Restriction map for
pFN21AB8109
(Circular) (Six-base) MAPSORT of: pFN21AB8109.seq Check: 1898 from: 1 to: 5164
pFN21AB8109 5164 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2974 278
Size: 53 83 186 936 1438 2468
Fragments arranged by size:
2468 1438 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2232 2507 1937
Size: 295 275 4594
Fragments arranged by size:
4594 295 275
Acc65I G'GTAC_C
Cuts at: 2485 2485
Size: 5164
AclI AA'CG_TT
Cuts at: 1563 3291 3664 1563
Size: 1728 373 3063
Fragments arranged by size:
3063 1728 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2427 3227 4611 4879 1632
Size: 795 800 1384 268 1917
Fragments arranged by size:
1917 1384 800 795 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4967
AflIII A'CryG_T
Cuts at: 1203 4063 5040 1203
Size: 2860 977 1327
Fragments arranged by size:
2860 1327 977
AgeI A'CCGG_T
Cuts at: 3995 3995
Size: 5164
AhdI GACnn_n'nnGTC
Cuts at: 3893 3893
Size: 5164
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5164
AlwNI CAG_nnn'CTG
Cuts at: 2907 4479 2907
Size: 1572 3592
ApaLI G'TGCA_C
Cuts at: 3221 4377 3221
Size: 1156 4008
ApoI r'AATT_y
Cuts at: 1493 2473 2699 2851 4960 1493
Size: 980 226 152 2109 1697
Fragments arranged by size:
2109 1697 980 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3718 160
Size: 872 2686 1606
Fragments arranged by size:
2686 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5164
AvaI C'yCGr_G
Cuts at: 1870 1943 2145 2489 1870
Size: 73 202 344 4545
Fragments arranged by size:
4545 344 202 73
BamHI G'GATC_C
Cuts at: 2494 2861 2494
Size: 367 4797
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2431 2485 3940 618
Size: 325 217 627 644 54 1455 1842
Fragments arranged by size:
1842 1455 644 627 325 217 54
BanII G_rGCy'C
Cuts at: 1391 2483 1391
Size: 1092 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2081 2092 928
Size: 971 182 11 4000
Fragments arranged by size:
4000 971 182 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3355 3389 1903
Size: 34 1418 34 3678
Fragments arranged by size:
3678 1418 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3056 4266 3056
Size: 1210 3954
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5164
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3775 136
Size: 107 122 71 631 605 2103 1525
Fragments arranged by size:
2103 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2175 5159 2175
Size: 2984 2180
BlpI GC'TnA_GC
Cuts at: 2581 2581
Size: 5164
Bme1580I G_kGCm'C
Cuts at: 1790 2258 2434 3225 4381 1790
Size: 468 176 791 1156 2573
Fragments arranged by size:
2573 1156 791 468 176
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5164
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2895 3853 442
Size: 416 53 83 399 24 1478 958 1753
Fragments arranged by size:
1753 1478 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3824 1239
Size: 2585 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3289 4154 4452 3289
Size: 865 298 4001
Fragments arranged by size:
4001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2449 3827 882
Size: 617 950 1378 2219
Fragments arranged by size:
2219 1378 950 617
BsaAI yAC'GTr
Cuts at: 493 1204 2955 493
Size: 711 1751 2702
Fragments arranged by size:
2702 1751 711
BsaBI GATnn'nnATC
Cuts at: 5158 5158
Size: 5164
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2971 3353 3988 275
Size: 53 83 186 936 1438 382 635 1451
Fragments arranged by size:
1451 1438 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3597 3995 4269 4416 4827 3597
Size: 398 274 147 411 3934
Fragments arranged by size:
3934 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2126 2156 1178
Size: 30 165 30 723 30 4186
Fragments arranged by size:
4186 723 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5164
BseYI C'CCAG_C
Cuts at: 4367 4863 4367
Size: 496 4668
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4591
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2064 2068 2119 2614 3375 4403
Size: 275 186 63 4 51 495 761 1028
Cuts at: 4403 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 495 275 186 63 51
4
BsiHKAI G_wGCw'C
Cuts at: 3225 3310 4381 3225
Size: 85 1071 4008
Fragments arranged by size:
4008 1071 85
BsmI GAATG_Cn'
Cuts at: 2675 2768 2675
Size: 93 5071
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2258 2434 2483 3225 3310 4381 1391
Size: 399 468 176 49 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 468 399 176 85 49
BspEI T'CCGG_A
Cuts at: 4827 4827
Size: 5164
BspHI T'CATG_A
Cuts at: 3053 4783 3053
Size: 1730 3434
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2519 844
Size: 1675 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3051 1394
Size: 429 1228 3507
Fragments arranged by size:
3507 1228 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3659 3833 65
Size: 1183 2411 174 1396
Fragments arranged by size:
2411 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3808 3995 1700
Size: 110 1998 187 2869
Fragments arranged by size:
2869 1998 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5164
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5164
BssSI C'ACGA_G
Cuts at: 3224 4236 3224
Size: 1012 4152
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5164
BstBI TT'CG_AA
Cuts at: 3968 3968
Size: 5164
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5164
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5164
BstYI r'GATC_y
Cuts at: 1972 2175 2494 2861 3246 3263 4715 4801 4813
Size: 203 319 367 385 17 1452 86 12
Cuts at: 4813 5159 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 319 203 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3673
Fragments arranged by size:
3673 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4425
Fragments arranged by size:
4425 683 56
BtsI GCAGTG_nn'
Cuts at: 2680 3474 3494 2680
Size: 794 20 4350
Fragments arranged by size:
4350 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5164
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5129
DraI TTT'AAA
Cuts at: 2468 2468
Size: 5164
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2371 4171 809
Size: 507 1055 1800 1802
Fragments arranged by size:
1802 1800 1055 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2611 3500
Size: 54 1425 50 275 18 286 495 889
Cuts at: 3500 5129 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 495 286 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2116 2611 1537
Size: 275 304 495 4090
Fragments arranged by size:
4090 495 304 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3094 1353
Size: 1741 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3751 4125 4271 387
Size: 1064 138 2162 374 146 1280
Fragments arranged by size:
2162 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2427 3227 3824 4611 4879 1239
Size: 393 795 800 597 787 268 1524
Fragments arranged by size:
1524 800 795 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2442 2442
Size: 5164
EcoRI G'AATT_C
Cuts at: 2473 2851 2473
Size: 378 4786
FspI TGC'GCA
Cuts at: 3670 3670
Size: 5164
HaeII r_GCGC'y
Cuts at: 1678 4311 1678
Size: 2633 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2158 3805 3837 3879
Size: 32 135 32 554 32 1647 32 42
Cuts at: 3879 3911 1373
Size: 32 2626
Fragments arranged by size:
2626 1647 554 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2508 2754 669
Size: 988 281 570 246 3079
Fragments arranged by size:
3079 988 570 281 246
HindIII A'AGCT_T
Cuts at: 748 2215 748
Size: 1467 3697
HpaI GTT'AAC
Cuts at: 2754 2754
Size: 5164
KpnI G_GTAC'C
Cuts at: 2489 2489
Size: 5164
MfeI C'AATT_G
Cuts at: 2763 2763
Size: 5164
MluI A'CGCG_T
Cuts at: 5040 5040
Size: 5164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4278 4462 1305
Size: 2973 184 2007
Fragments arranged by size:
2973 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3342
Fragments arranged by size:
3342 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2186 3122 3481 3640 518
Size: 648 843 177 936 359 159 2042
Fragments arranged by size:
2042 936 843 648 359 177 159
MspA1I CmG'CkG
Cuts at: 1606 2580 3257 4405 4650 1606
Size: 974 677 1148 245 2120
Fragments arranged by size:
2120 1148 974 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3673
Fragments arranged by size:
3673 939 552
NdeI CA'TA_TG
Cuts at: 387 2461 387
Size: 2074 3090
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5164
NotI GC'GGCC_GC
Cuts at: 2611 2611
Size: 5164
NspI r_CATG'y
Cuts at: 1132 1229 2082 2522 4067 1132
Size: 97 853 440 1545 2229
Fragments arranged by size:
2229 1545 853 440 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5164
PciI A'CATG_T
Cuts at: 4063 4063
Size: 5164
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2131 1108
Size: 1023 4141
PmeI GTTT'AAAC
Cuts at: 2468 2468
Size: 5164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 4746 4778 3233
Size: 32 1481 32 3619
Fragments arranged by size:
3619 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5164
PsiI TTA'TAA
Cuts at: 2734 2734
Size: 5164
PstI C_TGCA'G
Cuts at: 830 2373 2516 830
Size: 1543 143 3478
Fragments arranged by size:
3478 1543 143
PvuI CG_AT'CG
Cuts at: 2001 2064 2001
Size: 63 5101
SalI G'TCGA_C
Cuts at: 1936 2506 1936
Size: 570 4594
SbfI CC_TGCA'GG
Cuts at: 2516 2516
Size: 5164
ScaI AGT'ACT
Cuts at: 3412 5124 3412
Size: 1712 3452
SfcI C'TryA_G
Cuts at: 826 1045 2369 2512 3647 4328 4519 826
Size: 219 1324 143 1135 681 191 1471
Fragments arranged by size:
1471 1324 1135 681 219 191 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5164
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5164
SmaI CCC'GGG
Cuts at: 1872 2147 2491 1872
Size: 275 344 4545
Fragments arranged by size:
4545 344 275
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3268 4169 4431 820
Size: 197 926 1325 901 262 1553
Fragments arranged by size:
1553 1325 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2955 493
Size: 2462 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5164
SphI G_CATG'C
Cuts at: 1132 2522 1132
Size: 1390 3774
SspI AAT'ATT
Cuts at: 5 52 3088 5
Size: 47 3036 2081
Fragments arranged by size:
3036 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5164
StyI C'CwwG_G
Cuts at: 513 1065 2004 2603 513
Size: 552 939 599 3074
Fragments arranged by size:
3074 939 599 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3191 3208 3361 3546 3991 325
Size: 1255 1611 17 153 185 445 1498
Fragments arranged by size:
1611 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3410 5122 96
Size: 274 80 33 51 1443 1433 1712 138
Fragments arranged by size:
1712 1443 1433 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5124 5156 5124
Size: 32 5132
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5164
XbaI T'CTAG_A
Cuts at: 2500 2500
Size: 5164
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5164
XmaI C'CCGG_G
Cuts at: 1870 2145 2489 1870
Size: 275 344 4545
Fragments arranged by size:
4545 344 275
XmnI GAAnn'nnTTC
Cuts at: 3293 3293
Size: 5164
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2972 276
Size: 53 83 186 936 1438 2468
Fragments arranged by size:
2468 1438 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI