Restriction map for
pFN21AB8189
(Circular) (Six-base) MAPSORT of: pFN21AB8189.seq Check: 1381 from: 1 to: 6043
pFN21AB8189 6043 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3853 278
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3386 1937
Size: 1449 4594
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2334 2625 2698 2782 2866 2950 3034 3202
Size: 702 291 73 84 84 84 84 168
Cuts at: 3202 4106 5490 5758 1632
Size: 904 1384 268 1917
Fragments arranged by size:
1917 1384 904 702 291 268 168 84
84 84 84 73
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 4942 5919 1203
Size: 3739 977 1327
Fragments arranged by size:
3739 1327 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 2925 1166
Size: 1759 4284
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6043
AlwNI CAG_nnn'CTG
Cuts at: 2047 2152 3786 5358 2047
Size: 105 1634 1572 2732
Fragments arranged by size:
2732 1634 1572 105
ApaLI G'TGCA_C
Cuts at: 4100 5256 4100
Size: 1156 4887
ApoI r'AATT_y
Cuts at: 1493 2307 2554 3252 3352 3578 3730 5839 1493
Size: 814 247 698 100 226 152 2109 1697
Fragments arranged by size:
2109 1697 814 698 247 226 152 100
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 3368 1870
Size: 73 1425 4545
Fragments arranged by size:
4545 1425 73
BamHI G'GATC_C
Cuts at: 2832 3373 3740 2832
Size: 541 367 5135
Fragments arranged by size:
5135 541 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3364 4819 618
Size: 325 217 627 1577 1455 1842
Fragments arranged by size:
1842 1577 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2726 2894 2978 3062 3362 1391
Size: 1335 168 84 84 300 4072
Fragments arranged by size:
4072 1335 300 168 84 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2322 2882 928
Size: 971 423 560 4089
Fragments arranged by size:
4089 971 560 423
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4234 4268 1903
Size: 34 2297 34 3678
Fragments arranged by size:
3678 2297 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3935 5145 3935
Size: 1210 4833
BclI T'GATC_A
Cuts at: 1548 2299 1548
Size: 751 5292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4654 136
Size: 107 122 71 631 605 2982 1525
Fragments arranged by size:
2982 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6038 6038
Size: 6043
BlpI GC'TnA_GC
Cuts at: 3460 3460
Size: 6043
Bme1580I G_kGCm'C
Cuts at: 1790 4104 5260 1790
Size: 2314 1156 2573
Fragments arranged by size:
2573 2314 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6043
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2351 2682 2766
Size: 416 53 83 399 24 934 331 84
Cuts at: 2766 3774 4732 442
Size: 1008 958 1753
Fragments arranged by size:
1753 1008 958 934 416 399 331 84
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 2457 2908 3030 3114 3282 4703 1239
Size: 1028 190 451 122 84 168 1421 2579
Fragments arranged by size:
2579 1421 1028 451 190 168 122 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 4168 5033 5331 2360
Size: 1808 865 298 3072
Fragments arranged by size:
3072 1808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2276 2378 4706 882
Size: 617 777 102 2328 2219
Fragments arranged by size:
2328 2219 777 617 102
BsaAI yAC'GTr
Cuts at: 493 1204 3834 493
Size: 711 2630 2702
Fragments arranged by size:
2702 2630 711
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3850 4232 4867 275
Size: 53 83 186 936 2317 382 635 1451
Fragments arranged by size:
2317 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2187 4476 4874 5148 5295 5706 2187
Size: 2289 398 274 147 411 2524
Fragments arranged by size:
2524 2289 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5818
Fragments arranged by size:
5818 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2501 1186
Size: 1315 4728
BseYI C'CCAG_C
Cuts at: 2422 5246 5742 2422
Size: 2824 496 2723
Fragments arranged by size:
2824 2723 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5470
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3318 3493 4254 5282 1540
Size: 275 186 1317 175 761 1028 2301
Fragments arranged by size:
2301 1317 1028 761 275 186 175
BsiHKAI G_wGCw'C
Cuts at: 2062 2726 2894 2978 3062 4104 4189 5260 2062
Size: 664 168 84 84 1042 85 1071 2845
Fragments arranged by size:
2845 1071 1042 664 168 85 84 84
BsmI GAATG_Cn'
Cuts at: 3554 3647 3554
Size: 93 5950
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2726 2894 2978 3062 3362 4104
Size: 399 272 664 168 84 84 300 742
Cuts at: 4104 4189 5260 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 664 399 300 272 168
85 84 84
BspEI T'CCGG_A
Cuts at: 2187 5706 2187
Size: 3519 2524
BspHI T'CATG_A
Cuts at: 3932 5662 3932
Size: 1730 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2221 3398 844
Size: 1377 1177 3489
Fragments arranged by size:
3489 1377 1177
BsrBI CCG'CTC
Cuts at: 1394 1823 3930 1394
Size: 429 2107 3507
Fragments arranged by size:
3507 2107 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2953 4538 4712 65
Size: 1183 1705 1585 174 1396
Fragments arranged by size:
1705 1585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4687 4874 1700
Size: 110 2877 187 2869
Fragments arranged by size:
2877 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6043
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6043
BssSI C'ACGA_G
Cuts at: 4103 5115 4103
Size: 1012 5031
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6043
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 2155 1184
Size: 971 5072
BstYI r'GATC_y
Cuts at: 1972 2832 3373 3740 4125 4142 5594 5680 5692
Size: 860 541 367 385 17 1452 86 12
Cuts at: 5692 6038 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 860 541 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2040 2040
Size: 6043
BtgI C'CryG_G
Cuts at: 513 1065 2004 2586 3339 513
Size: 552 939 582 753 3217
Fragments arranged by size:
3217 939 753 582 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5304
Fragments arranged by size:
5304 683 56
BtsI GCAGTG_nn'
Cuts at: 3559 4353 4373 3559
Size: 794 20 5229
Fragments arranged by size:
5229 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2935 2970 529
Size: 35 2371 35 3602
Fragments arranged by size:
3602 2371 35 35
DraI TTT'AAA
Cuts at: 2383 3347 2383
Size: 964 5079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5050 809
Size: 507 3734 1802
Fragments arranged by size:
3734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3490 4379 6008
Size: 54 1425 50 275 18 1660 889 1629
Cuts at: 6008 8
Size: 43
Fragments arranged by size:
1660 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3490 1537
Size: 275 1678 4090
Fragments arranged by size:
4090 1678 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 3973 1353
Size: 714 1906 3423
Fragments arranged by size:
3423 1906 714
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4630 5004 5150 387
Size: 1064 138 3041 374 146 1280
Fragments arranged by size:
3041 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2724 2892 2976 3060 2724
Size: 168 84 84 5707
Fragments arranged by size:
5707 168 84 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2334 2457 2625 2698 2782 2866
Size: 393 635 67 123 168 73 84 84
Cuts at: 2866 2908 2950 3030 3034 3114 3202 3282 4106
Size: 42 42 80 4 80 88 80 824
Cuts at: 4106 4703 5490 5758 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 824 787 635 597 393 268 168
123 88 84 84 80 80 80 73
67 42 42 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2968 2968
Size: 6043
EcoO109I rG'GnC_Cy
Cuts at: 2044 2224 2335 2044
Size: 180 111 5752
Fragments arranged by size:
5752 180 111
EcoRI G'AATT_C
Cuts at: 2554 3252 3352 3730 2554
Size: 698 100 378 4867
Fragments arranged by size:
4867 698 378 100
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 5190 1678
Size: 3512 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2309 2341 2617
Size: 32 135 32 440 32 265 32 276
Cuts at: 2617 2649 4684 4716 4758 4790 1373
Size: 32 2035 32 42 32 2626
Fragments arranged by size:
2626 2035 440 276 265 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3387 3633 669
Size: 988 281 1449 246 3079
Fragments arranged by size:
3079 1449 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6043
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2112 2583 5157 5341 1305
Size: 807 471 2574 184 2007
Fragments arranged by size:
2574 2007 807 471 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4221
Fragments arranged by size:
4221 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2607 2925 4001 4360 4519 518
Size: 648 1441 318 1076 359 159 2042
Fragments arranged by size:
2042 1441 1076 648 359 318 159
MspA1I CmG'CkG
Cuts at: 1606 2422 2802 2886 3459 4136 5284 5529 1606
Size: 816 380 84 573 677 1148 245 2120
Fragments arranged by size:
2120 1148 816 677 573 380 245 84
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4552
Fragments arranged by size:
4552 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 3401 4946 1132
Size: 97 2172 1545 2229
Fragments arranged by size:
2229 2172 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2502 1348
Size: 1154 4889
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2152 2468 1108
Size: 1044 316 4683
Fragments arranged by size:
4683 1044 316
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2224 2335 2224
Size: 111 5932
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PstI C_TGCA'G
Cuts at: 830 3395 830
Size: 2565 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2802 2886 2802
Size: 84 5959
SacI G_AGCT'C
Cuts at: 2726 2894 2978 3062 2726
Size: 168 84 84 5707
Fragments arranged by size:
5707 168 84 84
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SapI GCTCTTCn'nnn_
Cuts at: 2067 2067
Size: 6043
SbfI CC_TGCA'GG
Cuts at: 3395 3395
Size: 6043
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SfcI C'TryA_G
Cuts at: 826 1045 2230 2803 3139 3391 4526 5207 5398
Size: 219 1185 573 336 252 1135 681 191
Cuts at: 5398 826
Size: 1471
Fragments arranged by size:
1471 1185 1135 681 573 336 252 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 3370 1872
Size: 1498 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2375 4147 5048 5310 820
Size: 197 926 432 1772 901 262 1553
Fragments arranged by size:
1772 1553 926 901 432 262 197
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 2711 2963 3150 1517
Size: 1194 252 187 4410
Fragments arranged by size:
4410 1194 252 187
StyI C'CwwG_G
Cuts at: 513 1065 2004 3482 513
Size: 552 939 1478 3074
Fragments arranged by size:
3074 1478 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2918 3332 4070 4087 4240 4425 4870
Size: 1255 1338 414 738 17 153 185 445
Cuts at: 4870 325
Size: 1498
Fragments arranged by size:
1498 1338 1255 738 445 414 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 4289 6001
Size: 274 80 33 51 1443 201 2111 1712
Cuts at: 6001 96
Size: 138
Fragments arranged by size:
2111 1712 1443 274 201 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2319 2351 6003 6035 2319
Size: 32 3652 32 2327
Fragments arranged by size:
3652 2327 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6043
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 3368 1870
Size: 1498 4545
XmnI GAAnn'nnTTC
Cuts at: 2264 2543 2711 2795 2879 2963 3047 3215 4172
Size: 279 168 84 84 84 84 168 957
Cuts at: 4172 2264
Size: 4135
Fragments arranged by size:
4135 957 279 168 168 84 84 84
84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3851 276
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI