Restriction map for
pFN21AB8135
(Circular) (Six-base) MAPSORT of: pFN21AB8135.seq Check: 2936 from: 1 to: 6196
pFN21AB8135 6196 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2910 4006 278
Size: 53 83 186 936 1374 1096 2468
Fragments arranged by size:
2468 1374 1096 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3539 1937
Size: 1602 4594
Acc65I G'GTAC_C
Cuts at: 3517 3517
Size: 6196
AclI AA'CG_TT
Cuts at: 1563 4323 4696 1563
Size: 2760 373 3063
Fragments arranged by size:
3063 2760 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 3230 4259 5643 5911 1632
Size: 557 1041 1029 1384 268 1917
Fragments arranged by size:
1917 1384 1041 1029 557 268
AflII C'TTAA_G
Cuts at: 820 1017 2668 820
Size: 197 1651 4348
Fragments arranged by size:
4348 1651 197
AflIII A'CryG_T
Cuts at: 1203 2179 5095 6072 1203
Size: 976 2916 977 1327
Fragments arranged by size:
2916 1327 977 976
AgeI A'CCGG_T
Cuts at: 5027 5027
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 2854 4925 2854
Size: 2071 4125
AleI CACnn'nnGTG
Cuts at: 1166 2150 1166
Size: 984 5212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6196
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2150 2182 2150
Size: 32 6164
AlwNI CAG_nnn'CTG
Cuts at: 3026 3208 3434 3939 5511 3026
Size: 182 226 505 1572 3711
Fragments arranged by size:
3711 1572 505 226 182
ApaLI G'TGCA_C
Cuts at: 3343 4253 5409 3343
Size: 910 1156 4130
Fragments arranged by size:
4130 1156 910
ApoI r'AATT_y
Cuts at: 1493 3505 3731 3883 5992 1493
Size: 2012 226 152 2109 1697
Fragments arranged by size:
2109 2012 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4750 160
Size: 872 3718 1606
Fragments arranged by size:
3718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 2018 2812 2830 3521 1870
Size: 73 75 794 18 691 4545
Fragments arranged by size:
4545 794 691 75 73 18
BamHI G'GATC_C
Cuts at: 3526 3893 3526
Size: 367 5829
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2418 3109 3517 4972
Size: 325 217 627 249 382 691 408 1455
Cuts at: 4972 618
Size: 1842
Fragments arranged by size:
1842 1455 691 627 408 382 325 249
217
BanII G_rGCy'C
Cuts at: 1391 2019 3515 1391
Size: 628 1496 4072
Fragments arranged by size:
4072 1496 628
BbeI G_GCGC'C
Cuts at: 2040 2040
Size: 6196
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2131 2902 3218 928
Size: 971 232 771 316 3906
Fragments arranged by size:
3906 971 771 316 232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4387 4421 1903
Size: 34 2450 34 3678
Fragments arranged by size:
3678 2450 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4088 5298 4088
Size: 1210 4986
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6196
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2786 4807
Size: 107 122 71 631 605 337 777 2021
Cuts at: 4807 136
Size: 1525
Fragments arranged by size:
2021 1525 777 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6191 6191
Size: 6196
BlpI GC'TnA_GC
Cuts at: 3613 3613
Size: 6196
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2839 3347 4257 5413 1790
Size: 235 814 508 910 1156 2573
Fragments arranged by size:
2573 1156 910 814 508 235
BmgBI CAC'GTC
Cuts at: 1318 2148 1318
Size: 830 5366
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2389 2672 3927
Size: 416 53 83 399 24 972 283 1255
Cuts at: 3927 4885 442
Size: 958 1753
Fragments arranged by size:
1753 1255 972 958 416 399 283 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3155 4856 1239
Size: 1916 1701 2579
Fragments arranged by size:
2579 1916 1701
Bpu10I CC'TnA_GC
Cuts at: 2461 2970 2461
Size: 509 5687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4321 5186 5484 4321
Size: 865 298 5033
Fragments arranged by size:
5033 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2270 4859 882
Size: 617 771 2589 2219
Fragments arranged by size:
2589 2219 771 617
BsaAI yAC'GTr
Cuts at: 493 1204 3463 3987 493
Size: 711 2259 524 2702
Fragments arranged by size:
2702 2259 711 524
BsaBI GATnn'nnATC
Cuts at: 6190 6190
Size: 6196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 2907 4003 4385
Size: 53 83 186 936 504 870 1096 382
Cuts at: 4385 5020 275
Size: 635 1451
Fragments arranged by size:
1451 1096 936 870 635 504 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4629 5027 5301 5448 5859 4629
Size: 398 274 147 411 4966
Fragments arranged by size:
4966 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2486 2516 3356 3386 1178
Size: 30 165 30 1083 30 840 30 3988
Fragments arranged by size:
3988 1083 840 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2319 1186
Size: 1133 5063
BseYI C'CCAG_C
Cuts at: 5399 5895 5399
Size: 496 5700
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2963 1163
Size: 573 1227 4396
Fragments arranged by size:
4396 1227 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2470 3325 3646 4407 5435 1540
Size: 275 186 469 855 321 761 1028 2301
Fragments arranged by size:
2301 1028 855 761 469 321 275 186
BsiHKAI G_wGCw'C
Cuts at: 3347 4257 4342 5413 3347
Size: 910 85 1071 4130
Fragments arranged by size:
4130 1071 910 85
BsiWI C'GTAC_G
Cuts at: 2413 2413
Size: 6196
BsmI GAATG_Cn'
Cuts at: 2565 3707 3800 2565
Size: 1142 93 4961
Fragments arranged by size:
4961 1142 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2025 2839 3347 3515 4257 4342
Size: 399 229 6 814 508 168 742 85
Cuts at: 4342 5413 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 814 742 508 399 229 168
85 6
BspEI T'CCGG_A
Cuts at: 5859 5859
Size: 6196
BspHI T'CATG_A
Cuts at: 4085 5815 4085
Size: 1730 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3551 844
Size: 2707 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3295 4083 1394
Size: 429 1472 788 3507
Fragments arranged by size:
3507 1472 788 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2768 4691 4865 65
Size: 1183 1520 1923 174 1396
Fragments arranged by size:
1923 1520 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3347 4840 5027 1700
Size: 110 1537 1493 187 2869
Fragments arranged by size:
2869 1537 1493 187 110
BsrGI T'GTAC_A
Cuts at: 96 2442 96
Size: 2346 3850
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 4256 5268 4256
Size: 1012 5184
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3351 794
Size: 2557 3639
BstBI TT'CG_AA
Cuts at: 5000 5000
Size: 6196
BstEII G'GTnAC_C
Cuts at: 1184 2092 3487 1184
Size: 908 1395 3893
Fragments arranged by size:
3893 1395 908
BstXI CCAn_nnnn'nTGG
Cuts at: 2152 2261 2152
Size: 109 6087
BstYI r'GATC_y
Cuts at: 1972 2610 3276 3526 3893 4278 4295 5747 5833
Size: 638 666 250 367 385 17 1452 86
Cuts at: 5833 5845 6191 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 666 638 385 367 346 250
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2526 2784 2859 3149 3492 513
Size: 552 939 522 258 75 290 343 3217
Fragments arranged by size:
3217 939 552 522 343 290 258 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5457
Fragments arranged by size:
5457 683 56
BtsI GCAGTG_nn'
Cuts at: 2597 2871 3712 4506 4526 2597
Size: 274 841 794 20 4267
Fragments arranged by size:
4267 841 794 274 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2500 2535 529
Size: 35 1936 35 4190
Fragments arranged by size:
4190 1936 35 35
DraI TTT'AAA
Cuts at: 3500 3500
Size: 6196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5203 809
Size: 507 3887 1802
Fragments arranged by size:
3887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2350 2467
Size: 54 1425 50 275 18 279 241 117
Cuts at: 2467 3322 3421 3643 4532 6161 8
Size: 855 99 222 889 1629 43
Fragments arranged by size:
1629 1425 889 855 279 275 241 222
117 99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2467 3322 3643 1537
Size: 275 655 855 321 4090
Fragments arranged by size:
4090 855 655 321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2530 3165 4126 1353
Size: 1177 635 961 3423
Fragments arranged by size:
3423 1177 961 635
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 4783 5157 5303 387
Size: 1064 138 438 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 3155 3230 4259 4856 5643 5911
Size: 393 557 966 75 1029 597 787 268
Cuts at: 5911 1239
Size: 1524
Fragments arranged by size:
1524 1029 966 787 597 557 393 268
75
EcoNI CCTnn'n_nnAGG
Cuts at: 2600 2600
Size: 6196
EcoO109I rG'GnC_Cy
Cuts at: 2044 2318 2383 2044
Size: 274 65 5857
Fragments arranged by size:
5857 274 65
EcoRI G'AATT_C
Cuts at: 3505 3883 3505
Size: 378 5818
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 2040 5343 1678
Size: 362 3303 2531
Fragments arranged by size:
3303 2531 362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2466 2498 2840 2872 4837
Size: 32 135 32 894 32 342 32 1965
Cuts at: 4837 4869 4911 4943 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1965 894 342 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 3181 3316 3364 3540 3786
Size: 988 281 1122 121 135 48 176 246
Cuts at: 3786 669
Size: 3079
Fragments arranged by size:
3079 1122 988 281 246 176 135 121
48
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6196
HpaI GTT'AAC
Cuts at: 3316 3786 3316
Size: 470 5726
KasI G'GCGC_C
Cuts at: 2036 2036
Size: 6196
KpnI G_GTAC'C
Cuts at: 3521 3521
Size: 6196
MfeI C'AATT_G
Cuts at: 3795 3795
Size: 6196
MluI A'CGCG_T
Cuts at: 6072 6072
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2357 5310 5494 1305
Size: 1052 2953 184 2007
Fragments arranged by size:
2953 2007 1052 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2111 10
Size: 54 1425 343 279 4095
Fragments arranged by size:
4095 1425 343 279 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2030 2150 2259 4154 4513 4672 518
Size: 648 864 120 109 1895 359 159 2042
Fragments arranged by size:
2042 1895 864 648 359 159 120 109
MspA1I CmG'CkG
Cuts at: 1606 2072 2433 2786 3000 3151 3208 3434 3612
Size: 466 361 353 214 151 57 226 178
Cuts at: 3612 4289 5437 5682 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 466 361 353 245 226
214 178 151 57
NarI GG'CG_CC
Cuts at: 2037 2037
Size: 6196
NcoI C'CATG_G
Cuts at: 513 1065 2004 2526 513
Size: 552 939 522 4183
Fragments arranged by size:
4183 939 552 522
NdeI CA'TA_TG
Cuts at: 387 3416 387
Size: 3029 3167
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6196
NotI GC'GGCC_GC
Cuts at: 3643 3643
Size: 6196
NspI r_CATG'y
Cuts at: 1132 1229 2183 3239 3554 5099 1132
Size: 97 954 1056 315 1545 2229
Fragments arranged by size:
2229 1545 1056 954 315 97
PasI CC'CwG_GG
Cuts at: 1348 2899 1348
Size: 1551 4645
PciI A'CATG_T
Cuts at: 2179 5095 2179
Size: 2916 3280
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6196
PfoI T'CCnGG_A
Cuts at: 2601 2601
Size: 6196
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PmlI CAC'GTG
Cuts at: 3463 3463
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 5778 5810 4265
Size: 32 1481 32 4651
Fragments arranged by size:
4651 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2318 2383 2318
Size: 65 6131
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6196
PsiI TTA'TAA
Cuts at: 3766 3766
Size: 6196
PstI C_TGCA'G
Cuts at: 830 2131 3548 830
Size: 1301 1417 3478
Fragments arranged by size:
3478 1417 1301
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
PvuII CAG'CTG
Cuts at: 3000 3208 3434 3000
Size: 208 226 5762
Fragments arranged by size:
5762 226 208
SacII CC_GC'GG
Cuts at: 2787 3152 2787
Size: 365 5831
SalI G'TCGA_C
Cuts at: 1936 3538 1936
Size: 1602 4594
SanDI GG'GwC_CC
Cuts at: 2383 2383
Size: 6196
SapI GCTCTTCn'nnn_
Cuts at: 2530 2530
Size: 6196
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 4444 6156 4444
Size: 1712 4484
SfcI C'TryA_G
Cuts at: 826 1045 2127 3544 4679 5360 5551 826
Size: 219 1082 1417 1135 681 191 1471
Fragments arranged by size:
1471 1417 1135 1082 681 219 191
SfoI GGC'GCC
Cuts at: 2038 2038
Size: 6196
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6196
SmaI CCC'GGG
Cuts at: 1872 2020 3523 1872
Size: 148 1503 4545
Fragments arranged by size:
4545 1503 148
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2668 4300 5201 5463 820
Size: 197 926 725 1632 901 262 1553
Fragments arranged by size:
1632 1553 926 901 725 262 197
SnaBI TAC'GTA
Cuts at: 493 3987 493
Size: 3494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 3239 3554 1132
Size: 2107 315 3774
Fragments arranged by size:
3774 2107 315
SrfI GCCC'GGGC
Cuts at: 2020 2020
Size: 6196
SspI AAT'ATT
Cuts at: 5 52 4120 5
Size: 47 4068 2081
Fragments arranged by size:
4068 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6196
StyI C'CwwG_G
Cuts at: 513 1065 2004 2526 3635 513
Size: 552 939 522 1109 3074
Fragments arranged by size:
3074 1109 939 552 522
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2865 4223 4240 4393 4578 5023 325
Size: 1255 1285 1358 17 153 185 445 1498
Fragments arranged by size:
1498 1358 1285 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2442 4442 6154
Size: 274 80 33 51 1443 465 2000 1712
Cuts at: 6154 96
Size: 138
Fragments arranged by size:
2000 1712 1443 465 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2302 2334 2758 2790 6156 6188 2302
Size: 32 424 32 3366 32 2310
Fragments arranged by size:
3366 2310 424 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2135 3455 1316
Size: 819 1320 4057
Fragments arranged by size:
4057 1320 819
XbaI T'CTAG_A
Cuts at: 3532 3532
Size: 6196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2104 2360 2104
Size: 256 5940
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 2018 3521 1870
Size: 148 1503 4545
Fragments arranged by size:
4545 1503 148
XmnI GAAnn'nnTTC
Cuts at: 4325 4325
Size: 6196
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2908 4004 276
Size: 53 83 186 936 1374 1096 2468
Fragments arranged by size:
2468 1374 1096 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacI SexAI SfiI SwaI