Restriction map for
pFN21AB8168
(Circular) (Six-base) MAPSORT of: pFN21AB8168.seq Check: 2273 from: 1 to: 5617
pFN21AB8168 5617 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 1937
Size: 1023 4594
Acc65I G'GTAC_C
Cuts at: 2230 2938 2230
Size: 708 4909
AclI AA'CG_TT
Cuts at: 1563 3744 4117 1563
Size: 2181 373 3063
Fragments arranged by size:
3063 2181 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2832 3680 5064 5332 1632
Size: 1200 848 1384 268 1917
Fragments arranged by size:
1917 1384 1200 848 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 4516 5493 1203
Size: 3313 977 1327
Fragments arranged by size:
3313 1327 977
AgeI A'CCGG_T
Cuts at: 2719 4448 2719
Size: 1729 3888
AhdI GACnn_n'nnGTC
Cuts at: 4346 4346
Size: 5617
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2274 2373 1184
Size: 1090 99 4428
Fragments arranged by size:
4428 1090 99
AlwNI CAG_nnn'CTG
Cuts at: 3360 4932 3360
Size: 1572 4045
ApaLI G'TGCA_C
Cuts at: 3674 4830 3674
Size: 1156 4461
ApoI r'AATT_y
Cuts at: 1493 2926 3152 3304 5413 1493
Size: 1433 226 152 2109 1697
Fragments arranged by size:
2109 1697 1433 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2942 1870
Size: 73 78 921 4545
Fragments arranged by size:
4545 921 78 73
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2230 2513 2618 2680 2938
Size: 325 217 627 443 283 105 62 258
Cuts at: 2938 4393 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 443 325 283 258 217
105 62
BanII G_rGCy'C
Cuts at: 1391 2017 2666 2846 2936 1391
Size: 626 649 180 90 4072
Fragments arranged by size:
4072 649 626 180 90
BbeI G_GCGC'C
Cuts at: 2517 2684 2517
Size: 167 5450
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2545 2785 928
Size: 971 646 240 3760
Fragments arranged by size:
3760 971 646 240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3678 1871 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3509 4719 3509
Size: 1210 4407
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4228 136
Size: 107 122 71 631 605 2556 1525
Fragments arranged by size:
2556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5612 5612
Size: 5617
BlpI GC'TnA_GC
Cuts at: 2262 3034 2262
Size: 772 4845
Bme1580I G_kGCm'C
Cuts at: 1790 2621 3678 4834 1790
Size: 831 1057 1156 2573
Fragments arranged by size:
2573 1156 1057 831
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2229 3348 4306
Size: 416 53 83 399 24 812 1119 958
Cuts at: 4306 442
Size: 1753
Fragments arranged by size:
1753 1119 958 812 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2124 2047
Size: 77 5540
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4277 1239
Size: 3038 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2119 3742 4607 4905 2119
Size: 1623 865 298 2831
Fragments arranged by size:
2831 1623 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2048 2184 4280 882
Size: 617 549 136 2096 2219
Fragments arranged by size:
2219 2096 617 549 136
BsaAI yAC'GTr
Cuts at: 493 1204 3408 493
Size: 711 2204 2702
Fragments arranged by size:
2702 2204 711
BsaBI GATnn'nnATC
Cuts at: 5611 5611
Size: 5617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2514 2681 3424 3806
Size: 53 83 186 936 981 167 743 382
Cuts at: 3806 4441 275
Size: 635 1451
Fragments arranged by size:
1451 981 936 743 635 382 186 167
83 53
BsaWI w'CCGG_w
Cuts at: 2193 2674 2719 4050 4448 4722 4869 5280 2193
Size: 481 45 1331 398 274 147 411 2530
Fragments arranged by size:
2530 1331 481 411 398 274 147 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2709 2739 2901 2931 1178
Size: 30 165 30 1306 30 162 30 3864
Fragments arranged by size:
3864 1306 165 162 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2761 1186
Size: 837 738 4042
Fragments arranged by size:
4042 837 738
BseYI C'CCAG_C
Cuts at: 2493 2659 4820 5316 2493
Size: 166 2161 496 2794
Fragments arranged by size:
2794 2161 496 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5044
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3067 3828 4856 1540
Size: 275 186 1066 761 1028 2301
Fragments arranged by size:
2301 1066 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2017 2727 3678 3763 4834 2017
Size: 710 951 85 1071 2800
Fragments arranged by size:
2800 1071 951 710 85
BsmI GAATG_Cn'
Cuts at: 2396 3128 3221 2396
Size: 732 93 4792
Fragments arranged by size:
4792 732 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2621 2666 2727 2846 2936 3678
Size: 399 227 604 45 61 119 90 742
Cuts at: 3678 3763 4834 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 604 399 227 119 90
85 61 45
BspEI T'CCGG_A
Cuts at: 2674 5280 2674
Size: 2606 3011
BspHI T'CATG_A
Cuts at: 3506 5236 3506
Size: 1730 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2972 844
Size: 2128 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2511 3504 1394
Size: 429 688 993 3507
Fragments arranged by size:
3507 993 688 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4112 4286 65
Size: 1183 2864 174 1396
Fragments arranged by size:
2864 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2719 4261 4448 1700
Size: 110 909 1542 187 2869
Fragments arranged by size:
2869 1542 909 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5617
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2405 794
Size: 1611 4006
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 2734 1184
Size: 1550 4067
BstXI CCAn_nnnn'nTGG
Cuts at: 2065 2065
Size: 5617
BstYI r'GATC_y
Cuts at: 1972 2255 2280 2947 3314 3699 3716 5168 5254
Size: 283 25 667 367 385 17 1452 86
Cuts at: 5254 5266 5612 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 667 385 367 346 283 86
25 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2295 2605 2622 2634 513
Size: 552 1230 310 17 12 3496
Fragments arranged by size:
3496 1230 552 310 17 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2557 487
Size: 683 56 1331 3547
Fragments arranged by size:
3547 1331 683 56
BtsI GCAGTG_nn'
Cuts at: 3133 3927 3947 3133
Size: 794 20 4803
Fragments arranged by size:
4803 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2921 2921
Size: 5617
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4624 809
Size: 507 3308 1802
Fragments arranged by size:
3308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2602 3064
Size: 54 1425 50 275 18 318 454 462
Cuts at: 3064 3953 5582 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 462 454 318 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3064 1537
Size: 275 1252 4090
Fragments arranged by size:
4090 1252 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3547 1353
Size: 2194 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2731 4204 4578 4724 387
Size: 1064 138 1142 1473 374 146 1280
Fragments arranged by size:
1473 1280 1142 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2015 2015
Size: 5617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2832 3680 4277 5064 5332 1239
Size: 393 1200 848 597 787 268 1524
Fragments arranged by size:
1524 1200 848 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2926 3304 2926
Size: 378 5239
FspI TGC'GCA
Cuts at: 4123 4123
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 2185 2517 2684 2699 4764 1678
Size: 507 332 167 15 2065 2531
Fragments arranged by size:
2531 2065 507 332 167 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2181 2213 2284 2316 2709
Size: 32 135 32 609 32 71 32 393
Cuts at: 2709 2741 4258 4290 4332 4364 1373
Size: 32 1517 32 42 32 2626
Fragments arranged by size:
2626 1517 609 393 135 71 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2961 3207 669
Size: 988 281 1023 246 3079
Fragments arranged by size:
3079 1023 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 5617
KasI G'GCGC_C
Cuts at: 2513 2680 2513
Size: 167 5450
KpnI G_GTAC'C
Cuts at: 2234 2942 2234
Size: 708 4909
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4731 4915 1305
Size: 3426 184 2007
Fragments arranged by size:
3426 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 10
Size: 54 1425 343 318 3477
Fragments arranged by size:
3477 1425 343 318 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 3575 3934 4093 518
Size: 648 897 1512 359 159 2042
Fragments arranged by size:
2042 1512 897 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2497 2584 3033 3710 4858 5103 1606
Size: 891 87 449 677 1148 245 2120
Fragments arranged by size:
2120 1148 891 677 449 245 87
NarI GG'CG_CC
Cuts at: 2514 2681 2514
Size: 167 5450
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5065
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5617
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2975 4520 1132
Size: 97 1746 1545 2229
Fragments arranged by size:
2229 1746 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2541 1348
Size: 1193 4424
PciI A'CATG_T
Cuts at: 4516 4516
Size: 5617
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2146 1108
Size: 1038 4579
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 5199 5231 3686
Size: 32 1481 32 4072
Fragments arranged by size:
4072 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2289 2969 830
Size: 1459 680 3478
Fragments arranged by size:
3478 1459 680
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
RsrII CG'GwC_CG
Cuts at: 2717 2717
Size: 5617
SacI G_AGCT'C
Cuts at: 2017 2017
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2285 2884 2965 4100 4781 4972 826
Size: 219 1240 599 81 1135 681 191 1471
Fragments arranged by size:
1471 1240 1135 681 599 219 191 81
SfoI GGC'GCC
Cuts at: 2515 2682 2515
Size: 167 5450
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2944 1872
Size: 1072 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2098 3721 4622 4884 820
Size: 197 926 155 1623 901 262 1553
Fragments arranged by size:
1623 1553 926 901 262 197 155
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2975 1132
Size: 1843 3774
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5617
StyI C'CwwG_G
Cuts at: 513 1065 2872 2891 3056 513
Size: 552 1807 19 165 3074
Fragments arranged by size:
3074 1807 552 165 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2647 2779 2869 3644 3661 3814 3999
Size: 1255 1067 132 90 775 17 153 185
Cuts at: 3999 4444 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1067 775 445 185 153 132
90 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2025 3863 5575
Size: 274 80 33 51 1443 48 1838 1712
Cuts at: 5575 96
Size: 138
Fragments arranged by size:
1838 1712 1443 274 138 80 51 48
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5577 5609 5577
Size: 32 5585
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5617
XbaI T'CTAG_A
Cuts at: 2953 2953
Size: 5617
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5617
XmaI C'CCGG_G
Cuts at: 1870 2942 1870
Size: 1072 4545
XmnI GAAnn'nnTTC
Cuts at: 3746 3746
Size: 5617
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI RsrII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI