Restriction map for
pFN21AB8144
(Circular) (Six-base) MAPSORT of: pFN21AB8144.seq Check: 1888 from: 1 to: 5512
pFN21AB8144 5512 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2036 2036
Size: 5512
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 278
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2855 1937
Size: 918 4594
Acc65I G'GTAC_C
Cuts at: 2833 2833
Size: 5512
AclI AA'CG_TT
Cuts at: 1563 3639 4012 1563
Size: 2076 373 3063
Fragments arranged by size:
3063 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 3575 4959 5227 1632
Size: 407 1536 1384 268 1917
Fragments arranged by size:
1917 1536 1384 407 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5315
AflIII A'CryG_T
Cuts at: 1203 4411 5388 1203
Size: 3208 977 1327
Fragments arranged by size:
3208 1327 977
AgeI A'CCGG_T
Cuts at: 4343 4343
Size: 5512
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 2560 1166
Size: 1394 4118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5512
AlwNI CAG_nnn'CTG
Cuts at: 3255 4827 3255
Size: 1572 3940
ApaLI G'TGCA_C
Cuts at: 3569 4725 3569
Size: 1156 4356
ApoI r'AATT_y
Cuts at: 1493 2265 2663 2821 3047 3199 5308 1493
Size: 772 398 158 226 152 2109 1697
Fragments arranged by size:
2109 1697 772 398 226 158 152
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2140 2837 1870
Size: 73 197 697 4545
Fragments arranged by size:
4545 697 197 73
BamHI G'GATC_C
Cuts at: 2842 3209 2842
Size: 367 5145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2833 4288 618
Size: 325 217 627 1046 1455 1842
Fragments arranged by size:
1842 1455 1046 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2831 1391
Size: 1440 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2362 928
Size: 971 463 4078
Fragments arranged by size:
4078 971 463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2531 2565 3703 3737 1903
Size: 34 50 34 510 34 1138 34 3678
Fragments arranged by size:
3678 1138 510 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4614 3404
Size: 1210 4302
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2115 4123 136
Size: 107 122 71 631 605 443 2008 1525
Fragments arranged by size:
2008 1525 631 605 443 122 107 71
BglII A'GATC_T
Cuts at: 2635 5507 2635
Size: 2872 2640
BlpI GC'TnA_GC
Cuts at: 2929 2929
Size: 5512
Bme1580I G_kGCm'C
Cuts at: 1790 3573 4729 1790
Size: 1783 1156 2573
Fragments arranged by size:
2573 1783 1156
BmgBI CAC'GTC
Cuts at: 1318 2049 2562 1318
Size: 731 513 4268
Fragments arranged by size:
4268 731 513
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2169 3243 4201
Size: 416 53 83 399 24 752 1074 958
Cuts at: 4201 442
Size: 1753
Fragments arranged by size:
1753 1074 958 752 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2298 2298
Size: 5512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2172 4172 1239
Size: 933 2000 2579
Fragments arranged by size:
2579 2000 933
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3637 4502 4800 3637
Size: 865 298 4349
Fragments arranged by size:
4349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2586 4175 882
Size: 617 1087 1589 2219
Fragments arranged by size:
2219 1589 1087 617
BsaAI yAC'GTr
Cuts at: 493 1204 3303 493
Size: 711 2099 2702
Fragments arranged by size:
2702 2099 711
BsaBI GATnn'nnATC
Cuts at: 5506 5506
Size: 5512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3319 3701 4336 275
Size: 53 83 186 936 1786 382 635 1451
Fragments arranged by size:
1786 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3945 4343 4617 4764 5175 3945
Size: 398 274 147 411 4282
Fragments arranged by size:
4282 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5287
Fragments arranged by size:
5287 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5512
BseYI C'CCAG_C
Cuts at: 2011 4715 5211 2011
Size: 2704 496 2312
Fragments arranged by size:
2704 2312 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2571 1163
Size: 573 835 4104
Fragments arranged by size:
4104 835 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2962 3723 4751 1540
Size: 275 186 961 761 1028 2301
Fragments arranged by size:
2301 1028 961 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3573 3658 4729 3573
Size: 85 1071 4356
Fragments arranged by size:
4356 1071 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2831 3573 3658 4729 1391
Size: 399 1041 742 85 1071 2174
Fragments arranged by size:
2174 1071 1041 742 399 85
BspEI T'CCGG_A
Cuts at: 5175 5175
Size: 5512
BspHI T'CATG_A
Cuts at: 3401 5131 3401
Size: 1730 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2036 2867 844
Size: 1192 831 3489
Fragments arranged by size:
3489 1192 831
BsrBI CCG'CTC
Cuts at: 1394 1823 3399 1394
Size: 429 1576 3507
Fragments arranged by size:
3507 1576 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2098 4007 4181 65
Size: 1183 850 1909 174 1396
Fragments arranged by size:
1909 1396 1183 850 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4156 4343 1700
Size: 110 2346 187 2869
Fragments arranged by size:
2869 2346 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5512
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5512
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2037 794
Size: 1243 4269
BstBI TT'CG_AA
Cuts at: 4316 4316
Size: 5512
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5512
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2809 2011
Size: 798 4714
BstYI r'GATC_y
Cuts at: 1972 2085 2635 2842 3209 3594 3611 5063 5149
Size: 113 550 207 367 385 17 1452 86
Cuts at: 5149 5161 5507 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 550 385 367 346 207 113
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4960
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4773
Fragments arranged by size:
4773 683 56
BtsI GCAGTG_nn'
Cuts at: 2415 3028 3822 3842 2415
Size: 613 794 20 4085
Fragments arranged by size:
4085 794 613 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2368 2816 2368
Size: 448 5064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2050 4519 809
Size: 507 734 2469 1802
Fragments arranged by size:
2469 1802 734 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 2959 3848
Size: 54 1425 50 275 18 277 852 889
Cuts at: 3848 5477 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 852 277 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2959 1537
Size: 275 1147 4090
Fragments arranged by size:
4090 1147 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2540 3442 1353
Size: 1187 902 3423
Fragments arranged by size:
3423 1187 902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2200 4099 4473 4619 387
Size: 1064 138 611 1899 374 146 1280
Fragments arranged by size:
1899 1280 1064 611 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2172 3575 4172 4959 5227 1239
Size: 393 407 133 1403 597 787 268 1524
Fragments arranged by size:
1524 1403 787 597 407 393 268 133
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FspI TGC'GCA
Cuts at: 4018 4018
Size: 5512
HaeII r_GCGC'y
Cuts at: 1678 4659 1678
Size: 2981 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2508 2540 2594 2626 4153
Size: 32 135 32 936 32 54 32 1527
Cuts at: 4153 4185 4227 4259 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1527 936 135 54 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2856 3102 669
Size: 988 281 918 246 3079
Fragments arranged by size:
3079 988 918 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5512
HpaI GTT'AAC
Cuts at: 3102 3102
Size: 5512
KpnI G_GTAC'C
Cuts at: 2837 2837
Size: 5512
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 5512
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4626 4810 1305
Size: 3321 184 2007
Fragments arranged by size:
3321 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3690
Fragments arranged by size:
3690 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2560 3470 3829 3988 518
Size: 648 1394 910 359 159 2042
Fragments arranged by size:
2042 1394 910 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2127 2169 2928 3605 4753 4998 1606
Size: 405 116 42 759 677 1148 245 2120
Fragments arranged by size:
2120 1148 759 677 405 245 116 42
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4960
NdeI CA'TA_TG
Cuts at: 387 2750 387
Size: 2363 3149
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2959 2959
Size: 5512
NspI r_CATG'y
Cuts at: 1132 1229 2870 4415 1132
Size: 97 1641 1545 2229
Fragments arranged by size:
2229 1641 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5512
PciI A'CATG_T
Cuts at: 4411 4411
Size: 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5512
PfoI T'CCnGG_A
Cuts at: 2526 2526
Size: 5512
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5094 5126 3581
Size: 32 1481 32 3967
Fragments arranged by size:
3967 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5512
PsiI TTA'TAA
Cuts at: 3082 3082
Size: 5512
PstI C_TGCA'G
Cuts at: 830 2033 2422 2864 830
Size: 1203 389 442 3478
Fragments arranged by size:
3478 1203 442 389
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 3760 5472 3760
Size: 1712 3800
SfcI C'TryA_G
Cuts at: 826 1045 2029 2418 2860 3995 4676 4867 826
Size: 219 984 389 442 1135 681 191 1471
Fragments arranged by size:
1471 1135 984 681 442 389 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2839 1872
Size: 967 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3616 4517 4779 820
Size: 197 926 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5512
SphI G_CATG'C
Cuts at: 1132 2870 1132
Size: 1738 3774
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5512
StyI C'CwwG_G
Cuts at: 513 1065 2642 2951 513
Size: 552 1577 309 3074
Fragments arranged by size:
3074 1577 552 309
SwaI ATTT'AAAT
Cuts at: 2368 2368
Size: 5512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3539 3556 3709 3894 4339 325
Size: 1255 1959 17 153 185 445 1498
Fragments arranged by size:
1959 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3758 5470 96
Size: 274 80 33 51 1443 1781 1712 138
Fragments arranged by size:
1781 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5472 5504 5472
Size: 32 5480
Tth111I GACn'n_nGTC
Cuts at: 1316 2047 1316
Size: 731 4781
XbaI T'CTAG_A
Cuts at: 2848 2848
Size: 5512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2102 2102
Size: 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2837 1870
Size: 967 4545
XmnI GAAnn'nnTTC
Cuts at: 2637 3641 2637
Size: 1004 4508
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 276
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI