at: 4010 4645 275
Size: 635 1451
Fragments arranged by size:
1451 1092 936 805 635 382 198 186
83 53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5596
Fragments arranged by size:
5596 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5821
BseYI C'CCAG_C
Cuts at: 2313 2613 5024 5520 2313
Size: 300 2411 496 2614
Fragments arranged by size:
2614 2411 496 300
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2960 3025 1163
Size: 573 1224 65 3959
Fragments arranged by size:
3959 1224 573 65
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2543 3271 4032 5060 1540
Size: 275 186 542 728 761 1028 2301
Fragments arranged by size:
2301 1028 761 728 542 275 186
BsiHKAI G_wGCw'C
Cuts at: 2142 2148 2157 2879 2936 3009 3032 3882 3967
Size: 6 9 722 57 73 23 850 85
Cuts at: 3967 5038 2142
Size: 1071 2925
Fragments arranged by size:
2925 1071 850 722 85 73 57 23
9 6
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2142 2148 2157 2188 2320 2564
Size: 399 244 108 6 9 31 132 244
Cuts at: 2564 2879 2936 3009 3032 3140 3882 3967 5038
Size: 315 57 73 23 108 742 85 1071
Cuts at: 5038 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 315 244 244 132
108 108 85 73 57 31 23 9
6
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2155 2219 3176 844
Size: 1311 64 957 3489
Fragments arranged by size:
3489 1311 957 64
BsrBI CCG'CTC
Cuts at: 1394 1823 2118 2430 3708 1394
Size: 429 295 312 1278 3507
Fragments arranged by size:
3507 1278 429 312 295
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2480 2542 4465 4652 1700
Size: 110 670 62 1923 187 2869
Fragments arranged by size:
2869 1923 670 187 110 62
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 2141 2355 3881 4893 2141
Size: 214 1526 1012 3069
Fragments arranged by size:
3069 1526 1012 214
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2607 2682 794
Size: 1813 75 3933
Fragments arranged by size:
3933 1813 75
BstBI TT'CG_AA
Cuts at: 2592 4625 2592
Size: 2033 3788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstXI CCAn_nnnn'nTGG
Cuts at: 3052 3052
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 2505 2997 3151 3518 3903 3920 5372 5458
Size: 533 492 154 367 385 17 1452 86
Cuts at: 5458 5470 5816 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 533 492 385 367 346 154
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2555 2829 513
Size: 552 939 551 274 3505
Fragments arranged by size:
3505 939 552 551 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2741 3337 4131 4151 2741
Size: 596 794 20 4411
Fragments arranged by size:
4411 794 596 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DraIII CAC_nnn'GTG
Cuts at: 2556 2556
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2678 4828 809
Size: 507 1362 2150 1802
Fragments arranged by size:
2150 1802 1362 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2540 2709 3268
Size: 54 1425 50 275 18 710 169 559
Cuts at: 3268 4157 5786 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 710 559 275 169 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2540 3268 1537
Size: 275 728 728 4090
Fragments arranged by size:
4090 728 728 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3077 3751 1353
Size: 1724 674 3423
Fragments arranged by size:
3423 1724 674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2877 2934 2146
Size: 731 57 5033
Fragments arranged by size:
5033 731 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 3884 4481 5268 5536 1239
Size: 393 635 1617 597 787 268 1524
Fragments arranged by size:
1617 1524 787 635 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2185 2317 3106 2185
Size: 132 789 4900
Fragments arranged by size:
4900 789 132
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 5821
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2753 2908 4968 1678
Size: 1075 155 2060 2531
Fragments arranged by size:
2531 2060 1075 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4462 4494 4536 4568 1373
Size: 32 135 32 2890 32 42 32 2626
Fragments arranged by size:
2890 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3165 3411 669
Size: 988 281 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2483 4935 5119 1305
Size: 1178 2452 184 2007
Fragments arranged by size:
2452 2007 1178 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2106 3050 3779 4138 4297 518
Size: 648 940 944 729 359 159 2042
Fragments arranged by size:
2042 944 940 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2040 2304 2349 2643 2786 2831 3012 3237
Size: 434 264 45 294 143 45 181 225
Cuts at: 3237 3914 5062 5307 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 434 294 264 245 225
181 143 45 45
NaeI GCC'GGC
Cuts at: 2482 2544 2482
Size: 62 5759
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4330
Fragments arranged by size:
4330 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2480 2542 2480
Size: 62 5759
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NruI TCG'CGA
Cuts at: 2717 2717
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 3179 4724 1132
Size: 97 1950 1545 2229
Fragments arranged by size:
2229 1950 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2556 2917 1108
Size: 1448 361 4012
Fragments arranged by size:
4012 1448 361
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3106 3106
Size: 5821
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PspOMI G'GGCC_C
Cuts at: 2184 2316 2184
Size: 132 5689
PstI C_TGCA'G
Cuts at: 830 3173 830
Size: 2343 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 2040 2040
Size: 5821
SacI G_AGCT'C
Cuts at: 2148 2879 2936 2148
Size: 731 57 5033
Fragments arranged by size:
5033 731 57
SacII CC_GC'GG
Cuts at: 2832 2832
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SapI GCTCTTCn'nnn_
Cuts at: 3077 3077
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 3169 4304 4985 5176 826
Size: 219 2124 1135 681 191 1471
Fragments arranged by size:
2124 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 3110 3925 4826 5088 820
Size: 197 926 204 963 815 901 262 1553
Fragments arranged by size:
1553 963 926 901 815 262 204 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 3260 513
Size: 552 939 129 1127 3074
Fragments arranged by size:
3074 1127 939 552 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2081 2563 3848 3865 4018 4203 4648
Size: 1255 501 482 1285 17 153 185 445
Cuts at: 4648 325
Size: 1498
Fragments arranged by size:
1498 1285 1255 501 482 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4067 5779 96
Size: 274 80 33 51 1443 2090 1712 138
Fragments arranged by size:
2090 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5781 5813 5781
Size: 32 5789
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI