Restriction map for
pFN21AB8133
(Circular) (Six-base) MAPSORT of: pFN21AB8133.seq Check: 8222 from: 1 to: 6403
pFN21AB8133 6403 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3441 4213 278
Size: 53 83 186 936 1905 772 2468
Fragments arranged by size:
2468 1905 936 772 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2046 3746 1937
Size: 109 1700 4594
Fragments arranged by size:
4594 1700 109
Acc65I G'GTAC_C
Cuts at: 2316 3249 3724 2316
Size: 933 475 4995
Fragments arranged by size:
4995 933 475
AclI AA'CG_TT
Cuts at: 1563 4530 4903 1563
Size: 2967 373 3063
Fragments arranged by size:
3063 2967 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3541 4466 5850 6118 1632
Size: 1909 925 1384 268 1917
Fragments arranged by size:
1917 1909 1384 925 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6206
AflIII A'CryG_T
Cuts at: 1203 2574 5302 6279 1203
Size: 1371 2728 977 1327
Fragments arranged by size:
2728 1371 1327 977
AgeI A'CCGG_T
Cuts at: 5234 5234
Size: 6403
AhdI GACnn_n'nnGTC
Cuts at: 5132 5132
Size: 6403
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2042 1184
Size: 858 5545
AlwNI CAG_nnn'CTG
Cuts at: 2230 2266 2290 3115 3577 4146 5718 2230
Size: 36 24 825 462 569 1572 2915
Fragments arranged by size:
2915 1572 825 569 462 36 24
ApaI G_GGCC'C
Cuts at: 2072 3144 2072
Size: 1072 5331
ApaLI G'TGCA_C
Cuts at: 4460 5616 4460
Size: 1156 5247
ApoI r'AATT_y
Cuts at: 1493 3712 3938 4090 6199 1493
Size: 2219 226 152 2109 1697
Fragments arranged by size:
2219 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4957 160
Size: 872 3925 1606
Fragments arranged by size:
3925 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6403
AvaI C'yCGr_G
Cuts at: 1870 1943 2470 2611 3020 3728 1870
Size: 73 527 141 409 708 4545
Fragments arranged by size:
4545 708 527 409 141 73
BamHI G'GATC_C
Cuts at: 3733 4100 3733
Size: 367 6036
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2242 2316 3038 3249 3649
Size: 325 217 627 455 74 722 211 400
Cuts at: 3649 3724 5179 618
Size: 75 1455 1842
Fragments arranged by size:
1842 1455 722 627 455 400 325 217
211 75 74
BanII G_rGCy'C
Cuts at: 1391 2072 3144 3167 3382 3722 1391
Size: 681 1072 23 215 340 4072
Fragments arranged by size:
4072 1072 681 340 215 23
BbeI G_GCGC'C
Cuts at: 3042 3042
Size: 6403
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3547 928
Size: 971 1648 3784
Fragments arranged by size:
3784 1648 971
BbvCI CC'TCA_GC
Cuts at: 2297 2297
Size: 6403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2764 2798 3011 3045 3433 3467 4594
Size: 34 827 34 213 34 388 34 1127
Cuts at: 4594 4628 1903
Size: 34 3678
Fragments arranged by size:
3678 1127 827 388 213 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4295 5505 4295
Size: 1210 5193
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6403
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3464 5014 136
Size: 107 122 71 631 605 1792 1550 1525
Fragments arranged by size:
1792 1550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2052 2355 6398 2052
Size: 303 4043 2057
Fragments arranged by size:
4043 2057 303
BlpI GC'TnA_GC
Cuts at: 3820 3820
Size: 6403
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2187 2247 3144 4464 5620 1790
Size: 282 115 60 897 1320 1156 2573
Fragments arranged by size:
2573 1320 1156 897 282 115 60
BmgBI CAC'GTC
Cuts at: 1318 2080 2182 2575 2826 3196 1318
Size: 762 102 393 251 370 4525
Fragments arranged by size:
4525 762 393 370 251 102
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2422 2431 4134
Size: 416 53 83 399 24 1005 9 1703
Cuts at: 4134 5092 442
Size: 958 1753
Fragments arranged by size:
1753 1703 1005 958 416 399 83 53
24 9
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6403
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3396 3396
Size: 6403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2380 2437 2954 3629 5063 1239
Size: 1141 57 517 675 1434 2579
Fragments arranged by size:
2579 1434 1141 675 517 57
Bpu10I CC'TnA_GC
Cuts at: 2060 2297 2060
Size: 237 6166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3338 4528 5393 5691 3338
Size: 1190 865 298 4050
Fragments arranged by size:
4050 1190 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2591 2969 5066 882
Size: 617 1092 378 2097 2219
Fragments arranged by size:
2219 2097 1092 617 378
BsaAI yAC'GTr
Cuts at: 493 1204 3259 4194 493
Size: 711 2055 935 2702
Fragments arranged by size:
2702 2055 935 711
BsaBI GATnn'nnATC
Cuts at: 3101 6397 3101
Size: 3296 3107
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3039 3438 3600 4210
Size: 53 83 186 936 1506 399 162 610
Cuts at: 4210 4592 5227 275
Size: 382 635 1451
Fragments arranged by size:
1506 1451 936 635 610 399 382 186
162 83 53
BsaWI w'CCGG_w
Cuts at: 4836 5234 5508 5655 6066 4836
Size: 398 274 147 411 5173
Fragments arranged by size:
5173 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6178
Fragments arranged by size:
6178 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2488 2879 3341 1186
Size: 1045 257 391 462 4248
Fragments arranged by size:
4248 1045 462 391 257
BseYI C'CCAG_C
Cuts at: 2194 3148 5606 6102 2194
Size: 954 2458 496 2495
Fragments arranged by size:
2495 2458 954 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2276 2279 2329 2372 2606 1163
Size: 573 540 3 50 43 234 4960
Fragments arranged by size:
4960 573 540 234 50 43 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2076 3853 4614 5642 1540
Size: 275 186 75 1777 761 1028 2301
Fragments arranged by size:
2301 1777 1028 761 275 186 75
BsiHKAI G_wGCw'C
Cuts at: 4464 4549 5620 4464
Size: 85 1071 5247
Fragments arranged by size:
5247 1071 85
BsmI GAATG_Cn'
Cuts at: 3914 4007 3914
Size: 93 6310
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2187 2247 3144 3167 3382 3722
Size: 399 282 115 60 897 23 215 340
Cuts at: 3722 4464 4549 5620 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 897 742 399 340 282 215
115 85 60 23
BspEI T'CCGG_A
Cuts at: 6066 6066
Size: 6403
BspHI T'CATG_A
Cuts at: 4292 6022 4292
Size: 1730 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2628 2805 3345 3758 844
Size: 1784 177 540 413 3489
Fragments arranged by size:
3489 1784 540 413 177
BsrBI CCG'CTC
Cuts at: 1394 1823 4290 1394
Size: 429 2467 3507
Fragments arranged by size:
3507 2467 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4898 5072 65
Size: 1183 3650 174 1396
Fragments arranged by size:
3650 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2810 3474 5047 5234 1700
Size: 110 1000 664 1573 187 2869
Fragments arranged by size:
2869 1573 1000 664 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6403
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6403
BssSI C'ACGA_G
Cuts at: 3530 4463 5475 3530
Size: 933 1012 4458
Fragments arranged by size:
4458 1012 933
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 3115 3577 794
Size: 1472 849 462 3620
Fragments arranged by size:
3620 1472 849 462
BstBI TT'CG_AA
Cuts at: 5207 5207
Size: 6403
BstEII G'GTnAC_C
Cuts at: 1184 2813 1184
Size: 1629 4774
BstYI r'GATC_y
Cuts at: 1972 2052 2355 2724 3111 3733 4100 4485 4502
Size: 80 303 369 387 622 367 385 17
Cuts at: 4502 5954 6040 6052 6398 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 622 387 385 369 367 346
303 86 80 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3132 3659 513
Size: 552 939 1128 527 3257
Fragments arranged by size:
3257 1128 939 552 527
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5664
Fragments arranged by size:
5664 683 56
BtsI GCAGTG_nn'
Cuts at: 3034 3919 4713 4733 3034
Size: 885 794 20 4704
Fragments arranged by size:
4704 885 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6403
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6368
DraI TTT'AAA
Cuts at: 3707 3707
Size: 6403
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2042 5410 809
Size: 507 726 3368 1802
Fragments arranged by size:
3368 1802 726 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2085 2199
Size: 54 1425 50 275 18 243 12 114
Cuts at: 2199 2766 2820 2985 3294 3456 3516 3588 3850
Size: 567 54 165 309 162 60 72 262
Cuts at: 3850 4739 6368 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 567 309 275 262 243
165 162 114 72 60 54 54 50
43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2073 3850 1537
Size: 275 261 1777 4090
Fragments arranged by size:
4090 1777 275 261
EarI CTCTTCn'nnn_
Cuts at: 1353 4333 1353
Size: 2980 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3482 4990 5364 5510 387
Size: 1064 138 1893 1508 374 146 1280
Fragments arranged by size:
1893 1508 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2380 2437 2954 3541 3629 4466 5063
Size: 393 748 57 517 587 88 837 597
Cuts at: 5063 5850 6118 1239
Size: 787 268 1524
Fragments arranged by size:
1524 837 787 748 597 587 517 393
268 88 57
EcoO109I rG'GnC_Cy
Cuts at: 2287 3140 2287
Size: 853 5550
EcoRI G'AATT_C
Cuts at: 3712 4090 3712
Size: 378 6025
EcoRV GAT'ATC
Cuts at: 2638 2638
Size: 6403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2923 2923
Size: 6403
FspI TGC'GCA
Cuts at: 3390 4909 3390
Size: 1519 4884
FspAI rTGC'GCAy
Cuts at: 3390 3390
Size: 6403
HaeII r_GCGC'y
Cuts at: 1678 3042 3622 5550 1678
Size: 1364 580 1928 2531
Fragments arranged by size:
2531 1928 1364 580
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 2672 2704 3204
Size: 32 135 32 1055 32 13 32 500
Cuts at: 3204 3236 5044 5076 5118 5150 1373
Size: 32 1808 32 42 32 2626
Fragments arranged by size:
2626 1808 1055 500 135 42 32 32
32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2701 3544 3747 3993 669
Size: 988 281 763 843 203 246 3079
Fragments arranged by size:
3079 988 843 763 281 246 203
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6403
HpaI GTT'AAC
Cuts at: 3993 3993
Size: 6403
KasI G'GCGC_C
Cuts at: 3038 3038
Size: 6403
KpnI G_GTAC'C
Cuts at: 2320 3253 3728 2320
Size: 933 475 4995
Fragments arranged by size:
4995 933 475
MfeI C'AATT_G
Cuts at: 4002 4002
Size: 6403
MluI A'CGCG_T
Cuts at: 6279 6279
Size: 6403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2940 5517 5701 1305
Size: 1635 2577 184 2007
Fragments arranged by size:
2577 2007 1635 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3458 3518 10
Size: 54 1425 343 1626 60 2895
Fragments arranged by size:
2895 1626 1425 343 60 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2759 3224 3534 4361 4720 4879 518
Size: 648 1593 465 310 827 359 159 2042
Fragments arranged by size:
2042 1593 827 648 465 359 310 159
MspA1I CmG'CkG
Cuts at: 1606 2198 2227 2956 3462 3819 4496 5644 5889
Size: 592 29 729 506 357 677 1148 245
Cuts at: 5889 1606
Size: 2120
Fragments arranged by size:
2120 1148 729 677 592 506 357 245
29
NarI GG'CG_CC
Cuts at: 3039 3039
Size: 6403
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4912
Fragments arranged by size:
4912 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6403
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6403
NotI GC'GGCC_GC
Cuts at: 3850 3850
Size: 6403
NspI r_CATG'y
Cuts at: 1132 1229 2404 2971 3761 5306 1132
Size: 97 1175 567 790 1545 2229
Fragments arranged by size:
2229 1545 1175 790 567 97
PasI CC'CwG_GG
Cuts at: 1348 3001 1348
Size: 1653 4750
PciI A'CATG_T
Cuts at: 5302 5302
Size: 6403
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2208 2683 1108
Size: 1100 475 4828
Fragments arranged by size:
4828 1100 475
PfoI T'CCnGG_A
Cuts at: 2676 3432 2676
Size: 756 5647
PmeI GTTT'AAAC
Cuts at: 3707 3707
Size: 6403
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4472 4504 5985 6017 4472
Size: 32 1481 32 4858
Fragments arranged by size:
4858 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2287 2287
Size: 6403
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6403
PsiI TTA'TAA
Cuts at: 3973 3973
Size: 6403
PspOMI G'GGCC_C
Cuts at: 2068 3140 2068
Size: 1072 5331
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2204 2236 2204
Size: 32 6371
PstI C_TGCA'G
Cuts at: 830 2777 3528 3755 830
Size: 1947 751 227 3478
Fragments arranged by size:
3478 1947 751 227
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6403
PvuII CAG'CTG
Cuts at: 2956 2956
Size: 6403
SalI G'TCGA_C
Cuts at: 1936 3745 1936
Size: 1809 4594
SbfI CC_TGCA'GG
Cuts at: 3755 3755
Size: 6403
ScaI AGT'ACT
Cuts at: 4651 6363 4651
Size: 1712 4691
SexAI A'CCwGG_T
Cuts at: 2166 2283 2166
Size: 117 6286
SfcI C'TryA_G
Cuts at: 826 1045 2773 3524 3751 4886 5567 5758 826
Size: 219 1728 751 227 1135 681 191 1471
Fragments arranged by size:
1728 1471 1135 751 681 227 219 191
SfoI GGC'GCC
Cuts at: 3040 3040
Size: 6403
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6403
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6403
SmaI CCC'GGG
Cuts at: 1872 3730 1872
Size: 1858 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3353 4507 5408 5670 820
Size: 197 926 1410 1154 901 262 1553
Fragments arranged by size:
1553 1410 1154 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4194 493
Size: 3701 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6403
SphI G_CATG'C
Cuts at: 1132 2971 3761 1132
Size: 1839 790 3774
Fragments arranged by size:
3774 1839 790
SspI AAT'ATT
Cuts at: 5 52 4327 5
Size: 47 4275 2081
Fragments arranged by size:
4275 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6403
StyI C'CwwG_G
Cuts at: 513 1065 2004 2337 2898 2979 3842 513
Size: 552 939 333 561 81 863 3074
Fragments arranged by size:
3074 939 863 561 552 333 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2208 2441 3140 4430 4447 4600 4785
Size: 1255 628 233 699 1290 17 153 185
Cuts at: 4785 5230 325
Size: 445 1498
Fragments arranged by size:
1498 1290 1255 699 628 445 233 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4649 6361 96
Size: 274 80 33 51 1443 2672 1712 138
Fragments arranged by size:
2672 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6363 6395 6363
Size: 32 6371
Tth111I GACn'n_nGTC
Cuts at: 1316 2285 3194 1316
Size: 969 909 4525
Fragments arranged by size:
4525 969 909
XbaI T'CTAG_A
Cuts at: 3739 3739
Size: 6403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3286 3286
Size: 6403
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6403
XmaI C'CCGG_G
Cuts at: 1870 3728 1870
Size: 1858 4545
XmnI GAAnn'nnTTC
Cuts at: 4532 4532
Size: 6403
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3439 4211 276
Size: 53 83 186 936 1905 772 2468
Fragments arranged by size:
2468 1905 936 772 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII
SacI SacII SanDI SapI SfiI SrfI SwaI