Restriction map for
pFN21AB8171
(Circular) (Six-base) MAPSORT of: pFN21AB8171.seq Check: 4899 from: 1 to: 6376
pFN21AB8171 6376 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2219 2219
Size: 6376
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3719 1937
Size: 1782 4594
Acc65I G'GTAC_C
Cuts at: 3697 3697
Size: 6376
AclI AA'CG_TT
Cuts at: 1563 4503 4876 1563
Size: 2940 373 3063
Fragments arranged by size:
3063 2940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 2420 2466 2503 3418 3511 3600 4439
Size: 707 81 46 37 915 93 89 839
Cuts at: 4439 5823 6091 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 915 839 707 268 93 89
81 46 37
AflII C'TTAA_G
Cuts at: 820 1017 2611 820
Size: 197 1594 4585
Fragments arranged by size:
4585 1594 197
AflIII A'CryG_T
Cuts at: 1203 5275 6252 1203
Size: 4072 977 1327
Fragments arranged by size:
4072 1327 977
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6376
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2962 2963 2994 2995 3480 3512 2962
Size: 1 31 1 485 32 5826
Fragments arranged by size:
5826 485 32 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2067 2275 2578 2849 3451 4119 5691 2067
Size: 208 303 271 602 668 1572 2752
Fragments arranged by size:
2752 1572 668 602 303 271 208
ApaLI G'TGCA_C
Cuts at: 2350 2582 4433 5589 2350
Size: 232 1851 1156 3137
Fragments arranged by size:
3137 1851 1156 232
ApoI r'AATT_y
Cuts at: 1493 2544 2647 2735 3174 3685 3911 4063 6172
Size: 1051 103 88 439 511 226 152 2109
Cuts at: 6172 1493
Size: 1697
Fragments arranged by size:
2109 1697 1051 511 439 226 152 103
88
AseI AT'TA_AT
Cuts at: 160 1032 4930 160
Size: 872 3898 1606
Fragments arranged by size:
3898 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 3363 3701 1870
Size: 73 1420 338 4545
Fragments arranged by size:
4545 1420 338 73
BamHI G'GATC_C
Cuts at: 3706 4073 3706
Size: 367 6009
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3095 3697 5152 618
Size: 325 217 627 1308 602 1455 1842
Fragments arranged by size:
1842 1455 1308 627 602 325 217
BanII G_rGCy'C
Cuts at: 1391 3695 1391
Size: 2304 4072
BbeI G_GCGC'C
Cuts at: 3099 3099
Size: 6376
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3159 3200 928
Size: 971 1260 41 4104
Fragments arranged by size:
4104 1260 971 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4567 4601 1903
Size: 34 2630 34 3678
Fragments arranged by size:
3678 2630 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3096 4268 5478 3096
Size: 1172 1210 3994
Fragments arranged by size:
3994 1210 1172
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6376
BfrBI ATG'CAT
Cuts at: 2884 2884
Size: 6376
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4987 136
Size: 107 122 71 631 605 3315 1525
Fragments arranged by size:
3315 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6371 6371
Size: 6376
BlpI GC'TnA_GC
Cuts at: 3793 3793
Size: 6376
Bme1580I G_kGCm'C
Cuts at: 1790 2354 2586 3525 4437 5593 1790
Size: 564 232 939 912 1156 2573
Fragments arranged by size:
2573 1156 939 912 564 232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6376
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2057 2216 2710
Size: 416 53 83 399 24 640 159 494
Cuts at: 2710 4107 5065 442
Size: 1397 958 1753
Fragments arranged by size:
1753 1397 958 640 494 416 399 159
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3093 3534 5036 1239
Size: 1854 441 1502 2579
Fragments arranged by size:
2579 1854 1502 441
Bpu10I CC'TnA_GC
Cuts at: 3213 3213
Size: 6376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 4501 5366 5664 2138
Size: 2363 865 298 2850
Fragments arranged by size:
2850 2363 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2658 3127 5039 882
Size: 617 1159 469 1912 2219
Fragments arranged by size:
2219 1912 1159 617 469
BsaAI yAC'GTr
Cuts at: 493 1204 4167 493
Size: 711 2963 2702
Fragments arranged by size:
2963 2702 711
BsaBI GATnn'nnATC
Cuts at: 6370 6370
Size: 6376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3096 4183 4565 5200
Size: 53 83 186 936 1563 1087 382 635
Cuts at: 5200 275
Size: 1451
Fragments arranged by size:
1563 1451 1087 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4809 5207 5481 5628 6039 4809
Size: 398 274 147 411 5146
Fragments arranged by size:
5146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2690 2720 3480 3510 1178
Size: 30 165 30 1287 30 760 30 4044
Fragments arranged by size:
4044 1287 760 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2778 1186
Size: 1090 502 4784
Fragments arranged by size:
4784 1090 502
BseYI C'CCAG_C
Cuts at: 2617 5579 6075 2617
Size: 2962 496 2918
Fragments arranged by size:
2962 2918 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2288 2807 3189 1163
Size: 573 552 519 382 4350
Fragments arranged by size:
4350 573 552 519 382
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3826 4587 5615 1540
Size: 275 186 1825 761 1028 2301
Fragments arranged by size:
2301 1825 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2354 2586 4437 4522 5593 2354
Size: 232 1851 85 1071 3137
Fragments arranged by size:
3137 1851 1071 232 85
BsmI GAATG_Cn'
Cuts at: 2483 2884 3887 3980 2483
Size: 401 1003 93 4879
Fragments arranged by size:
4879 1003 401 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2354 2586 3525 3695 4437 4522 5593
Size: 399 564 232 939 170 742 85 1071
Cuts at: 5593 1391
Size: 2174
Fragments arranged by size:
2174 1071 939 742 564 399 232 170
85
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 3127 4265 5995 3127
Size: 1138 1730 3508
Fragments arranged by size:
3508 1730 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2219 3731 844
Size: 1375 1512 3489
Fragments arranged by size:
3489 1512 1375
BsrBI CCG'CTC
Cuts at: 1394 1823 4263 1394
Size: 429 2440 3507
Fragments arranged by size:
3507 2440 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2467 2775 3240 4871 5045 65
Size: 1183 1219 308 465 1631 174 1396
Fragments arranged by size:
1631 1396 1219 1183 465 308 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2676 5020 5207 1700
Size: 110 866 2344 187 2869
Fragments arranged by size:
2869 2344 866 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6376
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 4436 5448 4436
Size: 1012 5364
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2275 2349 2489 2581 794
Size: 1481 74 140 92 4589
Fragments arranged by size:
4589 1481 140 92 74
BstBI TT'CG_AA
Cuts at: 5180 5180
Size: 6376
BstEII G'GTnAC_C
Cuts at: 1184 3477 1184
Size: 2293 4083
BstXI CCAn_nnnn'nTGG
Cuts at: 2047 2047
Size: 6376
BstYI r'GATC_y
Cuts at: 1972 3706 4073 4458 4475 5927 6013 6025 6371
Size: 1734 367 385 17 1452 86 12 346
Cuts at: 6371 1972
Size: 1977
Fragments arranged by size:
1977 1734 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5824
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3288 487
Size: 683 56 2062 3575
Fragments arranged by size:
3575 2062 683 56
BtsI GCAGTG_nn'
Cuts at: 2180 2795 2946 3892 4686 4706 2180
Size: 615 151 946 794 20 3850
Fragments arranged by size:
3850 946 794 615 151 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3223 3258 529
Size: 35 2659 35 3647
Fragments arranged by size:
3647 2659 35 35
DraI TTT'AAA
Cuts at: 3680 3680
Size: 6376
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2144 5383 809
Size: 507 828 3239 1802
Fragments arranged by size:
3239 1802 828 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2359 3354 3823
Size: 54 1425 50 275 18 529 995 469
Cuts at: 3823 4712 6341 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 995 889 529 469 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3823 1537
Size: 275 2011 4090
Fragments arranged by size:
4090 2011 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4306 1353
Size: 2953 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4963 5337 5483 387
Size: 1064 138 3374 374 146 1280
Fragments arranged by size:
3374 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2420 2466 2503 3093 3418 3511
Size: 393 707 81 46 37 590 325 93
Cuts at: 3511 3534 3600 4439 5036 5823 6091 1239
Size: 23 66 839 597 787 268 1524
Fragments arranged by size:
1524 839 787 707 597 590 393 325
268 93 81 66 46 37 23
EcoO109I rG'GnC_Cy
Cuts at: 2962 2962
Size: 6376
EcoRI G'AATT_C
Cuts at: 2735 3174 3685 4063 2735
Size: 439 511 378 5048
Fragments arranged by size:
5048 511 439 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 2740 2533
Size: 207 6169
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 2275 3099 5523 1678
Size: 597 824 2424 2531
Fragments arranged by size:
2531 2424 824 597
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2149 2181 2690 2722 2845
Size: 32 135 32 577 32 509 32 123
Cuts at: 2845 2877 3495 3527 5017 5049 5091 5123 1373
Size: 32 618 32 1490 32 42 32 2626
Fragments arranged by size:
2626 1490 618 577 509 135 123 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2188 3720 3966 669
Size: 988 281 250 1532 246 3079
Fragments arranged by size:
3079 1532 988 281 250 246
HindIII A'AGCT_T
Cuts at: 748 3024 748
Size: 2276 4100
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KasI G'GCGC_C
Cuts at: 3095 3095
Size: 6376
KpnI G_GTAC'C
Cuts at: 3701 3701
Size: 6376
MfeI C'AATT_G
Cuts at: 3975 3975
Size: 6376
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3244 5490 5674 1305
Size: 1939 2246 184 2007
Fragments arranged by size:
2246 2007 1939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2361 10
Size: 54 1425 343 529 4025
Fragments arranged by size:
4025 1425 529 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 3350 3410 3446 4334 4693 4852
Size: 648 1269 915 60 36 888 359 159
Cuts at: 4852 518
Size: 2042
Fragments arranged by size:
2042 1269 915 888 648 359 159 60
36
MspA1I CmG'CkG
Cuts at: 1606 2036 2578 2661 2976 3554 3792 4469 5617
Size: 430 542 83 315 578 238 677 1148
Cuts at: 5617 5862 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 578 542 430 315 245
238 83
NaeI GCC'GGC
Cuts at: 2678 2678
Size: 6376
NarI GG'CG_CC
Cuts at: 3096 3096
Size: 6376
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5824
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6376
NgoMIV G'CCGG_C
Cuts at: 2676 2676
Size: 6376
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6376
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NsiI A_TGCA'T
Cuts at: 2886 2886
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 3445 3734 5279 1132
Size: 97 2216 289 1545 2229
Fragments arranged by size:
2229 2216 1545 289 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6376
PciI A'CATG_T
Cuts at: 5275 5275
Size: 6376
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3598 1108
Size: 2490 3886
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3480 3512 4445 4477 5958 5990 3480
Size: 32 933 32 1481 32 3866
Fragments arranged by size:
3866 1481 933 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2962 2962
Size: 6376
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PstI C_TGCA'G
Cuts at: 830 3728 830
Size: 2898 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
PvuII CAG'CTG
Cuts at: 2578 2976 3554 2578
Size: 398 578 5400
Fragments arranged by size:
5400 578 398
SalI G'TCGA_C
Cuts at: 1936 3718 1936
Size: 1782 4594
SanDI GG'GwC_CC
Cuts at: 2962 2962
Size: 6376
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SfcI C'TryA_G
Cuts at: 826 1045 2937 3724 4859 5540 5731 826
Size: 219 1892 787 1135 681 191 1471
Fragments arranged by size:
1892 1471 1135 787 681 219 191
SfoI GGC'GCC
Cuts at: 3097 3097
Size: 6376
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 3365 3703 1872
Size: 1493 338 4545
Fragments arranged by size:
4545 1493 338
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 2611 4480 5381 5643 820
Size: 197 926 210 458 1869 901 262 1553
Fragments arranged by size:
1869 1553 926 901 458 262 210 197
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 3734 1132
Size: 2602 3774
SspI AAT'ATT
Cuts at: 5 52 4300 5
Size: 47 4248 2081
Fragments arranged by size:
4248 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6376
StyI C'CwwG_G
Cuts at: 513 1065 2335 3294 3483 3815 513
Size: 552 1270 959 189 332 3074
Fragments arranged by size:
3074 1270 959 552 332 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2897 3496 4403 4420 4573 4758 5203
Size: 1255 1317 599 907 17 153 185 445
Cuts at: 5203 325
Size: 1498
Fragments arranged by size:
1498 1317 1255 907 599 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2811 3114
Size: 274 80 33 51 1443 249 585 303
Cuts at: 3114 4622 6334 96
Size: 1508 1712 138
Fragments arranged by size:
1712 1508 1443 585 303 274 249 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6336 6368 6336
Size: 32 6344
Tth111I GACn'n_nGTC
Cuts at: 1316 3509 1316
Size: 2193 4183
XbaI T'CTAG_A
Cuts at: 3712 3712
Size: 6376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 3363 3701 1870
Size: 1493 338 4545
Fragments arranged by size:
4545 1493 338
XmnI GAAnn'nnTTC
Cuts at: 2547 2630 4505 2547
Size: 83 1875 4418
Fragments arranged by size:
4418 1875 83
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NruI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SrfI SwaI
XcmI