Restriction map for
pFN21AB8185
(Circular) (Six-base) MAPSORT of: pFN21AB8185.seq Check: 4661 from: 1 to: 6088
pFN21AB8185 6088 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2660 3155 4151 5535 5803 1632
Size: 1028 495 996 1384 268 1917
Fragments arranged by size:
1917 1384 1028 996 495 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 2794 3831 5403 2794
Size: 1037 1572 3479
Fragments arranged by size:
3479 1572 1037
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 3397 3623 3775 5884 1493
Size: 1904 226 152 2109 1697
Fragments arranged by size:
2109 1904 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 3413 1870
Size: 73 1470 4545
Fragments arranged by size:
4545 1470 73
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2565 2738 3409 4864 618
Size: 325 217 627 778 173 671 1455 1842
Fragments arranged by size:
1842 1455 778 671 627 325 217 173
BanII G_rGCy'C
Cuts at: 1391 3407 1391
Size: 2016 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2666 2943 928
Size: 971 767 277 4073
Fragments arranged by size:
4073 971 767 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4279 4313 1903
Size: 34 2342 34 3678
Fragments arranged by size:
3678 2342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2381 4699 136
Size: 107 122 71 631 605 709 2318 1525
Fragments arranged by size:
2318 1525 709 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 2331 2432 2741 4149 5305 1790
Size: 541 101 309 1408 1156 2573
Fragments arranged by size:
2573 1408 1156 541 309 101
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3819 4777 442
Size: 416 53 83 399 24 2402 958 1753
Fragments arranged by size:
2402 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2259 2307 2697 3137 3388 4748 1239
Size: 1020 48 390 440 251 1360 2579
Fragments arranged by size:
2579 1360 1020 440 390 251 48
Bpu10I CC'TnA_GC
Cuts at: 2099 2099
Size: 6088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2558 2633 2651 3299 4213 5078 5376 2558
Size: 75 18 648 914 865 298 3270
Fragments arranged by size:
3270 914 865 648 298 75 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3879 493
Size: 711 2675 2702
Fragments arranged by size:
2702 2675 711
BsaBI GATnn'nnATC
Cuts at: 3167 6082 3167
Size: 2915 3173
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4521 4919 5193 5340 5751 4521
Size: 398 274 147 411 4858
Fragments arranged by size:
4858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2953 2983 1178
Size: 30 165 30 1550 30 4283
Fragments arranged by size:
4283 1550 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6088
BseYI C'CCAG_C
Cuts at: 2330 2394 2557 5291 5787 2330
Size: 64 163 2734 496 2631
Fragments arranged by size:
2734 2631 496 163 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3332 1163
Size: 573 1596 3919
Fragments arranged by size:
3919 1596 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2659 3538 4299 5327 1540
Size: 275 186 658 879 761 1028 2301
Fragments arranged by size:
2301 1028 879 761 658 275 186
BsiHKAI G_wGCw'C
Cuts at: 4149 4234 5305 4149
Size: 85 1071 4932
Fragments arranged by size:
4932 1071 85
BsmI GAATG_Cn'
Cuts at: 3599 3692 3599
Size: 93 5995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2331 2432 2741 3407 4149 4234 5305
Size: 399 541 101 309 666 742 85 1071
Cuts at: 5305 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 666 541 399 309 101
85
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 2868 3977 5707 2868
Size: 1109 1730 3249
Fragments arranged by size:
3249 1730 1109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 844
Size: 2599 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2188 3147 3304 3975 1394
Size: 429 365 959 157 671 3507
Fragments arranged by size:
3507 959 671 429 365 157
BsrDI GCAATG_nn'
Cuts at: 65 1248 2615 2725 4583 4757 65
Size: 1183 1367 110 1858 174 1396
Fragments arranged by size:
1858 1396 1367 1183 174 110
BsrFI r'CCGG_y
Cuts at: 1700 1810 2562 4732 4919 1700
Size: 110 752 2170 187 2869
Fragments arranged by size:
2869 2170 752 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 4148 5160 4148
Size: 1012 5076
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6088
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2094 2205 3120 3418 3785 4170 4187 5639
Size: 122 111 915 298 367 385 17 1452
Cuts at: 5639 5725 5737 6083 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 915 385 367 346 298 122
111 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2579 513
Size: 552 939 575 4022
Fragments arranged by size:
4022 939 575 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 2391 3604 4398 4418 2391
Size: 1213 794 20 4061
Fragments arranged by size:
4061 1213 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DraIII CAC_nnn'GTG
Cuts at: 2902 2902
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2522 5095 809
Size: 507 1206 2573 1802
Fragments arranged by size:
2573 1802 1206 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2119 2656
Size: 54 1425 50 275 18 258 31 537
Cuts at: 2656 2805 3295 3535 4424 6053 8
Size: 149 490 240 889 1629 43
Fragments arranged by size:
1629 1425 889 537 490 275 258 240
149 54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 2656 3535 1537
Size: 275 844 879 4090
Fragments arranged by size:
4090 879 844 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2864 4018 1353
Size: 1511 1154 3423
Fragments arranged by size:
3423 1511 1154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2071 4675 5049 5195 387
Size: 1064 138 435 47 2604 374 146 1280
Fragments arranged by size:
2604 1280 1064 435 374 146 138 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2259 2307 2660 2697 3137 3155 3388
Size: 393 627 48 353 37 440 18 233
Cuts at: 3388 4151 4748 5535 5803 1239
Size: 763 597 787 268 1524
Fragments arranged by size:
1524 787 763 627 597 440 393 353
268 233 48 37 18
EcoO109I rG'GnC_Cy
Cuts at: 2259 3048 2259
Size: 789 5299
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 2983 5235 1678
Size: 1305 2252 2531
Fragments arranged by size:
2531 2252 1305
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2426 2458 2912 2944 3200
Size: 32 135 32 854 32 454 32 256
Cuts at: 3200 3232 4729 4761 4803 4835 1373
Size: 32 1497 32 42 32 2626
Fragments arranged by size:
2626 1497 854 454 256 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2126 748
Size: 1378 4710
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3120 5202 5386 1305
Size: 1815 2082 184 2007
Fragments arranged by size:
2082 2007 1815 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2807 3297 10
Size: 54 1425 343 975 490 2801
Fragments arranged by size:
2801 1425 975 490 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 4046 4405 4564 518
Size: 648 1365 1515 359 159 2042
Fragments arranged by size:
2042 1515 1365 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3504 4181 5329 5574 1606
Size: 1898 677 1148 245 2120
Fragments arranged by size:
2120 1898 1148 677 245
NaeI GCC'GGC
Cuts at: 2564 2564
Size: 6088
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4597
Fragments arranged by size:
4597 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NgoMIV G'CCGG_C
Cuts at: 2562 2562
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 3446 4991 1132
Size: 97 2217 1545 2229
Fragments arranged by size:
2229 2217 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2902 3366 1108
Size: 1794 464 3830
Fragments arranged by size:
3830 1794 464
PfoI T'CCnGG_A
Cuts at: 3114 3114
Size: 6088
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2259 3048 2259
Size: 789 5299
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PstI C_TGCA'G
Cuts at: 830 3228 3440 830
Size: 2398 212 3478
Fragments arranged by size:
3478 2398 212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SanDI GG'GwC_CC
Cuts at: 2259 3048 2259
Size: 789 5299
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 3224 3436 4571 5252 5443 826
Size: 219 2179 212 1135 681 191 1471
Fragments arranged by size:
2179 1471 1135 681 219 212 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2573 2648 2666 3314 4192 5093
Size: 197 926 630 75 18 648 878 901
Cuts at: 5093 5355 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 878 648 630 262 197
75 18
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2004 2916 3073 3360 3527 513
Size: 552 939 912 157 287 167 3074
Fragments arranged by size:
3074 939 912 552 287 167 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2164 4115 4132 4285 4470 4915 325
Size: 1255 584 1951 17 153 185 445 1498
Fragments arranged by size:
1951 1498 1255 584 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2835 3261 4334
Size: 274 80 33 51 1443 858 426 1073
Cuts at: 4334 6046 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1073 858 426 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6048 6080 6048
Size: 32 6056
Tth111I GACn'n_nGTC
Cuts at: 1316 2522 1316
Size: 1206 4882
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 2129 4217 2129
Size: 2088 4000
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI