Restriction map for
pFN21AB8174
(Circular) (Six-base) MAPSORT of: pFN21AB8174.seq Check: 5201 from: 1 to: 6394
pFN21AB8174 6394 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4204 278
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3737 1937
Size: 1800 4594
Acc65I G'GTAC_C
Cuts at: 2050 3715 2050
Size: 1665 4729
AclI AA'CG_TT
Cuts at: 1563 4521 4894 1563
Size: 2958 373 3063
Fragments arranged by size:
3063 2958 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 2103 2420 3591 4457 5841 6109 1632
Size: 447 24 317 1171 866 1384 268 1917
Fragments arranged by size:
1917 1384 1171 866 447 317 268 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6197
AflIII A'CryG_T
Cuts at: 1203 5293 6270 1203
Size: 4090 977 1327
Fragments arranged by size:
4090 1327 977
AgeI A'CCGG_T
Cuts at: 5225 5225
Size: 6394
AhdI GACnn_n'nnGTC
Cuts at: 5123 5123
Size: 6394
AleI CACnn'nnGTG
Cuts at: 1166 3209 1166
Size: 2043 4351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6394
AlwNI CAG_nnn'CTG
Cuts at: 2876 3569 4137 5709 2876
Size: 693 568 1572 3561
Fragments arranged by size:
3561 1572 693 568
ApaLI G'TGCA_C
Cuts at: 4451 5607 4451
Size: 1156 5238
ApoI r'AATT_y
Cuts at: 1493 2519 2727 3703 3929 4081 6190 1493
Size: 1026 208 976 226 152 2109 1697
Fragments arranged by size:
2109 1697 1026 976 226 208 152
AseI AT'TA_AT
Cuts at: 160 1032 4948 160
Size: 872 3916 1606
Fragments arranged by size:
3916 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
AvaI C'yCGr_G
Cuts at: 1870 1943 3719 1870
Size: 73 1776 4545
Fragments arranged by size:
4545 1776 73
BamHI G'GATC_C
Cuts at: 3724 4091 3724
Size: 367 6027
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 3715 5170 618
Size: 325 217 627 263 1665 1455 1842
Fragments arranged by size:
1842 1665 1455 627 325 263 217
BanII G_rGCy'C
Cuts at: 1391 2551 3152 3266 3401 3713 1391
Size: 1160 601 114 135 312 4072
Fragments arranged by size:
4072 1160 601 312 135 114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 928
Size: 971 192 5231
Fragments arranged by size:
5231 971 192
BbvCI CC'TCA_GC
Cuts at: 3254 3303 3611 3254
Size: 49 308 6037
Fragments arranged by size:
6037 308 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4585 4619 1903
Size: 34 2648 34 3678
Fragments arranged by size:
3678 2648 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4286 5496 4286
Size: 1210 5184
BclI T'GATC_A
Cuts at: 1548 2347 2355 2532 1548
Size: 799 8 177 5410
Fragments arranged by size:
5410 799 177 8
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5005 136
Size: 107 122 71 631 605 3333 1525
Fragments arranged by size:
3333 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6389 6389
Size: 6394
BlpI GC'TnA_GC
Cuts at: 3395 3811 3395
Size: 416 5978
Bme1580I G_kGCm'C
Cuts at: 1790 4455 5611 1790
Size: 2665 1156 2573
Fragments arranged by size:
2665 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6394
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2024 3203 3437
Size: 416 53 83 399 24 607 1179 234
Cuts at: 3437 4125 5083 442
Size: 688 958 1753
Fragments arranged by size:
1753 1179 958 688 607 416 399 234
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3171 3171
Size: 6394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2535 3104 3250 5054 1239
Size: 1296 569 146 1804 2579
Fragments arranged by size:
2579 1804 1296 569 146
Bpu10I CC'TnA_GC
Cuts at: 2441 3096 3254 3303 3611 2441
Size: 655 158 49 308 5224
Fragments arranged by size:
5224 655 308 158 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3166 4519 5384 5682 3166
Size: 1353 865 298 3878
Fragments arranged by size:
3878 1353 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3334 5057 882
Size: 617 1835 1723 2219
Fragments arranged by size:
2219 1835 1723 617
BsaAI yAC'GTr
Cuts at: 493 1204 4185 493
Size: 711 2981 2702
Fragments arranged by size:
2981 2702 711
BsaBI GATnn'nnATC
Cuts at: 2621 6388 2621
Size: 3767 2627
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4201 4583 5218 275
Size: 53 83 186 936 2668 382 635 1451
Fragments arranged by size:
2668 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2459 4827 5225 5499 5646 6057 2459
Size: 2368 398 274 147 411 2796
Fragments arranged by size:
2796 2368 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6169
Fragments arranged by size:
6169 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2714 2831 3029 3147 3322 3635 1186
Size: 1096 432 117 198 118 175 313 3945
Fragments arranged by size:
3945 1096 432 313 198 175 118 117
BseYI C'CCAG_C
Cuts at: 2144 5597 6093 2144
Size: 3453 496 2445
Fragments arranged by size:
3453 2445 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2600 1163
Size: 573 864 4957
Fragments arranged by size:
4957 864 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3844 4605 5633 1540
Size: 275 186 1843 761 1028 2301
Fragments arranged by size:
2301 1843 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2551 3092 3401 4455 4540 5611 2551
Size: 541 309 1054 85 1071 3334
Fragments arranged by size:
3334 1071 1054 541 309 85
BsmI GAATG_Cn'
Cuts at: 3905 3998 3905
Size: 93 6301
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2551 3092 3152 3266 3401 3713 4455
Size: 399 761 541 60 114 135 312 742
Cuts at: 4455 4540 5611 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 761 742 541 399 312 135
114 85 60
BspEI T'CCGG_A
Cuts at: 2459 6057 2459
Size: 3598 2796
BspHI T'CATG_A
Cuts at: 4283 6013 4283
Size: 1730 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2513 3749 844
Size: 1669 1236 3489
Fragments arranged by size:
3489 1669 1236
BsrBI CCG'CTC
Cuts at: 1394 1823 4281 1394
Size: 429 2458 3507
Fragments arranged by size:
3507 2458 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4889 5063 65
Size: 1183 3641 174 1396
Fragments arranged by size:
3641 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5038 5225 1700
Size: 110 3228 187 2869
Fragments arranged by size:
3228 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6394
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6394
BssSI C'ACGA_G
Cuts at: 4454 5466 4454
Size: 1012 5382
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6394
BstBI TT'CG_AA
Cuts at: 5198 5198
Size: 6394
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6394
BstYI r'GATC_y
Cuts at: 1972 3724 4091 4476 4493 5945 6031 6043 6389
Size: 1752 367 385 17 1452 86 12 346
Cuts at: 6389 1972
Size: 1977
Fragments arranged by size:
1977 1752 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2688 2694 3228 3444 3471 513
Size: 552 939 684 6 534 216 27 3436
Fragments arranged by size:
3436 939 684 552 534 216 27 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5655
Fragments arranged by size:
5655 683 56
BtsI GCAGTG_nn'
Cuts at: 2545 3287 3910 4704 4724 2545
Size: 742 623 794 20 4215
Fragments arranged by size:
4215 794 742 623 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6394
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6359
DraI TTT'AAA
Cuts at: 3698 3698
Size: 6394
DraIII CAC_nnn'GTG
Cuts at: 3670 3670
Size: 6394
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5401 809
Size: 507 4085 1802
Fragments arranged by size:
4085 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2691 3125 3246
Size: 54 1425 50 275 18 861 434 121
Cuts at: 3246 3468 3841 4730 6359 8
Size: 222 373 889 1629 43
Fragments arranged by size:
1629 1425 889 861 434 373 275 222
121 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3841 1537
Size: 275 2029 4090
Fragments arranged by size:
4090 2029 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2405 2625 2637 2778 3314 4324 1353
Size: 1052 220 12 141 536 1010 3423
Fragments arranged by size:
3423 1052 1010 536 220 141 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4981 5355 5501 387
Size: 1064 138 3392 374 146 1280
Fragments arranged by size:
3392 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2549 3399 2549
Size: 850 5544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2103 2420 2535 3104 3250 3591
Size: 393 447 24 317 115 569 146 341
Cuts at: 3591 4457 5054 5841 6109 1239
Size: 866 597 787 268 1524
Fragments arranged by size:
1524 866 787 597 569 447 393 341
317 268 146 115 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2058 3257 2058
Size: 1199 5195
EcoO109I rG'GnC_Cy
Cuts at: 3111 3111
Size: 6394
EcoRI G'AATT_C
Cuts at: 3703 4081 3703
Size: 378 6016
FspI TGC'GCA
Cuts at: 4900 4900
Size: 6394
HaeII r_GCGC'y
Cuts at: 1678 3392 5541 1678
Size: 1714 2149 2531
Fragments arranged by size:
2531 2149 1714
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5035 5067 5109 5141 1373
Size: 32 135 32 3463 32 42 32 2626
Fragments arranged by size:
3463 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3738 3984 669
Size: 988 281 1800 246 3079
Fragments arranged by size:
3079 1800 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6394
HpaI GTT'AAC
Cuts at: 3984 3984
Size: 6394
KpnI G_GTAC'C
Cuts at: 2054 3719 2054
Size: 1665 4729
MfeI C'AATT_G
Cuts at: 3993 3993
Size: 6394
MluI A'CGCG_T
Cuts at: 6270 6270
Size: 6394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 2991 5508 5692 1305
Size: 825 861 2517 184 2007
Fragments arranged by size:
2517 2007 861 825 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2693 3248 3470 10
Size: 54 1425 343 861 555 222 2934
Fragments arranged by size:
2934 1425 861 555 343 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 3209 4352 4711 4870 518
Size: 648 1578 465 1143 359 159 2042
Fragments arranged by size:
2042 1578 1143 648 465 359 159
MspA1I CmG'CkG
Cuts at: 1606 2678 2842 3124 3810 4487 5635 5880 1606
Size: 1072 164 282 686 677 1148 245 2120
Fragments arranged by size:
2120 1148 1072 686 677 282 245 164
NcoI C'CATG_G
Cuts at: 513 1065 2004 2688 3228 3444 3471 513
Size: 552 939 684 540 216 27 3436
Fragments arranged by size:
3436 939 684 552 540 216 27
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6394
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6394
NotI GC'GGCC_GC
Cuts at: 3841 3841
Size: 6394
NspI r_CATG'y
Cuts at: 1132 1229 3752 5297 1132
Size: 97 2523 1545 2229
Fragments arranged by size:
2523 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6394
PciI A'CATG_T
Cuts at: 5293 5293
Size: 6394
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6394
PfoI T'CCnGG_A
Cuts at: 2314 2314
Size: 6394
PmeI GTTT'AAAC
Cuts at: 3698 3698
Size: 6394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2964 2996 4463 4495 5976 6008 2964
Size: 32 1467 32 1481 32 3350
Fragments arranged by size:
3350 1481 1467 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6394
PsiI TTA'TAA
Cuts at: 3964 3964
Size: 6394
PstI C_TGCA'G
Cuts at: 830 2510 3746 830
Size: 1680 1236 3478
Fragments arranged by size:
3478 1680 1236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6394
SacI G_AGCT'C
Cuts at: 2551 3401 2551
Size: 850 5544
SalI G'TCGA_C
Cuts at: 1936 3736 1936
Size: 1800 4594
SapI GCTCTTCn'nnn_
Cuts at: 3314 3314
Size: 6394
SbfI CC_TGCA'GG
Cuts at: 3746 3746
Size: 6394
ScaI AGT'ACT
Cuts at: 4642 6354 4642
Size: 1712 4682
SfcI C'TryA_G
Cuts at: 826 1045 2226 2506 3742 4877 5558 5749 826
Size: 219 1181 280 1236 1135 681 191 1471
Fragments arranged by size:
1471 1236 1181 1135 681 280 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6394
SmaI CCC'GGG
Cuts at: 1872 3721 1872
Size: 1849 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3145 4498 5399 5661 820
Size: 197 926 1202 1353 901 262 1553
Fragments arranged by size:
1553 1353 1202 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4185 493
Size: 3692 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6394
SphI G_CATG'C
Cuts at: 1132 3752 1132
Size: 2620 3774
SspI AAT'ATT
Cuts at: 5 52 3238 4318 5
Size: 47 3186 1080 2081
Fragments arranged by size:
3186 2081 1080 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6394
StyI C'CwwG_G
Cuts at: 513 1065 2004 2688 3228 3444 3471 3833 513
Size: 552 939 684 540 216 27 362 3074
Fragments arranged by size:
3074 939 684 552 540 362 216 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2417 2602 3208 4421 4438 4591 4776
Size: 1255 837 185 606 1213 17 153 185
Cuts at: 4776 5221 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1213 837 606 445 185 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3422 4640 6352
Size: 274 80 33 51 1443 1445 1218 1712
Cuts at: 6352 96
Size: 138
Fragments arranged by size:
1712 1445 1443 1218 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2310 2342 6354 6386 2310
Size: 32 4012 32 2318
Fragments arranged by size:
4012 2318 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2921 1316
Size: 1605 4789
XbaI T'CTAG_A
Cuts at: 3730 3730
Size: 6394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3208 3208
Size: 6394
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6394
XmaI C'CCGG_G
Cuts at: 1870 3719 1870
Size: 1849 4545
XmnI GAAnn'nnTTC
Cuts at: 2976 4523 2976
Size: 1547 4847
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4202 276
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI