Restriction map for
pFN21AB8160
(Circular) (Six-base) MAPSORT of: pFN21AB8160.seq Check: 5914 from: 1 to: 5485
pFN21AB8160 5485 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3295 278
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 1937
Size: 891 4594
Acc65I G'GTAC_C
Cuts at: 2806 2806
Size: 5485
AclI AA'CG_TT
Cuts at: 1563 3612 3985 1563
Size: 2049 373 3063
Fragments arranged by size:
3063 2049 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3548 4932 5200 1632
Size: 1916 1384 268 1917
Fragments arranged by size:
1917 1916 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5288
AflIII A'CryG_T
Cuts at: 1203 2310 4384 5361 1203
Size: 1107 2074 977 1327
Fragments arranged by size:
2074 1327 1107 977
AgeI A'CCGG_T
Cuts at: 4316 4316
Size: 5485
AhdI GACnn_n'nnGTC
Cuts at: 4214 4214
Size: 5485
AleI CACnn'nnGTG
Cuts at: 1166 2417 1166
Size: 1251 4234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5485
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2174 2206 2174
Size: 32 5453
AlwNI CAG_nnn'CTG
Cuts at: 2154 2428 3228 4800 2154
Size: 274 800 1572 2839
Fragments arranged by size:
2839 1572 800 274
ApaLI G'TGCA_C
Cuts at: 3542 4698 3542
Size: 1156 4329
ApoI r'AATT_y
Cuts at: 1493 2085 2188 2777 2794 3020 3172 5281 1493
Size: 592 103 589 17 226 152 2109 1697
Fragments arranged by size:
2109 1697 592 589 226 152 103 17
AseI AT'TA_AT
Cuts at: 160 1032 4039 160
Size: 872 3007 1606
Fragments arranged by size:
3007 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
AvaI C'yCGr_G
Cuts at: 1870 1943 2810 1870
Size: 73 867 4545
Fragments arranged by size:
4545 867 73
BamHI G'GATC_C
Cuts at: 2815 3182 2815
Size: 367 5118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2273 2653 2806 4261 618
Size: 325 217 627 486 380 153 1455 1842
Fragments arranged by size:
1842 1455 627 486 380 325 217 153
BanII G_rGCy'C
Cuts at: 1391 2052 2148 2804 1391
Size: 661 96 656 4072
Fragments arranged by size:
4072 661 656 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2313 928
Size: 971 414 4100
Fragments arranged by size:
4100 971 414
BbvCI CC'TCA_GC
Cuts at: 2714 2714
Size: 5485
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3676 3710 1903
Size: 34 1739 34 3678
Fragments arranged by size:
3678 1739 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4587 3377
Size: 1210 4275
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5485
BfrBI ATG'CAT
Cuts at: 2649 2649
Size: 5485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2255 4096 136
Size: 107 122 71 631 605 583 1841 1525
Fragments arranged by size:
1841 1525 631 605 583 122 107 71
BglII A'GATC_T
Cuts at: 5480 5480
Size: 5485
BlpI GC'TnA_GC
Cuts at: 2147 2902 2147
Size: 755 4730
Bme1580I G_kGCm'C
Cuts at: 1790 2478 2670 2766 3546 4702 1790
Size: 688 192 96 780 1156 2573
Fragments arranged by size:
2573 1156 780 688 192 96
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5485
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2755 3216 4174
Size: 416 53 83 399 24 1338 461 958
Cuts at: 4174 442
Size: 1753
Fragments arranged by size:
1753 1338 958 461 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2002 2066 2505 4145 1239
Size: 763 64 439 1640 2579
Fragments arranged by size:
2579 1640 763 439 64
Bpu10I CC'TnA_GC
Cuts at: 2714 2714
Size: 5485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3610 4475 4773 3610
Size: 865 298 4322
Fragments arranged by size:
4322 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4148 882
Size: 617 2649 2219
Fragments arranged by size:
2649 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3276 493
Size: 711 2072 2702
Fragments arranged by size:
2702 2072 711
BsaBI GATnn'nnATC
Cuts at: 5479 5479
Size: 5485
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3292 3674 4309 275
Size: 53 83 186 936 1759 382 635 1451
Fragments arranged by size:
1759 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3918 4316 4590 4737 5148 3918
Size: 398 274 147 411 4255
Fragments arranged by size:
4255 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5260
Fragments arranged by size:
5260 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2337 1186
Size: 1151 4334
BseYI C'CCAG_C
Cuts at: 4688 5184 4688
Size: 496 4989
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 2441 1163
Size: 573 579 126 4207
Fragments arranged by size:
4207 579 573 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2935 3696 4724 1540
Size: 275 186 934 761 1028 2301
Fragments arranged by size:
2301 1028 934 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2052 2148 3546 3631 4702 2052
Size: 96 1398 85 1071 2835
Fragments arranged by size:
2835 1398 1071 96 85
BsmI GAATG_Cn'
Cuts at: 2300 2651 2996 3089 2300
Size: 351 345 93 4696
Fragments arranged by size:
4696 351 345 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2148 2478 2670 2766 2804 3546
Size: 399 262 96 330 192 96 38 742
Cuts at: 3546 3631 4702 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 330 262 192 96
96 85 38
BspEI T'CCGG_A
Cuts at: 5148 5148
Size: 5485
BspHI T'CATG_A
Cuts at: 2302 3374 5104 2302
Size: 1072 1730 2683
Fragments arranged by size:
2683 1730 1072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2614 2840 844
Size: 1770 226 3489
Fragments arranged by size:
3489 1770 226
BsrBI CCG'CTC
Cuts at: 1394 1823 3372 1394
Size: 429 1549 3507
Fragments arranged by size:
3507 1549 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2466 2478 3980 4154 65
Size: 1183 1218 12 1502 174 1396
Fragments arranged by size:
1502 1396 1218 1183 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2656 4129 4316 1700
Size: 110 846 1473 187 2869
Fragments arranged by size:
2869 1473 846 187 110
BsrGI T'GTAC_A
Cuts at: 96 2754 96
Size: 2658 2827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5485
BssSI C'ACGA_G
Cuts at: 2669 3545 4557 2669
Size: 876 1012 3597
Fragments arranged by size:
3597 1012 876
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2572 794
Size: 1778 3707
BstBI TT'CG_AA
Cuts at: 2533 4289 2533
Size: 1756 3729
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5485
BstYI r'GATC_y
Cuts at: 1972 2727 2815 3182 3567 3584 5036 5122 5134
Size: 755 88 367 385 17 1452 86 12
Cuts at: 5134 5480 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 755 385 367 346 88 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2365 2522 2365
Size: 157 5328
BtgI C'CryG_G
Cuts at: 513 1065 2004 2058 2203 513
Size: 552 939 54 145 3795
Fragments arranged by size:
3795 939 552 145 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4746
Fragments arranged by size:
4746 683 56
BtsI GCAGTG_nn'
Cuts at: 3001 3795 3815 3001
Size: 794 20 4671
Fragments arranged by size:
4671 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5450
DraI TTT'AAA
Cuts at: 2789 2789
Size: 5485
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4492 809
Size: 507 3176 1802
Fragments arranged by size:
3176 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2932 3821
Size: 54 1425 50 275 18 231 871 889
Cuts at: 3821 5450 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 871 275 231 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2932 1537
Size: 275 1120 4090
Fragments arranged by size:
4090 1120 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3415 1353
Size: 2062 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4072 4446 4592 387
Size: 1064 138 2483 374 146 1280
Fragments arranged by size:
2483 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2050 2146 2050
Size: 96 5389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2066 2505 3548 4145 4932 5200
Size: 393 370 64 439 1043 597 787 268
Cuts at: 5200 1239
Size: 1524
Fragments arranged by size:
1524 1043 787 597 439 393 370 268
64
EcoRI G'AATT_C
Cuts at: 2777 2794 3172 2777
Size: 17 378 5090
Fragments arranged by size:
5090 378 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 5485
FspI TGC'GCA
Cuts at: 3991 3991
Size: 5485
HaeII r_GCGC'y
Cuts at: 1678 2154 4632 1678
Size: 476 2478 2531
Fragments arranged by size:
2531 2478 476
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2327 2359 4126
Size: 32 135 32 428 32 295 32 1767
Cuts at: 4126 4158 4200 4232 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1767 428 295 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2829 3075 669
Size: 988 281 891 246 3079
Fragments arranged by size:
3079 988 891 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5485
HpaI GTT'AAC
Cuts at: 3075 3075
Size: 5485
KpnI G_GTAC'C
Cuts at: 2810 2810
Size: 5485
MfeI C'AATT_G
Cuts at: 3084 3084
Size: 5485
MluI A'CGCG_T
Cuts at: 5361 5361
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4599 4783 1305
Size: 3294 184 2007
Fragments arranged by size:
3294 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3663
Fragments arranged by size:
3663 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 2293 2417 2471 3443 3802 3961
Size: 648 897 230 124 54 972 359 159
Cuts at: 3961 518
Size: 2042
Fragments arranged by size:
2042 972 897 648 359 230 159 124
54
MspA1I CmG'CkG
Cuts at: 1606 2044 2462 2563 2901 3578 4726 4971 1606
Size: 438 418 101 338 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 438 418 338 245 101
NcoI C'CATG_G
Cuts at: 513 1065 2004 2058 2203 513
Size: 552 939 54 145 3795
Fragments arranged by size:
3795 939 552 145 54
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5485
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5485
NotI GC'GGCC_GC
Cuts at: 2932 2932
Size: 5485
NsiI A_TGCA'T
Cuts at: 2651 2651
Size: 5485
NspI r_CATG'y
Cuts at: 1132 1229 2314 2755 2843 4388 1132
Size: 97 1085 441 88 1545 2229
Fragments arranged by size:
2229 1545 1085 441 97 88
PasI CC'CwG_GG
Cuts at: 1348 2352 1348
Size: 1004 4481
PciI A'CATG_T
Cuts at: 2310 4384 2310
Size: 2074 3411
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5485
PmeI GTTT'AAAC
Cuts at: 2789 2789
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2435 2467 3554 3586 5067 5099 2435
Size: 32 1087 32 1481 32 2821
Fragments arranged by size:
2821 1481 1087 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5485
PsiI TTA'TAA
Cuts at: 3055 3055
Size: 5485
PstI C_TGCA'G
Cuts at: 830 2043 2556 2837 830
Size: 1213 513 281 3478
Fragments arranged by size:
3478 1213 513 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5485
PvuII CAG'CTG
Cuts at: 2044 2044
Size: 5485
SacI G_AGCT'C
Cuts at: 2052 2148 2052
Size: 96 5389
SalI G'TCGA_C
Cuts at: 1936 2827 1936
Size: 891 4594
SbfI CC_TGCA'GG
Cuts at: 2837 2837
Size: 5485
ScaI AGT'ACT
Cuts at: 3733 5445 3733
Size: 1712 3773
SfcI C'TryA_G
Cuts at: 826 1045 2039 2552 2590 2833 3968 4649 4840
Size: 219 994 513 38 243 1135 681 191
Cuts at: 4840 826
Size: 1471
Fragments arranged by size:
1471 1135 994 681 513 243 219 191
38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5485
SmaI CCC'GGG
Cuts at: 1872 2812 1872
Size: 940 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3589 4490 4752 820
Size: 197 926 1646 901 262 1553
Fragments arranged by size:
1646 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3276 493
Size: 2783 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5485
SphI G_CATG'C
Cuts at: 1132 2843 1132
Size: 1711 3774
SspI AAT'ATT
Cuts at: 5 52 3409 5
Size: 47 3357 2081
Fragments arranged by size:
3357 2081 47
StuI AGG'CCT
Cuts at: 1517 2363 1517
Size: 846 4639
StyI C'CwwG_G
Cuts at: 513 1065 2004 2058 2203 2415 2924 513
Size: 552 939 54 145 212 509 3074
Fragments arranged by size:
3074 939 552 509 212 145 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2216 3512 3529 3682 3867 4312 325
Size: 1255 636 1296 17 153 185 445 1498
Fragments arranged by size:
1498 1296 1255 636 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2754 3731 5443
Size: 274 80 33 51 1443 777 977 1712
Cuts at: 5443 96
Size: 138
Fragments arranged by size:
1712 1443 977 777 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5445 5477 5445
Size: 32 5453
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5485
XbaI T'CTAG_A
Cuts at: 2162 2821 2162
Size: 659 4826
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2287 2287
Size: 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5485
XmaI C'CCGG_G
Cuts at: 1870 2810 1870
Size: 940 4545
XmnI GAAnn'nnTTC
Cuts at: 3614 3614
Size: 5485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3293 276
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI