Restriction map for
pFN21AB8175
(Circular) (Six-base) MAPSORT of: pFN21AB8175.seq Check: 4701 from: 1 to: 6208
pFN21AB8175 6208 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2067 4018 278
Size: 53 83 186 936 531 1951 2468
Fragments arranged by size:
2468 1951 936 531 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 2480 2567 3390 4271 5655 5923 1632
Size: 734 114 87 823 881 1384 268 1917
Fragments arranged by size:
1917 1384 881 823 734 268 114 87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 5107 6084 1203
Size: 3904 977 1327
Fragments arranged by size:
3904 1327 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6208
AlwNI CAG_nnn'CTG
Cuts at: 3951 5523 3951
Size: 1572 4636
ApaI G_GGCC'C
Cuts at: 3261 3261
Size: 6208
ApaLI G'TGCA_C
Cuts at: 4265 5421 4265
Size: 1156 5052
ApoI r'AATT_y
Cuts at: 1493 2252 2282 2330 3517 3743 3895 6004 1493
Size: 759 30 48 1187 226 152 2109 1697
Fragments arranged by size:
2109 1697 1187 759 226 152 48 30
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 2352 3005 3533 1870
Size: 73 190 219 653 528 4545
Fragments arranged by size:
4545 653 528 219 190 73
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2930 3529 4984 618
Size: 325 217 627 1143 599 1455 1842
Fragments arranged by size:
1842 1455 1143 627 599 325 217
BanII G_rGCy'C
Cuts at: 1391 2220 2731 2851 3261 3527 1391
Size: 829 511 120 410 266 4072
Fragments arranged by size:
4072 829 511 410 266 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3450 3479 928
Size: 971 1551 29 3657
Fragments arranged by size:
3657 1551 971 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4399 4433 1903
Size: 34 2462 34 3678
Fragments arranged by size:
3678 2462 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4100 5310 4100
Size: 1210 4998
BclI T'GATC_A
Cuts at: 1548 2122 1548
Size: 574 5634
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4819 136
Size: 107 122 71 631 605 3147 1525
Fragments arranged by size:
3147 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2307 2982 6203 2307
Size: 675 3221 2312
Fragments arranged by size:
3221 2312 675
BlpI GC'TnA_GC
Cuts at: 3625 3625
Size: 6208
Bme1580I G_kGCm'C
Cuts at: 1790 2713 2933 3261 4269 5425 1790
Size: 923 220 328 1008 1156 2573
Fragments arranged by size:
2573 1156 1008 923 328 220
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3939 4897 442
Size: 416 53 83 399 24 2522 958 1753
Fragments arranged by size:
2522 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2745 2903 4868 1239
Size: 800 706 158 1965 2579
Fragments arranged by size:
2579 1965 800 706 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2252 2429 2594 3433 4333 5198 5496 2252
Size: 177 165 839 900 865 298 2964
Fragments arranged by size:
2964 900 865 839 298 177 165
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4871 882
Size: 617 3372 2219
Fragments arranged by size:
3372 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3999 493
Size: 711 2795 2702
Fragments arranged by size:
2795 2702 711
BsaBI GATnn'nnATC
Cuts at: 2794 6202 2794
Size: 3408 2800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 3292 4015 4397
Size: 53 83 186 936 531 1228 723 382
Cuts at: 4397 5032 275
Size: 635 1451
Fragments arranged by size:
1451 1228 936 723 635 531 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2289 4641 5039 5313 5460 5871 2289
Size: 2352 398 274 147 411 2626
Fragments arranged by size:
2626 2352 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5983
Fragments arranged by size:
5983 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2228 2743 2777 1186
Size: 871 171 515 34 4617
Fragments arranged by size:
4617 871 515 171 34
BseYI C'CCAG_C
Cuts at: 3162 5411 5907 3162
Size: 2249 496 3463
Fragments arranged by size:
3463 2249 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5635
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3658 4419 5447 1540
Size: 275 186 1657 761 1028 2301
Fragments arranged by size:
2301 1657 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 4269 4354 5425 2220
Size: 2049 85 1071 3003
Fragments arranged by size:
3003 2049 1071 85
BsmI GAATG_Cn'
Cuts at: 2545 2747 3719 3812 2545
Size: 202 972 93 4941
Fragments arranged by size:
4941 972 202 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2713 2731 2851 2933 3261 3527
Size: 399 430 493 18 120 82 328 266
Cuts at: 3527 4269 4354 5425 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 493 430 399 328 266
120 85 82 18
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 2038 3028 4097 5827 2038
Size: 990 1069 1730 2419
Fragments arranged by size:
2419 1730 1069 990
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3159 3563 844
Size: 2315 404 3489
Fragments arranged by size:
3489 2315 404
BsrBI CCG'CTC
Cuts at: 1394 1823 4095 1394
Size: 429 2272 3507
Fragments arranged by size:
3507 2272 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2961 4703 4877 65
Size: 1183 1713 1742 174 1396
Fragments arranged by size:
1742 1713 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4852 5039 1700
Size: 110 3042 187 2869
Fragments arranged by size:
3042 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3037 96
Size: 2941 3267
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 4268 5280 4268
Size: 1012 5196
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6208
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstXI CCAn_nnnn'nTGG
Cuts at: 3162 3162
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 2307 2982 3484 3538 3905 4290 4307 5759
Size: 335 675 502 54 367 385 17 1452
Cuts at: 5759 5845 5857 6203 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 675 502 385 367 346 335
86 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2174 2174
Size: 6208
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4717
Fragments arranged by size:
4717 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5469
Fragments arranged by size:
5469 683 56
BtsI GCAGTG_nn'
Cuts at: 2713 3330 3724 4518 4538 2713
Size: 617 394 794 20 4383
Fragments arranged by size:
4383 794 617 394 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2307 2342 529
Size: 35 1743 35 4395
Fragments arranged by size:
4395 1743 35 35
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5215 809
Size: 507 3899 1802
Fragments arranged by size:
3899 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2690 3655 4544
Size: 54 1425 50 275 18 860 965 889
Cuts at: 4544 6173 8
Size: 1629 43
Fragments arranged by size:
1629 1425 965 889 860 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3655 1537
Size: 275 1843 4090
Fragments arranged by size:
4090 1843 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2364 2397 2835 3060 3072 3081 3114 4138
Size: 1011 33 438 225 12 9 33 1024
Cuts at: 4138 1353
Size: 3423
Fragments arranged by size:
3423 1024 1011 438 225 33 33 12
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4795 5169 5315 387
Size: 1064 138 3206 374 146 1280
Fragments arranged by size:
3206 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2218 2218
Size: 6208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2366 2480 2567 2745 2903 3390
Size: 393 407 327 114 87 178 158 487
Cuts at: 3390 4271 4868 5655 5923 1239
Size: 881 597 787 268 1524
Fragments arranged by size:
1524 881 787 597 487 407 393 327
268 178 158 114 87
EcoO109I rG'GnC_Cy
Cuts at: 2234 2676 2832 3257 2234
Size: 442 156 425 5185
Fragments arranged by size:
5185 442 425 156
EcoRI G'AATT_C
Cuts at: 2252 2282 3517 3895 2252
Size: 30 1235 378 4565
Fragments arranged by size:
4565 1235 378 30
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 2035 5355 1678
Size: 357 3320 2531
Fragments arranged by size:
3320 2531 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3356 3388 4849 4881 4923
Size: 32 135 32 1784 32 1461 32 42
Cuts at: 4923 4955 1373
Size: 32 2626
Fragments arranged by size:
2626 1784 1461 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3552 3798 669
Size: 988 281 1614 246 3079
Fragments arranged by size:
3079 1614 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3197 748
Size: 2449 3759
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5322 5506 1305
Size: 4017 184 2007
Fragments arranged by size:
4017 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4386
Fragments arranged by size:
4386 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2301 3033 3300 4166 4525 4684 518
Size: 648 1135 732 267 866 359 159 2042
Fragments arranged by size:
2042 1135 866 732 648 359 267 159
MspA1I CmG'CkG
Cuts at: 1606 2029 3624 4301 5449 5694 1606
Size: 423 1595 677 1148 245 2120
Fragments arranged by size:
2120 1595 1148 677 423 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4717
Fragments arranged by size:
4717 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 3566 5111 1132
Size: 97 2337 1545 2229
Fragments arranged by size:
2337 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6208
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6208
PfoI T'CCnGG_A
Cuts at: 2103 2683 2103
Size: 580 5628
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2234 2676 2832 2234
Size: 442 156 5610
Fragments arranged by size:
5610 442 156
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PspOMI G'GGCC_C
Cuts at: 3257 3257
Size: 6208
PstI C_TGCA'G
Cuts at: 830 3560 830
Size: 2730 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
SacI G_AGCT'C
Cuts at: 2220 2220
Size: 6208
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SanDI GG'GwC_CC
Cuts at: 2832 2832
Size: 6208
SapI GCTCTTCn'nnn_
Cuts at: 2835 3060 3081 2835
Size: 225 21 5962
Fragments arranged by size:
5962 225 21
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SfcI C'TryA_G
Cuts at: 826 1045 3556 4691 5372 5563 826
Size: 219 2511 1135 681 191 1471
Fragments arranged by size:
2511 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 3535 1872
Size: 1663 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 2352 2444 2573 3448 4312
Size: 197 926 324 85 92 129 875 864
Cuts at: 4312 5213 5475 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 875 864 324 262 197
129 92 85
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6208
StyI C'CwwG_G
Cuts at: 513 1065 2004 3647 513
Size: 552 939 1643 3074
Fragments arranged by size:
3074 1643 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2313 4235 4252 4405 4590 5035 325
Size: 1255 733 1922 17 153 185 445 1498
Fragments arranged by size:
1922 1498 1255 733 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 3037 4454
Size: 274 80 33 51 1443 471 589 1417
Cuts at: 4454 6166 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1417 589 471 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6168 6200 6168
Size: 32 6176
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 2971 3544 2971
Size: 573 5635
XhoI C'TCGA_G
Cuts at: 1943 2352 1943
Size: 409 5799
XmaI C'CCGG_G
Cuts at: 1870 3533 1870
Size: 1663 4545
XmnI GAAnn'nnTTC
Cuts at: 4337 4337
Size: 6208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2065 4016 276
Size: 53 83 186 936 531 1951 2468
Fragments arranged by size:
2468 1951 936 531 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacII SexAI SfiI SfoI SrfI SwaI XcmI