Restriction map for
pFN21AB8111
(Circular) (Six-base) MAPSORT of: pFN21AB8111.seq Check: 4567 from: 1 to: 6373
pFN21AB8111 6373 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3154 3154
Size: 6373
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4183 278
Size: 53 83 186 936 2647 2468
Fragments arranged by size:
2647 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3716 1937
Size: 1779 4594
Acc65I G'GTAC_C
Cuts at: 3694 3694
Size: 6373
AclI AA'CG_TT
Cuts at: 1563 4500 4873 1563
Size: 2937 373 3063
Fragments arranged by size:
3063 2937 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4436 5820 6088 1632
Size: 2804 1384 268 1917
Fragments arranged by size:
2804 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6176
AflIII A'CryG_T
Cuts at: 1203 5272 6249 1203
Size: 4069 977 1327
Fragments arranged by size:
4069 1327 977
AgeI A'CCGG_T
Cuts at: 5204 5204
Size: 6373
AhdI GACnn_n'nnGTC
Cuts at: 5102 5102
Size: 6373
AleI CACnn'nnGTG
Cuts at: 1166 3604 1166
Size: 2438 3935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6373
AlwNI CAG_nnn'CTG
Cuts at: 4116 5688 4116
Size: 1572 4801
ApaI G_GGCC'C
Cuts at: 2150 3389 2150
Size: 1239 5134
ApaLI G'TGCA_C
Cuts at: 4430 5586 4430
Size: 1156 5217
ApoI r'AATT_y
Cuts at: 1493 2337 2915 3495 3532 3682 3908 4060 6169
Size: 844 578 580 37 150 226 152 2109
Cuts at: 6169 1493
Size: 1697
Fragments arranged by size:
2109 1697 844 580 578 226 152 150
37
AseI AT'TA_AT
Cuts at: 160 1032 4927 160
Size: 872 3895 1606
Fragments arranged by size:
3895 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
AvaI C'yCGr_G
Cuts at: 1870 1943 2301 3698 1870
Size: 73 358 1397 4545
Fragments arranged by size:
4545 1397 358 73
BamHI G'GATC_C
Cuts at: 3466 3703 4070 3466
Size: 237 367 5769
Fragments arranged by size:
5769 367 237
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2186 3348 3694 5149 618
Size: 325 217 627 399 1162 346 1455 1842
Fragments arranged by size:
1842 1455 1162 627 399 346 325 217
BanII G_rGCy'C
Cuts at: 1391 2150 2806 3389 3692 1391
Size: 759 656 583 303 4072
Fragments arranged by size:
4072 759 656 583 303
BbeI G_GCGC'C
Cuts at: 2190 2190
Size: 6373
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3111 3162 928
Size: 971 1212 51 4139
Fragments arranged by size:
4139 1212 971 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4564 4598 1903
Size: 34 2627 34 3678
Fragments arranged by size:
3678 2627 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2993 4265 5475 2993
Size: 1272 1210 3891
Fragments arranged by size:
3891 1272 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6373
BfrBI ATG'CAT
Cuts at: 2814 2814
Size: 6373
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3153 4984 136
Size: 107 122 71 631 605 1481 1831 1525
Fragments arranged by size:
1831 1525 1481 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6368 6368
Size: 6373
BlpI GC'TnA_GC
Cuts at: 3790 3790
Size: 6373
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2150 3389 4434 5590 1790
Size: 253 107 1239 1045 1156 2573
Fragments arranged by size:
2573 1239 1156 1045 253 107
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6373
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3383 4104 5062
Size: 416 53 83 399 24 1966 721 958
Cuts at: 5062 442
Size: 1753
Fragments arranged by size:
1966 1753 958 721 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2302 2363 2739 5033 1239
Size: 1063 61 376 2294 2579
Fragments arranged by size:
2579 2294 1063 376 61
Bpu10I CC'TnA_GC
Cuts at: 3143 3143
Size: 6373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4498 5363 5661 4498
Size: 865 298 5210
Fragments arranged by size:
5210 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2534 2928 5036 882
Size: 617 1035 394 2108 2219
Fragments arranged by size:
2219 2108 1035 617 394
BsaAI yAC'GTr
Cuts at: 493 1204 3042 4164 493
Size: 711 1838 1122 2702
Fragments arranged by size:
2702 1838 1122 711
BsaBI GATnn'nnATC
Cuts at: 6367 6367
Size: 6373
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 2187 3065 3660
Size: 53 83 186 936 501 153 878 595
Cuts at: 3660 4180 4562 5197 275
Size: 520 382 635 1451
Fragments arranged by size:
1451 936 878 635 595 520 501 382
186 153 83 53
BsaWI w'CCGG_w
Cuts at: 2626 4806 5204 5478 5625 6036 2626
Size: 2180 398 274 147 411 2963
Fragments arranged by size:
2963 2180 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3254 3284 1178
Size: 30 165 30 1851 30 4267
Fragments arranged by size:
4267 1851 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3169 3172 1186
Size: 1983 3 4387
Fragments arranged by size:
4387 1983 3
BseYI C'CCAG_C
Cuts at: 2208 3587 5576 6072 2208
Size: 1379 1989 496 2509
Fragments arranged by size:
2509 1989 1379 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3038 3631 3689 1163
Size: 573 1302 593 58 3847
Fragments arranged by size:
3847 1302 593 573 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3823 4584 5612 1540
Size: 275 186 1822 761 1028 2301
Fragments arranged by size:
2301 1822 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4434 4519 5590 4434
Size: 85 1071 5217
Fragments arranged by size:
5217 1071 85
BsmI GAATG_Cn'
Cuts at: 2124 3884 3977 2124
Size: 1760 93 4520
Fragments arranged by size:
4520 1760 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2150 2806 3389 3692 4434 4519
Size: 399 253 107 656 583 303 742 85
Cuts at: 4519 5590 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 656 583 399 303 253
107 85
BspEI T'CCGG_A
Cuts at: 6036 6036
Size: 6373
BspHI T'CATG_A
Cuts at: 4262 5992 4262
Size: 1730 4643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3060 3096 3154 3661 3728 844
Size: 2216 36 58 507 67 3489
Fragments arranged by size:
3489 2216 507 67 58 36
BsrBI CCG'CTC
Cuts at: 1394 1823 2395 4260 1394
Size: 429 572 1865 3507
Fragments arranged by size:
3507 1865 572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4868 5042 65
Size: 1183 3620 174 1396
Fragments arranged by size:
3620 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2189 3091 5017 5204 1700
Size: 110 379 902 1926 187 2869
Fragments arranged by size:
2869 1926 902 379 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6373
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6373
BssSI C'ACGA_G
Cuts at: 2939 4433 5445 2939
Size: 1494 1012 3867
Fragments arranged by size:
3867 1494 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6373
BstBI TT'CG_AA
Cuts at: 5177 5177
Size: 6373
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6373
BstYI r'GATC_y
Cuts at: 1972 3466 3618 3703 4070 4455 4472 5924 6010
Size: 1494 152 85 367 385 17 1452 86
Cuts at: 6010 6022 6368 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1494 1452 385 367 346 152 86
85 17 12
Bsu36I CC'TnA_GG
Cuts at: 2241 2925 2241
Size: 684 5689
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 2286 513
Size: 552 939 150 132 4600
Fragments arranged by size:
4600 939 552 150 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5634
Fragments arranged by size:
5634 683 56
BtsI GCAGTG_nn'
Cuts at: 2163 3889 4683 4703 2163
Size: 1726 794 20 3833
Fragments arranged by size:
3833 1726 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6373
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6338
DraI TTT'AAA
Cuts at: 3538 3677 3538
Size: 139 6234
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5380 809
Size: 507 4064 1802
Fragments arranged by size:
4064 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3820 4709
Size: 54 1425 50 275 18 177 1813 889
Cuts at: 4709 6338 8
Size: 1629 43
Fragments arranged by size:
1813 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3820 1537
Size: 275 2008 4090
Fragments arranged by size:
4090 2008 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3302 4303 1353
Size: 1949 1001 3423
Fragments arranged by size:
3423 1949 1001
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2205 2767 3351 4960 5334 5480
Size: 1064 138 616 562 584 1609 374 146
Cuts at: 5480 387
Size: 1280
Fragments arranged by size:
1609 1280 1064 616 584 562 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2302 2363 2739 4436 5033 5820 6088
Size: 393 670 61 376 1697 597 787 268
Cuts at: 6088 1239
Size: 1524
Fragments arranged by size:
1697 1524 787 670 597 393 376 268
61
EcoO109I rG'GnC_Cy
Cuts at: 2094 2146 2147 2374 2617 2094
Size: 52 1 227 243 5850
Fragments arranged by size:
5850 243 227 52 1
EcoRI G'AATT_C
Cuts at: 3682 4060 3682
Size: 378 5995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 6373
FspI TGC'GCA
Cuts at: 4879 4879
Size: 6373
HaeII r_GCGC'y
Cuts at: 1678 2190 5520 1678
Size: 512 3330 2531
Fragments arranged by size:
3330 2531 512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2390 2422 2558 2590 3248
Size: 32 135 32 818 32 136 32 658
Cuts at: 3248 3254 3280 3286 3559 3591 5014 5046 5088
Size: 6 26 6 273 32 1423 32 42
Cuts at: 5088 5120 1373
Size: 32 2626
Fragments arranged by size:
2626 1423 818 658 273 136 135 42
32 32 32 32 32 32 32 26
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3475 3717 3963 669
Size: 988 281 1537 242 246 3079
Fragments arranged by size:
3079 1537 988 281 246 242
HindIII A'AGCT_T
Cuts at: 748 2435 748
Size: 1687 4686
HpaI GTT'AAC
Cuts at: 3963 3963
Size: 6373
KasI G'GCGC_C
Cuts at: 2186 2186
Size: 6373
KpnI G_GTAC'C
Cuts at: 3698 3698
Size: 6373
MfeI C'AATT_G
Cuts at: 3972 3972
Size: 6373
MluI A'CGCG_T
Cuts at: 6249 6249
Size: 6373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2874 3081 3228 3300 5487 5671 1305
Size: 1569 207 147 72 2187 184 2007
Fragments arranged by size:
2187 2007 1569 207 184 147 72
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4374
Fragments arranged by size:
4374 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3164 3604 4331 4690 4849 518
Size: 648 1998 440 727 359 159 2042
Fragments arranged by size:
2042 1998 727 648 440 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 3430 3591 3665 3789 4466 5614 5859
Size: 420 1404 161 74 124 677 1148 245
Cuts at: 5859 1606
Size: 2120
Fragments arranged by size:
2120 1404 1148 677 420 245 161 124
74
NaeI GCC'GGC
Cuts at: 2191 3093 2191
Size: 902 5471
NarI GG'CG_CC
Cuts at: 2187 2187
Size: 6373
NcoI C'CATG_G
Cuts at: 513 1065 2004 2154 513
Size: 552 939 150 4732
Fragments arranged by size:
4732 939 552 150
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6373
NgoMIV G'CCGG_C
Cuts at: 2189 3091 2189
Size: 902 5471
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6373
NotI GC'GGCC_GC
Cuts at: 3820 3820
Size: 6373
NsiI A_TGCA'T
Cuts at: 2816 2816
Size: 6373
NspI r_CATG'y
Cuts at: 1132 1229 2207 3163 3731 5276 1132
Size: 97 978 956 568 1545 2229
Fragments arranged by size:
2229 1545 978 956 568 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6373
PciI A'CATG_T
Cuts at: 5272 5272
Size: 6373
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2830 1108
Size: 1722 4651
PfoI T'CCnGG_A
Cuts at: 3621 3621
Size: 6373
PmeI GTTT'AAAC
Cuts at: 3677 3677
Size: 6373
PmlI CAC'GTG
Cuts at: 3042 3042
Size: 6373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4442 4474 5955 5987 4442
Size: 32 1481 32 4828
Fragments arranged by size:
4828 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2094 2374 2617 2094
Size: 280 243 5850
Fragments arranged by size:
5850 280 243
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6373
PsiI TTA'TAA
Cuts at: 3943 3943
Size: 6373
PspOMI G'GGCC_C
Cuts at: 2146 3385 2146
Size: 1239 5134
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3270 3302 3270
Size: 32 6341
PstI C_TGCA'G
Cuts at: 830 2170 2202 3725 830
Size: 1340 32 1523 3478
Fragments arranged by size:
3478 1523 1340 32
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6373
PvuII CAG'CTG
Cuts at: 2026 3430 2026
Size: 1404 4969
SalI G'TCGA_C
Cuts at: 1936 3715 1936
Size: 1779 4594
SbfI CC_TGCA'GG
Cuts at: 3725 3725
Size: 6373
ScaI AGT'ACT
Cuts at: 2588 4621 6333 2588
Size: 2033 1712 2628
Fragments arranged by size:
2628 2033 1712
SfcI C'TryA_G
Cuts at: 826 1045 2166 2198 3721 4856 5537 5728 826
Size: 219 1121 32 1523 1135 681 191 1471
Fragments arranged by size:
1523 1471 1135 1121 681 219 191 32
SfoI GGC'GCC
Cuts at: 2188 2188
Size: 6373
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6373
SmaI CCC'GGG
Cuts at: 1872 3700 1872
Size: 1828 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2301 4477 5378 5640 820
Size: 197 926 358 2176 901 262 1553
Fragments arranged by size:
2176 1553 926 901 358 262 197
SnaBI TAC'GTA
Cuts at: 493 4164 493
Size: 3671 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6373
SphI G_CATG'C
Cuts at: 1132 2207 3163 3731 1132
Size: 1075 956 568 3774
Fragments arranged by size:
3774 1075 956 568
SspI AAT'ATT
Cuts at: 5 52 4297 5
Size: 47 4245 2081
Fragments arranged by size:
4245 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6373
StyI C'CwwG_G
Cuts at: 513 1065 2004 2154 2266 3344 3500 3812 513
Size: 552 939 150 112 1078 156 312 3074
Fragments arranged by size:
3074 1078 939 552 312 156 150 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4400 4417 4570 4755 5200 325
Size: 1255 2820 17 153 185 445 1498
Fragments arranged by size:
2820 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2586 3246 4619
Size: 274 80 33 51 1443 609 660 1373
Cuts at: 4619 6331 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1373 660 609 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6333 6365 6333
Size: 32 6341
Tth111I GACn'n_nGTC
Cuts at: 1316 2104 1316
Size: 788 5585
XbaI T'CTAG_A
Cuts at: 3709 3709
Size: 6373
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2293 2728 2293
Size: 435 5938
XhoI C'TCGA_G
Cuts at: 1943 2301 1943
Size: 358 6015
XmaI C'CCGG_G
Cuts at: 1870 3698 1870
Size: 1828 4545
XmnI GAAnn'nnTTC
Cuts at: 4502 4502
Size: 6373
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4181 276
Size: 53 83 186 936 2647 2468
Fragments arranged by size:
2647 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NruI PacI PspXI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI