Restriction map for
pFN21AB8136
(Circular) (Six-base) MAPSORT of: pFN21AB8136.seq Check: 4454 from: 1 to: 6004
pFN21AB8136 6004 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3064 3064
Size: 6004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3347 1937
Size: 1410 4594
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2726 4067 5451 5719 1632
Size: 1094 1341 1384 268 1917
Fragments arranged by size:
1917 1384 1341 1094 268
AfeI AGC'GCT
Cuts at: 2827 2827
Size: 6004
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 2289 2805 3216 4903 5880 1203
Size: 1086 516 411 1687 977 1327
Fragments arranged by size:
1687 1327 1086 977 516 411
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2939 1184
Size: 1755 4249
AlwNI CAG_nnn'CTG
Cuts at: 3052 3124 3149 3747 5319 3052
Size: 72 25 598 1572 3737
Fragments arranged by size:
3737 1572 598 72 25
ApaI G_GGCC'C
Cuts at: 3093 3093
Size: 6004
ApaLI G'TGCA_C
Cuts at: 2469 2914 4061 5217 2469
Size: 445 1147 1156 3256
Fragments arranged by size:
3256 1156 1147 445
ApoI r'AATT_y
Cuts at: 1493 2172 2949 3313 3539 3691 5800 1493
Size: 679 777 364 226 152 2109 1697
Fragments arranged by size:
2109 1697 777 679 364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 2609 2691 3329 1870
Size: 73 339 327 82 638 4545
Fragments arranged by size:
4545 638 339 327 82 73
BamHI G'GATC_C
Cuts at: 3334 3701 3334
Size: 367 5637
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3325 4780 618
Size: 325 217 627 1538 1455 1842
Fragments arranged by size:
1842 1538 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3093 3323 1391
Size: 1702 230 4072
Fragments arranged by size:
4072 1702 230
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2901 3280 928
Size: 971 1002 379 3652
Fragments arranged by size:
3652 1002 971 379
BbvCI CC'TCA_GC
Cuts at: 2822 2822
Size: 6004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4195 4229 1903
Size: 34 2258 34 3678
Fragments arranged by size:
3678 2258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4615 136
Size: 107 122 71 631 605 2943 1525
Fragments arranged by size:
2943 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5999 5999
Size: 6004
BlpI GC'TnA_GC
Cuts at: 3080 3421 3080
Size: 341 5663
Bme1580I G_kGCm'C
Cuts at: 1790 2473 2757 2918 3093 4065 5221 1790
Size: 683 284 161 175 972 1156 2573
Fragments arranged by size:
2573 1156 972 683 284 175 161
BmgBI CAC'GTC
Cuts at: 1318 2434 2623 3166 1318
Size: 1116 189 543 4156
Fragments arranged by size:
4156 1116 543 189
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2457 3137 3194
Size: 416 53 83 399 24 1040 680 57
Cuts at: 3194 3735 4693 442
Size: 541 958 1753
Fragments arranged by size:
1753 1040 958 680 541 416 399 83
57 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3319 4664 1239
Size: 2080 1345 2579
Fragments arranged by size:
2579 2080 1345
Bpu10I CC'TnA_GC
Cuts at: 2822 2822
Size: 6004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4129 4994 5292 4129
Size: 865 298 4841
Fragments arranged by size:
4841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2081 2135 2957 4667 882
Size: 617 582 54 822 1710 2219
Fragments arranged by size:
2219 1710 822 617 582 54
BsaAI yAC'GTr
Cuts at: 493 1204 2266 2292 3795 493
Size: 711 1062 26 1503 2702
Fragments arranged by size:
2702 1503 1062 711 26
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4437 4835 5109 5256 5667 4437
Size: 398 274 147 411 4774
Fragments arranged by size:
4774 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3153 3183 3270 3300 1178
Size: 30 165 30 1750 30 87 30 3882
Fragments arranged by size:
3882 1750 165 87 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2178 1186
Size: 931 61 5012
Fragments arranged by size:
5012 931 61
BseYI C'CCAG_C
Cuts at: 2145 3234 5207 5703 2145
Size: 1089 1973 496 2446
Fragments arranged by size:
2446 1973 1089 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2083 2996 1163
Size: 573 347 913 4171
Fragments arranged by size:
4171 913 573 347
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2473 2793 2918 3081 4065 4150 5221 2473
Size: 320 125 163 984 85 1071 3256
Fragments arranged by size:
3256 1071 984 320 163 125 85
BsiWI C'GTAC_G
Cuts at: 2808 2808
Size: 6004
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
BsmBI CGTCTCn'nnnn_
Cuts at: 3156 3156
Size: 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2473 2757 2793 2918 3081 3093 3323
Size: 399 683 284 36 125 163 12 230
Cuts at: 3323 4065 4150 5221 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 399 284 230 163
125 85 36 12
BspEI T'CCGG_A
Cuts at: 5667 5667
Size: 6004
BspHI T'CATG_A
Cuts at: 2667 3129 3893 5623 2667
Size: 462 764 1730 3048
Fragments arranged by size:
3048 1730 764 462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2657 3064 3359 844
Size: 1813 407 295 3489
Fragments arranged by size:
3489 1813 407 295
BsrBI CCG'CTC
Cuts at: 1394 1823 2492 3891 1394
Size: 429 669 1399 3507
Fragments arranged by size:
3507 1399 669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4499 4673 65
Size: 1183 3251 174 1396
Fragments arranged by size:
3251 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4648 4835 1700
Size: 110 2838 187 2869
Fragments arranged by size:
2869 2838 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2477 2752 794
Size: 1683 275 4046
Fragments arranged by size:
4046 1683 275
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstXI CCAn_nnnn'nTGG
Cuts at: 2518 2518
Size: 6004
BstYI r'GATC_y
Cuts at: 1972 3334 3701 4086 4103 5555 5641 5653 5999
Size: 1362 367 385 17 1452 86 12 346
Cuts at: 5999 1972
Size: 1977
Fragments arranged by size:
1977 1452 1362 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2969 2969
Size: 6004
BtgI C'CryG_G
Cuts at: 513 1065 2004 2733 513
Size: 552 939 729 3784
Fragments arranged by size:
3784 939 729 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2101 487
Size: 683 56 875 4390
Fragments arranged by size:
4390 875 683 56
BtsI GCAGTG_nn'
Cuts at: 2071 2193 2767 3520 4314 4334 2071
Size: 122 574 753 794 20 3741
Fragments arranged by size:
3741 794 753 574 122 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2235 2270 529
Size: 35 1671 35 4263
Fragments arranged by size:
4263 1671 35 35
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DraIII CAC_nnn'GTG
Cuts at: 2752 2752
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3143 5011 809
Size: 507 1827 1868 1802
Fragments arranged by size:
1868 1827 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 3451 4340
Size: 54 1425 50 275 18 282 1339 889
Cuts at: 4340 5969 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1339 889 282 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2043 2298 2468 2798 3934 1353
Size: 690 255 170 330 1136 3423
Fragments arranged by size:
3423 1136 690 330 255 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2726 3319 4067 4664 5451 5719 1239
Size: 393 1094 593 748 597 787 268 1524
Fragments arranged by size:
1524 1094 787 748 597 593 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 3056 2084
Size: 972 5032
EcoO109I rG'GnC_Cy
Cuts at: 2646 3006 3090 2646
Size: 360 84 5560
Fragments arranged by size:
5560 360 84
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 6004
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 2829 5151 1678
Size: 1151 2322 2531
Fragments arranged by size:
2531 2322 1151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2443 2475 2888 2920 3153
Size: 32 135 32 871 32 413 32 233
Cuts at: 3153 3185 3248 3270 3280 3302 4645 4677 4719
Size: 32 63 22 10 22 1343 32 42
Cuts at: 4719 4751 1373
Size: 32 2626
Fragments arranged by size:
2626 1343 871 413 233 135 63 42
32 32 32 32 32 32 32 22
22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 2805 5880 2805
Size: 3075 2929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2910 3256 5118 5302 1305
Size: 1605 346 1862 184 2007
Fragments arranged by size:
2007 1862 1605 346 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2114 10
Size: 54 1425 343 282 3900
Fragments arranged by size:
3900 1425 343 282 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2268 2573 3221 3962 4321 4480 518
Size: 648 1102 305 648 741 359 159 2042
Fragments arranged by size:
2042 1102 741 648 648 359 305 159
MspA1I CmG'CkG
Cuts at: 1606 2539 3420 4097 5245 5490 1606
Size: 933 881 677 1148 245 2120
Fragments arranged by size:
2120 1148 933 881 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2733 513
Size: 552 939 729 3784
Fragments arranged by size:
3784 939 729 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 3220 3362 4907 1132
Size: 97 1991 142 1545 2229
Fragments arranged by size:
2229 1991 1545 142 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6004
PciI A'CATG_T
Cuts at: 3216 4903 3216
Size: 1687 4317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3111 1108
Size: 2003 4001
PfoI T'CCnGG_A
Cuts at: 2427 2427
Size: 6004
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PmlI CAC'GTG
Cuts at: 2266 2292 2266
Size: 26 5978
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2646 3006 2646
Size: 360 5644
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PspOMI G'GGCC_C
Cuts at: 3089 3089
Size: 6004
PstI C_TGCA'G
Cuts at: 830 2085 2200 2397 2550 2762 3057 3356 830
Size: 1255 115 197 153 212 295 299 3478
Fragments arranged by size:
3478 1255 299 295 212 197 153 115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 2539 2539
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SanDI GG'GwC_CC
Cuts at: 3006 3006
Size: 6004
SapI GCTCTTCn'nnn_
Cuts at: 2043 2798 2043
Size: 755 5249
SbfI CC_TGCA'GG
Cuts at: 2085 3057 3356 2085
Size: 972 299 4733
Fragments arranged by size:
4733 972 299
ScaI AGT'ACT
Cuts at: 2309 4252 5964 2309
Size: 1943 1712 2349
Fragments arranged by size:
2349 1943 1712
SfcI C'TryA_G
Cuts at: 826 1045 2081 2196 2393 2546 2758 3053 3352
Size: 219 1036 115 197 153 212 295 299
Cuts at: 3352 4487 5168 5359 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1036 681 299 295 219 212
197 191 153 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 2693 3331 1872
Size: 821 638 4545
Fragments arranged by size:
4545 821 638
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4108 5009 5271 820
Size: 197 926 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6004
StyI C'CwwG_G
Cuts at: 513 1065 2004 2733 3443 513
Size: 552 939 729 710 3074
Fragments arranged by size:
3074 939 729 710 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 4048 4201 4386 4831 325
Size: 1255 2451 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2092 2307 4250
Size: 274 80 33 51 1443 115 215 1943
Cuts at: 4250 5962 96
Size: 1712 138
Fragments arranged by size:
1943 1712 1443 274 215 138 115 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5964 5996 5964
Size: 32 5972
Tth111I GACn'n_nGTC
Cuts at: 1316 2717 3143 3167 1316
Size: 1401 426 24 4153
Fragments arranged by size:
4153 1401 426 24
XbaI T'CTAG_A
Cuts at: 2959 3340 2959
Size: 381 5623
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 2691 3329 1870
Size: 821 638 4545
Fragments arranged by size:
4545 821 638
XmnI GAAnn'nnTTC
Cuts at: 2172 4133 2172
Size: 1961 4043
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BstZ17I
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacI SacII
SexAI SfiI SfoI SrfI SwaI XcmI