Restriction map for
pFN21AB8170
(Circular) (Six-base) MAPSORT of: pFN21AB8170.seq Check: 4754 from: 1 to: 5812
pFN21AB8170 5812 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3155 1937
Size: 1218 4594
Acc65I G'GTAC_C
Cuts at: 3133 3133
Size: 5812
AclI AA'CG_TT
Cuts at: 1563 3939 4312 1563
Size: 2376 373 3063
Fragments arranged by size:
3063 2376 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2276 2366 2457 2555 2747 2905 3083
Size: 452 192 90 91 98 192 158 178
Cuts at: 3083 3875 5259 5527 1632
Size: 792 1384 268 1917
Fragments arranged by size:
1917 1384 792 452 268 192 192 178
158 98 91 90
AfeI AGC'GCT
Cuts at: 2600 2600
Size: 5812
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5615
AflIII A'CryG_T
Cuts at: 1203 2856 4711 5688 1203
Size: 1653 1855 977 1327
Fragments arranged by size:
1855 1653 1327 977
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 2047 4541 2047
Size: 2494 3318
AleI CACnn'nnGTG
Cuts at: 1166 2087 1166
Size: 921 4891
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5812
AlwNI CAG_nnn'CTG
Cuts at: 2320 2466 2654 2806 2879 3555 5127 2320
Size: 146 188 152 73 676 1572 3005
Fragments arranged by size:
3005 1572 676 188 152 146 73
ApaI G_GGCC'C
Cuts at: 2351 2608 2644 2351
Size: 257 36 5519
Fragments arranged by size:
5519 257 36
ApaLI G'TGCA_C
Cuts at: 3869 5025 3869
Size: 1156 4656
ApoI r'AATT_y
Cuts at: 1493 2405 3121 3347 3499 5608 1493
Size: 912 716 226 152 2109 1697
Fragments arranged by size:
2109 1697 912 716 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4366 160
Size: 872 3334 1606
Fragments arranged by size:
3334 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 2643 3137 1870
Size: 73 700 494 4545
Fragments arranged by size:
4545 700 494 73
BamHI G'GATC_C
Cuts at: 2436 3142 3509 2436
Size: 706 367 4739
Fragments arranged by size:
4739 706 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2181 2777 3133 4588 618
Size: 325 217 627 394 596 356 1455 1842
Fragments arranged by size:
1842 1455 627 596 394 356 325 217
BanII G_rGCy'C
Cuts at: 1391 2287 2351 2484 2608 2644 3131 1391
Size: 896 64 133 124 36 487 4072
Fragments arranged by size:
4072 896 487 133 124 64 36
BbeI G_GCGC'C
Cuts at: 2185 2185
Size: 5812
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2160 928
Size: 971 261 4580
Fragments arranged by size:
4580 971 261
BbvCI CC'TCA_GC
Cuts at: 3107 3107
Size: 5812
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2104 2138 4003 4037 1903
Size: 34 167 34 1865 34 3678
Fragments arranged by size:
3678 1865 167 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4423 136
Size: 107 122 71 631 605 2751 1525
Fragments arranged by size:
2751 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5807 5807
Size: 5812
BlpI GC'TnA_GC
Cuts at: 2556 3036 3057 3229 2556
Size: 480 21 172 5139
Fragments arranged by size:
5139 480 172 21
Bme1580I G_kGCm'C
Cuts at: 1790 2351 2608 2644 2782 3079 3873 5029 1790
Size: 561 257 36 138 297 794 1156 2573
Fragments arranged by size:
2573 1156 794 561 297 257 138 36
BmgBI CAC'GTC
Cuts at: 1318 2428 1318
Size: 1110 4702
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3543 4501 442
Size: 416 53 83 399 24 2126 958 1753
Fragments arranged by size:
2126 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2174 2642 2966 4472 1239
Size: 929 6 468 324 1506 2579
Fragments arranged by size:
2579 1506 929 468 324 6
Bpu10I CC'TnA_GC
Cuts at: 3107 3107
Size: 5812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2216 2543 3937 4802 5100 2216
Size: 327 1394 865 298 2928
Fragments arranged by size:
2928 1394 865 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2150 2262 4475 882
Size: 617 651 112 2213 2219
Fragments arranged by size:
2219 2213 651 617 112
BsaAI yAC'GTr
Cuts at: 493 1204 3603 493
Size: 711 2399 2702
Fragments arranged by size:
2702 2399 711
BsaBI GATnn'nnATC
Cuts at: 5806 5806
Size: 5812
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2182 3619 4001 4636
Size: 53 83 186 936 649 1437 382 635
Cuts at: 4636 275
Size: 1451
Fragments arranged by size:
1451 1437 936 649 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4245 4643 4917 5064 5475 4245
Size: 398 274 147 411 4582
Fragments arranged by size:
4582 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5587
Fragments arranged by size:
5587 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2522 2540 2639 2978 1186
Size: 955 381 18 99 339 4020
Fragments arranged by size:
4020 955 381 339 99 18
BseYI C'CCAG_C
Cuts at: 2260 2595 2601 2742 5015 5511 2260
Size: 335 6 141 2273 496 2561
Fragments arranged by size:
2561 2273 496 335 141 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5239
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3262 4023 5051 1540
Size: 275 186 1261 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2287 3873 3958 5029 2287
Size: 1586 85 1071 3070
Fragments arranged by size:
3070 1586 1071 85
BsmI GAATG_Cn'
Cuts at: 3323 3416 3323
Size: 93 5719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2287 2351 2484 2608 2644 2782 3079
Size: 399 497 64 133 124 36 138 297
Cuts at: 3079 3131 3873 3958 5029 1391
Size: 52 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 497 399 297 138 133
124 85 64 52 36
BspEI T'CCGG_A
Cuts at: 5475 5475
Size: 5812
BspHI T'CATG_A
Cuts at: 3701 5431 3701
Size: 1730 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2625 3111 3167 844
Size: 1781 486 56 3489
Fragments arranged by size:
3489 1781 486 56
BsrBI CCG'CTC
Cuts at: 1394 1823 3699 1394
Size: 429 1876 3507
Fragments arranged by size:
3507 1876 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4307 4481 65
Size: 1183 3059 174 1396
Fragments arranged by size:
3059 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2178 4456 4643 1700
Size: 110 368 2278 187 2869
Fragments arranged by size:
2869 2278 368 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5812
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5812
BssSI C'ACGA_G
Cuts at: 3872 4884 3872
Size: 1012 4800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3074 3103 794
Size: 2280 29 3503
Fragments arranged by size:
3503 2280 29
BstBI TT'CG_AA
Cuts at: 4616 4616
Size: 5812
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5812
BstXI CCAn_nnnn'nTGG
Cuts at: 2279 2279
Size: 5812
BstYI r'GATC_y
Cuts at: 1972 2211 2391 2436 2568 3142 3509 3894 3911
Size: 239 180 45 132 574 367 385 17
Cuts at: 3911 5363 5449 5461 5807 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 574 385 367 346 239 180
132 86 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2432 513
Size: 552 1367 3893
Fragments arranged by size:
3893 1367 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5073
Fragments arranged by size:
5073 683 56
BtsI GCAGTG_nn'
Cuts at: 2094 3328 4122 4142 2094
Size: 1234 794 20 3764
Fragments arranged by size:
3764 1234 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5777
DraI TTT'AAA
Cuts at: 3116 3116
Size: 5812
DraIII CAC_nnn'GTG
Cuts at: 3074 3074
Size: 5812
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2164 4819 809
Size: 507 848 2655 1802
Fragments arranged by size:
2655 1802 848 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3259 4148 5777
Size: 54 1425 50 275 18 1429 889 1629
Cuts at: 5777 8
Size: 43
Fragments arranged by size:
1629 1429 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3259 1537
Size: 275 1447 4090
Fragments arranged by size:
4090 1447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3742 1353
Size: 2389 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4399 4773 4919 387
Size: 1064 138 2810 374 146 1280
Fragments arranged by size:
2810 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2285 2285
Size: 5812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2168 2174 2276 2366 2457 2555
Size: 393 452 84 6 102 90 91 98
Cuts at: 2555 2642 2747 2905 2966 3083 3875 4472 5259
Size: 87 105 158 61 117 792 597 787
Cuts at: 5259 5527 1239
Size: 268 1524
Fragments arranged by size:
1524 792 787 597 452 393 268 158
117 105 102 98 91 90 87 84
61 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2355 2621 2798 2810 2355
Size: 266 177 12 5357
Fragments arranged by size:
5357 266 177 12
EcoO109I rG'GnC_Cy
Cuts at: 2348 2614 2640 2803 2980 3028 2348
Size: 266 26 163 177 48 5132
Fragments arranged by size:
5132 266 177 163 48 26
EcoRI G'AATT_C
Cuts at: 2405 3121 3499 2405
Size: 716 378 4718
Fragments arranged by size:
4718 716 378
FspI TGC'GCA
Cuts at: 4318 4318
Size: 5812
HaeII r_GCGC'y
Cuts at: 1678 2185 2602 2794 4959 1678
Size: 507 417 192 2165 2531
Fragments arranged by size:
2531 2165 507 417 192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 4453 4485 4527
Size: 32 135 32 461 32 2388 32 42
Cuts at: 4527 4559 1373
Size: 32 2626
Fragments arranged by size:
2626 2388 461 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3156 3402 669
Size: 988 281 1218 246 3079
Fragments arranged by size:
3079 1218 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5812
HpaI GTT'AAC
Cuts at: 3402 3402
Size: 5812
KasI G'GCGC_C
Cuts at: 2181 2181
Size: 5812
KpnI G_GTAC'C
Cuts at: 3137 3137
Size: 5812
MfeI C'AATT_G
Cuts at: 3411 3411
Size: 5812
MluI A'CGCG_T
Cuts at: 5688 5688
Size: 5812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4926 5110 1305
Size: 3621 184 2007
Fragments arranged by size:
3621 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3990
Fragments arranged by size:
3990 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 3770 4129 4288 518
Size: 648 921 1683 359 159 2042
Fragments arranged by size:
2042 1683 921 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2651 3228 3905 5053 5298 1606
Size: 540 505 577 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 577 540 505 245
NaeI GCC'GGC
Cuts at: 2180 2180
Size: 5812
NarI GG'CG_CC
Cuts at: 2182 2182
Size: 5812
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5260
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5812
NgoMIV G'CCGG_C
Cuts at: 2178 2178
Size: 5812
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5812
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 2860 3170 4715 1132
Size: 97 1631 310 1545 2229
Fragments arranged by size:
2229 1631 1545 310 97
PasI CC'CwG_GG
Cuts at: 1348 2578 2977 1348
Size: 1230 399 4183
Fragments arranged by size:
4183 1230 399
PciI A'CATG_T
Cuts at: 2856 4711 2856
Size: 1855 3957
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5812
PfoI T'CCnGG_A
Cuts at: 2394 2394
Size: 5812
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 5394 5426 3881
Size: 32 1481 32 4267
Fragments arranged by size:
4267 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2614 3028 2614
Size: 414 5398
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 3382 3382
Size: 5812
PspOMI G'GGCC_C
Cuts at: 2347 2604 2640 2347
Size: 257 36 5519
Fragments arranged by size:
5519 257 36
PstI C_TGCA'G
Cuts at: 830 2145 2802 3164 830
Size: 1315 657 362 3478
Fragments arranged by size:
3478 1315 657 362
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
PvuII CAG'CTG
Cuts at: 2146 2146
Size: 5812
SacI G_AGCT'C
Cuts at: 2287 2287
Size: 5812
SalI G'TCGA_C
Cuts at: 1936 3154 1936
Size: 1218 4594
SbfI CC_TGCA'GG
Cuts at: 2802 3164 2802
Size: 362 5450
ScaI AGT'ACT
Cuts at: 4060 5772 4060
Size: 1712 4100
SfcI C'TryA_G
Cuts at: 826 1045 2141 2798 3160 4295 4976 5167 826
Size: 219 1096 657 362 1135 681 191 1471
Fragments arranged by size:
1471 1135 1096 681 657 362 219 191
SfoI GGC'GCC
Cuts at: 2183 2183
Size: 5812
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 2645 3139 1872
Size: 773 494 4545
Fragments arranged by size:
4545 773 494
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2195 2522 3916 4817 5079 820
Size: 197 926 252 327 1394 901 262 1553
Fragments arranged by size:
1553 1394 926 901 327 262 252 197
SnaBI TAC'GTA
Cuts at: 493 3603 493
Size: 3110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 3170 1132
Size: 2038 3774
SrfI GCCC'GGGC
Cuts at: 2645 2645
Size: 5812
SspI AAT'ATT
Cuts at: 5 52 2821 3736 5
Size: 47 2769 915 2081
Fragments arranged by size:
2769 2081 915 47
StuI AGG'CCT
Cuts at: 1517 2676 1517
Size: 1159 4653
StyI C'CwwG_G
Cuts at: 513 1065 3251 513
Size: 552 2186 3074
Fragments arranged by size:
3074 2186 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2708 3839 3856 4009 4194 4639 325
Size: 1255 1128 1131 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1131 1128 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4058 5770 96
Size: 274 80 33 51 1443 2081 1712 138
Fragments arranged by size:
2081 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5772 5804 5772
Size: 32 5780
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5812
XbaI T'CTAG_A
Cuts at: 3148 3148
Size: 5812
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5812
XmaI C'CCGG_G
Cuts at: 1870 2643 3137 1870
Size: 773 494 4545
Fragments arranged by size:
4545 773 494
XmnI GAAnn'nnTTC
Cuts at: 3941 3941
Size: 5812
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SwaI XcmI