Restriction map for
pFN21AB8123
(Circular) (Six-base) MAPSORT of: pFN21AB8123.seq Check: 3700 from: 1 to: 6256
pFN21AB8123 6256 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2440 2440
Size: 6256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2585 4066 278
Size: 53 83 186 936 1049 1481 2468
Fragments arranged by size:
2468 1481 1049 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2353 2859 3548 3599 1937
Size: 416 506 689 51 4594
Fragments arranged by size:
4594 689 506 416 51
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2772 3315 4319 5703 5971 1632
Size: 1140 543 1004 1384 268 1917
Fragments arranged by size:
1917 1384 1140 1004 543 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 3999 5571 3999
Size: 1572 4684
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 2043 3308 3356 3407 3565 3791 3943 6052
Size: 550 1265 48 51 158 226 152 2109
Cuts at: 6052 1493
Size: 1697
Fragments arranged by size:
2109 1697 1265 550 226 158 152 51
48
AseI AT'TA_AT
Cuts at: 160 1032 2337 4810 160
Size: 872 1305 2473 1606
Fragments arranged by size:
2473 1606 1305 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 2880 3581 1870
Size: 73 937 701 4545
Fragments arranged by size:
4545 937 701 73
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2495 2962 3577 5032 618
Size: 325 217 627 708 467 615 1455 1842
Fragments arranged by size:
1842 1455 708 627 615 467 325 217
BanII G_rGCy'C
Cuts at: 1391 2942 3575 1391
Size: 1551 633 4072
Fragments arranged by size:
4072 1551 633
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2194 2760 928
Size: 971 295 566 4424
Fragments arranged by size:
4424 971 566 295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4447 4481 1903
Size: 34 2510 34 3678
Fragments arranged by size:
3678 2510 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3183 4148 5358 3183
Size: 965 1210 4081
Fragments arranged by size:
4081 1210 965
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BfrBI ATG'CAT
Cuts at: 3209 3209
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4867 136
Size: 107 122 71 631 605 3195 1525
Fragments arranged by size:
3195 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 3673 3673
Size: 6256
Bme1580I G_kGCm'C
Cuts at: 1790 2667 4317 5473 1790
Size: 877 1650 1156 2573
Fragments arranged by size:
2573 1650 1156 877
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2736 3136 3987
Size: 416 53 83 399 24 1319 400 851
Cuts at: 3987 4945 442
Size: 958 1753
Fragments arranged by size:
1753 1319 958 851 416 400 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2409 2571 2882 2952 4916 1239
Size: 1170 162 311 70 1964 2579
Fragments arranged by size:
2579 1964 1170 311 162 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2614 3023 4381 5246 5544 2614
Size: 409 1358 865 298 3326
Fragments arranged by size:
3326 1358 865 409 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4919 882
Size: 617 3420 2219
Fragments arranged by size:
3420 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4047 493
Size: 711 2843 2702
Fragments arranged by size:
2843 2702 711
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2582 4063 4445 5080
Size: 53 83 186 936 1049 1481 382 635
Cuts at: 5080 275
Size: 1451
Fragments arranged by size:
1481 1451 1049 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4689 5087 5361 5508 5919 4689
Size: 398 274 147 411 5026
Fragments arranged by size:
5026 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2382 2412 2488 2518 3092
Size: 30 165 30 979 30 76 30 574
Cuts at: 3092 3122 1178
Size: 30 4312
Fragments arranged by size:
4312 979 574 165 76 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2241 2491 2496 1186
Size: 856 199 250 5 4946
Fragments arranged by size:
4946 856 250 199 5
BseYI C'CCAG_C
Cuts at: 5459 5955 5459
Size: 496 5760
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2075 2707 3104 1163
Size: 573 339 632 397 4315
Fragments arranged by size:
4315 632 573 397 339
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3706 4467 5495 1540
Size: 275 186 1705 761 1028 2301
Fragments arranged by size:
2301 1705 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2431 2476 4317 4402 5473 2431
Size: 45 1841 85 1071 3214
Fragments arranged by size:
3214 1841 1071 85 45
BsmI GAATG_Cn'
Cuts at: 2389 2695 3438 3767 3860 2389
Size: 306 743 329 93 4785
Fragments arranged by size:
4785 743 329 306 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2589 2589
Size: 6256
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2431 2476 2667 2942 3575 4317 4402
Size: 399 641 45 191 275 633 742 85
Cuts at: 4402 5473 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 641 633 399 275 191
85 45
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2440 3060 3611 844
Size: 1596 620 551 3489
Fragments arranged by size:
3489 1596 620 551
BsrBI CCG'CTC
Cuts at: 1394 1823 2282 4143 1394
Size: 429 459 1861 3507
Fragments arranged by size:
3507 1861 459 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2703 4751 4925 65
Size: 1183 1455 2048 174 1396
Fragments arranged by size:
2048 1455 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4900 5087 1700
Size: 110 3090 187 2869
Fragments arranged by size:
3090 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 3532 4316 5328 3532
Size: 784 1012 4460
Fragments arranged by size:
4460 1012 784
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 2063 2176 1184
Size: 879 113 5264
Fragments arranged by size:
5264 879 113
BstXI CCAn_nnnn'nTGG
Cuts at: 2492 2492
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 3033 3586 3953 4338 4355 5807 5893 5905
Size: 1061 553 367 385 17 1452 86 12
Cuts at: 5905 6251 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1061 553 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3549 3549
Size: 6256
Bsu36I CC'TnA_GG
Cuts at: 2933 2933
Size: 6256
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4765
Fragments arranged by size:
4765 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2623 487
Size: 683 56 1397 4120
Fragments arranged by size:
4120 1397 683 56
BtsI GCAGTG_nn'
Cuts at: 2497 3433 3772 4566 4586 2497
Size: 936 339 794 20 4167
Fragments arranged by size:
4167 936 794 339 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 2421 3560 2421
Size: 1139 5117
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2576 3703 4592
Size: 54 1425 50 275 18 746 1127 889
Cuts at: 4592 6221 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1127 889 746 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4186 1353
Size: 2833 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4843 5217 5363 387
Size: 1064 138 3254 374 146 1280
Fragments arranged by size:
3254 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2409 2571 2772 2882 2952 3315 4319
Size: 393 777 162 201 110 70 363 1004
Cuts at: 4319 4916 5703 5971 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1004 787 777 597 393 363 268
201 162 110 70
EcoO109I rG'GnC_Cy
Cuts at: 3103 3103
Size: 6256
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 5403 1678
Size: 3725 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 4897 4929 4971
Size: 32 135 32 974 32 2319 32 42
Cuts at: 4971 5003 1373
Size: 32 2626
Fragments arranged by size:
2626 2319 974 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2764 3600 3846 669
Size: 988 281 826 836 246 3079
Fragments arranged by size:
3079 988 836 826 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6256
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3243 3855 3243
Size: 612 5644
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3384 5370 5554 1305
Size: 2079 1986 184 2007
Fragments arranged by size:
2079 2007 1986 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4434
Fragments arranged by size:
4434 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 2339 2563 2708 4214 4573 4732
Size: 648 945 228 224 145 1506 359 159
Cuts at: 4732 518
Size: 2042
Fragments arranged by size:
2042 1506 945 648 359 228 224 159
145
MspA1I CmG'CkG
Cuts at: 1606 3672 4349 5497 5742 1606
Size: 2066 677 1148 245 2120
Fragments arranged by size:
2120 2066 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4765
Fragments arranged by size:
4765 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NsiI A_TGCA'T
Cuts at: 3211 3211
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 2472 3213 3614 5159 1132
Size: 97 1243 741 401 1545 2229
Fragments arranged by size:
2229 1545 1243 741 401 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6256
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6256
PfoI T'CCnGG_A
Cuts at: 2118 2898 2118
Size: 780 5476
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3103 3103
Size: 6256
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PstI C_TGCA'G
Cuts at: 830 2058 3608 830
Size: 1228 1550 3478
Fragments arranged by size:
3478 1550 1228
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SbfI CC_TGCA'GG
Cuts at: 3608 3608
Size: 6256
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SfcI C'TryA_G
Cuts at: 826 1045 2054 2321 3604 4739 5420 5611 826
Size: 219 1009 267 1283 1135 681 191 1471
Fragments arranged by size:
1471 1283 1135 1009 681 267 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 2882 3583 1872
Size: 1010 701 4545
Fragments arranged by size:
4545 1010 701
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2593 3038 4360 5261 5523 820
Size: 197 926 650 445 1322 901 262 1553
Fragments arranged by size:
1553 1322 926 901 650 445 262 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SspI AAT'ATT
Cuts at: 5 52 2815 4180 5
Size: 47 2763 1365 2081
Fragments arranged by size:
2763 2081 1365 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6256
StyI C'CwwG_G
Cuts at: 513 1065 2004 2268 3459 3695 513
Size: 552 939 264 1191 236 3074
Fragments arranged by size:
3074 1191 939 552 264 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3075 4283 4300 4453 4638 5083 325
Size: 1255 1495 1208 17 153 185 445 1498
Fragments arranged by size:
1498 1495 1255 1208 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2910 3108 4502
Size: 274 80 33 51 1443 933 198 1394
Cuts at: 4502 6214 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1394 933 274 198 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2382 2414 2421 2453 2488 2520 6216 6248 2382
Size: 32 7 32 35 32 3696 32 2390
Fragments arranged by size:
3696 2390 35 32 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6256
XbaI T'CTAG_A
Cuts at: 3175 3592 3175
Size: 417 5839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2595 2976 2595
Size: 381 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 2880 3581 1870
Size: 1010 701 4545
Fragments arranged by size:
4545 1010 701
XmnI GAAnn'nnTTC
Cuts at: 2367 3005 4385 2367
Size: 638 1380 4238
Fragments arranged by size:
4238 1380 638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2583 4064 276
Size: 53 83 186 936 1049 1481 2468
Fragments arranged by size:
2468 1481 1049 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI