(Circular) (Six-base) MAPSORT of: pFN21AB8183.seq Check: 5780 from: 1 to: 5317 pFN21AB8183 5317 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3127 278 Size: 53 83 186 936 1591 2468 Fragments arranged by size: 2468 1591 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2660 1937 Size: 723 4594 Acc65I G'GTAC_C Cuts at: 2638 2638 Size: 5317 AclI AA'CG_TT Cuts at: 1563 3444 3817 1563 Size: 1881 373 3063 Fragments arranged by size: 3063 1881 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2427 3380 4764 5032 1632 Size: 795 953 1384 268 1917 Fragments arranged by size: 1917 1384 953 795 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5120 AflIII A'CryG_T Cuts at: 1203 4216 5193 1203 Size: 3013 977 1327 Fragments arranged by size: 3013 1327 977 AgeI A'CCGG_T Cuts at: 4148 4148 Size: 5317 AhdI GACnn_n'nnGTC Cuts at: 4046 4046 Size: 5317 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5317 AlwNI CAG_nnn'CTG Cuts at: 2272 3060 4632 2272 Size: 788 1572 2957 Fragments arranged by size: 2957 1572 788 ApaI G_GGCC'C Cuts at: 2054 2054 Size: 5317 ApaLI G'TGCA_C Cuts at: 3374 4530 3374 Size: 1156 4161 ApoI r'AATT_y Cuts at: 1493 2626 2852 3004 5113 1493 Size: 1133 226 152 2109 1697 Fragments arranged by size: 2109 1697 1133 226 152 AseI AT'TA_AT Cuts at: 160 1032 3871 160 Size: 872 2839 1606 Fragments arranged by size: 2839 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5317 AvaI C'yCGr_G Cuts at: 1870 1943 2053 2102 2642 1870 Size: 73 110 49 540 4545 Fragments arranged by size: 4545 540 110 73 49 BamHI G'GATC_C Cuts at: 2647 3014 2647 Size: 367 4950 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2607 2638 4093 618 Size: 325 217 627 246 574 31 1455 1842 Fragments arranged by size: 1842 1455 627 574 325 246 217 31 BanII G_rGCy'C Cuts at: 1391 2054 2102 2636 1391 Size: 663 48 534 4072 Fragments arranged by size: 4072 663 534 48 BbeI G_GCGC'C Cuts at: 2037 2611 2037 Size: 574 4743 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2406 928 Size: 971 507 3839 Fragments arranged by size: 3839 971 507 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3508 3542 1903 Size: 34 1571 34 3678 Fragments arranged by size: 3678 1571 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3209 4419 3209 Size: 1210 4107 BclI T'GATC_A Cuts at: 1548 1548 Size: 5317 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3928 136 Size: 107 122 71 631 605 2256 1525 Fragments arranged by size: 2256 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5312 5312 Size: 5317 BlpI GC'TnA_GC Cuts at: 2734 2734 Size: 5317 Bme1580I G_kGCm'C Cuts at: 1790 2054 3378 4534 1790 Size: 264 1324 1156 2573 Fragments arranged by size: 2573 1324 1156 264 BmgBI CAC'GTC Cuts at: 1318 2404 1318 Size: 1086 4231 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2586 3048 4006 Size: 416 53 83 399 24 1169 462 958 Cuts at: 4006 442 Size: 1753 Fragments arranged by size: 1753 1169 958 462 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5317 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2512 2512 Size: 5317 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3977 1239 Size: 2738 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3442 4307 4605 3442 Size: 865 298 4154 Fragments arranged by size: 4154 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3980 882 Size: 617 2481 2219 Fragments arranged by size: 2481 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3108 493 Size: 711 1904 2702 Fragments arranged by size: 2702 1904 711 BsaBI GATnn'nnATC Cuts at: 5311 5311 Size: 5317 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 2608 3124 3506 Size: 53 83 186 936 501 574 516 382 Cuts at: 3506 4141 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 574 516 501 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3750 4148 4422 4569 4980 3750 Size: 398 274 147 411 4087 Fragments arranged by size: 4087 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5092 Fragments arranged by size: 5092 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2390 2402 2488 1186 Size: 910 294 12 86 4015 Fragments arranged by size: 4015 910 294 86 12 BseYI C'CCAG_C Cuts at: 2134 4520 5016 2134 Size: 2386 496 2435 Fragments arranged by size: 2435 2386 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4744 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2767 3528 4556 1540 Size: 275 186 766 761 1028 2301 Fragments arranged by size: 2301 1028 766 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2252 3378 3463 4534 2252 Size: 1126 85 1071 3035 Fragments arranged by size: 3035 1126 1071 85 BsmI GAATG_Cn' Cuts at: 2828 2921 2828 Size: 93 5224 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2102 2252 2636 3378 3463 4534 Size: 399 264 48 150 384 742 85 1071 Cuts at: 4534 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 384 264 150 85 48 BspEI T'CCGG_A Cuts at: 4980 4980 Size: 5317 BspHI T'CATG_A Cuts at: 3206 4936 3206 Size: 1730 3587 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2206 2672 844 Size: 1362 466 3489 Fragments arranged by size: 3489 1362 466 BsrBI CCG'CTC Cuts at: 1394 1823 3204 1394 Size: 429 1381 3507 Fragments arranged by size: 3507 1381 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3812 3986 65 Size: 1183 2564 174 1396 Fragments arranged by size: 2564 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3961 4148 1700 Size: 110 2151 187 2869 Fragments arranged by size: 2869 2151 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5317 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5317 BssSI C'ACGA_G Cuts at: 3377 4389 3377 Size: 1012 4305 BstAPI GCAn_nnn'nTGC Cuts at: 794 2272 794 Size: 1478 3839 BstBI TT'CG_AA Cuts at: 4121 4121 Size: 5317 BstEII G'GTnAC_C Cuts at: 1184 2548 1184 Size: 1364 3953 BstYI r'GATC_y Cuts at: 1972 2647 3014 3399 3416 4868 4954 4966 5312 Size: 675 367 385 17 1452 86 12 346 Cuts at: 5312 1972 Size: 1977 Fragments arranged by size: 1977 1452 675 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3826 Fragments arranged by size: 3826 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4578 Fragments arranged by size: 4578 683 56 BtsI GCAGTG_nn' Cuts at: 2833 3627 3647 2833 Size: 794 20 4503 Fragments arranged by size: 4503 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5317 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2159 2194 529 Size: 35 1595 35 3652 Fragments arranged by size: 3652 1595 35 35 DraI TTT'AAA Cuts at: 2621 2621 Size: 5317 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4324 809 Size: 507 3008 1802 Fragments arranged by size: 3008 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2764 3653 Size: 54 1425 50 275 18 234 700 889 Cuts at: 3653 5282 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 700 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2764 1537 Size: 275 952 4090 Fragments arranged by size: 4090 952 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2126 2325 2367 3247 1353 Size: 773 199 42 880 3423 Fragments arranged by size: 3423 880 773 199 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 3904 4278 4424 387 Size: 1064 138 504 1811 374 146 1280 Fragments arranged by size: 1811 1280 1064 504 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2427 3380 3977 4764 5032 1239 Size: 393 795 953 597 787 268 1524 Fragments arranged by size: 1524 953 795 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2050 2218 2050 Size: 168 5149 EcoRI G'AATT_C Cuts at: 2626 3004 2626 Size: 378 4939 FspI TGC'GCA Cuts at: 3823 3823 Size: 5317 HaeII r_GCGC'y Cuts at: 1678 2037 2273 2611 4464 1678 Size: 359 236 338 1853 2531 Fragments arranged by size: 2531 1853 359 338 236 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3958 3990 4032 4064 1373 Size: 32 135 32 2386 32 42 32 2626 Fragments arranged by size: 2626 2386 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2661 2907 669 Size: 988 281 723 246 3079 Fragments arranged by size: 3079 988 723 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5317 HpaI GTT'AAC Cuts at: 2907 2907 Size: 5317 KasI G'GCGC_C Cuts at: 2033 2607 2033 Size: 574 4743 KpnI G_GTAC'C Cuts at: 2642 2642 Size: 5317 MfeI C'AATT_G Cuts at: 2916 2916 Size: 5317 MluI A'CGCG_T Cuts at: 5193 5193 Size: 5317 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4431 4615 1305 Size: 3126 184 2007 Fragments arranged by size: 3126 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3495 Fragments arranged by size: 3495 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3275 3634 3793 518 Size: 648 2109 359 159 2042 Fragments arranged by size: 2109 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2733 3410 4558 4803 1606 Size: 1127 677 1148 245 2120 Fragments arranged by size: 2120 1148 1127 677 245 NarI GG'CG_CC Cuts at: 2034 2608 2034 Size: 574 4743 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3826 Fragments arranged by size: 3826 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5317 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5317 NotI GC'GGCC_GC Cuts at: 2764 2764 Size: 5317 NspI r_CATG'y Cuts at: 1132 1229 2266 2675 4220 1132 Size: 97 1037 409 1545 2229 Fragments arranged by size: 2229 1545 1037 409 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5317 PciI A'CATG_T Cuts at: 4216 4216 Size: 5317 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5317 PmeI GTTT'AAAC Cuts at: 2621 2621 Size: 5317 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3386 3418 4899 4931 3386 Size: 32 1481 32 3772 Fragments arranged by size: 3772 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2218 2218 Size: 5317 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5317 PsiI TTA'TAA Cuts at: 2887 2887 Size: 5317 PspOMI G'GGCC_C Cuts at: 2050 2050 Size: 5317 PstI C_TGCA'G Cuts at: 830 2669 830 Size: 1839 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5317 SalI G'TCGA_C Cuts at: 1936 2659 1936 Size: 723 4594 SbfI CC_TGCA'GG Cuts at: 2669 2669 Size: 5317 ScaI AGT'ACT Cuts at: 3565 5277 3565 Size: 1712 3605 SfcI C'TryA_G Cuts at: 826 1045 2665 3800 4481 4672 826 Size: 219 1620 1135 681 191 1471 Fragments arranged by size: 1620 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2035 2609 2035 Size: 574 4743 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5317 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5317 SmaI CCC'GGG Cuts at: 1872 2055 2644 1872 Size: 183 589 4545 Fragments arranged by size: 4545 589 183 SmlI C'TyrA_G Cuts at: 820 1017 1943 3421 4322 4584 820 Size: 197 926 1478 901 262 1553 Fragments arranged by size: 1553 1478 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3108 493 Size: 2615 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5317 SphI G_CATG'C Cuts at: 1132 2675 1132 Size: 1543 3774 SspI AAT'ATT Cuts at: 5 52 3241 5 Size: 47 3189 2081 Fragments arranged by size: 3189 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5317 StyI C'CwwG_G Cuts at: 513 1065 2004 2226 2756 513 Size: 552 939 222 530 3074 Fragments arranged by size: 3074 939 552 530 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3344 3361 3514 3699 4144 325 Size: 1255 1764 17 153 185 445 1498 Fragments arranged by size: 1764 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3563 5275 96 Size: 274 80 33 51 1443 1586 1712 138 Fragments arranged by size: 1712 1586 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5277 5309 5277 Size: 32 5285 Tth111I GACn'n_nGTC Cuts at: 1316 2558 1316 Size: 1242 4075 XbaI T'CTAG_A Cuts at: 2653 2653 Size: 5317 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5317 XmaI C'CCGG_G Cuts at: 1870 2053 2642 1870 Size: 183 589 4545 Fragments arranged by size: 4545 589 183 XmnI GAAnn'nnTTC Cuts at: 3446 3446 Size: 5317 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3125 276 Size: 53 83 186 936 1591 2468 Fragments arranged by size: 2468 1591 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI