Restriction map for
pFN21AB8110
(Circular) (Six-base) MAPSORT of: pFN21AB8110.seq Check: 7928 from: 1 to: 5245
pFN21AB8110 5245 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3055 278
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2588 1937
Size: 651 4594
Acc65I G'GTAC_C
Cuts at: 2566 2566
Size: 5245
AclI AA'CG_TT
Cuts at: 1563 3372 3745 1563
Size: 1809 373 3063
Fragments arranged by size:
3063 1809 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2176 3308 4692 4960 1632
Size: 544 1132 1384 268 1917
Fragments arranged by size:
1917 1384 1132 544 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5048
AflIII A'CryG_T
Cuts at: 1203 4144 5121 1203
Size: 2941 977 1327
Fragments arranged by size:
2941 1327 977
AgeI A'CCGG_T
Cuts at: 4076 4076
Size: 5245
AhdI GACnn_n'nnGTC
Cuts at: 3974 3974
Size: 5245
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5245
AlwNI CAG_nnn'CTG
Cuts at: 2145 2988 4560 2145
Size: 843 1572 2830
Fragments arranged by size:
2830 1572 843
ApaI G_GGCC'C
Cuts at: 2515 2515
Size: 5245
ApaLI G'TGCA_C
Cuts at: 3302 4458 3302
Size: 1156 4089
ApoI r'AATT_y
Cuts at: 1493 2554 2780 2932 5041 1493
Size: 1061 226 152 2109 1697
Fragments arranged by size:
2109 1697 1061 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3799 160
Size: 872 2767 1606
Fragments arranged by size:
2767 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
AvaI C'yCGr_G
Cuts at: 1870 1943 2299 2495 2570 1870
Size: 73 356 196 75 4545
Fragments arranged by size:
4545 356 196 75 73
AvrII C'CTAG_G
Cuts at: 2179 2179
Size: 5245
BamHI G'GATC_C
Cuts at: 2429 2575 2942 2429
Size: 146 367 4732
Fragments arranged by size:
4732 367 146
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2314 2441 2566 4021 618
Size: 325 217 627 527 127 125 1455 1842
Fragments arranged by size:
1842 1455 627 527 325 217 127 125
BanII G_rGCy'C
Cuts at: 1391 2496 2515 2564 1391
Size: 1105 19 49 4072
Fragments arranged by size:
4072 1105 49 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4274
BbvCI CC'TCA_GC
Cuts at: 2538 2538
Size: 5245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3436 3470 1903
Size: 34 50 34 1415 34 3678
Fragments arranged by size:
3678 1415 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3137 4347 3137
Size: 1210 4035
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5245
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2142 3856 136
Size: 107 122 71 631 605 470 1714 1525
Fragments arranged by size:
1714 1525 631 605 470 122 107 71
BglII A'GATC_T
Cuts at: 5240 5240
Size: 5245
BlpI GC'TnA_GC
Cuts at: 2396 2662 2396
Size: 266 4979
Bme1580I G_kGCm'C
Cuts at: 1790 2300 2319 2515 3306 4462 1790
Size: 510 19 196 791 1156 2573
Fragments arranged by size:
2573 1156 791 510 196 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5245
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2260 2976 3934
Size: 416 53 83 399 24 843 716 958
Cuts at: 3934 442
Size: 1753
Fragments arranged by size:
1753 958 843 716 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 3905 1239
Size: 1073 1593 2579
Fragments arranged by size:
2579 1593 1073
Bpu10I CC'TnA_GC
Cuts at: 2490 2538 2490
Size: 48 5197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3370 4235 4533 3370
Size: 865 298 4082
Fragments arranged by size:
4082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3908 882
Size: 617 2409 2219
Fragments arranged by size:
2409 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3036 493
Size: 711 1832 2702
Fragments arranged by size:
2702 1832 711
BsaBI GATnn'nnATC
Cuts at: 5239 5239
Size: 5245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3052 3434 4069 275
Size: 53 83 186 936 1519 382 635 1451
Fragments arranged by size:
1519 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3678 4076 4350 4497 4908 3678
Size: 398 274 147 411 4015
Fragments arranged by size:
4015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2234 2264 1178
Size: 30 165 30 831 30 4159
Fragments arranged by size:
4159 831 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 1186
Size: 1138 4107
BseYI C'CCAG_C
Cuts at: 2433 4448 4944 2433
Size: 2015 496 2734
Fragments arranged by size:
2734 2015 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2333 2429 1163
Size: 573 597 96 3979
Fragments arranged by size:
3979 597 573 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2695 3456 4484 1540
Size: 275 186 694 761 1028 2301
Fragments arranged by size:
2301 1028 761 694 275 186
BsiHKAI G_wGCw'C
Cuts at: 3306 3391 4462 3306
Size: 85 1071 4089
Fragments arranged by size:
4089 1071 85
BsmI GAATG_Cn'
Cuts at: 2756 2849 2756
Size: 93 5152
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2300 2319 2496 2515 2564 3306 3391
Size: 399 510 19 177 19 49 742 85
Cuts at: 3391 4462 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 510 399 177 85 49
19 19
BspEI T'CCGG_A
Cuts at: 4908 4908
Size: 5245
BspHI T'CATG_A
Cuts at: 3134 4864 3134
Size: 1730 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2600 844
Size: 1756 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3132 1394
Size: 429 1309 3507
Fragments arranged by size:
3507 1309 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3740 3914 65
Size: 1183 2492 174 1396
Fragments arranged by size:
2492 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3889 4076 1700
Size: 110 2079 187 2869
Fragments arranged by size:
2869 2079 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5245
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5245
BssSI C'ACGA_G
Cuts at: 2306 2387 3305 4317 2306
Size: 81 918 1012 3234
Fragments arranged by size:
3234 1012 918 81
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5245
BstBI TT'CG_AA
Cuts at: 4049 4049
Size: 5245
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5245
BstXI CCAn_nnnn'nTGG
Cuts at: 2047 2047
Size: 5245
BstYI r'GATC_y
Cuts at: 1972 2343 2429 2575 2942 3327 3344 4796 4882
Size: 371 86 146 367 385 17 1452 86
Cuts at: 4882 4894 5240 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 371 367 346 146 86
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4693
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4506
Fragments arranged by size:
4506 683 56
BtsI GCAGTG_nn'
Cuts at: 2761 3555 3575 2761
Size: 794 20 4431
Fragments arranged by size:
4431 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5210
DraI TTT'AAA
Cuts at: 2549 2549
Size: 5245
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4252 809
Size: 507 2936 1802
Fragments arranged by size:
2936 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2692 3581 5210
Size: 54 1425 50 275 18 862 889 1629
Cuts at: 5210 8
Size: 43
Fragments arranged by size:
1629 1425 889 862 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2692 1537
Size: 275 880 4090
Fragments arranged by size:
4090 880 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2523 3175 1353
Size: 1170 652 3423
Fragments arranged by size:
3423 1170 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3832 4206 4352 387
Size: 1064 138 2243 374 146 1280
Fragments arranged by size:
2243 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2312 3308 3905 4692 4960 1239
Size: 393 544 136 996 597 787 268 1524
Fragments arranged by size:
1524 996 787 597 544 393 268 136
EcoO109I rG'GnC_Cy
Cuts at: 2014 2500 2511 2512 2014
Size: 486 11 1 4747
Fragments arranged by size:
4747 486 11 1
EcoRI G'AATT_C
Cuts at: 2554 2932 2554
Size: 378 4867
FspI TGC'GCA
Cuts at: 3751 3751
Size: 5245
HaeII r_GCGC'y
Cuts at: 1678 4392 1678
Size: 2714 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 3886 3918 3960
Size: 32 135 32 569 32 1713 32 42
Cuts at: 3960 3992 1373
Size: 32 2626
Fragments arranged by size:
2626 1713 569 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2589 2835 669
Size: 988 281 651 246 3079
Fragments arranged by size:
3079 988 651 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5245
HpaI GTT'AAC
Cuts at: 2835 2835
Size: 5245
KpnI G_GTAC'C
Cuts at: 2570 2570
Size: 5245
MfeI C'AATT_G
Cuts at: 2844 2844
Size: 5245
MluI A'CGCG_T
Cuts at: 5121 5121
Size: 5245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4359 4543 1305
Size: 3054 184 2007
Fragments arranged by size:
3054 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3423
Fragments arranged by size:
3423 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3203 3562 3721 518
Size: 648 2037 359 159 2042
Fragments arranged by size:
2042 2037 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2142 2661 3338 4486 4731 1606
Size: 536 519 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 536 519 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4693
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5245
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5245
NotI GC'GGCC_GC
Cuts at: 2692 2692
Size: 5245
NspI r_CATG'y
Cuts at: 1132 1229 2260 2603 4148 1132
Size: 97 1031 343 1545 2229
Fragments arranged by size:
2229 1545 1031 343 97
PasI CC'CwG_GG
Cuts at: 1348 2319 2507 1348
Size: 971 188 4086
Fragments arranged by size:
4086 971 188
PciI A'CATG_T
Cuts at: 4144 4144
Size: 5245
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2131 1108
Size: 1023 4222
PmeI GTTT'AAAC
Cuts at: 2549 2549
Size: 5245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 4827 4859 3314
Size: 32 1481 32 3700
Fragments arranged by size:
3700 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2014 2500 2014
Size: 486 4759
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5245
PsiI TTA'TAA
Cuts at: 2815 2815
Size: 5245
PspOMI G'GGCC_C
Cuts at: 2511 2511
Size: 5245
PstI C_TGCA'G
Cuts at: 830 2597 830
Size: 1767 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5245
SalI G'TCGA_C
Cuts at: 1936 2587 1936
Size: 651 4594
SbfI CC_TGCA'GG
Cuts at: 2597 2597
Size: 5245
ScaI AGT'ACT
Cuts at: 3493 5205 3493
Size: 1712 3533
SfcI C'TryA_G
Cuts at: 826 1045 2197 2353 2593 3728 4409 4600 826
Size: 219 1152 156 240 1135 681 191 1471
Fragments arranged by size:
1471 1152 1135 681 240 219 191 156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5245
SmaI CCC'GGG
Cuts at: 1872 2301 2572 1872
Size: 429 271 4545
Fragments arranged by size:
4545 429 271
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3349 4250 4512 820
Size: 197 926 1406 901 262 1553
Fragments arranged by size:
1553 1406 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3036 493
Size: 2543 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5245
SphI G_CATG'C
Cuts at: 1132 2260 2603 1132
Size: 1128 343 3774
Fragments arranged by size:
3774 1128 343
SrfI GCCC'GGGC
Cuts at: 2301 2301
Size: 5245
SspI AAT'ATT
Cuts at: 5 52 3169 5
Size: 47 3117 2081
Fragments arranged by size:
3117 2081 47
StuI AGG'CCT
Cuts at: 1517 2366 1517
Size: 849 4396
StyI C'CwwG_G
Cuts at: 513 1065 2179 2684 513
Size: 552 1114 505 3074
Fragments arranged by size:
3074 1114 552 505
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2077 2138 2459 3272 3289 3442 3627
Size: 1255 497 61 321 813 17 153 185
Cuts at: 3627 4072 325
Size: 445 1498
Fragments arranged by size:
1498 1255 813 497 445 321 185 153
61 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3491 5203 96
Size: 274 80 33 51 1443 1514 1712 138
Fragments arranged by size:
1712 1514 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2234 2266 5205 5237 2234
Size: 32 2939 32 2242
Fragments arranged by size:
2939 2242 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5245
XbaI T'CTAG_A
Cuts at: 2581 2581
Size: 5245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2071 2233 2471 2071
Size: 162 238 4845
Fragments arranged by size:
4845 238 162
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5245
XmaI C'CCGG_G
Cuts at: 1870 2299 2570 1870
Size: 429 271 4545
Fragments arranged by size:
4545 429 271
XmnI GAAnn'nnTTC
Cuts at: 3374 3374
Size: 5245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3053 276
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SwaI