Restriction map for
pFN21AB8156
(Circular) (Six-base) MAPSORT of: pFN21AB8156.seq Check: 5255 from: 1 to: 5917
pFN21AB8156 5917 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2287 2287
Size: 5917
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3012 3727 278
Size: 53 83 186 936 1476 715 2468
Fragments arranged by size:
2468 1476 936 715 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3260 1937
Size: 1323 4594
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 3216 4044 4417 1563
Size: 1653 828 373 3063
Fragments arranged by size:
3063 1653 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 2429 3980 5364 5632 1632
Size: 449 348 1551 1384 268 1917
Fragments arranged by size:
1917 1551 1384 449 348 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 4816 5793 1203
Size: 3613 977 1327
Fragments arranged by size:
3613 1327 977
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 5885
AlwNI CAG_nnn'CTG
Cuts at: 2329 3660 5232 2329
Size: 1331 1572 3014
Fragments arranged by size:
3014 1572 1331
ApaLI G'TGCA_C
Cuts at: 2150 3974 5130 2150
Size: 1824 1156 2937
Fragments arranged by size:
2937 1824 1156
ApoI r'AATT_y
Cuts at: 1493 2141 2363 3050 3091 3226 3452 3604 5713
Size: 648 222 687 41 135 226 152 2109
Cuts at: 5713 1493
Size: 1697
Fragments arranged by size:
2109 1697 687 648 226 222 152 135
41
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2018 2310 3242 1870
Size: 73 75 292 932 4545
Fragments arranged by size:
4545 932 292 75 73
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3238 4693 618
Size: 325 217 627 1451 1455 1842
Fragments arranged by size:
1842 1455 1451 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2186 2781 2886 3236 1391
Size: 795 595 105 350 4072
Fragments arranged by size:
4072 795 595 350 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2220 2423 928
Size: 971 321 203 4422
Fragments arranged by size:
4422 971 321 203
BbvCI CC'TCA_GC
Cuts at: 3021 3021
Size: 5917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5019 3809
Size: 1210 4707
BclI T'GATC_A
Cuts at: 1548 2509 1548
Size: 961 4956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4528 136
Size: 107 122 71 631 605 2856 1525
Fragments arranged by size:
2856 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2060 2708 5912 2060
Size: 648 3204 2065
Fragments arranged by size:
3204 2065 648
BlpI GC'TnA_GC
Cuts at: 2814 3334 2814
Size: 520 5397
Bme1580I G_kGCm'C
Cuts at: 1790 2154 3978 5134 1790
Size: 364 1824 1156 2573
Fragments arranged by size:
2573 1824 1156 364
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2339 3648 4606
Size: 416 53 83 399 24 922 1309 958
Cuts at: 4606 442
Size: 1753
Fragments arranged by size:
1753 1309 958 922 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2625 4577 1239
Size: 1386 1952 2579
Fragments arranged by size:
2579 1952 1386
Bpu10I CC'TnA_GC
Cuts at: 2919 3021 2919
Size: 102 5815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4042 4907 5205 4042
Size: 865 298 4754
Fragments arranged by size:
4754 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4580 882
Size: 617 3081 2219
Fragments arranged by size:
3081 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3708 493
Size: 711 2504 2702
Fragments arranged by size:
2702 2504 711
BsaBI GATnn'nnATC
Cuts at: 2065 2514 5911 2065
Size: 449 3397 2071
Fragments arranged by size:
3397 2071 449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3009 3724 4106 4741
Size: 53 83 186 936 1476 715 382 635
Cuts at: 4741 275
Size: 1451
Fragments arranged by size:
1476 1451 936 715 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4350 4748 5022 5169 5580 4350
Size: 398 274 147 411 4687
Fragments arranged by size:
4687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3161 3191 1178
Size: 30 165 30 1758 30 3904
Fragments arranged by size:
3904 1758 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2876 1186
Size: 868 822 4227
Fragments arranged by size:
4227 868 822
BseYI C'CCAG_C
Cuts at: 2261 5120 5616 2261
Size: 2859 496 2562
Fragments arranged by size:
2859 2562 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3367 4128 5156 1540
Size: 275 186 1366 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 2886 3978 4063 5134 2154
Size: 732 1092 85 1071 2937
Fragments arranged by size:
2937 1092 1071 732 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2186 2781 2886 3236 3978 4063
Size: 399 364 32 595 105 350 742 85
Cuts at: 4063 5134 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 595 399 364 350 105
85 32
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2287 3272 844
Size: 1443 985 3489
Fragments arranged by size:
3489 1443 985
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4412 4586 65
Size: 1183 3164 174 1396
Fragments arranged by size:
3164 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4561 4748 1700
Size: 110 2751 187 2869
Fragments arranged by size:
2869 2751 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 4721 4721
Size: 5917
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 2060 2515 2708 2923 3247 3614 3999 4016
Size: 88 455 193 215 324 367 385 17
Cuts at: 4016 5468 5554 5566 5912 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 455 385 367 346 324 215
193 88 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 513
Size: 552 939 6 4420
Fragments arranged by size:
4420 939 552 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 2165 3433 4227 4247 2165
Size: 1268 794 20 3835
Fragments arranged by size:
3835 1268 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4924 809
Size: 507 3608 1802
Fragments arranged by size:
3608 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3364 4253
Size: 54 1425 50 275 18 177 1357 889
Cuts at: 4253 5882 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1357 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2769 3098 3847 1353
Size: 1416 329 749 3423
Fragments arranged by size:
3423 1416 749 329
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2545 4504 4878 5024 387
Size: 1064 138 956 1959 374 146 1280
Fragments arranged by size:
1959 1280 1064 956 374 146 138
EcoICRI GAG'CTC
Cuts at: 2884 2884
Size: 5917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2429 2625 3980 4577 5364 5632
Size: 393 449 348 196 1355 597 787 268
Cuts at: 5632 1239
Size: 1524
Fragments arranged by size:
1524 1355 787 597 449 393 348 268
196
EcoNI CCTnn'n_nnAGG
Cuts at: 2804 2804
Size: 5917
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2459 2944 2314
Size: 145 485 5287
Fragments arranged by size:
5287 485 145
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 5064 1678
Size: 3386 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2121 2153 2695 2727 4558
Size: 32 135 32 549 32 542 32 1831
Cuts at: 4558 4590 4632 4664 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1831 549 542 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3261 3507 669
Size: 988 281 1323 246 3079
Fragments arranged by size:
3079 1323 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5031 5215 1305
Size: 3726 184 2007
Fragments arranged by size:
3726 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4095
Fragments arranged by size:
4095 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2718 3875 4234 4393 518
Size: 648 1552 1157 359 159 2042
Fragments arranged by size:
2042 1552 1157 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2449 3333 4010 5158 5403 1606
Size: 406 437 884 677 1148 245 2120
Fragments arranged by size:
2120 1148 884 677 437 406 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4426
Fragments arranged by size:
4426 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 2902 3164 3275 4820 1132
Size: 97 1673 262 111 1545 2229
Fragments arranged by size:
2229 1673 1545 262 111 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5917
PfoI T'CCnGG_A
Cuts at: 2499 2499
Size: 5917
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3986 4018 5499 5531 3161
Size: 32 793 32 1481 32 3547
Fragments arranged by size:
3547 1481 793 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PstI C_TGCA'G
Cuts at: 830 2031 3269 830
Size: 1201 1238 3478
Fragments arranged by size:
3478 1238 1201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
SacI G_AGCT'C
Cuts at: 2886 2886
Size: 5917
SacII CC_GC'GG
Cuts at: 2013 2013
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SapI GCTCTTCn'nnn_
Cuts at: 2769 2769
Size: 5917
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 4165 5877 4165
Size: 1712 4205
SfcI C'TryA_G
Cuts at: 826 1045 2027 3265 4400 5081 5272 826
Size: 219 982 1238 1135 681 191 1471
Fragments arranged by size:
1471 1238 1135 982 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 3244 1872
Size: 1372 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2310 4021 4922 5184 820
Size: 197 926 367 1711 901 262 1553
Fragments arranged by size:
1711 1553 926 901 367 262 197
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5917
StyI C'CwwG_G
Cuts at: 513 1065 2004 3356 513
Size: 552 939 1352 3074
Fragments arranged by size:
3074 1352 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3944 3961 4114 4299 4744 325
Size: 1255 2364 17 153 185 445 1498
Fragments arranged by size:
2364 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4163 5875 96
Size: 274 80 33 51 1443 2186 1712 138
Fragments arranged by size:
2186 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5877 5909 5877
Size: 32 5885
Tth111I GACn'n_nGTC
Cuts at: 1316 2135 2422 1316
Size: 819 287 4811
Fragments arranged by size:
4811 819 287
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XhoI C'TCGA_G
Cuts at: 1943 2310 1943
Size: 367 5550
XmaI C'CCGG_G
Cuts at: 1870 3242 1870
Size: 1372 4545
XmnI GAAnn'nnTTC
Cuts at: 2439 2645 4046 2439
Size: 206 1401 4310
Fragments arranged by size:
4310 1401 206
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3010 3725 276
Size: 53 83 186 936 1476 715 2468
Fragments arranged by size:
2468 1476 936 715 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI