Restriction map for
pFN21AB8150
(Circular) (Six-base) MAPSORT of: pFN21AB8150.seq Check: 3802 from: 1 to: 6142
pFN21AB8150 6142 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3109 3485 1937
Size: 1172 376 4594
Fragments arranged by size:
4594 1172 376
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4205 5589 5857 1632
Size: 2573 1384 268 1917
Fragments arranged by size:
2573 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 2575 5041 6018 1203
Size: 1372 2466 977 1327
Fragments arranged by size:
2466 1372 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2230 1184
Size: 1046 5096
AlwNI CAG_nnn'CTG
Cuts at: 2282 2675 3885 5457 2282
Size: 393 1210 1572 2967
Fragments arranged by size:
2967 1572 1210 393
ApaI G_GGCC'C
Cuts at: 2648 2648
Size: 6142
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 2688 2711 3049 3451 3677 3829 5938 1493
Size: 1195 23 338 402 226 152 2109 1697
Fragments arranged by size:
2109 1697 1195 402 338 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 3207 4696 160
Size: 872 2175 1489 1606
Fragments arranged by size:
2175 1606 1489 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2199 3467 1870
Size: 73 256 1268 4545
Fragments arranged by size:
4545 1268 256 73
BamHI G'GATC_C
Cuts at: 2195 3472 3839 2195
Size: 1277 367 4498
Fragments arranged by size:
4498 1277 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3463 4918 618
Size: 325 217 627 1676 1455 1842
Fragments arranged by size:
1842 1676 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2648 2766 3461 1391
Size: 1257 118 695 4072
Fragments arranged by size:
4072 1257 695 118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2163 2259 928
Size: 971 264 96 4811
Fragments arranged by size:
4811 971 264 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4753 136
Size: 107 122 71 631 605 3081 1525
Fragments arranged by size:
3081 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6137 6137
Size: 6142
BlpI GC'TnA_GC
Cuts at: 3559 3559
Size: 6142
Bme1580I G_kGCm'C
Cuts at: 1790 2648 4203 5359 1790
Size: 858 1555 1156 2573
Fragments arranged by size:
2573 1555 1156 858
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3335 3873 4831
Size: 416 53 83 399 24 1918 538 958
Cuts at: 4831 442
Size: 1753
Fragments arranged by size:
1918 1753 958 538 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3266 1056
Size: 2210 3932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2652 3257 4802 1239
Size: 1413 605 1545 2579
Fragments arranged by size:
2579 1545 1413 605
Bpu10I CC'TnA_GC
Cuts at: 2081 2427 2081
Size: 346 5796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2309 2810 4267 5132 5430 2309
Size: 501 1457 865 298 3021
Fragments arranged by size:
3021 1457 865 501 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2911 4805 882
Size: 617 1412 1894 2219
Fragments arranged by size:
2219 1894 1412 617
BsaAI yAC'GTr
Cuts at: 493 1204 2462 3933 493
Size: 711 1258 1471 2702
Fragments arranged by size:
2702 1471 1258 711
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3949 4331 4966 275
Size: 53 83 186 936 2416 382 635 1451
Fragments arranged by size:
2416 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4575 4973 5247 5394 5805 4575
Size: 398 274 147 411 4912
Fragments arranged by size:
4912 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2547 2577 1178
Size: 30 165 30 1144 30 4743
Fragments arranged by size:
4743 1144 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2774 3254 1186
Size: 1588 480 4074
Fragments arranged by size:
4074 1588 480
BseYI C'CCAG_C
Cuts at: 2057 5345 5841 2057
Size: 3288 496 2358
Fragments arranged by size:
3288 2358 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2608 2766 4203 4288 5359 2608
Size: 158 1437 85 1071 3391
Fragments arranged by size:
3391 1437 1071 158 85
BsmI GAATG_Cn'
Cuts at: 3003 3653 3746 3003
Size: 650 93 5399
Fragments arranged by size:
5399 650 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2608 2648 2766 3461 4203 4288 5359
Size: 399 818 40 118 695 742 85 1071
Cuts at: 5359 1391
Size: 2174
Fragments arranged by size:
2174 1071 818 742 695 399 118 85
40
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 4031 5761 4031
Size: 1730 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3497 844
Size: 2653 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4029 1394
Size: 429 2206 3507
Fragments arranged by size:
3507 2206 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4637 4811 65
Size: 1183 3389 174 1396
Fragments arranged by size:
3389 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4786 4973 1700
Size: 110 2976 187 2869
Fragments arranged by size:
2976 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 2601 4202 5214 2601
Size: 1601 1012 3529
Fragments arranged by size:
3529 1601 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 3320 4946 3320
Size: 1626 4516
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstYI r'GATC_y
Cuts at: 1972 2195 2279 2736 3472 3839 4224 4241 5693
Size: 223 84 457 736 367 385 17 1452
Cuts at: 5693 5779 5791 6137 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 736 457 385 367 346 223
86 84 17 12
Bsu36I CC'TnA_GG
Cuts at: 2676 2676
Size: 6142
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4651
Fragments arranged by size:
4651 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 2412 3658 4452 4472 2412
Size: 1246 794 20 4082
Fragments arranged by size:
4082 1246 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2388 2423 529
Size: 35 1824 35 4248
Fragments arranged by size:
4248 1824 35 35
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3589 4478 6107
Size: 54 1425 50 275 18 1759 889 1629
Cuts at: 6107 8
Size: 43
Fragments arranged by size:
1759 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2697 2961 4072 1353
Size: 1344 264 1111 3423
Fragments arranged by size:
3423 1344 1111 264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4729 5103 5249 387
Size: 1064 138 3140 374 146 1280
Fragments arranged by size:
3140 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2764 2764
Size: 6142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2652 3257 4205 4802 5589 5857 1239
Size: 393 1020 605 948 597 787 268 1524
Fragments arranged by size:
1524 1020 948 787 605 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3063 3063
Size: 6142
EcoO109I rG'GnC_Cy
Cuts at: 2644 3138 2644
Size: 494 5648
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
FspI TGC'GCA
Cuts at: 3023 4648 3023
Size: 1625 4517
FspAI rTGC'GCAy
Cuts at: 3023 3023
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 5289 1678
Size: 3611 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3125 3157 3193 3225 4783
Size: 32 135 32 1553 32 36 32 1558
Cuts at: 4783 4815 4857 4889 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1558 1553 135 42 36 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3486 3732 669
Size: 988 281 1548 246 3079
Fragments arranged by size:
3079 1548 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 2821 3741 2821
Size: 920 5222
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2919 5256 5440 1305
Size: 1614 2337 184 2007
Fragments arranged by size:
2337 2007 1614 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4320
Fragments arranged by size:
4320 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4100 4459 4618 518
Size: 648 2934 359 159 2042
Fragments arranged by size:
2934 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2129 2267 3302 3558 4235 5383 5628 1606
Size: 523 138 1035 256 677 1148 245 2120
Fragments arranged by size:
2120 1148 1035 677 523 256 245 138
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4651
Fragments arranged by size:
4651 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 3262 1052
Size: 2210 3932
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 2092 2356 3500 5045 1132
Size: 97 863 264 1144 1545 2229
Fragments arranged by size:
2229 1545 1144 863 264 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6142
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6142
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PmlI CAC'GTG
Cuts at: 2462 2462
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PspOMI G'GGCC_C
Cuts at: 2644 2644
Size: 6142
PstI C_TGCA'G
Cuts at: 830 2407 3037 3494 830
Size: 1577 630 457 3478
Fragments arranged by size:
3478 1577 630 457
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2129 2267 3302 2129
Size: 138 1035 4969
Fragments arranged by size:
4969 1035 138
SacI G_AGCT'C
Cuts at: 2766 2766
Size: 6142
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SfcI C'TryA_G
Cuts at: 826 1045 2403 3033 3490 4625 5306 5497 826
Size: 219 1358 630 457 1135 681 191 1471
Fragments arranged by size:
1471 1358 1135 681 630 457 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 3469 1872
Size: 1597 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2288 2789 4246 5147 5409 820
Size: 197 926 345 501 1457 901 262 1553
Fragments arranged by size:
1553 1457 926 901 501 345 262 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 2878 3251 152
Size: 2726 373 3043
Fragments arranged by size:
3043 2726 373
SphI G_CATG'C
Cuts at: 1132 2092 3500 1132
Size: 960 1408 3774
Fragments arranged by size:
3774 1408 960
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6142
StyI C'CwwG_G
Cuts at: 513 1065 2004 2525 2901 3581 513
Size: 552 939 521 376 680 3074
Fragments arranged by size:
3074 939 680 552 521 376
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 4169 4186 4339 4524 4969 325
Size: 1255 417 2172 17 153 185 445 1498
Fragments arranged by size:
2172 1498 1255 445 417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2875 4388 6100
Size: 274 80 33 51 1443 898 1513 1712
Cuts at: 6100 96
Size: 138
Fragments arranged by size:
1712 1513 1443 898 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2088 2120 2187 2219 2547 2579 2801 2833 3165
Size: 32 67 32 328 32 222 32 332
Cuts at: 3165 3197 3327 3359 6102 6134 2088
Size: 32 130 32 2743 32 2096
Fragments arranged by size:
2743 2096 332 328 222 130 67 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 3467 1870
Size: 1597 4545
XmnI GAAnn'nnTTC
Cuts at: 4271 4271
Size: 6142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV
FalI FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI