Restriction map for
pFN21AB8157
(Circular) (Six-base) MAPSORT of: pFN21AB8157.seq Check: 6038 from: 1 to: 6352
pFN21AB8157 6352 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4162 278
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3267 3581 3695 1937
Size: 1330 314 114 4594
Fragments arranged by size:
4594 1330 314 114
Acc65I G'GTAC_C
Cuts at: 2551 3673 2551
Size: 1122 5230
AclI AA'CG_TT
Cuts at: 1563 4479 4852 1563
Size: 2916 373 3063
Fragments arranged by size:
3063 2916 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3619 4415 5799 6067 1632
Size: 1987 796 1384 268 1917
Fragments arranged by size:
1987 1917 1384 796 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6155
AflIII A'CryG_T
Cuts at: 1203 5251 6228 1203
Size: 4048 977 1327
Fragments arranged by size:
4048 1327 977
AgeI A'CCGG_T
Cuts at: 5183 5183
Size: 6352
AhdI GACnn_n'nnGTC
Cuts at: 5081 5081
Size: 6352
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6352
AlwNI CAG_nnn'CTG
Cuts at: 4095 5667 4095
Size: 1572 4780
ApaI G_GGCC'C
Cuts at: 3341 3341
Size: 6352
ApaLI G'TGCA_C
Cuts at: 4409 5565 4409
Size: 1156 5196
ApoI r'AATT_y
Cuts at: 1493 2377 2563 3082 3092 3661 3887 4039 6148
Size: 884 186 519 10 569 226 152 2109
Cuts at: 6148 1493
Size: 1697
Fragments arranged by size:
2109 1697 884 569 519 226 186 152
10
AseI AT'TA_AT
Cuts at: 160 1032 4906 160
Size: 872 3874 1606
Fragments arranged by size:
3874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
AvaI C'yCGr_G
Cuts at: 1870 1943 3677 1870
Size: 73 1734 4545
Fragments arranged by size:
4545 1734 73
AvrII C'CTAG_G
Cuts at: 2135 2135
Size: 6352
BamHI G'GATC_C
Cuts at: 3682 4049 3682
Size: 367 5985
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 3191 3673 5128 618
Size: 325 217 627 764 640 482 1455 1842
Fragments arranged by size:
1842 1455 764 640 627 482 325 217
BanII G_rGCy'C
Cuts at: 1391 3341 3671 1391
Size: 1950 330 4072
Fragments arranged by size:
4072 1950 330
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3363 928
Size: 971 1464 3917
Fragments arranged by size:
3917 1464 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4543 4577 1903
Size: 34 2606 34 3678
Fragments arranged by size:
3678 2606 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2866 4244 5454 2866
Size: 1378 1210 3764
Fragments arranged by size:
3764 1378 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6352
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4963 136
Size: 107 122 71 631 605 3291 1525
Fragments arranged by size:
3291 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2545 6347 2545
Size: 3802 2550
BlpI GC'TnA_GC
Cuts at: 2196 3769 2196
Size: 1573 4779
Bme1580I G_kGCm'C
Cuts at: 1790 3341 4413 5569 1790
Size: 1551 1072 1156 2573
Fragments arranged by size:
2573 1551 1156 1072
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6352
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4083 5041 442
Size: 416 53 83 399 24 2666 958 1753
Fragments arranged by size:
2666 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5012 1239
Size: 3773 2579
Bpu10I CC'TnA_GC
Cuts at: 2201 3514 2201
Size: 1313 5039
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4477 5342 5640 4477
Size: 865 298 5189
Fragments arranged by size:
5189 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5015 882
Size: 617 3516 2219
Fragments arranged by size:
3516 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4143 493
Size: 711 2939 2702
Fragments arranged by size:
2939 2702 711
BsaBI GATnn'nnATC
Cuts at: 2909 6346 2909
Size: 3437 2915
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4159 4541 5176 275
Size: 53 83 186 936 2626 382 635 1451
Fragments arranged by size:
2626 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3374 4785 5183 5457 5604 6015 3374
Size: 1411 398 274 147 411 3711
Fragments arranged by size:
3711 1411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3223 3253 3536 3566 1178
Size: 30 165 30 1820 30 283 30 3964
Fragments arranged by size:
3964 1820 283 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2944 3225 3228 3464 3572 1186
Size: 1758 281 3 236 108 3966
Fragments arranged by size:
3966 1758 281 236 108 3
BseYI C'CCAG_C
Cuts at: 5555 6051 5555
Size: 496 5856
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5779
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3802 4563 5591 1540
Size: 275 186 1801 761 1028 2301
Fragments arranged by size:
2301 1801 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2167 4413 4498 5569 2167
Size: 2246 85 1071 2950
Fragments arranged by size:
2950 2246 1071 85
BsmI GAATG_Cn'
Cuts at: 2683 3863 3956 2683
Size: 1180 93 5079
Fragments arranged by size:
5079 1180 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2167 3341 3671 4413 4498 5569 1391
Size: 399 377 1174 330 742 85 1071 2174
Fragments arranged by size:
2174 1174 1071 742 399 377 330 85
BspEI T'CCGG_A
Cuts at: 6015 6015
Size: 6352
BspHI T'CATG_A
Cuts at: 4241 5971 4241
Size: 1730 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 3560 3707 844
Size: 1210 1506 147 3489
Fragments arranged by size:
3489 1506 1210 147
BsrBI CCG'CTC
Cuts at: 1394 1823 4239 1394
Size: 429 2416 3507
Fragments arranged by size:
3507 2416 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4847 5021 65
Size: 1183 3599 174 1396
Fragments arranged by size:
3599 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4996 5183 1700
Size: 110 3186 187 2869
Fragments arranged by size:
3186 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6352
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6352
BssSI C'ACGA_G
Cuts at: 4412 5424 4412
Size: 1012 5340
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2046 794
Size: 1252 5100
BstBI TT'CG_AA
Cuts at: 5156 5156
Size: 6352
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6352
BstXI CCAn_nnnn'nTGG
Cuts at: 3137 3137
Size: 6352
BstYI r'GATC_y
Cuts at: 1972 2458 2545 3682 4049 4434 4451 5903 5989
Size: 486 87 1137 367 385 17 1452 86
Cuts at: 5989 6001 6347 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1137 486 385 367 346 87
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2481 2481
Size: 6352
BtgI C'CryG_G
Cuts at: 513 1065 2859 513
Size: 552 1794 4006
Fragments arranged by size:
4006 1794 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5613
Fragments arranged by size:
5613 683 56
BtsI GCAGTG_nn'
Cuts at: 3868 4662 4682 3868
Size: 794 20 5538
Fragments arranged by size:
5538 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6317
DraI TTT'AAA
Cuts at: 3656 3656
Size: 6352
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5359 809
Size: 507 4043 1802
Fragments arranged by size:
4043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3348 3799 4688
Size: 54 1425 50 275 18 1518 451 889
Cuts at: 4688 6317 8
Size: 1629 43
Fragments arranged by size:
1629 1518 1425 889 451 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3799 1537
Size: 275 1987 4090
Fragments arranged by size:
4090 1987 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 2484 3112 3528 3609 4282 1353
Size: 834 297 628 416 81 673 3423
Fragments arranged by size:
3423 834 673 628 416 297 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4939 5313 5459 387
Size: 1064 138 3350 374 146 1280
Fragments arranged by size:
3350 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3619 4415 5012 5799 6067 1239
Size: 393 1987 796 597 787 268 1524
Fragments arranged by size:
1987 1524 796 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3029 3029
Size: 6352
EcoO109I rG'GnC_Cy
Cuts at: 2719 3337 3338 2719
Size: 618 1 5733
Fragments arranged by size:
5733 618 1
EcoRI G'AATT_C
Cuts at: 2377 3082 3661 4039 2377
Size: 705 579 378 4690
Fragments arranged by size:
4690 705 579 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2738 2738
Size: 6352
FspI TGC'GCA
Cuts at: 4858 4858
Size: 6352
HaeII r_GCGC'y
Cuts at: 1678 5499 1678
Size: 3821 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3223 3255 3536 3568 4993
Size: 32 135 32 1651 32 281 32 1425
Cuts at: 4993 5025 5067 5099 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1651 1425 281 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3575 3696 3942 669
Size: 988 281 1637 121 246 3079
Fragments arranged by size:
3079 1637 988 281 246 121
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6352
HpaI GTT'AAC
Cuts at: 3942 3942
Size: 6352
KpnI G_GTAC'C
Cuts at: 2555 3677 2555
Size: 1122 5230
MfeI C'AATT_G
Cuts at: 3951 3951
Size: 6352
MluI A'CGCG_T
Cuts at: 6228 6228
Size: 6352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2427 2755 5466 5650 1305
Size: 1122 328 2711 184 2007
Fragments arranged by size:
2711 2007 1122 328 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3350 10
Size: 54 1425 343 1518 3012
Fragments arranged by size:
3012 1518 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4310 4669 4828 518
Size: 648 3144 359 159 2042
Fragments arranged by size:
3144 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3768 4445 5593 5838 1606
Size: 2162 677 1148 245 2120
Fragments arranged by size:
2162 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2859 513
Size: 552 1794 4006
Fragments arranged by size:
4006 1794 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6352
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6352
NotI GC'GGCC_GC
Cuts at: 3799 3799
Size: 6352
NspI r_CATG'y
Cuts at: 1132 1229 2881 3523 3710 5255 1132
Size: 97 1652 642 187 1545 2229
Fragments arranged by size:
2229 1652 1545 642 187 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6352
PciI A'CATG_T
Cuts at: 5251 5251
Size: 6352
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6352
PmeI GTTT'AAAC
Cuts at: 3656 3656
Size: 6352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 5934 5966 4421
Size: 32 1481 32 4807
Fragments arranged by size:
4807 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6352
PsiI TTA'TAA
Cuts at: 3922 3922
Size: 6352
PspOMI G'GGCC_C
Cuts at: 3337 3337
Size: 6352
PstI C_TGCA'G
Cuts at: 830 3532 3704 830
Size: 2702 172 3478
Fragments arranged by size:
3478 2702 172
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6352
SalI G'TCGA_C
Cuts at: 1936 3694 1936
Size: 1758 4594
SapI GCTCTTCn'nnn_
Cuts at: 2187 2187
Size: 6352
SbfI CC_TGCA'GG
Cuts at: 3704 3704
Size: 6352
ScaI AGT'ACT
Cuts at: 3054 4600 6312 3054
Size: 1546 1712 3094
Fragments arranged by size:
3094 1712 1546
SfcI C'TryA_G
Cuts at: 826 1045 3076 3528 3564 3700 4835 5516 5707
Size: 219 2031 452 36 136 1135 681 191
Cuts at: 5707 826
Size: 1471
Fragments arranged by size:
2031 1471 1135 681 452 219 191 136
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6352
SmaI CCC'GGG
Cuts at: 1872 3679 1872
Size: 1807 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4456 5357 5619 820
Size: 197 926 2513 901 262 1553
Fragments arranged by size:
2513 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4143 493
Size: 3650 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6352
SphI G_CATG'C
Cuts at: 1132 2881 3710 1132
Size: 1749 829 3774
Fragments arranged by size:
3774 1749 829
SspI AAT'ATT
Cuts at: 5 52 4276 5
Size: 47 4224 2081
Fragments arranged by size:
4224 2081 47
StuI AGG'CCT
Cuts at: 1517 2014 1517
Size: 497 5855
StyI C'CwwG_G
Cuts at: 513 1065 2135 2859 2892 3791 513
Size: 552 1070 724 33 899 3074
Fragments arranged by size:
3074 1070 899 724 552 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4379 4396 4549 4734 5179 325
Size: 1255 2799 17 153 185 445 1498
Fragments arranged by size:
2799 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2391 3052 3638
Size: 274 80 33 51 1443 414 661 586
Cuts at: 3638 4598 6310 96
Size: 960 1712 138
Fragments arranged by size:
1712 1443 960 661 586 414 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6312 6344 6312
Size: 32 6320
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6352
XbaI T'CTAG_A
Cuts at: 3688 3688
Size: 6352
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6352
XmaI C'CCGG_G
Cuts at: 1870 3677 1870
Size: 1807 4545
XmnI GAAnn'nnTTC
Cuts at: 4481 4481
Size: 6352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4160 276
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI