Restriction map for
pFN21AB8137
(Circular) (Six-base) MAPSORT of: pFN21AB8137.seq Check: 1030 from: 1 to: 5740
pFN21AB8137 5740 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 2806 3061 2806
Size: 255 5485
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2633 3803 5187 5455 1632
Size: 1001 1170 1384 268 1917
Fragments arranged by size:
1917 1384 1170 1001 268
AfeI AGC'GCT
Cuts at: 2734 2734
Size: 5740
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2922 1184
Size: 1738 4002
AlwNI CAG_nnn'CTG
Cuts at: 2566 3483 5055 2566
Size: 917 1572 3251
Fragments arranged by size:
3251 1572 917
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 2174 2704 3049 3275 3427 5536 1493
Size: 681 530 345 226 152 2109 1697
Fragments arranged by size:
2109 1697 681 530 345 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 2512 2601 3065 1870
Size: 73 569 89 464 4545
Fragments arranged by size:
4545 569 464 89 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2356 2389 2356
Size: 33 5707
BamHI G'GATC_C
Cuts at: 3070 3437 3070
Size: 367 5373
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2806 3061 4516 618
Size: 325 217 627 1019 255 1455 1842
Fragments arranged by size:
1842 1455 1019 627 325 255 217
BanII G_rGCy'C
Cuts at: 1391 3059 1391
Size: 1668 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2621 2777 928
Size: 971 722 156 3891
Fragments arranged by size:
3891 971 722 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3931 3965 1903
Size: 34 1994 34 3678
Fragments arranged by size:
3678 1994 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BfrBI ATG'CAT
Cuts at: 2533 2533
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4351 136
Size: 107 122 71 631 605 2679 1525
Fragments arranged by size:
2679 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 3801 4957 1790
Size: 2011 1156 2573
Fragments arranged by size:
2573 2011 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3471 4429 442
Size: 416 53 83 399 24 2054 958 1753
Fragments arranged by size:
2054 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4400 1239
Size: 3161 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2982 3865 4730 5028 2982
Size: 883 865 298 3694
Fragments arranged by size:
3694 883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2996 4403 882
Size: 617 1497 1407 2219
Fragments arranged by size:
2219 1497 1407 617
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2160 2190 3547 3929
Size: 53 83 186 936 627 30 1357 382
Cuts at: 3929 4564 275
Size: 635 1451
Fragments arranged by size:
1451 1357 936 635 627 382 186 83
53 30
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5515
Fragments arranged by size:
5515 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2945 1186
Size: 1759 3981
BseYI C'CCAG_C
Cuts at: 2385 2735 2956 4943 5439 2385
Size: 350 221 1987 496 2686
Fragments arranged by size:
2686 1987 496 350 221
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3801 3886 4957 3801
Size: 85 1071 4584
Fragments arranged by size:
4584 1071 85
BsmI GAATG_Cn'
Cuts at: 3251 3344 3251
Size: 93 5647
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3059 3801 3886 4957 1391
Size: 399 1269 742 85 1071 2174
Fragments arranged by size:
2174 1269 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 2460 3629 5359 2460
Size: 1169 1730 2841
Fragments arranged by size:
2841 1730 1169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2627 3095 844
Size: 1783 468 3489
Fragments arranged by size:
3489 1783 468
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4235 4409 65
Size: 1183 2987 174 1396
Fragments arranged by size:
2987 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4384 4571 1700
Size: 110 2574 187 2869
Fragments arranged by size:
2869 2574 187 110
BsrGI T'GTAC_A
Cuts at: 96 2417 96
Size: 2321 3419
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 2293 2380 1184
Size: 1109 87 4544
Fragments arranged by size:
4544 1109 87
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2920 2356
Size: 564 5176
BstYI r'GATC_y
Cuts at: 1972 3070 3437 3822 3839 5291 5377 5389 5735
Size: 1098 367 385 17 1452 86 12 346
Cuts at: 5735 1972
Size: 1977
Fragments arranged by size:
1977 1452 1098 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2376 2972 513
Size: 552 1311 596 3281
Fragments arranged by size:
3281 1311 596 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 3256 4050 4070 3256
Size: 794 20 4926
Fragments arranged by size:
4926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4747 809
Size: 507 3431 1802
Fragments arranged by size:
3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2910 3187 4076
Size: 54 1425 50 275 18 1080 277 889
Cuts at: 4076 5705 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1080 889 277 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3670 1353
Size: 2317 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2643 4327 4701 4847 387
Size: 1064 138 1054 1684 374 146 1280
Fragments arranged by size:
1684 1280 1064 1054 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2633 3803 4400 5187 5455 1239
Size: 393 1001 1170 597 787 268 1524
Fragments arranged by size:
1524 1170 1001 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2250 2250
Size: 5740
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 5740
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 2736 4887 1678
Size: 1058 2151 2531
Fragments arranged by size:
2531 2151 1058
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2226 2258 2493 2525 2635
Size: 32 135 32 654 32 235 32 110
Cuts at: 2635 2667 2993 3025 4381 4413 4455 4487 1373
Size: 32 326 32 1356 32 42 32 2626
Fragments arranged by size:
2626 1356 654 326 235 135 110 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3084 3330 669
Size: 988 281 1146 246 3079
Fragments arranged by size:
3079 1146 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 2810 3065 2810
Size: 255 5485
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2748 4854 5038 1305
Size: 1443 2106 184 2007
Fragments arranged by size:
2106 2007 1443 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3918
Fragments arranged by size:
3918 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 2538 3698 4057 4216 518
Size: 648 1188 184 1160 359 159 2042
Fragments arranged by size:
2042 1188 1160 648 359 184 159
MspA1I CmG'CkG
Cuts at: 1606 2780 3156 3833 4981 5226 1606
Size: 1174 376 677 1148 245 2120
Fragments arranged by size:
2120 1174 1148 677 376 245
NcoI C'CATG_G
Cuts at: 513 1065 2376 513
Size: 552 1311 3877
Fragments arranged by size:
3877 1311 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NsiI A_TGCA'T
Cuts at: 2535 2535
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 3098 4643 1132
Size: 97 1869 1545 2229
Fragments arranged by size:
2229 1869 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2250 2250
Size: 5740
PshAI GACnn'nnGTC
Cuts at: 1613 2122 1613
Size: 509 5231
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PstI C_TGCA'G
Cuts at: 830 2124 3092 830
Size: 1294 968 3478
Fragments arranged by size:
3478 1294 968
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
PvuII CAG'CTG
Cuts at: 2780 2780
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 2120 3088 4223 4904 5095 826
Size: 219 1075 968 1135 681 191 1471
Fragments arranged by size:
1471 1135 1075 968 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2997 3844 4745 5007 820
Size: 197 926 1054 847 901 262 1553
Fragments arranged by size:
1553 1054 926 901 847 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 2547 3664 5
Size: 47 2495 1117 2081
Fragments arranged by size:
2495 2081 1117 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2376 2409 3179 513
Size: 552 1311 33 770 3074
Fragments arranged by size:
3074 1311 770 552 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2312 2399 3767 3784 3937 4122 4567
Size: 1255 732 87 1368 17 153 185 445
Cuts at: 4567 325
Size: 1498
Fragments arranged by size:
1498 1368 1255 732 445 185 153 87
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2417 3986 5698
Size: 274 80 33 51 1443 440 1569 1712
Cuts at: 5698 96
Size: 138
Fragments arranged by size:
1712 1569 1443 440 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2330 2362 5700 5732 2330
Size: 32 3338 32 2338
Fragments arranged by size:
3338 2338 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 3869 3869
Size: 5740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI