Restriction map for
pFN21AB8148
(Circular) (Six-base) MAPSORT of: pFN21AB8148.seq Check: 2065 from: 1 to: 6334
pFN21AB8148 6334 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3677 1937
Size: 1740 4594
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1999 2693 3441 3501 3531 3594 4397 5781
Size: 367 694 748 60 30 63 803 1384
Cuts at: 5781 6049 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 803 748 694 367 268 63
60 30
AfeI AGC'GCT
Cuts at: 2988 3040 2988
Size: 52 6282
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 5233 6210 1203
Size: 4030 977 1327
Fragments arranged by size:
4030 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 2254 2917 5063 2254
Size: 663 2146 3525
Fragments arranged by size:
3525 2146 663
AleI CACnn'nnGTG
Cuts at: 1166 3215 1166
Size: 2049 4285
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6334
AlwNI CAG_nnn'CTG
Cuts at: 3010 4077 5649 3010
Size: 1067 1572 3695
Fragments arranged by size:
3695 1572 1067
ApaLI G'TGCA_C
Cuts at: 2075 2327 2681 4391 5547 2075
Size: 252 354 1710 1156 2862
Fragments arranged by size:
2862 1710 1156 354 252
ApoI r'AATT_y
Cuts at: 1493 3643 3869 4021 6130 1493
Size: 2150 226 152 2109 1697
Fragments arranged by size:
2150 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2630 3659 1870
Size: 73 127 560 1029 4545
Fragments arranged by size:
4545 1029 560 127 73
BamHI G'GATC_C
Cuts at: 3664 4031 3664
Size: 367 5967
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 2597 3655 5110 618
Size: 325 217 627 350 460 1058 1455 1842
Fragments arranged by size:
1842 1455 1058 627 460 350 325 217
BanII G_rGCy'C
Cuts at: 1391 2631 3251 3414 3653 1391
Size: 1240 620 163 239 4072
Fragments arranged by size:
4072 1240 620 239 163
BbeI G_GCGC'C
Cuts at: 2141 2601 2141
Size: 460 5874
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 928
Size: 971 240 5123
Fragments arranged by size:
5123 971 240
BbvCI CC'TCA_GC
Cuts at: 2832 3393 2832
Size: 561 5773
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4525 4559 1903
Size: 34 2588 34 3678
Fragments arranged by size:
3678 2588 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4226 5436 4226
Size: 1210 5124
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2606 4945 136
Size: 107 122 71 631 605 934 2339 1525
Fragments arranged by size:
2339 1525 934 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2730 6329 2730
Size: 3599 2735
BlpI GC'TnA_GC
Cuts at: 2116 2552 3751 2116
Size: 436 1199 4699
Fragments arranged by size:
4699 1199 436
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2331 2685 4395 5551 1790
Size: 289 252 354 1710 1156 2573
Fragments arranged by size:
2573 1710 1156 354 289 252
BmgBI CAC'GTC
Cuts at: 1318 2749 1318
Size: 1431 4903
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2196 4065 5023
Size: 416 53 83 399 24 779 1869 958
Cuts at: 5023 442
Size: 1753
Fragments arranged by size:
1869 1753 958 779 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3433 3433
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 2110 2164 2228 2270 2357 2468 2525
Size: 829 42 54 64 42 87 111 57
Cuts at: 2525 3011 3032 4994 1239
Size: 486 21 1962 2579
Fragments arranged by size:
2579 1962 829 486 111 87 64 57
54 42 42 21
Bpu10I CC'TnA_GC
Cuts at: 2222 2832 3393 2222
Size: 610 561 5163
Fragments arranged by size:
5163 610 561
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2762 2924 4459 5324 5622 2237
Size: 525 162 1535 865 298 2949
Fragments arranged by size:
2949 1535 865 525 298 162
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2504 3188 4997 882
Size: 617 1005 684 1809 2219
Fragments arranged by size:
2219 1809 1005 684 617
BsaAI yAC'GTr
Cuts at: 493 1204 2059 4125 493
Size: 711 855 2066 2702
Fragments arranged by size:
2702 2066 855 711
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2138 2565 2598
Size: 53 83 186 936 533 72 427 33
Cuts at: 2598 2892 4141 4523 5158 275
Size: 294 1249 382 635 1451
Fragments arranged by size:
1451 1249 936 635 533 427 382 294
186 83 72 53 33
BsaWI w'CCGG_w
Cuts at: 4767 5165 5439 5586 5997 4767
Size: 398 274 147 411 5104
Fragments arranged by size:
5104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 1178
Size: 30 165 30 621 30 5458
Fragments arranged by size:
5458 621 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2167 2522 2558 2705 3119 3206 3365 3449
Size: 981 355 36 147 414 87 159 84
Cuts at: 3449 3452 3455 3485 3593 3596 1186
Size: 3 3 30 108 3 3924
Fragments arranged by size:
3924 981 414 355 159 147 108 87
84 36 30 3 3 3
BseYI C'CCAG_C
Cuts at: 3602 5537 6033 3602
Size: 1935 496 3903
Fragments arranged by size:
3903 1935 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5761
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3784 4545 5573 1540
Size: 275 186 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2331 2631 2685 3251 4395 4480 5551 2079
Size: 252 300 54 566 1144 85 1071 2862
Fragments arranged by size:
2862 1144 1071 566 300 252 85 54
BsmI GAATG_Cn'
Cuts at: 3845 3938 3845
Size: 93 6241
BsmBI CGTCTCn'nnnn_
Cuts at: 2057 2057
Size: 6334
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2331 2631 2685 3251 3414 3653
Size: 399 289 252 300 54 566 163 239
Cuts at: 3653 4395 4480 5551 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 566 399 300 289 252
239 163 85 54
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 4223 5953 4223
Size: 1730 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2448 2532 3689 844
Size: 1604 84 1157 3489
Fragments arranged by size:
3489 1604 1157 84
BsrBI CCG'CTC
Cuts at: 1394 1823 3315 4221 1394
Size: 429 1492 906 3507
Fragments arranged by size:
3507 1492 906 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4829 5003 65
Size: 1183 3581 174 1396
Fragments arranged by size:
3581 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2140 2574 4978 5165 1700
Size: 110 330 434 2404 187 2869
Fragments arranged by size:
2869 2404 434 330 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 2531 2588 2790 2899 1925
Size: 606 57 202 109 5360
Fragments arranged by size:
5360 606 202 109 57
BssSI C'ACGA_G
Cuts at: 2330 4394 5406 2330
Size: 2064 1012 3258
Fragments arranged by size:
3258 2064 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6334
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstXI CCAn_nnnn'nTGG
Cuts at: 2953 3260 2953
Size: 307 6027
BstYI r'GATC_y
Cuts at: 1972 2430 2715 2730 3664 4031 4416 4433 5885
Size: 458 285 15 934 367 385 17 1452
Cuts at: 5885 5971 5983 6329 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 934 458 385 367 346 285
86 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2736 513
Size: 552 1671 4111
Fragments arranged by size:
4111 1671 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5595
Fragments arranged by size:
5595 683 56
BtsI GCAGTG_nn'
Cuts at: 3850 4644 4664 3850
Size: 794 20 5520
Fragments arranged by size:
5520 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 809
Size: 507 4025 1802
Fragments arranged by size:
4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2808 3262 3781
Size: 54 1425 50 275 18 978 454 519
Cuts at: 3781 4670 6299 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 978 889 519 454 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2535 2682 3450 3462 3477 3498 3570 4264
Size: 1182 147 768 12 15 21 72 694
Cuts at: 4264 1353
Size: 3423
Fragments arranged by size:
3423 1182 768 694 147 72 21 15
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2468 2758 4921 5295 5441 387
Size: 1064 138 879 290 2163 374 146 1280
Fragments arranged by size:
2163 1280 1064 879 374 290 146 138
EcoICRI GAG'CTC
Cuts at: 2629 3249 2629
Size: 620 5714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2068 2110 2164 2228 2270 2357
Size: 393 367 69 42 54 64 42 87
Cuts at: 2357 2468 2525 2693 3011 3032 3441 3501 3531
Size: 111 57 168 318 21 409 60 30
Cuts at: 3531 3594 4397 4994 5781 6049 1239
Size: 63 803 597 787 268 1524
Fragments arranged by size:
1524 803 787 597 409 393 367 318
268 168 111 87 69 64 63 60
57 54 42 42 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 2225
Size: 6334
EcoO109I rG'GnC_Cy
Cuts at: 2290 2353 2290
Size: 63 6271
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 6334
FspI TGC'GCA
Cuts at: 2093 2519 4840 2093
Size: 426 2321 3587
Fragments arranged by size:
3587 2321 426
HaeII r_GCGC'y
Cuts at: 1678 2056 2141 2417 2483 2601 2615 2877 2990
Size: 378 85 276 66 118 14 262 113
Cuts at: 2990 3042 5481 1678
Size: 52 2439 2531
Fragments arranged by size:
2531 2439 378 276 262 118 113 85
66 52 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2188 2220 2240 2272 2648
Size: 32 135 32 616 32 20 32 376
Cuts at: 2648 2680 2903 2917 2935 2949 4975 5007 5049
Size: 32 223 14 18 14 2026 32 42
Cuts at: 5049 5081 1373
Size: 32 2626
Fragments arranged by size:
2626 2026 616 376 223 135 42 32
32 32 32 32 32 32 20 18
14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3678 3924 669
Size: 988 281 1740 246 3079
Fragments arranged by size:
3079 1740 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6334
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KasI G'GCGC_C
Cuts at: 2137 2597 2137
Size: 460 5874
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5448 5632 1305
Size: 4143 184 2007
Fragments arranged by size:
4143 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4512
Fragments arranged by size:
4512 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3215 4292 4651 4810 518
Size: 648 2049 1077 359 159 2042
Fragments arranged by size:
2049 2042 1077 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2097 3001 3479 3750 4427 5575 5820 1606
Size: 491 904 478 271 677 1148 245 2120
Fragments arranged by size:
2120 1148 904 677 491 478 271 245
NaeI GCC'GGC
Cuts at: 2576 2576
Size: 6334
NarI GG'CG_CC
Cuts at: 2138 2598 2138
Size: 460 5874
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5782
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NgoMIV G'CCGG_C
Cuts at: 2574 2574
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 2824 2970 3692 5237 1132
Size: 97 1595 146 722 1545 2229
Fragments arranged by size:
2229 1595 1545 722 146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6334
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6334
PfoI T'CCnGG_A
Cuts at: 2286 2355 2286
Size: 69 6265
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 4403 4435 5916 5948 2825
Size: 32 1546 32 1481 32 3211
Fragments arranged by size:
3211 1546 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2290 2353 2290
Size: 63 6271
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PstI C_TGCA'G
Cuts at: 830 2529 2709 3006 3686 830
Size: 1699 180 297 680 3478
Fragments arranged by size:
3478 1699 680 297 180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 3001 3479 3001
Size: 478 5856
SacI G_AGCT'C
Cuts at: 2631 3251 2631
Size: 620 5714
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SbfI CC_TGCA'GG
Cuts at: 2529 3686 2529
Size: 1157 5177
ScaI AGT'ACT
Cuts at: 4582 6294 4582
Size: 1712 4622
SfcI C'TryA_G
Cuts at: 826 1045 2101 2525 2705 3002 3682 4817 5498
Size: 219 1056 424 180 297 680 1135 681
Cuts at: 5498 5689 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1056 681 680 424 297 219
191 180
SfoI GGC'GCC
Cuts at: 2139 2599 2139
Size: 460 5874
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 2140 1700
Size: 440 5894
SmaI CCC'GGG
Cuts at: 1872 2072 3661 1872
Size: 200 1589 4545
Fragments arranged by size:
4545 1589 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 2630 2777 2939 4438 5339
Size: 197 926 309 378 147 162 1499 901
Cuts at: 5339 5601 820
Size: 262 1553
Fragments arranged by size:
1553 1499 926 901 378 309 262 197
162 147
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 2970 3692 1132
Size: 1838 722 3774
Fragments arranged by size:
3774 1838 722
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6334
StyI C'CwwG_G
Cuts at: 513 1065 2907 3387 3402 3498 3773 513
Size: 552 1842 480 15 96 275 3074
Fragments arranged by size:
3074 1842 552 480 275 96 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2882 4361 4378 4531 4716 5161 325
Size: 1255 1302 1479 17 153 185 445 1498
Fragments arranged by size:
1498 1479 1302 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4580 6292 96
Size: 274 80 33 51 1443 2603 1712 138
Fragments arranged by size:
2603 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6294 6326 6294
Size: 32 6302
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XhoI C'TCGA_G
Cuts at: 1943 2630 1943
Size: 687 5647
XmaI C'CCGG_G
Cuts at: 1870 2070 3659 1870
Size: 200 1589 4545
Fragments arranged by size:
4545 1589 200
XmnI GAAnn'nnTTC
Cuts at: 4463 4463
Size: 6334
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI