Restriction map for
pFN21AB8134
(Circular) (Six-base) MAPSORT of: pFN21AB8134.seq Check: 9081 from: 1 to: 5551
pFN21AB8134 5551 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2171 2171
Size: 5551
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3361 278
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2079 2894 1937
Size: 142 815 4594
Fragments arranged by size:
4594 815 142
Acc65I G'GTAC_C
Cuts at: 2676 2872 2676
Size: 196 5355
AclI AA'CG_TT
Cuts at: 1563 3678 4051 1563
Size: 2115 373 3063
Fragments arranged by size:
3063 2115 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2774 3614 4998 5266 1632
Size: 1142 840 1384 268 1917
Fragments arranged by size:
1917 1384 1142 840 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5354
AflIII A'CryG_T
Cuts at: 1203 4450 5427 1203
Size: 3247 977 1327
Fragments arranged by size:
3247 1327 977
AgeI A'CCGG_T
Cuts at: 4382 4382
Size: 5551
AhdI GACnn_n'nnGTC
Cuts at: 4280 4280
Size: 5551
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5551
AlwNI CAG_nnn'CTG
Cuts at: 2731 3294 4866 2731
Size: 563 1572 3416
Fragments arranged by size:
3416 1572 563
ApaI G_GGCC'C
Cuts at: 2047 2047
Size: 5551
ApaLI G'TGCA_C
Cuts at: 3608 4764 3608
Size: 1156 4395
ApoI r'AATT_y
Cuts at: 1493 2860 3086 3238 5347 1493
Size: 1367 226 152 2109 1697
Fragments arranged by size:
2109 1697 1367 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4105 160
Size: 872 3073 1606
Fragments arranged by size:
3073 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
AvaI C'yCGr_G
Cuts at: 1870 1943 2622 2876 1870
Size: 73 679 254 4545
Fragments arranged by size:
4545 679 254 73
BamHI G'GATC_C
Cuts at: 2881 3248 2881
Size: 367 5184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2261 2676 2872 4327 618
Size: 325 217 627 474 415 196 1455 1842
Fragments arranged by size:
1842 1455 627 474 415 325 217 196
BanII G_rGCy'C
Cuts at: 1391 2047 2143 2175 2870 1391
Size: 656 96 32 695 4072
Fragments arranged by size:
4072 695 656 96 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2349 928
Size: 971 450 4130
Fragments arranged by size:
4130 971 450
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3742 3776 1903
Size: 34 1805 34 3678
Fragments arranged by size:
3678 1805 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2467 3443 4653 2467
Size: 976 1210 3365
Fragments arranged by size:
3365 1210 976
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2114 4162 136
Size: 107 122 71 631 605 442 2048 1525
Fragments arranged by size:
2048 1525 631 605 442 122 107 71
BglII A'GATC_T
Cuts at: 5546 5546
Size: 5551
BlpI GC'TnA_GC
Cuts at: 2968 2968
Size: 5551
Bme1580I G_kGCm'C
Cuts at: 1790 2047 3612 4768 1790
Size: 257 1565 1156 2573
Fragments arranged by size:
2573 1565 1156 257
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5551
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3282 4240 442
Size: 416 53 83 399 24 1865 958 1753
Fragments arranged by size:
1865 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2165 2261 2732 2753 4211 1239
Size: 812 114 96 471 21 1458 2579
Fragments arranged by size:
2579 1458 812 471 114 96 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2303 3676 4541 4839 2303
Size: 1373 865 298 3015
Fragments arranged by size:
3015 1373 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2643 4214 882
Size: 617 1144 1571 2219
Fragments arranged by size:
2219 1571 1144 617
BsaAI yAC'GTr
Cuts at: 493 1204 3342 493
Size: 711 2138 2702
Fragments arranged by size:
2702 2138 711
BsaBI GATnn'nnATC
Cuts at: 5545 5545
Size: 5551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3358 3740 4375 275
Size: 53 83 186 936 1825 382 635 1451
Fragments arranged by size:
1825 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3984 4382 4656 4803 5214 3984
Size: 398 274 147 411 4321
Fragments arranged by size:
4321 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2705 2735 1178
Size: 30 165 30 1302 30 3994
Fragments arranged by size:
3994 1302 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 2306 2556 1186
Size: 1036 84 250 4181
Fragments arranged by size:
4181 1036 250 84
BseYI C'CCAG_C
Cuts at: 2053 2065 2457 4754 5250 2053
Size: 12 392 2297 496 2354
Fragments arranged by size:
2354 2297 496 392 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4978
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3001 3762 4790 1540
Size: 275 186 1000 761 1028 2301
Fragments arranged by size:
2301 1028 1000 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3612 3697 4768 3612
Size: 85 1071 4395
Fragments arranged by size:
4395 1071 85
BsmI GAATG_Cn'
Cuts at: 3062 3155 3062
Size: 93 5458
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 2143 2175 2870 3612 3697 4768
Size: 399 257 96 32 695 742 85 1071
Cuts at: 4768 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 695 399 257 96 85
32
BspEI T'CCGG_A
Cuts at: 5214 5214
Size: 5551
BspHI T'CATG_A
Cuts at: 3440 5170 3440
Size: 1730 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2171 2811 2906 844
Size: 1327 640 95 3489
Fragments arranged by size:
3489 1327 640 95
BsrBI CCG'CTC
Cuts at: 1394 1823 3438 1394
Size: 429 1615 3507
Fragments arranged by size:
3507 1615 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4046 4220 65
Size: 1183 2798 174 1396
Fragments arranged by size:
2798 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4195 4382 1700
Size: 110 2385 187 2869
Fragments arranged by size:
2869 2385 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5551
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5551
BssSI C'ACGA_G
Cuts at: 3611 4623 3611
Size: 1012 4539
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5551
BstBI TT'CG_AA
Cuts at: 4355 4355
Size: 5551
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5551
BstYI r'GATC_y
Cuts at: 1972 2784 2881 3248 3633 3650 5102 5188 5200
Size: 812 97 367 385 17 1452 86 12
Cuts at: 5200 5546 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 812 385 367 346 97 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2772 513
Size: 552 1707 3292
Fragments arranged by size:
3292 1707 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4812
Fragments arranged by size:
4812 683 56
BtsI GCAGTG_nn'
Cuts at: 3067 3861 3881 3067
Size: 794 20 4737
Fragments arranged by size:
4737 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5516
DraI TTT'AAA
Cuts at: 2855 2855
Size: 5551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4558 809
Size: 507 3242 1802
Fragments arranged by size:
3242 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2490 2998 3887
Size: 54 1425 50 275 18 660 508 889
Cuts at: 3887 5516 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 660 508 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2998 1537
Size: 275 1186 4090
Fragments arranged by size:
4090 1186 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2639 3481 1353
Size: 1286 842 3423
Fragments arranged by size:
3423 1286 842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4138 4512 4658 387
Size: 1064 138 2549 374 146 1280
Fragments arranged by size:
2549 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2165 2261 2732 2753 2774 3614
Size: 393 419 114 96 471 21 21 840
Cuts at: 3614 4211 4998 5266 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 840 787 597 471 419 393 268
114 96 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2321 2321
Size: 5551
EcoO109I rG'GnC_Cy
Cuts at: 2043 2044 2128 2707 2800 2838 2043
Size: 1 84 579 93 38 4756
Fragments arranged by size:
4756 579 93 84 38 1
EcoRI G'AATT_C
Cuts at: 2860 3238 2860
Size: 378 5173
FspI TGC'GCA
Cuts at: 4057 4057
Size: 5551
HaeII r_GCGC'y
Cuts at: 1678 4698 1678
Size: 3020 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 2524 2556 4192
Size: 32 135 32 605 32 315 32 1636
Cuts at: 4192 4224 4266 4298 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1636 605 315 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2895 3141 669
Size: 988 281 957 246 3079
Fragments arranged by size:
3079 988 957 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5551
HpaI GTT'AAC
Cuts at: 3141 3141
Size: 5551
KpnI G_GTAC'C
Cuts at: 2680 2876 2680
Size: 196 5355
MfeI C'AATT_G
Cuts at: 3150 3150
Size: 5551
MluI A'CGCG_T
Cuts at: 5427 5427
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4665 4849 1305
Size: 3360 184 2007
Fragments arranged by size:
3360 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 10
Size: 54 1425 343 660 3069
Fragments arranged by size:
3069 1425 660 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3509 3868 4027 518
Size: 648 2343 359 159 2042
Fragments arranged by size:
2343 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2686 2967 3644 4792 5037 1606
Size: 1080 281 677 1148 245 2120
Fragments arranged by size:
2120 1148 1080 677 281 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4999
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5551
NotI GC'GGCC_GC
Cuts at: 2998 2998
Size: 5551
NruI TCG'CGA
Cuts at: 2748 2748
Size: 5551
NspI r_CATG'y
Cuts at: 1132 1229 2909 4454 1132
Size: 97 1680 1545 2229
Fragments arranged by size:
2229 1680 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2023 1348
Size: 675 4876
PciI A'CATG_T
Cuts at: 4450 4450
Size: 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2060 2731 1108
Size: 952 671 3928
Fragments arranged by size:
3928 952 671
PmeI GTTT'AAAC
Cuts at: 2855 2855
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 5133 5165 3620
Size: 32 1481 32 4006
Fragments arranged by size:
4006 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2128 2800 2838 2128
Size: 672 38 4841
Fragments arranged by size:
4841 672 38
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5551
PsiI TTA'TAA
Cuts at: 3121 3121
Size: 5551
PspOMI G'GGCC_C
Cuts at: 2043 2043
Size: 5551
PstI C_TGCA'G
Cuts at: 830 2903 830
Size: 2073 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5551
SalI G'TCGA_C
Cuts at: 1936 2893 1936
Size: 957 4594
SbfI CC_TGCA'GG
Cuts at: 2903 2903
Size: 5551
ScaI AGT'ACT
Cuts at: 3799 5511 3799
Size: 1712 3839
SfcI C'TryA_G
Cuts at: 826 1045 2899 4034 4715 4906 826
Size: 219 1854 1135 681 191 1471
Fragments arranged by size:
1854 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5551
SmaI CCC'GGG
Cuts at: 1872 2878 1872
Size: 1006 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2318 3655 4556 4818 820
Size: 197 926 375 1337 901 262 1553
Fragments arranged by size:
1553 1337 926 901 375 262 197
SnaBI TAC'GTA
Cuts at: 493 3342 493
Size: 2849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5551
SphI G_CATG'C
Cuts at: 1132 2909 1132
Size: 1777 3774
SspI AAT'ATT
Cuts at: 5 52 3475 5
Size: 47 3423 2081
Fragments arranged by size:
3423 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5551
StyI C'CwwG_G
Cuts at: 513 1065 2131 2990 513
Size: 552 1066 859 3074
Fragments arranged by size:
3074 1066 859 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3578 3595 3748 3933 4378 325
Size: 1255 1998 17 153 185 445 1498
Fragments arranged by size:
1998 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3797 5509 96
Size: 274 80 33 51 1443 1820 1712 138
Fragments arranged by size:
1820 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2705 2737 5511 5543 2705
Size: 32 2774 32 2713
Fragments arranged by size:
2774 2713 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5551
XbaI T'CTAG_A
Cuts at: 2887 2887
Size: 5551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5551
XmaI C'CCGG_G
Cuts at: 1870 2876 1870
Size: 1006 4545
XmnI GAAnn'nnTTC
Cuts at: 3680 3680
Size: 5551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3359 276
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI