Restriction map for
pFN21AB8142
(Circular) (Six-base) MAPSORT of: pFN21AB8142.seq Check: 2348 from: 1 to: 5887
pFN21AB8142 5887 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2620 2620
Size: 5887
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2100 3230 1937
Size: 163 1130 4594
Fragments arranged by size:
4594 1130 163
Acc65I G'GTAC_C
Cuts at: 2511 2550 3208 2511
Size: 39 658 5190
Fragments arranged by size:
5190 658 39
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2549 3950 5334 5602 1632
Size: 917 1401 1384 268 1917
Fragments arranged by size:
1917 1401 1384 917 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 2952 4786 5763 1203
Size: 1749 1834 977 1327
Fragments arranged by size:
1834 1749 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AlwNI CAG_nnn'CTG
Cuts at: 2046 2527 2947 3630 5202 2046
Size: 481 420 683 1572 2731
Fragments arranged by size:
2731 1572 683 481 420
ApaI G_GGCC'C
Cuts at: 2897 3114 2897
Size: 217 5670
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 3196 3422 3574 5683 1493
Size: 1703 226 152 2109 1697
Fragments arranged by size:
2109 1703 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 3212 1870
Size: 73 1269 4545
Fragments arranged by size:
4545 1269 73
BamHI G'GATC_C
Cuts at: 3217 3584 3217
Size: 367 5520
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2488 2511 2550 3208 4663
Size: 325 217 627 701 23 39 658 1455
Cuts at: 4663 618
Size: 1842
Fragments arranged by size:
1842 1455 701 658 627 325 217 39
23
BanII G_rGCy'C
Cuts at: 1391 2298 2897 3114 3206 1391
Size: 907 599 217 92 4072
Fragments arranged by size:
4072 907 599 217 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 2809 928
Size: 971 189 721 4006
Fragments arranged by size:
4006 971 721 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4078 4112 1903
Size: 34 50 34 2057 34 3678
Fragments arranged by size:
3678 2057 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5882 5882
Size: 5887
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2491 2897 3114 3948 5104 1790
Size: 493 208 406 217 834 1156 2573
Fragments arranged by size:
2573 1156 834 493 406 217 208
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5887
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 3618 4576
Size: 416 53 83 399 24 897 1304 958
Cuts at: 4576 442
Size: 1753
Fragments arranged by size:
1753 1304 958 897 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2437 2762 3034 3126 4547 1239
Size: 1037 161 325 272 92 1421 2579
Fragments arranged by size:
2579 1421 1037 325 272 161 92
Bpu10I CC'TnA_GC
Cuts at: 2195 2195
Size: 5887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4012 4877 5175 4012
Size: 865 298 4724
Fragments arranged by size:
4724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4550 882
Size: 617 3051 2219
Fragments arranged by size:
3051 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2067 2953 3678 493
Size: 711 863 886 725 2702
Fragments arranged by size:
2702 886 863 725 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2365 3157 3694 4076
Size: 53 83 186 936 832 792 537 382
Cuts at: 4076 4711 275
Size: 635 1451
Fragments arranged by size:
1451 936 832 792 635 537 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2358 4320 4718 4992 5139 5550 2358
Size: 1962 398 274 147 411 2695
Fragments arranged by size:
2695 1962 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2423 2453 1178
Size: 30 165 30 1020 30 4612
Fragments arranged by size:
4612 1020 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3071 1186
Size: 1885 4002
BseYI C'CCAG_C
Cuts at: 2206 2298 2346 2385 2502 2563 5090 5586 2206
Size: 92 48 39 117 61 2527 496 2507
Fragments arranged by size:
2527 2507 496 117 92 61 48 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2933 1163
Size: 573 1197 4117
Fragments arranged by size:
4117 1197 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3948 4033 5104 3948
Size: 85 1071 4731
Fragments arranged by size:
4731 1071 85
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
BsmBI CGTCTCn'nnnn_
Cuts at: 2356 2356
Size: 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2298 2491 2897 3114 3206 3948
Size: 399 493 15 193 406 217 92 742
Cuts at: 3948 4033 5104 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 493 406 399 217 193
92 85 15
BspEI T'CCGG_A
Cuts at: 2358 5550 2358
Size: 3192 2695
BspHI T'CATG_A
Cuts at: 2994 3776 5506 2994
Size: 782 1730 3375
Fragments arranged by size:
3375 1730 782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2620 2694 2745 3242 844
Size: 1776 74 51 497 3489
Fragments arranged by size:
3489 1776 497 74 51
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4382 4556 65
Size: 1183 3134 174 1396
Fragments arranged by size:
3134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2199 4531 4718 1700
Size: 110 389 2332 187 2869
Fragments arranged by size:
2869 2332 389 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 2471 3947 4959 2471
Size: 1476 1012 3399
Fragments arranged by size:
3399 1476 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2947 794
Size: 2153 3734
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 2049 1184
Size: 865 5022
BstYI r'GATC_y
Cuts at: 1972 2853 3217 3584 3969 3986 5438 5524 5536
Size: 881 364 367 385 17 1452 86 12
Cuts at: 5536 5882 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 881 385 367 364 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5335
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2365 487
Size: 683 56 1139 4009
Fragments arranged by size:
4009 1139 683 56
BtsI GCAGTG_nn'
Cuts at: 2579 3403 4197 4217 2579
Size: 824 794 20 4249
Fragments arranged by size:
4249 824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5852
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DraIII CAC_nnn'GTG
Cuts at: 2647 2647
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4894 809
Size: 507 3578 1802
Fragments arranged by size:
3578 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2092 2154 2313
Size: 54 1425 50 275 18 262 62 159
Cuts at: 2313 3150 3334 4223 5852 8
Size: 837 184 889 1629 43
Fragments arranged by size:
1629 1425 889 837 275 262 184 159
62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2432 2801 3817 1353
Size: 1079 369 1016 3423
Fragments arranged by size:
3423 1079 1016 369
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4474 4848 4994 387
Size: 1064 138 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2437 2549 2762 3034 3126 3950
Size: 393 644 161 112 213 272 92 824
Cuts at: 3950 4547 5334 5602 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 824 787 644 597 393 272 268
213 161 112 92
EcoNI CCTnn'n_nnAGG
Cuts at: 2741 2741
Size: 5887
EcoO109I rG'GnC_Cy
Cuts at: 2893 2894 3110 2893
Size: 1 216 5670
Fragments arranged by size:
5670 216 1
EcoRI G'AATT_C
Cuts at: 3196 3574 3196
Size: 378 5509
FspI TGC'GCA
Cuts at: 2693 2978 4393 2693
Size: 285 1415 4187
Fragments arranged by size:
4187 1415 285
HaeII r_GCGC'y
Cuts at: 1678 2144 5034 1678
Size: 466 2890 2531
Fragments arranged by size:
2890 2531 466
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2823 2855 4528 4560 4602
Size: 32 135 32 1251 32 1673 32 42
Cuts at: 4602 4634 1373
Size: 32 2626
Fragments arranged by size:
2626 1673 1251 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2761 3231 3477 669
Size: 988 281 823 470 246 3079
Fragments arranged by size:
3079 988 823 470 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5887
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 2515 2554 3212 2515
Size: 39 658 5190
Fragments arranged by size:
5190 658 39
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5001 5185 1305
Size: 3696 184 2007
Fragments arranged by size:
3696 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2156 10
Size: 54 1425 343 324 3741
Fragments arranged by size:
3741 1425 343 324 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2482 2660 3845 4204 4363 518
Size: 648 1316 178 1185 359 159 2042
Fragments arranged by size:
2042 1316 1185 648 359 178 159
MspA1I CmG'CkG
Cuts at: 1606 2302 2389 2415 2944 3104 3303 3980 5128
Size: 696 87 26 529 160 199 677 1148
Cuts at: 5128 5373 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 696 677 529 245 199 160
87 26
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5335
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 2770 2983 3245 4790 1132
Size: 97 1541 213 262 1545 2229
Fragments arranged by size:
2229 1545 1541 262 213 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 1108
Size: 1539 4348
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PmlI CAC'GTG
Cuts at: 2067 2953 2067
Size: 886 5001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PspOMI G'GGCC_C
Cuts at: 2893 3110 2893
Size: 217 5670
PstI C_TGCA'G
Cuts at: 830 2340 2943 3087 3239 830
Size: 1510 603 144 152 3478
Fragments arranged by size:
3478 1510 603 152 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
PvuII CAG'CTG
Cuts at: 2302 2389 2415 2944 3104 2302
Size: 87 26 529 160 5085
Fragments arranged by size:
5085 529 160 87 26
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SfcI C'TryA_G
Cuts at: 826 1045 2336 2674 2939 2986 3083 3235 4370
Size: 219 1291 338 265 47 97 152 1135
Cuts at: 4370 5051 5242 826
Size: 681 191 1471
Fragments arranged by size:
1471 1291 1135 681 338 265 219 191
152 97 47
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3991 4892 5154 820
Size: 197 926 2048 901 262 1553
Fragments arranged by size:
2048 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 2983 3245 1132
Size: 1851 262 3774
Fragments arranged by size:
3774 1851 262
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 2747 1517
Size: 1230 4657
StyI C'CwwG_G
Cuts at: 513 1065 2289 3326 513
Size: 552 1224 1037 3074
Fragments arranged by size:
3074 1224 1037 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2071 2198 2449 2864 3914 3931 4084
Size: 1255 491 127 251 415 1050 17 153
Cuts at: 4084 4269 4714 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1050 491 445 415 251 185
153 127 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2541 4133 5845
Size: 274 80 33 51 1443 564 1592 1712
Cuts at: 5845 96
Size: 138
Fragments arranged by size:
1712 1592 1443 564 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5887
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2251 2499 2541 3058 2251
Size: 248 42 517 5080
Fragments arranged by size:
5080 517 248 42
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 2264 4016 2264
Size: 1752 4135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI