Restriction map for
pFN21AB8196
(Circular) (Six-base) MAPSORT of: pFN21AB8196.seq Check: 999 from: 1 to: 6079
pFN21AB8196 6079 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2488 2488
Size: 6079
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2126 2225 3889 278
Size: 53 83 186 936 590 99 1664 2468
Fragments arranged by size:
2468 1664 936 590 186 99 83 53
AccI GT'mk_AC
Cuts at: 1937 3422 1937
Size: 1485 4594
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3297 4142 5526 5794 1632
Size: 1665 845 1384 268 1917
Fragments arranged by size:
1917 1665 1384 845 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 2646 4978 5955 1203
Size: 1443 2332 977 1327
Fragments arranged by size:
2332 1443 1327 977
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 2169 2235 3111 4808 2169
Size: 66 876 1697 3440
Fragments arranged by size:
3440 1697 876 66
AleI CACnn'nnGTG
Cuts at: 1166 2669 1166
Size: 1503 4576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6079
AlwNI CAG_nnn'CTG
Cuts at: 2175 2480 3822 5394 2175
Size: 305 1342 1572 2860
Fragments arranged by size:
2860 1572 1342 305
ApaI G_GGCC'C
Cuts at: 2116 2149 2182 2116
Size: 33 33 6013
Fragments arranged by size:
6013 33 33
ApaLI G'TGCA_C
Cuts at: 4136 5292 4136
Size: 1156 4923
ApoI r'AATT_y
Cuts at: 1493 3049 3388 3614 3766 5875 1493
Size: 1556 339 226 152 2109 1697
Fragments arranged by size:
2109 1697 1556 339 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 2142 2148 2208 2233 2241 2280 3404
Size: 73 199 6 60 25 8 39 1124
Cuts at: 3404 1870
Size: 4545
Fragments arranged by size:
4545 1124 199 73 60 39 25 8
6
BamHI G'GATC_C
Cuts at: 2674 3409 3776 2674
Size: 735 367 4977
Fragments arranged by size:
4977 735 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2511 3400 4855 618
Size: 325 217 627 724 889 1455 1842
Fragments arranged by size:
1842 1455 889 724 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2116 2149 2182 2580 3203 3398 1391
Size: 725 33 33 398 623 195 4072
Fragments arranged by size:
4072 725 623 398 195 33 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2477 3008 928
Size: 971 578 531 3999
Fragments arranged by size:
3999 971 578 531
BbvCI CC'TCA_GC
Cuts at: 2699 2699
Size: 6079
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4270 4304 1903
Size: 34 2333 34 3678
Fragments arranged by size:
3678 2333 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4690 136
Size: 107 122 71 631 605 3018 1525
Fragments arranged by size:
3018 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 2636 2746 2838 3496 2636
Size: 110 92 658 5219
Fragments arranged by size:
5219 658 110 92
Bme1580I G_kGCm'C
Cuts at: 1790 2116 2149 2182 2248 4140 5296 1790
Size: 326 33 33 66 1892 1156 2573
Fragments arranged by size:
2573 1892 1156 326 66 33 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2163 3304 3810
Size: 416 53 83 399 24 746 1141 506
Cuts at: 3810 4768 442
Size: 958 1753
Fragments arranged by size:
1753 1141 958 746 506 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2059 2943 4739 1239
Size: 820 884 1796 2579
Fragments arranged by size:
2579 1796 884 820
Bpu10I CC'TnA_GC
Cuts at: 2551 2699 2551
Size: 148 5931
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4204 5069 5367 4204
Size: 865 298 4916
Fragments arranged by size:
4916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2080 2847 4742 882
Size: 617 581 767 1895 2219
Fragments arranged by size:
2219 1895 767 617 581
BsaAI yAC'GTr
Cuts at: 493 1204 2649 3870 493
Size: 711 1445 1221 2702
Fragments arranged by size:
2702 1445 1221 711
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 2222 3886 4268
Size: 53 83 186 936 590 99 1664 382
Cuts at: 4268 4903 275
Size: 635 1451
Fragments arranged by size:
1664 1451 936 635 590 382 186 99
83 53
BsaWI w'CCGG_w
Cuts at: 3059 4512 4910 5184 5331 5742 3059
Size: 1453 398 274 147 411 3396
Fragments arranged by size:
3396 1453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2077 2107 1178
Size: 30 165 30 674 30 5150
Fragments arranged by size:
5150 674 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 3046 1186
Size: 912 948 4219
Fragments arranged by size:
4219 948 912
BseYI C'CCAG_C
Cuts at: 2076 2335 2342 3075 3116 5282 5778 2076
Size: 259 7 733 41 2166 496 2377
Fragments arranged by size:
2377 2166 733 496 259 41 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2052 3192 3341 1163
Size: 573 316 1140 149 3901
Fragments arranged by size:
3901 1140 573 316 149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2394 3529 4290 5318 1540
Size: 275 186 393 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 393 275 186
BsiHKAI G_wGCw'C
Cuts at: 4140 4225 5296 4140
Size: 85 1071 4923
Fragments arranged by size:
4923 1071 85
BsmI GAATG_Cn'
Cuts at: 2070 3590 3683 2070
Size: 1520 93 4466
Fragments arranged by size:
4466 1520 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2149 2182 2248 2580 3203 3398
Size: 399 326 33 33 66 332 623 195
Cuts at: 3398 4140 4225 5296 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 623 399 332 326 195
85 66 33 33
BspEI T'CCGG_A
Cuts at: 3059 5742 3059
Size: 2683 3396
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 3434 844
Size: 1644 946 3489
Fragments arranged by size:
3489 1644 946
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4574 4748 65
Size: 1183 3326 174 1396
Fragments arranged by size:
3326 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2508 4723 4910 1700
Size: 110 698 2215 187 2869
Fragments arranged by size:
2869 2215 698 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6079
BssHII G'CGCG_C
Cuts at: 1925 2314 1925
Size: 389 5690
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 3298 4883 3298
Size: 1585 4494
BstEII G'GTnAC_C
Cuts at: 1184 2260 1184
Size: 1076 5003
BstXI CCAn_nnnn'nTGG
Cuts at: 2857 2857
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 2499 2674 3324 3409 3776 4161 4178 5630
Size: 527 175 650 85 367 385 17 1452
Cuts at: 5630 5716 5728 6074 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 650 527 385 367 346 175
86 85 17 12
Bsu36I CC'TnA_GG
Cuts at: 3366 3366
Size: 6079
BtgI C'CryG_G
Cuts at: 513 1065 2004 2484 2658 2679 2781 513
Size: 552 939 480 174 21 102 3811
Fragments arranged by size:
3811 939 552 480 174 102 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2437 2674 487
Size: 683 56 1211 237 3892
Fragments arranged by size:
3892 1211 683 237 56
BtsI GCAGTG_nn'
Cuts at: 2358 2798 3324 3595 4389 4409 2358
Size: 440 526 271 794 20 4028
Fragments arranged by size:
4028 794 526 440 271 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2391 3246 3526
Size: 54 1425 50 275 18 561 855 280
Cuts at: 3526 4415 6044 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 855 561 280 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2391 3526 1537
Size: 275 579 1135 4090
Fragments arranged by size:
4090 1135 579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4009 1353
Size: 2656 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4666 5040 5186 387
Size: 1064 138 3077 374 146 1280
Fragments arranged by size:
3077 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2943 3297 4142 4739 5526 5794
Size: 393 427 884 354 845 597 787 268
Cuts at: 5794 1239
Size: 1524
Fragments arranged by size:
1524 884 845 787 597 427 393 354
268
EcoO109I rG'GnC_Cy
Cuts at: 2113 2163 2229 2237 2277 2384 2533 2927 3072
Size: 50 66 8 40 107 149 394 145
Cuts at: 3072 2113
Size: 5120
Fragments arranged by size:
5120 394 149 145 107 66 50 40
8
EcoRI G'AATT_C
Cuts at: 3049 3388 3766 3049
Size: 339 378 5362
Fragments arranged by size:
5362 378 339
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 2336 2343 3085 5226 1678
Size: 658 7 742 2141 2531
Fragments arranged by size:
2531 2141 742 658 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2155 2187 2221 2253 3097
Size: 32 135 32 583 32 34 32 844
Cuts at: 3097 3129 4720 4752 4794 4826 1373
Size: 32 1591 32 42 32 2626
Fragments arranged by size:
2626 1591 844 583 135 42 34 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2938 3423 3669 669
Size: 988 281 1000 485 246 3079
Fragments arranged by size:
3079 1000 988 485 281 246
HindIII A'AGCT_T
Cuts at: 748 3151 748
Size: 2403 3676
HpaI GTT'AAC
Cuts at: 2938 3669 2938
Size: 731 5348
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5193 5377 1305
Size: 3888 184 2007
Fragments arranged by size:
3888 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3248 10
Size: 54 1425 343 1416 2841
Fragments arranged by size:
2841 1425 1416 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2669 3092 4037 4396 4555 518
Size: 648 1503 423 945 359 159 2042
Fragments arranged by size:
2042 1503 945 648 423 359 159
MspA1I CmG'CkG
Cuts at: 1606 2669 2750 2984 3079 3495 4172 5320 5565
Size: 1063 81 234 95 416 677 1148 245
Cuts at: 5565 1606
Size: 2120
Fragments arranged by size:
2120 1148 1063 677 416 245 234 95
81
NcoI C'CATG_G
Cuts at: 513 1065 2004 2781 513
Size: 552 939 777 3811
Fragments arranged by size:
3811 939 777 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 2217 3437 4982 1132
Size: 97 988 1220 1545 2229
Fragments arranged by size:
2229 1545 1220 988 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2175 2602 1108
Size: 1067 427 4585
Fragments arranged by size:
4585 1067 427
PfoI T'CCnGG_A
Cuts at: 2502 2502
Size: 6079
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2163 2229 2237 2277 2384 2533 2927 3072 2163
Size: 66 8 40 107 149 394 145 5170
Fragments arranged by size:
5170 394 149 145 107 66 40 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6079
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PspOMI G'GGCC_C
Cuts at: 2112 2145 2178 2112
Size: 33 33 6013
Fragments arranged by size:
6013 33 33
PstI C_TGCA'G
Cuts at: 830 2805 3431 830
Size: 1975 626 3478
Fragments arranged by size:
3478 1975 626
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 2669 2750 2984 2669
Size: 81 234 5764
Fragments arranged by size:
5764 234 81
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SanDI GG'GwC_CC
Cuts at: 2163 2229 2237 3072 2163
Size: 66 8 835 5170
Fragments arranged by size:
5170 835 66 8
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SfcI C'TryA_G
Cuts at: 826 1045 2801 3134 3427 4562 5243 5434 826
Size: 219 1756 333 293 1135 681 191 1471
Fragments arranged by size:
1756 1471 1135 681 333 293 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 2144 2235 2243 2282 3406 1872
Size: 272 91 8 39 1124 4545
Fragments arranged by size:
4545 1124 272 91 39 8
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4183 5084 5346 820
Size: 197 926 2240 901 262 1553
Fragments arranged by size:
2240 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 2217 3437 1132
Size: 1085 1220 3774
Fragments arranged by size:
3774 1220 1085
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 2288 1517
Size: 771 5308
StyI C'CwwG_G
Cuts at: 513 1065 2004 2781 3518 513
Size: 552 939 777 737 3074
Fragments arranged by size:
3074 939 777 737 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4106 4123 4276 4461 4906 325
Size: 1255 2526 17 153 185 445 1498
Fragments arranged by size:
2526 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2442 3175 4325
Size: 274 80 33 51 1443 465 733 1150
Cuts at: 4325 6037 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1150 733 465 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2547 2579 6039 6071 2547
Size: 32 3460 32 2555
Fragments arranged by size:
3460 2555 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6079
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2596 2734 2596
Size: 138 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 2142 2233 2241 2280 3404 1870
Size: 272 91 8 39 1124 4545
Fragments arranged by size:
4545 1124 272 91 39 8
XmnI GAAnn'nnTTC
Cuts at: 2308 2886 3294 4208 2308
Size: 578 408 914 4179
Fragments arranged by size:
4179 914 578 408
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2124 2223 3887 276
Size: 53 83 186 936 590 99 1664 2468
Fragments arranged by size:
2468 1664 936 590 186 99 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI