Restriction map for
pFN21AB8138
(Circular) (Six-base) MAPSORT of: pFN21AB8138.seq Check: 6939 from: 1 to: 6484
pFN21AB8138 6484 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2742 3473 2726
Size: 16 731 5737
Fragments arranged by size:
5737 731 16
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2899 3827 1937
Size: 962 928 4594
Fragments arranged by size:
4594 962 928
Acc65I G'GTAC_C
Cuts at: 3002 3710 3805 3002
Size: 708 95 5681
Fragments arranged by size:
5681 708 95
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4547 5931 6199 1632
Size: 2915 1384 268 1917
Fragments arranged by size:
2915 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 2984 3228 3230 5383 6360 1203
Size: 1781 244 2 2153 977 1327
Fragments arranged by size:
2153 1781 1327 977 244 2
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 2638 5213 2638
Size: 2575 3909
AleI CACnn'nnGTG
Cuts at: 1166 2319 3546 1166
Size: 1153 1227 4104
Fragments arranged by size:
4104 1227 1153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6484
AlwNI CAG_nnn'CTG
Cuts at: 2127 2253 3117 3185 3781 4227 5799 2127
Size: 126 864 68 596 446 1572 2812
Fragments arranged by size:
2812 1572 864 596 446 126 68
ApaI G_GGCC'C
Cuts at: 3021 3021
Size: 6484
ApaLI G'TGCA_C
Cuts at: 2489 4541 5697 2489
Size: 2052 1156 3276
Fragments arranged by size:
3276 2052 1156
ApoI r'AATT_y
Cuts at: 1493 3793 4019 4171 6280 1493
Size: 2300 226 152 2109 1697
Fragments arranged by size:
2300 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 3376 3809 1870
Size: 73 1433 433 4545
Fragments arranged by size:
4545 1433 433 73
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2061 2369 2507 2552 2646
Size: 325 217 627 274 308 138 45 94
Cuts at: 2646 3002 3028 3669 3710 3805 5260 618
Size: 356 26 641 41 95 1455 1842
Fragments arranged by size:
1842 1455 641 627 356 325 308 274
217 138 95 94 45 41 26
BanII G_rGCy'C
Cuts at: 1391 3021 3803 1391
Size: 1630 782 4072
Fragments arranged by size:
4072 1630 782
BbeI G_GCGC'C
Cuts at: 3673 3673
Size: 6484
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2487 928
Size: 971 588 4925
Fragments arranged by size:
4925 971 588
BbvCI CC'TCA_GC
Cuts at: 3059 3240 3059
Size: 181 6303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4675 4709 1903
Size: 34 2738 34 3678
Fragments arranged by size:
3678 2738 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2307 2880 3222
Size: 107 122 71 631 605 635 573 342
Cuts at: 3222 3302 3456 5095 136
Size: 80 154 1639 1525
Fragments arranged by size:
1639 1525 635 631 605 573 342 154
122 107 80 71
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 3901 3901
Size: 6484
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2384 2493 2555 2651 3021 3033 4545
Size: 276 318 109 62 96 370 12 1512
Cuts at: 4545 5701 1790
Size: 1156 2573
Fragments arranged by size:
2573 1512 1156 370 318 276 109 96
62 12
BmgBI CAC'GTC
Cuts at: 1318 3150 3559 1318
Size: 1832 409 4243
Fragments arranged by size:
4243 1832 409
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2673 4215 5173
Size: 416 53 83 399 24 1256 1542 958
Cuts at: 5173 442
Size: 1753
Fragments arranged by size:
1753 1542 1256 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3302 5144 1239
Size: 2063 1842 2579
Fragments arranged by size:
2579 2063 1842
Bpu10I CC'TnA_GC
Cuts at: 3059 3240 3059
Size: 181 6303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4609 5474 5772 3790
Size: 819 865 298 4502
Fragments arranged by size:
4502 865 819 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3508 5147 882
Size: 617 2009 1639 2219
Fragments arranged by size:
2219 2009 1639 617
BsaAI yAC'GTr
Cuts at: 493 1204 2418 2985 3231 3544 4275 493
Size: 711 1214 567 246 313 731 2702
Fragments arranged by size:
2702 1214 731 711 567 313 246
BsaBI GATnn'nnATC
Cuts at: 2289 6478 2289
Size: 4189 2295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3651 3670 3747 4291
Size: 53 83 186 936 2118 19 77 544
Cuts at: 4291 4673 5308 275
Size: 382 635 1451
Fragments arranged by size:
2118 1451 936 635 544 382 186 83
77 53 19
BsaWI w'CCGG_w
Cuts at: 2629 4917 5315 5589 5736 6147 2629
Size: 2288 398 274 147 411 2966
Fragments arranged by size:
2966 2288 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6259
Fragments arranged by size:
6259 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2124 2804 3115 1186
Size: 938 680 311 4555
Fragments arranged by size:
4555 938 680 311
BseYI C'CCAG_C
Cuts at: 2022 3049 3393 3510 3624 3741 3763 5687 6183
Size: 1027 344 117 114 117 22 1924 496
Cuts at: 6183 2022
Size: 2323
Fragments arranged by size:
2323 1924 1027 496 344 117 117 114
22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2343 2720 1163
Size: 573 607 377 4927
Fragments arranged by size:
4927 607 573 377
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3934 4695 5723 1540
Size: 275 186 1933 761 1028 2301
Fragments arranged by size:
2301 1933 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2493 4545 4630 5701 2493
Size: 2052 85 1071 3276
Fragments arranged by size:
3276 2052 1071 85
BsmI GAATG_Cn'
Cuts at: 2405 3995 4088 2405
Size: 1590 93 4801
Fragments arranged by size:
4801 1590 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2239 2239
Size: 6484
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2384 2493 2555 2651 3021 3033
Size: 399 276 318 109 62 96 370 12
Cuts at: 3033 3803 4545 4630 5701 1391
Size: 770 742 85 1071 2174
Fragments arranged by size:
2174 1071 770 742 399 370 318 276
109 96 85 62 12
BspEI T'CCGG_A
Cuts at: 2629 6147 2629
Size: 3518 2966
BspHI T'CATG_A
Cuts at: 4373 6103 4373
Size: 1730 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2672 2726 2742 2897 3143 3377 3473 3608
Size: 1828 54 16 155 246 234 96 135
Cuts at: 3608 3839 844
Size: 231 3489
Fragments arranged by size:
3489 1828 246 234 231 155 135 96
54 16
BsrBI CCG'CTC
Cuts at: 1394 1823 4371 1394
Size: 429 2548 3507
Fragments arranged by size:
3507 2548 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4979 5153 65
Size: 1183 3731 174 1396
Fragments arranged by size:
3731 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2210 5128 5315 1700
Size: 110 400 2918 187 2869
Fragments arranged by size:
2918 2869 400 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6484
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6484
BssSI C'ACGA_G
Cuts at: 4544 5556 4544
Size: 1012 5472
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6484
BstBI TT'CG_AA
Cuts at: 2397 5288 2397
Size: 2891 3593
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstXI CCAn_nnnn'nTGG
Cuts at: 3526 3526
Size: 6484
BstYI r'GATC_y
Cuts at: 1972 3814 4181 4566 4583 6035 6121 6133 6479
Size: 1842 367 385 17 1452 86 12 346
Cuts at: 6479 1972
Size: 1977
Fragments arranged by size:
1977 1842 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2271 2303 2364 2547 2805 2973 513
Size: 552 1206 32 61 183 258 168 4024
Fragments arranged by size:
4024 1206 552 258 183 168 61 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5745
Fragments arranged by size:
5745 683 56
BtsI GCAGTG_nn'
Cuts at: 2200 2293 2314 2326 2491 2607 2737 2962 3208
Size: 93 21 12 165 116 130 225 246
Cuts at: 3208 3254 3442 3673 4000 4794 4814 2200
Size: 46 188 231 327 794 20 3870
Fragments arranged by size:
3870 794 327 246 231 225 188 165
130 116 93 46 21 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3063 3098 529
Size: 35 2499 35 3915
Fragments arranged by size:
3915 2499 35 35
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DraIII CAC_nnn'GTG
Cuts at: 2760 2839 3028 2760
Size: 79 189 6216
Fragments arranged by size:
6216 189 79
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5491 809
Size: 507 4175 1802
Fragments arranged by size:
4175 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2212 2287 3931
Size: 54 1425 50 275 18 382 75 1644
Cuts at: 3931 4820 6449 8
Size: 889 1629 43
Fragments arranged by size:
1644 1629 1425 889 382 275 75 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3931 1537
Size: 275 2119 4090
Fragments arranged by size:
4090 2119 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3080 4414 1353
Size: 1727 1334 3423
Fragments arranged by size:
3423 1727 1334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5071 5445 5591 387
Size: 1064 138 3482 374 146 1280
Fragments arranged by size:
3482 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3302 4547 5144 5931 6199 1239
Size: 393 1670 1245 597 787 268 1524
Fragments arranged by size:
1670 1524 1245 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2789 2789
Size: 6484
EcoO109I rG'GnC_Cy
Cuts at: 2910 3017 3018 3046 3733 3760 2910
Size: 107 1 28 687 27 5634
Fragments arranged by size:
5634 687 107 28 27 1
EcoRI G'AATT_C
Cuts at: 3793 4171 3793
Size: 378 6106
FspI TGC'GCA
Cuts at: 2832 3700 3725 4990 2832
Size: 868 25 1265 4326
Fragments arranged by size:
4326 1265 868 25
HaeII r_GCGC'y
Cuts at: 1678 3673 5631 1678
Size: 1995 1958 2531
Fragments arranged by size:
2531 1995 1958
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2155 2187 2318 2350 2624
Size: 32 135 32 583 32 131 32 274
Cuts at: 2624 2656 5125 5157 5199 5231 1373
Size: 32 2469 32 42 32 2626
Fragments arranged by size:
2626 2469 583 274 135 131 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3828 4074 669
Size: 988 281 1890 246 3079
Fragments arranged by size:
3079 1890 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KasI G'GCGC_C
Cuts at: 3669 3669
Size: 6484
KpnI G_GTAC'C
Cuts at: 3006 3714 3809 3006
Size: 708 95 5681
Fragments arranged by size:
5681 708 95
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5598 5782 1305
Size: 4293 184 2007
Fragments arranged by size:
4293 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2289 10
Size: 54 1425 343 457 4205
Fragments arranged by size:
4205 1425 457 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 2319 2864 3546 4442 4801 4960
Size: 648 1009 144 545 682 896 359 159
Cuts at: 4960 518
Size: 2042
Fragments arranged by size:
2042 1009 896 682 648 545 359 159
144
MspA1I CmG'CkG
Cuts at: 1606 2124 2535 2975 3117 3351 3696 3778 3900
Size: 518 411 440 142 234 345 82 122
Cuts at: 3900 4577 5725 5970 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 518 440 411 345 245
234 142 122 82
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 6484
NarI GG'CG_CC
Cuts at: 3670 3670
Size: 6484
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5932
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 6484
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 2280 3842 5387 1132
Size: 97 1051 1562 1545 2229
Fragments arranged by size:
2229 1562 1545 1051 97
PasI CC'CwG_GG
Cuts at: 1348 3220 1348
Size: 1872 4612
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2563 1108
Size: 1455 5029
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PmlI CAC'GTG
Cuts at: 2418 3231 3544 2418
Size: 813 313 5358
Fragments arranged by size:
5358 813 313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3760 3760
Size: 6484
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PspOMI G'GGCC_C
Cuts at: 3017 3017
Size: 6484
PstI C_TGCA'G
Cuts at: 830 2321 3836 830
Size: 1491 1515 3478
Fragments arranged by size:
3478 1515 1491
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 2124 3117 3351 3778 2124
Size: 993 234 427 4830
Fragments arranged by size:
4830 993 427 234
SacII CC_GC'GG
Cuts at: 2976 2976
Size: 6484
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SanDI GG'GwC_CC
Cuts at: 3760 3760
Size: 6484
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SexAI A'CCwGG_T
Cuts at: 2520 3024 2520
Size: 504 5980
SfcI C'TryA_G
Cuts at: 826 1045 2119 2317 2900 3832 4967 5648 5839
Size: 219 1074 198 583 932 1135 681 191
Cuts at: 5839 826
Size: 1471
Fragments arranged by size:
1471 1135 1074 932 681 583 219 198
191
SfoI GGC'GCC
Cuts at: 3671 3671
Size: 6484
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3811 1872
Size: 1939 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3769 4588 5489 5751 820
Size: 197 926 1826 819 901 262 1553
Fragments arranged by size:
1826 1553 926 901 819 262 197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 2280 3842 1132
Size: 1148 1562 3774
Fragments arranged by size:
3774 1562 1148
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6484
StyI C'CwwG_G
Cuts at: 513 1065 2790 3923 513
Size: 552 1725 1133 3074
Fragments arranged by size:
3074 1725 1133 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2014 2165 2357 2560 2570 2608 3374
Size: 1255 434 151 192 203 10 38 766
Cuts at: 3374 3536 4511 4528 4681 4866 5311 325
Size: 162 975 17 153 185 445 1498
Fragments arranged by size:
1498 1255 975 766 445 434 203 192
185 162 153 151 38 17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4730 6442 96
Size: 274 80 33 51 1443 2753 1712 138
Fragments arranged by size:
2753 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2295 2327 3548 3580 6444 6476 2295
Size: 32 1221 32 2864 32 2303
Fragments arranged by size:
2864 2303 1221 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6484
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3149 3355 2011
Size: 1138 206 5140
Fragments arranged by size:
5140 1138 206
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 3809 1870
Size: 1939 4545
XmnI GAAnn'nnTTC
Cuts at: 4613 4613
Size: 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SapI
SfiI SrfI SwaI